371 results about "Resistant organism" patented technology

The invention discloses a polypeptide drug for resisting gram-negative bacteria and application, and belongs to the technical field of biological medicine. The MIC of the antibacterial peptide obtained through screening on escherichia coli, salmonella typhimurium, pseudomonas aeruginosa, klebsiella pneumoniae, shigella sonnei and stenotrophomonas maltophilia is 16 [mu] g / mL, 2 [mu] g / mL, 16 [mu] g / mL, 4 [mu] g / mL, 2 [mu] g / mL and 4 [mu] g / mL respectively, and the antibacterial peptide has broad-spectrum antibacterial performance. The hemolytic activity and toxicity of the compound are lower than therapeutic concentration threshold values, and the compound can be used for preparing drugs or medical products for treating multi-drug-resistant bacterium infection.

The invention relates to the technical field of environmental functional materials and water treatment, and discloses a preparation method and application of a persulfate activator for efficiently removing drug-resistant bacteria and drug-resistant genes in water. Waste biomass and waste plastics are used as raw materials, solid waste resource utilization is achieved through synergistic pyrolysis, compared with a traditional catalyst, the preparation cost can be reduced by more than 40%, and remarkable economic and environmental benefits are achieved. The catalyst has the following advantages: 1) the limitation of a traditional single-active-site catalyst is broken through through the triple synergistic effect of transition metal oxide variable valence activation, nonmetal doped carbon electron conduction and an oxygen vacancy non-free radical path; 2) physical interception of ARB and enrichment of ARGs can be realized at the same time by utilizing a multi-stage pore channel structure; and (3) activating persulfate to generate free radicals (SO4 <->. / . OH) and non-free radicals (1O2 / electron transfer) to generate a double-path synergistic effect, thereby realizing efficient removal of ARB / ARGs in water.

The invention provides a cyclic peptide as well as a preparation method and application thereof. The cyclic peptide has an amino acid sequence # imgabs0 # represented by formula (1) (wherein X is a Lys residue modified with a fatty acid molecule, and a line connecting Arg and Lys represents an amide bond. The cyclic peptide prepared by the invention has wide antifungal activity and antibacterial activity on drug-resistant bacteria; meanwhile, the biotoxicity and the protease degradation resistance are high, and the in-vivo antibacterial effect of the cyclopeptide can be prolonged. The cyclic peptide can be applied to preparation of anti-bacterial and anti-fungal infection drugs, and can be used as an excellent substitute drug or an auxiliary drug of existing antibiotics. The method for preparing the cyclic peptide is simple, raw materials are easy to obtain, and industrial production can be achieved.

The invention relates to the technical field of high-throughput targeted sequencing, in particular to a primer group for detecting important drug-resistant genes and virulence genes of multi-drug-resistant pathogenic bacteria and application of the primer group. Specifically, the kit comprises detection primers for multiple drug-resistant bacteria such as third-generation cephalosporin drug-resistant enterobacteriaceae bacteria, carbapenem drug-resistant enterobacteriaceae bacteria, carbapenem drug-resistant acinetobacter baumannii, rifampicin drug-resistant mycobacterium tuberculosis and the like. The method has the technical advantages of rapidness, high efficiency, strong targeting property, strong specificity and the like, is suitable for epidemic and difference analysis of various important drug-resistant genes and virulence genes carried by metagenome samples of various environments or disease materials and the like, evaluates the risk of related environments on the basis, and can further guide prevention and clinical treatment medication.

The invention discloses a spatio-temporal trajectory fusion-based closed-loop monitoring method and system for drug-resistant bacteria in a hospital. The method comprises the following steps: collecting and processing strain drug sensitive test results and patient clinical index data in a hospital information system, and screening out key features through an improved genetic algorithm; wherein the clinical index data of the patient comprises original spatio-temporal trajectory data and nursing operation records; analyzing spatio-temporal trajectory data of the patient according to the key features, combining with drug sensitivity spectrum consistency, and constructing a propagation network model by using a graph convolutional network to identify infection source nodes and corresponding topological influence; and generating an early warning signal based on the topological influence of the infection source node, and continuously optimizing prevention and control measures by dynamically adjusting model parameters and a real-time feedback mechanism. By implementing the method provided by the invention, accurate analysis and real-time prevention and control of a drug-resistant bacterium transmission link can be realized.

