133results about How to "Sensitive and specific recognition" patented technology

The invention discloses an NRAS gene mutation detection specificity primer and a liquid chip thereof. The liquid chip mainly comprises: an ASPE primer composed of a 5'-terminal tag sequence and 3'-terminal specificity primer sequences focused on target gene mutation sites, wherein the specificity primer sequences comprise SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21 and/or SEQ ID NO.22 focused on a Codon12 site, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26 and/or SEQ ID NO.27 focused on a Codon13 site, SEQ ID NO.28 and SEQ ID NO.29 focused on a Codon18 site, and/or SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33 and/or SEQ ID NO.34 focused on a Codon61 site; a microsphere coated by an anti-tag sequence; and an amplimer. The consistency between the detection result of the detection liquid chip provided by the invention and the detection result of a sequencing method is high to 100%, and the wild-type and mutant parallel detection of a plurality of mutation sites is realized.

The invention provides a monoclonal antibody targeting at Zika virus envelop protein conserved epitope and application thereof. specifically, a recombinant expressed Zika virus E-protein immune mouseis used herein to attain a murine monoclonal antibody specific to E-protein. Research results show that the antibody herein is an ideal Zika virus detection antibody and is applicable to the development antibody drugs against Zika virus. Identification of a conserved neutralizing epitope can also guide the development of broad-spectrum Zika virus vaccines.

The invention discloses a THADA gene mutation detection liquid phase chip and a specific primer. The liquid phase chip mainly comprises: an ASPE primer composed of a tag sequence at 5'-terminal and specific primer sequences against the mutation site of a target gene at 3'-terminal, wherein the specific primer sequences comprise SEQ ID NO.13 and SEQ ID NO.14 against an A109G site, SEQ ID NO.15 and SEQ ID NO.16 against a T156C site, SEQ ID NO.17 and SEQ ID NO.18 against an A89C site, SEQ ID NO.19 and SEQ ID NO.20 against a G153A site, SEQ ID NO.21 and SEQ ID NO.22 against a G162T site, and/or SEQ ID NO.23 and SEQ ID NO.24 against a C80T site; a microsphere coated with an anti-tag sequence; and an amplimer. The coincidence rate between the detection result of the detection liquid phase chip and a sequencing method is 100%, and the wild and mutant parallel detection of a plurality of mutation sites can be realized.

The invention discloses an ATM gene mutation detection specific primer and a liquid chip. The liquid chip mainly comprises ASPE primers consisting of tag sequences at a 5' end and specific primer sequences aiming at target gene mutation sites at a 3' end, microspheres coated with anti-tag sequences and an amplification primer, wherein the specific primer sequences are as follows: SEQ ID NO.13 and SEQ ID NO.14 which aim at a T2572C site, SEQ ID NO.15 and SEQ ID NO.16 which aim at an A7325C site, SEQ ID NO.17 and SEQ ID NO.18 which aim at a G7328A site, SEQ ID NO.19 and SEQ ID NO.20 which aim at a C9022T site, SEQ ID NO.21 and SEQ ID NO.22 which aim at a G9023A site, and/or SEQ ID NO.23 and SEQ ID NO.24 which aim at a C9139T site. The detection liquid chip provided by the invention has the advantages that the matching rate between the detection result and a sequencing method is up to 100%, and the wild-type and mutant parallel detection of multiple mutant sites can be realized.

The invention discloses a BAT3 gene mutation detection liquid phase chip and a specific primer. The liquid phase chip mainly comprises: an ASPE primer composed of a tag sequence at 5'-terminal and specific primer sequences against the mutation site of a target gene at 3'-terminal, wherein the specific primer sequences comprise SEQ ID NO.1 and SEQ ID NO.2 against an A131C site, and/or SEQ ID NO.3 and SEQ ID NO.4 against a G96A site; a microsphere coated with an anti-tag sequence; and an amplimer. The coincidence rate between the detection result of the detection liquid phase chip and a sequencing method is 100%, and the wild and mutant parallel detection of a plurality of mutation sites can be realized.

The invention discloses specific primers and a liquid phase chip for detecting the polymorphism of a CDKAL1 gene. The liquid phase chip mainly comprises specific primers consisting of a tag sequence at a 5' end and specific primer sequences of polymorphic sites of a target gene, microspheres and amplification primers, wherein the specific primer sequences are one or more pairs from SEQ ID No.9 and SEQ ID No.10 for A107C, SEQ ID No.11 and SEQ ID No.12 for G323C, SEQ ID No.13 and SEQ ID No.14 for A210G and SEQ ID No.15 and SEQ ID No.16 for A75G; the tag sequence may be a sequence from SEQ ID No.1 to SEQ ID No.8. The prepared liquid phase chip for detecting the polymorphism of the CDKAL1 gene has a very high signal-to-noise ratio, and the cross reaction of a designed probe and an anti-tae sequence is prevented basically.

