108results about How to "Precise Quantitative Analysis Features" patented technology

The invention provides a CYP2D6 gene mutation detection liquid-phase chip which comprises ASPE (Allele Specific Primer Extension) primers aiming at CYP2D6 C2850T and CYP2D6Deletion mutational sites, three microballoons respectively enveloped with a specific anti-tag sequence and amplification primers aiming at the CYP2D6C2850T and the CYP2D6 Deletion mutational sites. The CYP2D6 gene mutation detection liquid-phase chip can simultaneously detect aiming at the CYP2D6 C2850T and the CYP2D Deletion mutational sites and has excellent signal to noise ratio. The coincidence ratio with a sequencing method of the CYP2D6 gene mutation detection liquid-phase chip reaches up to 100 percent, and the CYP2D6 gene mutation detection liquid-phase chip has higher specificity and precision compared with intra-class correlation products.

The invention discloses an NRAS gene mutation detection specificity primer and a liquid chip thereof. The liquid chip mainly comprises: an ASPE primer composed of a 5'-terminal tag sequence and 3'-terminal specificity primer sequences focused on target gene mutation sites, wherein the specificity primer sequences comprise SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21 and/or SEQ ID NO.22 focused on a Codon12 site, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26 and/or SEQ ID NO.27 focused on a Codon13 site, SEQ ID NO.28 and SEQ ID NO.29 focused on a Codon18 site, and/or SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33 and/or SEQ ID NO.34 focused on a Codon61 site; a microsphere coated by an anti-tag sequence; and an amplimer. The consistency between the detection result of the detection liquid chip provided by the invention and the detection result of a sequencing method is high to 100%, and the wild-type and mutant parallel detection of a plurality of mutation sites is realized.

The invention discloses a liquid phase chip for detecting EGFR (epidermal growth factor receptor) gene mutation. The liquid phase chip mainly comprises a wild-type and mutant-type allele specific primer extension (ASPE) primer pair designed for mutational sites of an EGFR gene respectively, microspheres which are respectively coated with specific anti-tag sequences and have different colors of codes and amplification primers for respectively amplifying the target sequences with the corresponding mutational sites of the Exon 19, Exon 20 and/or Exon 21, wherein each ASPE primer comprises the tag sequences at the 5' terminal and the specific primers aiming at the mutational sites of the target gene at the 3' terminal; and the anti-tag sequences can correspondingly complement and form pair with the tag sequences selected in (A). The liquid phase chip has the following advantages: the coincidence rate of the detection method provided by the invention and a sequencing method is as high as 100%; the prepared liquid phase chip has very good signal to noise ratio; and cross reactions do not exist between the designed probes and the anti-tag sequences.

The invention discloses an SNP (Single Nucleotide Polymorphism) detection liquid phase chip for KIF6 gene, mainly comprising an ASPE (Application Solid Phase Extraction) primer pair, microballons and an amplification primer, wherein each ASPE primer consists of a 5'-end tag sequence and 3'-end specific primers aiming at T2155C SNP locus; the 3'-end specific primers comprise SEQID NO.23 and SEQ IDNO.24; the tag sequence is selected from SEQID NO.1 to SEQID NO.18; and the microballons are respectively coated by a specific anti-tag sequence and have codes in different colors. The invention also discloses the SNP detection liquid phase chip for apoE and KIF6 genes and the chromosome 9p21 section. The occlusion rate of the detection method and the sequencing method which are provided in the invention is as high as 100 percent. The prepared SNP detection liquid phase chip for the apoE and KIF6 genes and the chromosome 9p21 section has favorable single-noise ratio.

The invention discloses a specificity primer and a liquid phase chip for SNP detection of CYP4F2 gene. The liquid phase chip comprises an ASPE primer formed by the specificity primer aiming at a target gene SNP locus of the tag sequence on the 5' end and 3' end, wherein the specificity primer is SEQ ID NO.13 and SEQ ID NO.14 aiming at the G1297A SNP locus and/or SEQ ID NO.15 and SEQ ID NO.16 aiming at the T34G SNP locus, a microsphere and an amplification primer. The invention also provides a liquid phase chip for SNP detection of CYP4F2 and EPHX1 genes, which comprises the corresponding compositions of the liquid phase chip for the SNP detection of the CYP4F2 and EPHX1 genes, an ASPE primer pair aiming at the G357A, G19512990A, T337C and/or A416G SNP locus of the EPHX1 gene, microsphere and amplification primer. The detection liquid phase chip has an excellent signal-noise ratio, can avoid cross reaction and can realize the parallel detection of a plurality of SNP loca.