The invention relates to the technical field of biology, in particular to glycopeptide antibiotic and application thereof. According to the invention, a biosynthetic gene cluster for synthesizing a brand new glycopeptide antibiotic skeleton is excavated, and brand new glycopeptide antibiotics Varsomycin A, B and C with novel chemical structures are obtained through heterologous expression; the traditional Chinese medicine composition has a remarkable antibacterial effect on clinically drug-resistant strains. Important fermentation strains and precursor compound molecules are provided for research and development of new drugs of glycopeptide antibiotics, and important application prospects and economic values are achieved.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide Mh-GC21 as well as a precursor and application thereof. The amino acid sequence of the antibacterial peptide Mh-GC21 provided by the invention is as shown in SEQ ID NO.1, and two cysteines are oxidized to form a pair of intermolecular disulfide bonds; the C tail end of the antibacterial peptide Mh-GC21 is subjected to amidation modification. The antibacterial peptide Mh-GC21 provided by the invention is from microhyla microcarpa, has broad-spectrum antibacterial activity, has relatively good antibacterial and bactericidal effects on standard strains of acinetobacter baumannii, escherichia coli and staphylococcus aureus and clinically sourced drug-resistant strains, and can be used for targeted destruction of cell membranes of bacteria, removal of biological membranes and inhibition of formation of the biological membranes. Moreover, the antibacterial peptide Mh-GC21 has the advantages of good stability, no hemolytic activity and cytotoxicity, and difficulty in inducing strains to generate drug resistance.

The invention discloses a hybrid Ag / MXene / Fe-Co MOF surface enhanced Raman scattering sensor and an application of the hybrid Ag / MXene / Fe-Co MOF surface enhanced Raman scattering sensor in rapid detection of bacteria. According to the sensor, silver nanoparticles are prepared through a sodium citrate reduction method, a two-dimensional material MXene and a bimetal organic framework are combined, and a surface enhanced Raman scattering spectrum detection platform with high sensitivity, stability and anti-interference performance is constructed. The conductive network of MXene and the local surface plasmon resonance effect of silver nanoparticles synergistically enhance an electromagnetic field, and the porous structure of Fe-Co MOF enriches target molecules and forms dense hot spots, so that the Raman signal intensity is remarkably improved. Experiments show that the detection limit of the sensor to 4-mercaptobenzoic acid is as low as 10 <-12 > M, and the signal stability can last for 7 days; the detection limit on common pathogenic bacteria such as escherichia coli and staphylococcus aureus reaches 10 CFU / mL, and the linear correlation coefficient is superior to 0.96. The sensor has hydrophobicity and biocompatibility, can efficiently capture bacteria through capillary force, is suitable for trace detection of complex samples, and provides a new strategy for rapid diagnosis of clinical drug-resistant bacteria.

The invention discloses an application of kaempferol in preparation of a medicine for treating or preventing PEDV (Porcine Epidemic Diarrhea Virus) infection. The kaempferol can obviously inhibit the replication of the PEDV in Vero and LLC-PK1 cells through 3C-like protease targeting the PEDV, so that the kaempferol can be used for preparing the medicine for preventing and treating the PEDV. Besides, kaempferol, as a natural substance separated from mulberries, can be used as a feed additive for a long time so as to achieve the purpose of preventing epidemic diseases, and does not cause generation of drug-resistant strains and other toxic and side effects after being used for a long time; the defects of immune failure possibly caused by vaccination, virus variation caused by use of antiviral drugs and the like are overcome.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an LL-37 derived antibacterial peptide and application thereof. According to the invention, 17-29aa of the LL-37 antibacterial peptide is used as a core fragment and is connected with an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2 through an amido bond, the antibacterial activity of the obtained antibacterial peptide is obviously higher than that of the LL-37 antibacterial peptide, and the antibacterial peptide shows an obvious bactericidal effect on clinical drug-resistant bacteria such as acinetobacter baumannii, pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, klebsiella pneumoniae and the like; meanwhile, the cytotoxicity and hemolytic activity are weak, drug resistance is not prone to being generated, bacteria can be rapidly killed in vivo through multiple mechanisms, no obvious cytotoxicity exists, the bacterium load in tissue is remarkably reduced, and the lifetime of a bacterium-infected mouse is prolonged.

