8 results about "Gene duplication" patented technology

The present invention relates to methods, systems, and compositions for counting nucleic acid molecules. The present invention relates to a method for detecting changes in the number of molecules without digital sequencing, in particular for example due to gene duplication or normal euploid complements of chromosomes, compositions and methods, systems, and kits for detecting and quantifying changes in gene dose, such as changes in trisomes of one or more chromosomes typically found in diploid pairs.

Compositions, methods, systems and kits for detecting and quantifying changes in molecular number, specifically changes in gene dosage, such as due to gene duplication or changes in the normally euploid complement of chromosomes, such as trisomy of one or more chromosomes normally found in a diploid pair, in the absence of digital sequencing.

This invention belongs to the fields of bioinformatics and molecular biology, specifically relating to a method for identifying gene duplication and functional differentiation in Rosaceae genera. By analyzing MAPK family proteins in rose and Arabidopsis thaliana, the invention identifies HT1, ANP3, Raf42, and Raf24 proteins that are duplicated and functionally differentiated in rose. Further application of bioinformatics and molecular biology techniques to Rosaceae plants reveals that only ANP3 exhibits duplication and functional differentiation in all Rosaceae plants. This invention provides a molecular-level reference for exploring the function of the MAPK family and the functional differentiation among ANP3-like genes in Rosaceae, laying the foundation for future research and identification of other duplicated and functionally differentiated genes in Rosaceae.

To provide a method for detecting and quantifying variations in numbers of molecules, particularly variations in gene dosage, due to gene duplication or to trisomy of one or more chromosomes that are normally found in diploid pairs, without digital sequencing.SOLUTION: The present invention provides a method comprising: (a) forming at least one complex comprising an oligonucleotide primer hybridized to a circularized nucleic acid, wherein the primer is bound to a solid support surface; and (b) exposing the at least one complex to a reaction mixture comprising DNA polymerase, one or more deoxynucleoside triphosphates (dNTPs), and at least 12% polyethylene glycol (PEG), wherein the PEG has an average molecular weight between 400 and 800, under conditions in which the primer in the complex is extended on the circularized nucleic acid by the DNA polymerase to form an extended primer.SELECTED DRAWING: Figure 1

Compositions and methods, systems, and kits for detecting and quantifying variations in numbers of molecules, particularly variations in gene dosage, e.g., due to gene duplication, or to variations from the normal euploid complement of chromosomes, e.g., trisomy of one or more chromosomes that are normally found in diploid pairs, without digital sequencing.

The present invention discloses a high-throughput gene sequencing method based on the whole genome, which relates to the technical field of gene detection and includes: establishing a whole genome recognition library; forming a set of test gene replication sequences from at least one gene replication sequence to be tested; forming at least one set of test cleavage genes; obtaining one of the test gene replication sequences in the set of test gene replication sequences; forming a set of test gene replication fragments from at least one test gene replication fragment; obtaining at least one set of test gene replication fragments; obtaining the categories of test gene replication fragments; obtaining the sequencing results of the test gene replication fragments; and obtaining the sequencing results of the test gene replication sequences. By using fluorescence-labeled cleavage genes to perform split detection on the test gene replication sequences, it avoids sequencing errors caused by contamination of a single slice, can greatly reduce the complexity of merging, and thus effectively shortens the detection time.

An engineered yeast strain capable of efficient fermentation of xylose to ethanol, and methods of making and using the strain, are provided.