Specific primer and liquid phase chip for SNP (Single Nucleotide Polymorphism) detection of CYP2C9 (Cytochrome P4502C9)

A detection solution and specific technology, applied in the field of molecular biology, can solve the problems that gene mutation detection cannot be used, cannot meet practical application, and is prone to cross-reaction, etc., and achieves good signal-to-noise ratio, consistent detection effect, and detection specificity. good effect
CN102154455AActive Publication Date: 2011-08-17SUREXAM BIO TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUREXAM BIO TECH
Publication Date
2011-08-17

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Abstract

The invention discloses a specific primer and liquid phase chip for SNP (Single Nucleotide Polymorphism) detection of CYP2C9 (Cytochrome P4502C9), wherein the liquid phase chip comprises an ASPE primer consisting of a tag sequence of 5' end and a specific primer of 3' end aiming at target gene mutation, a microsphere coated by the anti-tag sequence, and an amplification primer; the specific primer includes: SEQ ID NO.17 and SEQ ID NO.18 aiming at G98A SNP locus, SEQ ID NO.19 and SEQ ID NO.20 aiming at G173A SNP locus, SEQ ID NO.21 and SEQ ID NO.22 aiming at C90T SNP locus, SEQ ID NO.23 and SEQ ID NO.24 aiming at A162C SNP locus, SEQ ID NO.25 and SEQ ID NO.26 aiming at C72T SNP locus, SEQ ID NO.27 and SEQ ID NO.28 aiming at T102C SNP locus, SEQ ID NO.29 and SEQ ID NO.30 aiming at A77G SNP locus, and / or SEQ ID NO.31 and SEQ ID NO.32 aiming at A167C SNP locus. The matching rate between a detection result obtained by adopting the liquid phase chip for SNP detection of CYP2C9 and a sequencing method can be 100%.
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Description

technical field

[0001] The invention belongs to the field of molecular biology, relates to medicine and biotechnology, in particular to a CYP2C9 gene SNP detection specific primer and a liquid phase chip. Background technique

[0002] The main enzymes that metabolize drugs in the human body are the cytochrome P450 superfamily (Cytochrome P450 proteins, CYP). Metabolism of xenobiotics (including most clinical drugs). P450 enzymes can transfer electrons to oxidize heterologous substances through iron ions in non-covalently bonded heme, enhance the water solubility of heterologous substances, and make them easier to excrete.

[0003] There are multiple subfamilies of CYP, among which CYP2C9 (Cytochrome P4502C9) is an important member of the second subfamily, accounting for 20% of the total amount of P450 proteins in liver microsomal. CYP2C9 can hydroxylate and metabolize many drugs with different properties, mainly acidic substrates. According to statistics, about 16% of cli...

Claims

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