The invention discloses a preparation method of photo-thermal-MMP dual-response matrix hydrogel and application of the photo-thermal-MMP dual-response matrix hydrogel in diabetic wound repair, and belongs to the technical field of biological medicine. According to the present invention, the Cur (at) ZIF-8 (at) MMPDA / dAMG composite hydrogel is composed of an amnion source photo-crosslinked matrix hydrogel, curcumin-loaded ZIF-8 nanoparticles and MMP2 peptide modified polydopamine, and the Cur (at) ZIF-8 (at) MMPDA / dAMG composite hydrogel is obtained; the ZIF-8 nanoparticles decline in an acid environment to release zinc ions, destroy bacterial cell membranes, enhance the permeability of a biological membrane and cooperatively kill drug-resistant bacteria in combination with a photothermal effect, Cur is loaded through ZIF-8 to form Cur-coated ZIF-8, then a Cur-coated ZIF-8-coated MMPDA / dAMG double-drug-loading system is constructed through an MMPDA medium, and a photothermal antibacterial, anti-inflammatory and regeneration-promoting multi-mode therapy is formed for a wound surface in the acid environment. Therefore, wound healing is accelerated.

The invention provides engineering lyase Art-15 with broad-spectrum antibacterial activity, antibacterial peptide NZ2114 and bacteriophage lyase PlySs2 are subjected to functional fusion through an optimally designed flexible linker, and an efficient bactericidal effect on Staphylococcus aureus (Staphylococcus aureus) and Streptococcus spp.) is achieved. The engineering lyase Art-15 has the advantages that the engineering lyase Art-15 has broad-spectrum antibacterial activity, the antibacterial peptide NZ2114 and the bacteriophage lyase PlySs2 are subjected to functional fusion through the optimally designed flexible linker, and the engineering lyase Art-15 has broad-spectrum antibacterial activity; the lyase Art-15 overcomes the defects of narrow host spectrum and insufficient splitting activity of the lyase through a synergistic destruction mechanism of targeting bacterial cell wall peptidoglycan. Experiments show that the minimal inhibitory concentration (MIC) of the lyase Art-15 on staphylococcus aureus in vitro is lower than 2 mu g / mL, the minimal inhibitory concentration of the lyase Art-15 on streptococcus agalactiae is lower than 1 mu g / mL, the minimal inhibitory concentration of the lyase Art-15 on streptococcus dysgalactiae is lower than 2 mu g / mL, and the sterilization rate of the lyase Art-15 can reach 97% or above within 30 minutes by 2 times of MIC. The lyase Art-15 disclosed by the invention has important application value in the fields of drug-resistant bacterium infection treatment, medical instrument disinfection and livestock breeding.

The invention discloses a composite antibacterial preparation containing antibacterial peptide and traditional Chinese medicines as well as a preparation method and application of the composite antibacterial preparation, and belongs to the technical field of biological medicines. The antibacterial peptide provided by the invention is a transmembrane protein derived from bacteriophage, and the core function of the antibacterial peptide is that bacterial cell lysis is promoted by destroying a connection structure of a peptidoglycan layer and lipoprotein of a bacterial cell wall, so that an antibacterial effect is achieved. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO: 3, the antibacterial peptide is combined with traditional Chinese medicines to form a composite antibacterial preparation, and experiments prove that the composite antibacterial preparation has a relatively good antibacterial effect on gram-positive bacteria and gram-negative bacteria and can be applied to the medical field, such as surgical instrument disinfection and drug-resistant bacterial infection treatment; the field of daily chemicals, such as antibacterial hand sanitizers and wound dressings; in the field of agriculture, for example, animal feed additives for preventing and treating intestinal infection have a good market application prospect.

