Method for determining selenomethionine in biological matrix
A technology of selenomethionine and methylselenocysteine, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to identify SeMet, different flow rates, and inability to determine the original form of SeMet, etc., and achieve accurate analysis speed Reliable, easy-to-operate, high-sensitivity results
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[0042] (1) Inspection conditions and methods
[0043] 1. Preparation of standard samples
[0044] Accurately weigh an appropriate amount of SeMet, add water to dissolve, and obtain a SeMet standard stock solution with a concentration of 1 mg / ml, accurately measure an appropriate amount of SeMet standard stock solution, and perform serial dilution with water to obtain a concentration of 20, 50, 100, 200, 800, 1000, 1500, 2000ng / ml series of standard working solutions, then accurately measure 90μl of rat blank plasma, and precisely add 10μl of SeMet working solutions of different concentrations to obtain 2, 5, 10, 20, 80, 100, 150, 200ng / ml standards sample.
[0045] 2. Preparation of Quality Control Products
[0046] Precisely measure 10 μl of SeMet working solution of appropriate concentration, and then precisely measure 90 μl of rat blank plasma to obtain 3 concentrations of SeMet of 5, 80, and 150 ng / ml as quality control.
[0047] 3. Sample pretreatment: Accurately measu...
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