The invention discloses a VHL (Von Hippel Lindau) genetic mutation detection specific primer and a liquid phase chip. The liquid phase chip mainly comprises ASPE (Allele Specific Primer Extension) primers, microspheres coated by an anti-tag sequence, and amplimers, wherein the ASPE primers consist of tag sequences at a 5' end and specific primer sequences specific to target genetic mutation sites at a 3' end; and the specific primer sequences are: SEQ ID NO.13 and SEQ ID NO.14 specific to a T240A site; SEQ ID NO.15 and SEQ ID NO.16 specific to a T254C site; SEQ ID NO.17 and SEQ ID NO.18 specific to a T266A site; SEQ ID NO.19 and SEQ ID NO.20 specific to a T286T site; SEQ ID NO.21 and SEQ ID NO.22 specific to a G388C site; and/or SEQ ID NO.23 and SEQ ID NO.24 specific to a 444delT site. The matching rate between a detection result of the liquid phase chip provided by the invention and a sequencing method is up to 100 percent, and wild and mutant parallel detection of a plurality of mutant sites is realized.

The invention discloses an ABCC (ATP (adenosine triphosphate)-binding cassette, sub-family C)1 gene mutation detection liquid chip and specific primers. The liquid chip mainly comprises each ASPE (allele specific primer extension) primer formed by tag sequences at 5' terminal and specific primer sequences aiming at target gene mutation sites at 3' terminal, microspheres coated by anti-tag sequences as well as amplification primers, wherein the specific primer sequences are SEQ ID NO.15 and SEQ ID NO.16 aiming at A866T sites, SEQ ID NO.17 and SEQ ID NO.18 aiming at C218T sites, SEQ ID NO.19 and SEQ ID NO.20 aiming at G2168A sites, SEQ ID NO.21 and SEQ ID NO.22 aiming at G3173A sites, SEQ ID NO.23 and SEQ ID NO.24 aiming at G16237754A sites, SEQ ID NO.25 and SEQ ID NO.26 aiming at C16238494T sites and/or SEQ ID NO.27 and SEQ ID NO.28 aiming at G1299T sites. The liquid chip has the advantages that the coincidence rate of the detection results of the detection liquid chip provided by the invention and a sequencing method is as high as 100%; and parallel detection of wild types and mutant types of a plurality of mutation sites is achieved.

The invention discloses a XRCC2 gene mutation detection liquid chip, and specific primers. The XRCC2 gene mutation detection liquid chip mainly comprises: ASPE primers, wherein each ASPE primer is composed of 5'-terminal tag sequence, and 3'-terminal specific primer sequence targeting target gene mutation sites, and the specific primer sequence comprises SEQ ID No.9 and SEQ ID No.10 targeting G87A site, SEQ ID No.11 and SEQ ID No.12 targeting G158C site, SEQ ID No.13 and SEQ ID No.14 targeting A82G site, and/or SEQ ID No.15 and SEQ ID No.16 targeting A155C site; microballoons coated with anti-tag sequences; and amplification primers. Self-agreement ratio of detection results of the liquid chip with detection results of sequencing is as high as 100%; and parallel detection of wild types and mutant types of a plurality of mutation sites is realized.

The present invention discloses a detection liquid phase chip and specific primers for FYCO1 gene mutation. The liquid phase chip mainly comprises: ASPE primers comprising a 5' terminal tag sequence and a 3' terminal target gene mutational site-targeted specific primer sequence, wherein the specific primer sequence comprises A92G site-targeted SEQ ID NO.1, A92G site-targeted SEQ ID NO.2, and/or C57T site-targeted SEQ ID NO.3 and C57T site-targeted SEQ ID NO.4; anti-tag sequence coated microspheres; and amplification primers. According to the present invention, coincidence frequency of the detection results of the detection liquid phase chip and the sequencing method is up to 100%, and single and parallel detection on the wild-type with multiple mutational sites and the mutant-type with multiple mutational sites can be achieved.