The invention discloses bioactive gel for accelerating wound healing. The gel is prepared from the following raw materials in percentage by weight: 0.8%-1% of bomer 980, 5%-5.5% of glycerol, 15%-20% of white vaseline, 51.7%-55% of phosphate buffer, 1.8%-2% of nano royal jelly acid, 2%-3% of recombinant III type human collagen, 0.4%-0.5% of sodium hyaluronate, 1%-3% of sodium alginate, 20%-25% of polyethylene glycol-400, 1.5%-3% of dopamine-methacrylamide copolymer, 0.5%-0.8% of nano silver and 0.3%-0.5% of phenoxyethanol. The nano royal jelly acid and the nano silver have a synergistic antibacterial effect, so that the inhibition effect on drug-resistant bacteria such as MRSA and pseudomonas aeruginosa is remarkably improved, and meanwhile, the wound epithelization can be accelerated and scar formation can be reduced through the dual effects of the recombinant III type collagen and the acetylated sodium hyaluronate. The defects that a traditional dressing is narrow in antibacterial spectrum, single in repairing effect, poor in environmental adaptability and the like are overcome, and the dressing is particularly suitable for complicated wounds such as diabetic foot ulcer and second-degree burn.

The invention discloses a broad-spectrum antibacterial peptide as well as a screening method and application thereof, and belongs to the technical field of antibacterial peptides, a bioinformatics method is mainly adopted for searching a new antibacterial peptide, then amino acid sequence optimization is carried out, and finally the antibacterial peptide with a better antibacterial effect is obtained. Sterilization activity evaluation is carried out by using various known strains with drug resistance, and the result shows that the antibacterial peptide provided by the invention has broad-spectrum antibacterial activity on drug-resistant bacteria, more comprehensive antibacterial effect and wider indications; the compound has the advantages of no hemolysis, low cytotoxicity, efficient in-vivo antibacterial activity and high stability, and has application safety; the compound has a wide application prospect of being developed into a human body antibacterial drug.

The invention discloses a multi-drug-resistant bacterium infection risk assessment method, system and device and a storage medium, and belongs to the technical field of medical data analysis, and the method comprises the steps: collecting a clinical related data set of an ICU patient, and carrying out the preprocessing of the clinical related data set to obtain a to-be-assessed data set; performing feature extraction on the to-be-evaluated data set in combination with a long short-term memory network and a time sequence attention mechanism to obtain a high-risk feature set; inputting the high-risk feature set into a pre-constructed dynamic scoring model, and calculating a corresponding risk score through the dynamic scoring model; and grading the risk score based on a preset grading rule to obtain a current risk level, and outputting a corresponding intervention strategy through a preset intervention decision mechanism based on the current risk level. According to the scheme, the high-risk feature set is extracted by combining the long-short-term memory network with the time sequence attention mechanism, then the risk score is calculated through the dynamic scoring model, the risk level is determined according to the preset grading rule, the intervention strategy is output, and the accuracy of MDRO infection assessment is improved.

The invention relates to the field of drug-resistant bacterium infection, and discloses a g-C3N4 / Bi2Se3 nano heterojunction and a preparation method thereof.The preparation method comprises the steps that firstly, melamine powder is heated and calcined at the constant temperature, after a tubular furnace is naturally cooled, spark plasma sintering is conducted, nitrogen vacancies are introduced at the same time, and a blocky high-defect g-C3N4 material is obtained; grinding into powder, adding deionized water for ultrasonic treatment to obtain a first suspension, performing centrifugal treatment, freeze-drying the supernatant, and adding into an ethylene glycol solvent to obtain a second suspension; eG, polyvinylpyrrolidone, Bi (NO3) 3.5 H2O and Na2SeO3 are mixed and stirred, and the second turbid liquid is added; the preparation method comprises the following steps: adding Bi2Se3 into a reaction kettle, heating while stirring, adding a hydroxylamine EG solution, cooling to room temperature after reaction, centrifuging, washing, and carrying out vacuum drying on a precipitate to obtain a g-C3N4 / Bi2Se3 nano heterojunction; the problems that in the prior art, the catalytic efficiency is low, a collaborative mechanism does not achieve structure-function integrated design, the performance of a key functional material is limited, and the structural stability and biological suitability of the material are insufficient are solved.

