Method for promoting starting of anaerobic digestor and accelerating conversion of propionic acid into methane

An anaerobic digestion system and digester technology, which is applied in the field of promoting the start of anaerobic digesters and accelerating the conversion of propionic acid to methane, can solve the problems of harsh nutritional conditions of syntrophic bacteria, limit fatty acid metabolism, slow growth, etc., and achieve anti-retrograde Strong, promotes transport metabolism, and promotes the effect of operating efficiency

Inactive Publication Date: 2017-01-25
BIOGAS SCI RES INST MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Intertrophic metabolism of microorganisms is the rate-limiting step in the process of methanogenesis in the degradation of organic matter. The intertrophic bacteria involved in this metabolism have harsh nutritional conditions, low energy acquisition, and slow growth, which greatly limits the metabolism of fatty acids.

Method used

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  • Method for promoting starting of anaerobic digestor and accelerating conversion of propionic acid into methane
  • Method for promoting starting of anaerobic digestor and accelerating conversion of propionic acid into methane
  • Method for promoting starting of anaerobic digestor and accelerating conversion of propionic acid into methane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Prepare anaerobic BCTY culture solution, add sodium propionate with a final concentration of 3000mg / L, aliquot 150ml into a 250ml anaerobic bottle, inoculate 10% (V / V) dextran wastewater anaerobic digester effluent, seal, 40°C static culture. When the degradation rate of propionic acid reaches 80%, and the methane content is about 50%, add functional strains (cells / ml culture solution) according to the following three schemes:

[0034] Option 1: Pelotomaculum schinkii 2.5×10 7 , Pelotomaculum propionicicum 1×10 7 , Syntrophobacter wolinii 1×10 6 , Methanospirillum hungatei 4×10 6 , Methanoculleus palmolei 5 x 10 6 , Methanoculleus bourgensis 5×10 6, Methanosarcina barkeri 5 x 10 6 , Methanosarcina mazei 1×10 7 .

[0035] Solution 2: Pelotomaculum schinkii 4×10 7 , Pelotomaculum propionicicum 1.5×10 7 , Syntrophobacter wolinii 3×10 6 , Methanospirillum hungatei 1.4×10 7 , Methanoculleus palmolei 1 x 10 7 , Methanoculleus bourgensis 1×10 7 , Methanosarcinaba...

Embodiment 2

[0038] Embodiment 2 Composite flora is to the adaptability of pH

[0039] Prepare anaerobic BCTY culture solution, add sodium propionate with a final concentration of 3000mg / L, aliquot 150ml into a 250ml anaerobic bottle, inoculate 20% (V / V) dextran wastewater anaerobic digester effluent, seal, 20°C static culture. When the degradation rate of propionic acid reaches 70%, and the methane content is about 40%, add functional strains (cells / ml culture fluid) according to the following scheme: Pelotomaculum shinkii 5×10 7 , Pelotomaculum propionicum 2.5×10 7 , Syntrophobacter wolinii 3×10 6 , Methanospirillum hungatei 1.4×10 7 , Methanoculleus palmolei 1.4×10 7 , Methanoculleus bourgensis 1.4×10 7 , Methanosarcina barkeri 2.5×10 7 , Methanosarcina mazei 3×10 7 .

[0040] Seal it and culture it statically at 20°C to obtain the methanogenic complex flora that hydrolyzes propionic acid. Inoculate the obtained flora into pH6.0, pH 7.0, pH 8.0, and pH 9.0 fresh BCTY medium res...

Embodiment 3

[0041] Embodiment 3 composite flora is to the metabolizing ability of sodium propionate

[0042] Prepare anaerobic BCTY culture solution, add sodium propionate with a final concentration of 10000mg / L, aliquot 150ml into a 250ml anaerobic bottle, inoculate 1% (V / V) dextran wastewater anaerobic digester effluent, seal, 55°C static culture. When the degradation rate of propionic acid reaches 50%, and the methane content is about 30%, add functional strains (cells / ml culture fluid) according to the following scheme: Pelotomaculum shinkii 5×10 7 , Pelotomaculum propionicum 2.5×10 7 , Syntrophobacter wolinii 3×10 6 , Methanospirillum hungatei 1.4×10 7 , Methanoculleus palmolei 1.4×10 7 , Methanoculleus bourgensis 1.4×10 7 , Methanosarcina barkeri 2.5×10 7 , Methanosarcina mazei 3×10 7 .

[0043] Seal it, and culture it statically at 55°C to obtain the methanogenic complex flora that hydrolyzes propionic acid. The obtained flora was inoculated in fresh BCTY medium with pH 7....

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Abstract

The invention provides a method for promoting the starting of an anaerobic digestor and accelerating the conversion of propionic acid into methane. The method comprises the steps of preparing compound bacteria, adding a solid carrier into the compound bacteria in a mass percentage of 1%-20%, inoculating the compound bacteria into a sterile anaerobic BCTY culture medium containing 1500ppm-10000ppm of sodium propionate, and carrying out anaerobic standing culturing at 40 DEG C, wherein the pH value of the sterile anaerobic BCTY culture medium is 7.5-8.5. By virtue of the method, the degradation rate of propionic acid can be substantially increased, and the time required for the complete degradation of propionic acid can be substantially shortened; the operation efficiency of the metabolic conversion of propionic acid into methane is remarkably promoted; and the transport metabolism of H2 in an anaerobic digestion system can be effectively promoted, so that the anaerobic digestion system is maintained at a relatively low hydrogen partial pressure state, is relatively stable and has relatively strong inversion resistance, and the operation load is substantially improved.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, in particular to a method for promoting the start-up of an anaerobic digester and accelerating the conversion of propionic acid into methane. Background technique [0002] In the anaerobic digestion system, the accumulation of organic acids such as acetic acid, propionic acid, butyric acid often leads to the acidification of the anaerobic digestion system, the reduction of organic load and even the collapse of the whole system. However, as important intermediate metabolites of anaerobic transformation of organic waste, acetic acid, propionic acid, and butyric acid are difficult to degrade. Short-chain fatty acids (such as ethyl, propionate, and butyric acid) are important intermediate metabolites in the biogas fermentation process, and their metabolic transformation is an important link in controlling the biogas fermentation process. However, under standard conditions, the anaerobic oxida...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P5/02C02F11/04
CPCC02F11/04C12P5/023C12P39/00Y02E50/30Y02P20/59
Inventor 黄艳马诗淳凡慧邓宇张敏施国中王春芳尹小波
Owner BIOGAS SCI RES INST MIN OF AGRI
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