Micromolecular substance for improving sensitivity of bacteria to antibiotics

A technique for antibiotics, susceptibility, used in the field of medicine

Active Publication Date: 2013-03-20
GUANGDONG LITAI PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on improving the susceptibility of persistent bacteria to antibiotics with other metabolites and other antibiotics, and there is no research report on the use of small molecule metabolites to improve bacterial susceptibility to antibiotics

Method used

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  • Micromolecular substance for improving sensitivity of bacteria to antibiotics
  • Micromolecular substance for improving sensitivity of bacteria to antibiotics
  • Micromolecular substance for improving sensitivity of bacteria to antibiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Screening of antibiotic resistant strains of Escherichia coli K12 BW25113

[0038] Escherichia coli ( Escherichia coli , E. coli )) is the most common type of bacteria in the intestinal tract of humans and animals, mainly in the large intestine, accounting for about 1% of intestinal bacteria. Detect the starting bacteria of Escherichia coli ( Escherichia coli K12 BW25113) minimum inhibitory concentration (MIC) for 10 antibiotics. Will 10 5 The starting bacteria of colony forming unit / ml were cultured in 10 kinds of LB liquid medium containing 1 / 2 of the minimum inhibitory concentration of antibiotics at 37°C and successively passaged 10 times, and the minimum inhibitory concentration of the obtained single clone was determined. The results showed that the minimum inhibitory concentrations of the 10 selected bacteria against the 10 antibiotics were 64 times the minimum inhibitory concentrations of the respective starting bacteria (see Table 1), indicating that 10 types...

Embodiment 2

[0043] Glutamine is a key biological target substance of ampicillin-resistant Escherichia coli

[0044] 1. Preparation of GC-MS samples of ampicillin-resistant Escherichia coli

[0045] 1) Preparation of bacterial samples: pick the starting bacteria of Escherichia coli from the solid LB plate ( Escherichia coli K12 BW25113) and a single colony of ampicillin-resistant bacteria (see Example 1 for the specific method of culturing resistant bacteria), inoculate in 5 ml of LB medium, culture at 37°C and 200 rpm for 16 hours; press 1:100 (v / v) Inoculated in 100 ml of LB liquid medium, and cultivated at 37°C to an OD600 value of 1.0.

[0046] 2) Preparation of GC-MS samples: Take 10 ml of bacterial solution and quickly add 5 ml of frozen methanol to quench the sample to stop the metabolic process of cells. Collect the bacteria by centrifugation at 4°C and 8000rpm for 3 minutes, remove the water as much as possible, and store the bacteria in 2 ml of cold methanol (Sigma).

[0047] 3) Ext...

Embodiment 3

[0054] Glutamine down-regulation is widespread in various antibiotic-resistant bacteria of Escherichia coli

[0055] 1. Preparation of GC-MS samples of various antibiotic-resistant Escherichia coli bacteria

[0056] Cultivation of Escherichia coli starting bacteria ( Escherichia coli K12 BW25113) and its 10 resistant E. coli strains: GEN-R, AMP-R, BLFX-R, KAN-R, NA-R, CAZ-R, ER-R, CAP-R, SM-R and TET -R, refer to Example 2.1 for the sample preparation process

[0057] 2. The down-regulation of glutamine in various antibiotic-resistant bacteria of Escherichia coli is a common phenomenon

[0058] The prepared 10 kinds of drug-resistant bacteria samples were loaded on GC-MS to analyze metabolites. According to the retention time of glutamine in the GC-MS total ion flow chart, the peak area data of the glutamine peak and the internal standard peak of each drug-resistant bacteria are obtained, and the relative peak area (ratio of the internal standard peak) is used to represent the m...

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Abstract

The invention belongs to the technical field of medicines and particularly relates to a new function of micromolecular metabolin, namely glutamine. The micromolecule can improve the sensitivity of bacteria including drug-resistant bacteria to antibiotics so as to solve the problem of drug resistance of bacteria. The micromolecule provided by the invention has better effect and higher safety and operability compared with antibiotic which is applied to the medicament for resisting drug resistance of bacteria at present.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a small molecular substance-glutamine which can improve the susceptibility of bacteria to antibiotics. Background technique [0002] The invention and application of antibiotics play an essential role in the protection of human health and life. However, due to the abuse of antibiotics in recent years, it has become a key factor that threatens human health, poultry, livestock and aquaculture, and the ecological environment today. According to statistics from the Ministry of Health, 80,000 people in my country die directly or indirectly from the abuse of antibiotics every year; the damage caused by the abuse of antibiotics and the emergence of drug-resistant bacteria are even more immeasurable. The resistance of some bacteria clinically isolated in my country to certain drugs has ranked first in the world. The drug resistance rate of quinolone antibiotics in my countr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/198A61K45/00A61K31/43A61K31/546A61K31/4709A61K31/4375A61K31/7036A61K31/7048A61K31/65A61K31/496A61K31/7056A61P31/04
CPCY02A50/30
Inventor 彭宣宪李惠赵贤亮
Owner GUANGDONG LITAI PHARM CO LTD
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