The invention discloses an early warning method and system for outbreak of drug-resistant bacteria in a hospital based on dynamic aggregation degree evaluation. The method comprises the following steps: acquiring hospitalization information of a patient, microbiological inspection data and spatial layout information of an inpatient area to obtain initial data; judging whether a new case of drug-resistant bacteria is detected on the current day; if not, gradually reducing the historical aggregation degree by using an exponential decay formula until the historical aggregation degree returns to zero; if yes, calculating a newly added detection rate, a space aggregation degree and a time aggregation degree, and calculating a total aggregation degree of the day; dividing an inpatient area; dynamically generating a threshold value for the high-frequency inpatient area by adopting a quartile method to carry out abnormal value judgment, and identifying an abnormal aggregation degree for the low-frequency inpatient area by using a fixed absolute value threshold mechanism to obtain an early warning level; and early warning information of different levels is pushed according to the early warning level, and a prevention and control suggestion plan is attached. By implementing the method provided by the invention, the accuracy and efficiency of early warning can be remarkably improved, so that patients and medical personnel are better protected from the threat of drug-resistant bacterium infection.

The invention discloses a multi-mode synergistic antibacterial nano material as well as a preparation method and application thereof. The multi-mode synergistic antibacterial nano material comprises a two-dimensional silylene nanosheet and a copper nanocluster loaded on the surface of the two-dimensional silylene nanosheet. According to the Si-coated Cu NSs composite material disclosed by the invention, copper nanoclusters (CuNCs) are loaded on the surface of a two-dimensional silylene nanosheet, the catalytic activity of copper is enhanced by utilizing a p-d orbital hybridization effect, and meanwhile, photo-thermal therapy (PTT) and chemical kinetic therapy (CDT) are combined to synergistically remove drug-resistant bacteria and biological membranes, so that the limitation of traditional two-dimensional silylene in antibacterial application is overcome.

The invention discloses a preparation method and antibacterial application of a naphthothiadiazolyl photosensitizer, and belongs to the technical field of biomedical antibacterial materials. The naphthothiadiazole-based photosensitizer provided by the invention overcomes the problem that the existing commercial photosensitizer gathers and quenches the active oxygen efficiency, and meanwhile, the antibacterial photosensitive polymer prepared based on the novel naphthothiadiazole-based photosensitizer has the unique advantages that the structure is stable, photosensitizer micromolecules are not easy to leak and the antibacterial effect is long-acting; the construction of the antibacterial polymer coating on the surface of the medical instrument in the future has important clinical application potential. The naphthothiadiazolyl photosensitizer provided by the invention has good photodynamic killing performance on drug-resistant bacteria MRSA (Methicillin Resistant Staphylococcus Aureus).

The invention discloses a pathogenic bacterium infection prevention and treatment krill NC-K143, a bacteriostatic agent and application of the pathogenic bacterium infection prevention and treatment krill NC-K143 and the bacteriostatic agent. According to the present invention, the classification name of the Leptospora sp. NC-K143 is Kitasatospora sp.NC-K143, the strain has been preserved in the China Center for Type Culture Collection on March 27, 2025, and the preservation number is CCTCC NO: M 2025616. The invention further provides the application of the Leptospora sp. NC-K143, and the Leptospora sp. NC-K143 and the application of the Leptospora sp. NC-K143, according to the present invention, the Leptospora kurtosis NC-K143 provides inhibition effects for a variety of bacteria and yeast-like fungi, and particularly provides the best inhibition effects for gram-positive bacteria, such as staphylococcus aureus; the krill NC-K143 disclosed by the invention can be used for preparing a medicine for preventing and treating pathogenic bacteria infection of a human body, and has important significance on reducing the use of a large amount of antibiotics, reducing environmental pollution and reducing the generation of drug-resistant bacteria.