The invention discloses BRAP and PSMA6 gene SNP detection specific primers and a liquid phase chip. The liquid phase chip comprises an ASPE primer which is composed of a tag sequence at a 5' terminal and specific primers mutated for a target gene at a 3' terminal, wherein the specific primers respectively comprise a SEQ ID NO.7 and a SEQ ID NO.8 for a BRAP gene A96G SNP locus, a SEQ ID NO.9 and a SEQ ID NO.10 for a BRAP gene A80G SNP locus, and/or a SEQ ID NO.11 and a SEQ ID NO.12 for a PSMA6 gene C157G SNP locus; microspheres coated by an anti-tag sequence; and amplification primers. The coincidence rate between detection results of the BRAP and PSMA6 gene SNP detection liquid phase chip provided by the invention and results of a sequencing method is up to 100%.

The invention discloses a specific primmer and a liquid phase chip for solute carrier organic anion transporter 1B1 (SLCO1B1) gene signal nucleotide polymorphism (SNP) detection. The liquid phase chip mainly comprises allele specific primer extension (ASPE) primer pairs, microspheres and amplifiers, wherein each ASPE primer consists of a tag sequence at a 5' end and specific primers at a 3' end aiming at an SNP locus; the specific primers comprise SEQ ID NO.11 and SEQ ID NO.12 aiming at T521C SNP locus, SEQ ID NO.13 and SEQ ID NO.14 aiming at T89595C SNP locus, SEQ ID NO.15 and SEQ ID NO.16 aiming at A388G SNP locus, SEQ ID NO.17 and SEQ ID NO.18 aiming at C463A SNP locus, and/or SEQ ID NO.19 and SEQ ID NO.20 aiming at A1929C SNP locus; and the tag sequence is selected from SEQ ID NO.1 to SEQ ID NO.10. The coincidence rate of the liquid phase chip provided by the invention and the detection result of a sequencing method reaches 100 percent. And the prepared liquid phase chip for the SLCO1B1 gene SNP detection has high signal-to-noise ratio.

The invention discloses specific primers and a liquid phase chip for detecting the polymorphism of a CDKAL1 gene. The liquid phase chip mainly comprises specific primers consisting of a tag sequence at a 5' end and specific primer sequences of polymorphic sites of a target gene, microspheres and amplification primers, wherein the specific primer sequences are one or more pairs from SEQ ID No.9 and SEQ ID No.10 for A107C, SEQ ID No.11 and SEQ ID No.12 for G323C, SEQ ID No.13 and SEQ ID No.14 for A210G and SEQ ID No.15 and SEQ ID No.16 for A75G; the tag sequence may be a sequence from SEQ ID No.1 to SEQ ID No.8. The prepared liquid phase chip for detecting the polymorphism of the CDKAL1 gene has a very high signal-to-noise ratio, and the cross reaction of a designed probe and an anti-tae sequence is prevented basically.

The invention discloses a XRCC2 gene mutation detection liquid chip, and specific primers. The XRCC2 gene mutation detection liquid chip mainly comprises: ASPE primers, wherein each ASPE primer is composed of 5'-terminal tag sequence, and 3'-terminal specific primer sequence targeting target gene mutation sites, and the specific primer sequence comprises SEQ ID No.9 and SEQ ID No.10 targeting G87A site, SEQ ID No.11 and SEQ ID No.12 targeting G158C site, SEQ ID No.13 and SEQ ID No.14 targeting A82G site, and/or SEQ ID No.15 and SEQ ID No.16 targeting A155C site; microballoons coated with anti-tag sequences; and amplification primers. Self-agreement ratio of detection results of the liquid chip with detection results of sequencing is as high as 100%; and parallel detection of wild types and mutant types of a plurality of mutation sites is realized.

