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A selenium-oxidizing rhizobia t3f4 with good colonization ability and its application

A rhizobia and selenium oxide technology, applied in the field of agricultural microorganisms, can solve the problems of difficult plant absorption and utilization, achieve strong sustainability, promote selenium enrichment in plants, and promote the effect of selenium absorption in plants

Active Publication Date: 2020-11-24
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soil contains elemental selenium, selenite, selenate and organic selenium, etc. Plants mainly absorb selenite and selenate, but selenite is easily absorbed and retained by the soil, making it difficult for plants to absorb and utilize

Method used

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  • A selenium-oxidizing rhizobia t3f4 with good colonization ability and its application
  • A selenium-oxidizing rhizobia t3f4 with good colonization ability and its application
  • A selenium-oxidizing rhizobia t3f4 with good colonization ability and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Isolation and Identification of Rhizobium sp. T3F4

[0033] (1) Sample collection: The applicant collected the selenium-rich soil sample used in the present invention from the surface soil in the selenium-rich area of ​​Enshi Tujia and Miao Autonomous Prefecture, Hubei Province in July 2012.

[0034] (2) Separation and purification of bacteria: Weigh 10g of soil sample into a conical flask filled with 90ml of sterile normal saline, shake it in a shaker at 28°C for half an hour, then take 1ml and add it to 9ml of sterile normal saline to gradually dilute to 10 -2 , 10 -3 , 10 -4 , 10 -5 , respectively take 0.1ml and apply it to 1 / 5LB solid medium, and apply 3 plates for each gradient. Culture at a constant temperature of 28°C for one week, pick a single colony and streak for purification. After purification, store it in a screw tube with 50% glycerol:bacteria solution (volume ratio 1:1), and store it at -80°C.

[0035] (3) Screening of selenium-oxidizing...

Embodiment 2

[0042] Embodiment 2: Rhizobium T3F4 oxidation elemental selenium curve

[0043] The specific steps are as follows:

[0044] (1) Prepare 3 bottles of sterilized 1 / 5LB liquid medium, 100ml in each bottle. Get three bottles and add 0.5g / L sterilized elemental selenium respectively. Inoculation log phase (OD 600 Value is about 0.8) T3F4 bacterial liquid, inoculum size 1%. Immediately after mixing, take a 2ml sample, which is taken as the first sample, and placed in a clean centrifuge tube. Each Erlenmeyer flask was placed in a constant temperature shaker at 28° C. and 160 rpm for cultivation. The sampling time was 0d, 1d, 2d, 4d, 5d, 6d, respectively, and the experiment was repeated three times. After sampling, the samples were processed as follows.

[0045] (2) Take 2ml of samples each time, centrifuge at 12000rpm for 10min, pass the supernatant through a 0.22μm filter membrane and store in a -20°C refrigerator.

[0046] (3) The atomic fluorescence speciation analyzer (mod...

Embodiment 3

[0050] Rhizobium T3F4 promotes selenium enrichment in pakchoi and transformation of selenium in soil

[0051] The specific steps are as follows:

[0052] Table 1 Experimental grouping

[0053]

[0054] "+" means adding, "-" means not adding

[0055] Chinese cabbage: Sijiqing Chinese cabbage, purchased from Fengcheng Hangcheng Seed Industry Co., Ltd., Jiangxi Province

[0056] (1) Soil pretreatment: weigh the air-dried soil 2kg / pot, then apply sodium selenite or elemental selenium (5mg / kg, compound concentration), mix well, add water at a moisture content of 15%, and balance for 3 days;

[0057] (2) After the balance is completed, dilute the rhizobium T3F4 mother liquor preserved in normal saline with water, and evenly spray the rhizobium T3F4 bacterial solution on the soil surface to ensure that the bacterial content in the soil is >10 7 cfu / g, the soil was mixed well, and each treatment was repeated 12 times;

[0058] (3) Immediately transplant the Sijiqing pakchoi (ap...

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Abstract

The invention belongs to the technical field of agricultural microorganisms, and specifically discloses a selenium-oxidizing rhizobia T3F4 with good colonization ability and its application. The preservation number of the strain is CCTCC NO: M 2019057. The strain has the ability to oxidize elemental selenium. The strain was fluorescently labeled and inoculated in potting soil. At 21 days, the number of cells in the rhizosphere soil and cabbage leaves of the strain remained at 6.6 million / g (dry weight) and 20 10,000 / g (fresh weight), showing strong colonization ability. On the other hand, adding elemental selenium or sodium selenite to potted plants, T3F4 can increase the content of available selenium in the soil, and significantly increase the selenium content of various parts of pakchoi. Therefore, rhizobia T3F4 can be prepared as a plant selenium enrichment agent, which has a good application prospect in biopromoting plant selenium enrichment.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and relates to a selenium-oxidizing rhizobia T3F4 with good colonization ability and its application. The rhizobium is classified as Rhizobium sp., and the strain has excellent colonization ability in soil and plants , Increase the available selenium content in the soil, and promote selenium enrichment in plants. Background technique [0002] Selenium (Se) is located in the main group VI of the periodic table of elements. Selenium exists in four valence states of Se(-II), Se(0), Se(IV) and Se(VI) in nature. Se(-II) is mainly organic selenium, which is commonly found in organisms, such as selenocysteine ​​(SeCys), selenomethionine (SeMet), monomethylselenium (CH 3 -Se), dimethylselenium (CH 3 -Se-CH 3 ) and methylselenocysteine ​​(SeMeCys), etc. Se(IV) and Se(VI) are mainly inorganic selenium, mostly present in non-biological organisms, usually as oxyanions, such as selenit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14C05F11/08C12R1/41C09K101/00
CPCC05F11/08C09K17/14C09K2101/00C12N1/205C12R2001/41
Inventor 郑世学王革娇朱达辉樊可可张富军
Owner HUAZHONG AGRI UNIV
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