37 results about "CYP2C9" patented technology

The invention relates to a medicine metabolic relevant loci detection method, which comprises the following steps: extracting genome DNA from human samples; respectively designing a Taqman probe pair and a primer pair according to at least two medicine metabolic relevant genes; respectively marking the 5' end and the 3'end of the Taqman probe pair with fluorescence reporting genes and fluorescence quenching genes; carrying out fluorescence quantitative PCR augmentation on the genome DNA; and judging whether the medicine metabolic relevant genes have the mutation according to the fluorescence quantitative PCR augmentation results. Preferably, the number of the medicine metabolic relevant genes is four, the Taqman probe pair and the primer pair are used for detecting a loci rs1057910 of a gene CYP2C9, a loci rs4244285 of a gene CYP2C19, a loci rs4986893of a gene CYP2C19, a loci rs1065852 of a gene CYP2D6 and a loci rs28371759 of a gene CYP3A4. The invention has the advantages of ingenious design, simple operation and accurate and reliable detection results, and provides the reference frame for determining whether professional doctors are needed to be consulted so as to make sure themedicine can be taken or not or the proper dosage and the like when a certain medicine is taken.

A near-infrared fluorescent probe for detecting cytochrome P450 2C9 and its application belong to the technical field of biomedicine. The specific probe substrate can be used to measure the enzymatic activity of CYP2C9 in biological systems. The process of CYP2C9 enzyme activity determination is as follows: 1,3-dichloro-7-alkoxy-9,9-dimethyl-2(9H)-acridone was selected O The dealkylation reaction is a probe reaction, which quantitatively detects the elimination amount of the substrate 1,3-dichloro-7-alkoxy-9,9-dimethyl-2(9H)-acridone in unit time The activity of CYP2C9 enzymes in various biological samples was determined by the amount of its dealkylated metabolites. The invention can be used for quantitative assessment of CYP2C9 enzyme activity in biological samples from different sources, species and individuals and imaging of CYP2C9 in organisms, so as to realize the assessment of CYP2C9's ability to dispose of drugs. It can also be used for rapid in vitro screening of CYP2C9 activity modulators, and to evaluate the potential of drug-drug interactions due to inhibition or activation of CYP2C9 catalytic activity, such as drugs and candidate compounds.

The invention discloses a specific primer and liquid phase chip for SNP (Single Nucleotide Polymorphism) detection of CYP2C9 (Cytochrome P4502C9), wherein the liquid phase chip comprises an ASPE primer consisting of a tag sequence of 5' end and a specific primer of 3' end aiming at target gene mutation, a microsphere coated by the anti-tag sequence, and an amplification primer; the specific primer includes: SEQ ID NO.17 and SEQ ID NO.18 aiming at G98A SNP locus, SEQ ID NO.19 and SEQ ID NO.20 aiming at G173A SNP locus, SEQ ID NO.21 and SEQ ID NO.22 aiming at C90T SNP locus, SEQ ID NO.23 and SEQ ID NO.24 aiming at A162C SNP locus, SEQ ID NO.25 and SEQ ID NO.26 aiming at C72T SNP locus, SEQ ID NO.27 and SEQ ID NO.28 aiming at T102C SNP locus, SEQ ID NO.29 and SEQ ID NO.30 aiming at A77G SNP locus, and / or SEQ ID NO.31 and SEQ ID NO.32 aiming at A167C SNP locus. The matching rate between a detection result obtained by adopting the liquid phase chip for SNP detection of CYP2C9 and a sequencing method can be 100%.

The invention belongs to the field of biology, relates to single-base mutation, and in particular relates to a CYP2C9 gene which is corresponding to the mutation site mutated from wild type T into C on the 1400th bit of SEQ ID NO.2, a nucleic acid segment containing the mutation site, a protein segment coded by the nucleic acid segment and application thereof. The invention also provides allele specific oligonucleotide, a kit and a detection method for detecting the mutation site.

The invention relates to a method for detecting CYP2C9 enzyme activity in earthworms by adopting a high-performance liquid chromatography-tandem mass spectrometry and belongs to the technical field of enzyme activity detection. The method specifically comprises the following steps: (1) preparing earthworm microsome protein suspension; (2) adding the earthworm microsome protein suspension prepared in the step (1) into an incubation system containing a probe substrate; raising the temperature to start an enzymatic reaction; after the enzymatic reaction is stopped, detecting a metabolic product, which is generated after the enzymatic reaction is stopped, by adopting the high-performance liquid chromatography-tandem mass spectrometry; calculating the CYP2C9 enzyme activity. The detection method is high in accuracy, precision and sensitivity and high in stability, and can be used for analyzing and detecting the CYP2C9 enzyme activity in the earthworms under different pollution environments, so that a detection method for probing the possibility of taking CYP2C9 as a biological marker to indicate soil pollution is provided.