The invention discloses a polyketone compound derived from actinomycetes as well as a preparation method and application thereof, and relates to the technical field of microbial natural product mining and biological medicine, and the key point of the technical scheme is that the invention discloses a novel polyketone compound Strepactone derived from actinomycetes Streptomyces sp. DP0001, and the molecular formula of the novel polyketone compound Strepactone is C25H38O5. The compound is obtained through strain fermentation, ethyl acetate extraction and multi-step chromatographic purification, and the structure is identified through ESI-MS, 1H NMR and 13C NMR. Experiments show that Strepactone has an inhibition effect on staphylococcus aureus and methicillin-resistant staphylococcus aureus and has remarkable inhibition activity on human non-small cell lung cancer A549 cells, the half inhibitory concentration IC50 of the Strepactone is 5.36 mu M, and the Strepactone has no obvious cytotoxic activity on human embryo kidney cells 293T cells at the concentration of 30 mu M. The compound provided by the invention can be used for preparing anti-drug-resistant bacteria drugs and anti-human non-small cell lung cancer drugs, and also provides a structural basis for the development of novel antibacterial and anti-tumor leading drugs.

The invention discloses a photoresponse-essential oil synergistic antibacterial polymer and an application thereof in resisting multiple drug-resistant bacteria. Citronellol (CT) is subjected to an esterification reaction to prepare a CMA monomer, the CMA monomer and a glycidyl methacrylate (GMA) monomer are copolymerized to prepare a polymer PG9C2, and then TBO is introduced to obtain the antibacterial polymer PG9C2T1. The improvement of the antibacterial activity of the TBO is derived from the improvement of the fat solubility of the TBO, the enhancement of the interaction between the TBO and bacterial cells, the improvement of the accumulation of the TBO in the cells, the penetration of the TBO through cell membranes and the play of roles in the cells, the improvement of the utilization efficiency of ROS, and the sterilization by improving the water solubility of the essential oil through the mechanism of destroying bacterial membranes. According to the invention, high-efficiency and low-toxicity drug-resistant bacteria based on natural products are synthesized, the limitation of a single antibacterial mode is broken through, and technical support is provided for developing a novel low-drug-resistance antibacterial therapy.

The invention belongs to the technical field of biological medicine, and particularly discloses application of tea polyphenol combined with meropenem to preparation of a medicine for resisting carbapenem drug-resistant bacteria, and the carbapenem drug-resistant bacteria are animal source blaNDM-5 positive escherichia coli and / or klebsiella pneumoniae. A trace bouillon chessboard method, a cefoxitin hydrolysis test, a time-sterilization curve, a greater wax moth infection model and the like prove that the tea polyphenol and carbapenem antibiotic meropenem generate a good synergistic antibacterial effect, and the tea polyphenol can also inhibit the activity of NDM-5 enzyme; meanwhile, the treatment and protection effects of meropenem on a greater wax moth infection model are improved. It is found for the first time that tea polyphenol can reverse the drug resistance of carbapenem drug-resistant bacteria to meropenem and enhance the antibacterial activity of meropenem to carbapenem drug-resistant bacteria, the synergistic effect is remarkable, use is easy, popularization is easy, and good application value is achieved.

The invention provides a method for synchronously and rapidly detecting Escherichia coli and multiple drug-resistant genes thereof, which comprises the following steps: culturing a sample at night to obtain a bacterial solution to be detected, adding a buffer solution and a metal bath, centrifuging, taking a supernatant, detecting by micro-fluidic, optimizing the extraction process of nucleic acid in the sample, designing an optimal RPA primer probe group, and detecting the multiple drug-resistant genes of the Escherichia coli. A reasonable RPA coupled microfluidic detection system is established, synchronous and rapid detection of the escherichia coli uidA and I-type integrase gene intI1 thereof and drug-resistant genes sul1 and aadA5 is realized, the specificity is high, the sensitivity is high, the reaction time is short, the operation is convenient, and a method support is provided for monitoring and prevention and control of food-borne drug-resistant bacteria.

