CYP2D6 gene mutation detection liquid-phase chip and detection method

Abstract

The invention provides a CYP2D6 gene mutation detection liquid-phase chip which comprises ASPE (Allele Specific Primer Extension) primers aiming at CYP2D6 C2850T and CYP2D6Deletion mutational sites, three microballoons respectively enveloped with a specific anti-tag sequence and amplification primers aiming at the CYP2D6C2850T and the CYP2D6 Deletion mutational sites. The CYP2D6 gene mutation detection liquid-phase chip can simultaneously detect aiming at the CYP2D6 C2850T and the CYP2D Deletion mutational sites and has excellent signal to noise ratio. The coincidence ratio with a sequencing method of the CYP2D6 gene mutation detection liquid-phase chip reaches up to 100 percent, and the CYP2D6 gene mutation detection liquid-phase chip has higher specificity and precision compared with intra-class correlation products.

Description

technical field

[0001] The invention belongs to the field of molecular biology, relates to medicine and biotechnology, and in particular relates to a CYP2D6 gene mutation detection liquid chip and a detection method. Background technique

[0002] Tamoxifen (TAM, tamoxifen, also known as tamoxifen) is a selective estrogen receptor modulator whose structure is similar to estrogen. After entering breast cancer cells, estrogen can bind to estrogen receptor (ER, estrogen receptor), promote the synthesis of DNA and m-RNA of tumor cells, and stimulate the growth of tumor cells. When TAM enters the cell, it can compete with estrogen to bind to the estrogen receptor, preventing the function of estrogen, thereby inhibiting the proliferation of breast cancer cells. TAM treatment belongs to one of the endocrine treatment of breast cancer, and another commonly used endocrine therapy drug is aromatase inhibitors (aromatase inhibitors). However, aromatase inhibitors are only suitable for...

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