The invention discloses a liquid chip and specific primer for detecting SNP of a GPIIIa gene. The liquid chip mainly comprises a wild type ASPE primer and a mutant type ASPE primer which are respectively designed aiming at the SNP loci of the GPIIIa gene, microspheres which are respectively coated with specific anti-tag sequences and have different colors of codes and an amplification primer for amplifying the GPIIIa gene target sequence containing the T196C SNP loci, wherein each ASPE primer is formed by the tag sequence at the 5, terminal and the specific primer, aiming at the SNP loci of the target gene, at the 3, terminal; and the anti-tag sequences are selected from SEQ ID NO.17 to SEQ ID NO.24 and correspondingly complement and are paired with the tag sequences selected from each ASPE primer. The invention also provides a liquid chip and specific primer for detecting SNP of COX-1 and GPIIIa genes. Besides the corresponding composition of the GPIIIa gene, the liquid chip also comprises ASPE primer pair aiming at the SNP loci of the COX-1 gene, microspheres coated with specific anti-tag sequences and an amplification primer. The liquid chip has very good signal to noise ratio,can avoid cross reaction and can realize parallel detection of a plurality of SNP loci.

The present invention discloses a detection liquid phase chip and specific primers for FYCO1 gene mutation. The liquid phase chip mainly comprises: ASPE primers comprising a 5' terminal tag sequence and a 3' terminal target gene mutational site-targeted specific primer sequence, wherein the specific primer sequence comprises A92G site-targeted SEQ ID NO.1, A92G site-targeted SEQ ID NO.2, and/or C57T site-targeted SEQ ID NO.3 and C57T site-targeted SEQ ID NO.4; anti-tag sequence coated microspheres; and amplification primers. According to the present invention, coincidence frequency of the detection results of the detection liquid phase chip and the sequencing method is up to 100%, and single and parallel detection on the wild-type with multiple mutational sites and the mutant-type with multiple mutational sites can be achieved.

The invention discloses specific primers and a liquid-phase chip for SNP (Single Nucleotide Polymorphism) detection of MTHFR and FGF5 genes. The liquid-phase chip comprises an ASPE primer, anti-tag sequence coated microspheres, and an amplification primer, wherein the ASPF primer is composed of a 5'-terminal tag sequence and 3'-terminal specific primers for target gene mutation, and the specific primers are respectively SEQ ID NO. 9 and SEQ ID NO. 10 specific for a G121A SNP site, SEQ ID NO. 11 and SEQ ID NO. 12 specific for an A105G SNP site, SEQ ID NO. 13 and SEQ ID NO. 14 specific for a G111A SNP site, and/or SEQ ID NO. 15 and SEQ ID NO. 16 specific for a T196A SNP site of the FGF5 gene. According to the invention, the coincidence rate between the detection result of the liquid-phase chip for SNP detection of the MTHFR and FGF5 gene and the detection result of a sequencing method is up to 100%.

The invention discloses specific primers and a liquid phase chip for SNP (Single Nucleotide Polymorphism) detection of a STK39 (Serine/Threonine Kinase) gene. The liquid phase chip mainly comprises an ASPE (Allele Specific Primer Extension) primer composed of a tag sequence at a 5' side and specific primers aiming at target gene mutation at a 3' side. The specific primers are respectively: SEQ ID NO.7 and SEQ ID NO.8 aiming at the T107C SNP site, SEQ ID NO.9 and SEQ ID NO.10 aiming at the G232A SNP site, and/or SEQ ID NO.11 and SEQ ID NO.12 aiming at the A190C SNP site; a microsphere coated with an anti-tag sequence; and an amplification primer. The coincidence rate of the detection result of the liquid phase chip for SNP detection of the STK39 gene provided by the invention and a sequencing method is as high as 100%. The prepared liquid phase chip has an excellent signal-noise ratio and can realize parallel detection of multiple polymorphism sites.

The invention discloses CYP2B6 gene detection specific primers and a liquid chip. The liquid chip mainly comprises: various ASPE primers composed of 5' terminal tag sequence and 3' terminal specific primer sequences aiming at target gene mutation site, microspheres coated with anti-tag sequence, and amplification primers. The specific primer sequences comprise: SEQ ID NO.13 and SEQ ID NO.14 aiming at C123T site; SEQ ID NO.15 and SEQ ID NO.16 aiming at A101G site; SEQ ID NO.17 and SEQ ID NO.18 aiming at G118T site; SEQ ID NO.19 and SEQ ID NO.20 aiming at A77G site; SEQ ID NO.21 and SEQ IDNO.22 aiming at T139C site; and/or SEQ ID NO.23 and SEQ ID NO.24 aiming at C288T site. The matching rate of the detection result obtained by using the liquid chip provided by the invention and a result obtained by using a sequencing method is 100%. Therefore, wild-type and mutant-type parallel detections of a plurality of mutation sites are realized.