The invention discloses a compound bergamot oil nano-emulsion composition, which is characterized in that: the granularity of the nano-emulsion is 1nm to 100nm, and comprises the following substances by weight mass: 23 to 35 percent of surface active agent, 0.3 to 8 percent of cosurfacant, 1.5 to 10 percent of bergamot oil, 0.5 to 5.5 percent of patchouli, 0.4 to 4.5 percent of tea tree oil, 0.3 to 2.2 percent of pennyroyal and distilled water, wherein the sum of the mass percentage of the ingredients is 100 percent. The compound bergamot oil nano-emulsion composition is broad-spectrum antimicrobic, has good treatment effect on eczema, psoriasis, herpes, dermatitis, blain, wound, acne, urinary infection and the like, the permeability is strong, the stability is good, content of active ingredients is high, transdermal absorption effect is remarkable, action time is durable, the preparation method is simple and is easy to popularize and use, and the medicine has vast application prospect on the medical market.