Method for determining selenomethionine in biological matrix

A technology of selenomethionine and methylselenocysteine, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to identify SeMet, different flow rates, and inability to determine the original form of SeMet, etc., and achieve accurate analysis speed Reliable, easy-to-operate, high-sensitivity results

Pending Publication Date: 2018-12-18
ANGEL NUTRITECH CO LTD
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Problems solved by technology

[0007] The prior art problem solved by the present invention is: in the prior art, high performance liquid chromatography inductively coupled plasma mass spectrometry (HPLC-ICP-MS), gas chromatography mass spectrometry (GC-MS), capillary electrophoresis (CE ), but when measured by high-performance liquid chromatography inductively coupled plasma mass spectrometry (HPLC-ICP-MS), only the Se element can be quantitatively limited, and the original form of SeMet cannot be determined, especially in the bod

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  • Method for determining selenomethionine in biological matrix
  • Method for determining selenomethionine in biological matrix
  • Method for determining selenomethionine in biological matrix

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Embodiment 1

[0042] (1) Inspection conditions and methods

[0043] 1. Preparation of standard samples

[0044] Accurately weigh an appropriate amount of SeMet, add water to dissolve, and obtain a SeMet standard stock solution with a concentration of 1 mg / ml, accurately measure an appropriate amount of SeMet standard stock solution, and perform serial dilution with water to obtain a concentration of 20, 50, 100, 200, 800, 1000, 1500, 2000ng / ml series of standard working solutions, then accurately measure 90μl of rat blank plasma, and precisely add 10μl of SeMet working solutions of different concentrations to obtain 2, 5, 10, 20, 80, 100, 150, 200ng / ml standards sample.

[0045] 2. Preparation of Quality Control Products

[0046] Precisely measure 10 μl of SeMet working solution of appropriate concentration, and then precisely measure 90 μl of rat blank plasma to obtain 3 concentrations of SeMet of 5, 80, and 150 ng / ml as quality control.

[0047] 3. Sample pretreatment: Accurately measu...

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Abstract

The invention relates to the technical field of biological detection, in particular to a method for determining selenomethionine in a biological matrix. The method for determining selenomethionine ina biological matrix provided by the invention comprises steps of: (1) sample pretreatment: taking a biological matrix sample, adding internal standard methyl selenocysteine, then treating the biological matrix sample with a protein precipitation method, and centrifuging to obtain a supernatant; (2) content determination: injecting the supernatant in the step (1) into a chromatograph by using highperformance liquid chromatography tandem mass spectrometry (LC-MS/MS) chromatography to calculate the concentration of selenomethionine in the biological matrix sample. The extraction efficiency of selenomethionine is high, and the biological matrix does not interfere with the determination of selenomethionine by adopting the protein precipitation method in the biological matrix of the invention;and the speed is fast, the special property is good, the accuracy is high and the reproducibility is good by determining selenomethionine in the biological matrix with the LC-MS/MS chromatography.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for determining selenomethionine in a biological matrix. Background technique [0002] Selenium is an essential trace element for humans and animals. Selenium only exists in selenoproteins in the form of selenomethionine (SeMet) and selenocysteine, and most of selenium is SeMet. Selenium exerts its biological effects by affecting free radical metabolism, antioxidant function, immune function, reproductive function, cell apoptosis and endocrine hormones in the body through selenoproteins. More than 40 cases of Keshan disease, Kaschin-Beck disease, diabetes and AIDS This disease is related to selenium deficiency. Therefore, the content of SeMet in the organism is an important physiological index reflecting the level of active selenium in the body. [0003] There are more than 40 countries and regions in the world suffering from selenium deficiency in varying...

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Application Information

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IPC IPC(8): G01N30/02G01N30/14
CPCG01N30/02G01N30/14G01N2030/045
Inventor 张双庆俞学锋李知洪姚鹃张彦张海波彭宁陈智仙
Owner ANGEL NUTRITECH CO LTD
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