133results about How to "Sensitive and specific recognition" patented technology

The invention discloses a specific primer and liquid chip for detecting polymorphism of a DPYD (dihydropyrimidine dehydrogenase) gene. The liquid chip mainly comprises ASPE (allele specific primer extension) primers, microspheres and amplification primers, wherein each ASPE primer is formed by tag sequences at the 5' terminal and specific primer sequences which are arranged at the 3' terminal and aim at the mutation site of the target gene; the specific primer sequences are SEQ ID NO.7 and SEQ ID NO.8 aiming at the G55A site, SEQ ID NO.9 and SEQ ID NO.10 aiming at the G141A site and / or SEQ ID NO.11 and SEQ ID NO.12 aiming at the G134T site; and the microspheres are enveloped by anti-tag sequences. The detecting liquid chip provided by the invention has the advantages that the consistency of the detection result and the sequencing method is as high as 100%, thus parallel detection of wild types and mutant types of a plurality of mutation sites is achieved.

The invention discloses specific primers and a liquid-phase chip for the single nucleotide polymorphism (SNP) detection of the kit ligand gene (KITLG). The liquid-phase chip mainly comprises allele specific primers (ASPE), each of which consists of a tag sequence at a 5' end and specific primers aiming at mutational sites of target genes at a 3' end, microspheres wrapped by an anti-tag sequence and amplification primers, wherein sequences of the specific primers are one or more from the following pairs of sequences: SEQ ID No. 19 and SEQ ID No. 20, SEQ ID No. 21 and SEQ ID No. 22, SEQ ID No. 23 and SEQ ID No. 24, SEQ ID No. 25 and SEQ ID No. 26, SEQ ID No. 27 and SEQ ID No. 28, SEQ ID No. 29 and SEQ ID No. 30, SEQ ID No. 31 and SEQ ID No. 32, SEQ ID No. 33 and SEQ ID No. 34 and SEQ ID No.35 and SEQ ID No. 36. In the detection liquid-phase chip, the coincidence rate of detection results and a sequencing method is up to 100 percent, so the parallel detection of the wild type and mutanttype of polymorphic loci is realized.

The invention discloses a single-nucleotide polymorphism (SNP) detection specific primer of the CYP2C19 gene and a liquid phase chip. The liquid phase chip contains an ASPE primer composed of a tag sequence on the 5'-end and specific primers on the 3'-end which aims at the target genetic mutation, microspheres covered by the anti-tag sequence and an amplification primer, wherein the specific primers contain SEQ ID NO.25 and SEQ ID NO.26, SEQ ID NO.27 and SEQ ID NO.28 aiming at the C194T SNP site, SEQ ID NO.29 and SEQ ID NO.30, SEQ ID NO.31 and SEQ ID NO.32, SEQ ID NO.33 and SEQ ID NO.34, SEQID NO.35 and SEQ ID NO.36, SEQ ID NO.37 and SEQ ID NO.38, SEQ ID NO.39 and SEQ ID NO.40, SEQ ID NO.41 and SEQ ID NO.42, SEQ ID NO.43 and SEQ IDNO.44, SEQ ID NO.45 and SEQ ID NO.46, and / or SEQ ID NO.47 and SEQ ID NO.48. The coincidence rate of the detection result of the SNP detection liquid phase chip of the CYP2C 19 gene and the detection result adopting a sequencing method is up to 100%.

The invention discloses a liquid chip and specific primer for detecting an SLC22A1 gene. The liquid chip mainly comprises ASPE (allele specific primer extension) primers, microspheres and amplification primers, wherein each ASPE primer is formed by tag sequences at the 5' terminal and specific primer sequences which are arranged at the 3' terminal and aim at the mutation site of the target gene; the specific primer sequences are SEQ ID NO.19 and SEQ ID NO.20 aiming at the C117T site, SEQ ID NO.21 and SEQ ID NO.22 aiming at the T198C site, SEQ ID NO.23 and SEQ ID NO.24 aiming at the C86T site, SEQ ID NO.25 and SEQ ID NO.26 aiming at the C97G site, SEQ ID NO.27 and SEQ ID NO.28 aiming at the C164T site, SEQ ID NO.29 and SEQ ID NO.30 aiming at the G123T site, SEQ ID NO.31 and SEQ ID NO.32 aiming at the G127A site, SEQ ID NO.33 and SEQ ID NO.34 aiming at the A148G site and / or SEQ ID NO.35 and SEQ ID NO.36 aiming at the G129C site; and the microspheres are enveloped by anti-tag sequences. The detecting liquid chip provided by the invention has the advantages that the consistency of the detection result and the sequencing method is as high as 100%, thus parallel detection of wild types and mutant types of a plurality of mutation sites is achieved.

The invention relates to a liquid phase chip for the polymorphic detection of an age-related maculopathy susceptibility 2 (ARMS2) gene and a high temperature factor A-1 (HTRA1) gene. The liquid phase chip comprises allele specific primer extension (ASPE) primers consisting of tag sequences at the 5' end and specific primers aiming at a mutant target gene at the 3' end, microspheres which are wrapped with anti-tag sequences and an amplification primer, wherein the specific primers are SEQ ID No. 10 and SEQ ID No. 11 for a G72T mutant locus of the ARMS2 gene, SEQ ID No. 12 to SEQ ID No. 14 for a C95G / A mutant locus of the HTRA1 gene, SEQ ID No. 15 and SEQ ID No. 16 for a G60A mutant locus of the HTRA1 gene and / or SEQ ID No. 17 and SEQ ID No. 18 for the insertion / deletion (I / D) mutation of the HTRA1 gene. The coincidence rate of a detection result of the liquid phase chip and a sequencing method is up to 100 percent, cross reaction can be prevented, and the parallel detection of multiplepolymorphic loci is realized.