The invention discloses CYP2C8 gene detection specific primers and a liquid chip. The liquid chip mainly comprises: various ASPE primers composed of 5' terminal tag sequence and 3' terminal specific primer sequences aiming at target gene mutation site, microspheres coated with anti-tag sequence, and amplification primers. The specific primer sequences comprise: SEQ ID NO.13 and SEQ ID NO.14 aiming at A127T site; SEQ ID NO.15 and SEQ ID NO.16 aiming at A124G site; SEQ ID NO.17 and SEQ ID NO.18 aiming at G145A site; SEQ ID NO.19 and SEQ ID NO.20 aiming at 204delA site; SEQ ID NO.21 and SEQ ID NO.22 aiming at C115G site; and/or SEQ ID NO.23 and SEQ ID NO.24 aiming at G315C site. The matching rate of the detection result obtained by using the liquid chip provided by the invention and a result obtained by using a sequencing method is 100%. Therefore, wild-type and mutant-type parallel detections of a plurality of mutation sites are realized.

The invention relates to the technical field of electrochemical sensing, and discloses a 4-chlorophenol molecularly imprinting electrochemical sensor and a preparation method thereof. According to thepreparation method, 4-chlorophenol is taken as a template molecule, dopamine hydrochloride is taken as a functional monomer, and the 4-chlorophenol molecularly imprinted polymer loaded with the Cu@Cu2O composite nano porous material is prepared through adoption of a simple one-pot method. The 4-chlorophenol molecularly imprinted polymer loaded with the Cu@Cu2O composite nano porous material is combined with an electrochemical sensor in a dispensing manner to construct the 4-chlorophenol molecularly imprinted electrochemical sensor, and the sensor realizes rapid and sensitive specific recognition of 4-chlorophenol and can be used for detection of an actual water sample.

The invention discloses CYP1A1 gene detection specific primers and a liquid chip. The liquid chip mainly comprises: various ASPE primers composed of 5' terminal tag sequence and 3' terminal specific primer sequences aiming at target gene mutation site, microspheres coated with anti-tag sequence, and amplification primers. The specific primer sequences comprise: SEQ ID NO.17 and SEQ ID NO.18 aiming at T109C site; SEQ ID NO.19 and SEQ ID NO.20 aiming at T97C site; SEQ ID NO.21 and SEQ ID NO.22 aiming at 133-134insT site; SEQ ID NO.23 and SEQ ID NO.24 aiming at T201A site; SEQ ID NO.25 and SEQ ID NO.26 aiming at C240A site; SEQ ID NO.27 and SEQ ID NO.28 aiming at A242G site; SEQ ID NO.29 and SEQ ID NO.30 aiming at C248A site; and/or SEQ ID NO.31 and SEQ ID NO.32 aiming at G135T site. The matching rate of the detection result obtained by using the liquid chip provided by the invention and a result obtained by using a sequencing method is 100%. Therefore, wild-type and mutant-type parallel detections of a plurality of mutation sites are realized.

The invention discloses CYP2B6 gene detection specific primers and a liquid chip. The liquid chip mainly comprises: various ASPE primers composed of 5' terminal tag sequence and 3' terminal specific primer sequences aiming at target gene mutation site, microspheres coated with anti-tag sequence, and amplification primers. The specific primer sequences comprise: SEQ ID NO.13 and SEQ ID NO.14 aiming at C123T site; SEQ ID NO.15 and SEQ ID NO.16 aiming at A101G site; SEQ ID NO.17 and SEQ ID NO.18 aiming at G118T site; SEQ ID NO.19 and SEQ ID NO.20 aiming at A77G site; SEQ ID NO.21 and SEQ IDNO.22 aiming at T139C site; and/or SEQ ID NO.23 and SEQ ID NO.24 aiming at C288T site. The matching rate of the detection result obtained by using the liquid chip provided by the invention and a result obtained by using a sequencing method is 100%. Therefore, wild-type and mutant-type parallel detections of a plurality of mutation sites are realized.

The invention discloses an interleukin-23 receptor (IL-23R) gene polymorphism detection specific primer and an IL-23R gene polymorphism detection liquid phase chip. The liquid phase chip mainly comprises an ASPE primer, microspheres and an amplified primer, wherein the ASPE primer consists of a tag sequence of a 5' end and specific primers of a 3' end, and the specific primers are selected from more than one pair of SEQ ID NO.9 and SEQ ID NO.10 aiming at C214T, SEQ ID NO.11 and SEQ ID NO.12 aiming at C235T, SEQ ID NO.13 and SEQ ID NO.14 aiming at T82G, and SEQ ID NO.15 and SEQ ID NO.16 aimingat C53T. The IL-23R gene polymorphism detection liquid phase chip has very good signal-noise ratio, the designed probe and an anti-tag sequence basically have no cross reaction, and the selection of a tag sequence and the anti-tag sequence and the combination of the tag sequence and the specific ASPE primer can avoid cross reaction and realize parallel detection of multiple polymorphic sites.