This invention relates to a 1,3,4-thiadiazole peptide deformylase inhibitor and its preparation and application. The structural formula is shown in formula (1): wherein, R 1 It is n-butyl or cyclopentylmethyl; R 2 The compounds are hydrogen-containing, straight-chain alkanes, cyclic alkanes, aromatic rings, substituted biphenyls, or heterocyclic rings. The 1,3,4-thiadiazole peptide deformylase inhibitors provided by this invention can effectively inhibit bacterial protein synthesis, thereby achieving sterilization. These compounds exhibit excellent inhibitory activity against Gram-positive drug-resistant bacteria, especially the clinically challenging methicillin-resistant Staphylococcus aureus (MRSA). In particular, some preferred compounds show inhibitory activity 4-8 times that of the control standards vancomycin and linezolid. The 1,3,4-thiadiazole peptide deformylase inhibitors provided by this invention also exhibit inhibitory activity against Gram-negative drug-resistant bacteria, especially against drug-resistant Acinetobacter baumannii, known as "superbugs," with an inhibitory activity reaching 0.5 μg / mL, far superior to vancomycin and linezolid.

To provide a detection method capable of rapidly detecting drug-resistant bacteria, bacteria of the genus Staphylococcus, and bacteria of the genus Pseudomonas; a method for providing an index for selecting an antimicrobial agent for bacteria; and a primer set usable in these methods.SOLUTION: The detection method for drug-resistant bacteria, bacteria of the genus Staphylococcus, and bacteria of the genus Pseudomonas according to the present disclosure comprises a step of performing nucleic acid amplification of target genes (1) to (3) and (4) below for a target sample, and detecting drug-resistant bacteria, bacteria of the genus Staphylococcus, and bacteria of the genus Pseudomonas in the sample: (1) the mecA gene; (2) the spa gene of Staphylococcus aureus; (3) the tuf gene and / or the rpoB gene of bacteria of the genus Staphylococcus; and (4) the 16S rRNA gene of bacteria of the genus Pseudomonas.SELECTED DRAWING: Figure 1

The present invention discloses an IS26-based resistance gene control plasmid, engineering bacteria, bacterial agent and application, belonging to the technical field of genetic engineering. The nucleotide sequence of the IS26-based resistance gene control plasmid is shown in SEQ ID NO: 1 or SEQ ID NO: 2. The engineering bacteria and bacterial agent containing the resistance gene control plasmid can be used for the control of resistance genes. By specifically destroying the insertion sequence IS26, the number of resistance genes can be fundamentally reduced and the spread of resistance genes can be blocked, providing a precise solution for the treatment of drug resistance. It has broad-spectrum resistance bacteria control performance and can effectively control gentamicin, tetracycline, chloramphenicol and ampicillin-resistant bacteria in wastewater, with the highest control efficiency reaching 83.76%. The plasmid, engineering bacteria and bacterial agent have low preparation costs, can be used for in-situ treatment of wastewater, have low treatment costs and good effects, and have broad application prospects.

The application provides a preparation method of a high-efficiency piezoelectric coating on a titanium implant surface. The preparation method of the application uses a hydrothermal treatment and an in-situ piezoelectric deposition technology to in-situ synthesize a nano strontium titanate-noble metal piezoelectric coating on the titanium implant surface. The coating can generate a piezoelectric potential and active oxygen through ultrasonic driving to degrade extracellular polymers and kill bacteria. In addition, the piezoelectric coating can also promote the osteogenic differentiation of bone marrow mesenchymal stem cells under piezoelectric stimulation, and finally realize the effects of bioactivity, long-acting drug-resistant bacteria and high-efficiency bone integration in the body. The application can provide a new surface treatment technology and research idea for controlling the drug-resistant bacteria infection on the implant surface, help to break through the limitations of the existing theory and technology in removing bacterial infection on the titanium implant surface, and provide beneficial guidance for the control of bacterial biofilm on other parts and the prevention and treatment of related infectious diseases.

The present invention provides a 7-((5-membered heterocyclic ring)methyl)-8-carboxylic acid-benzo cyclic borate derivative represented by the following formula (I) and its use. This derivative has good inhibitory activity with a broad spectrum against metallo-β-lactamase (MBL) and serine-β-lactamase (SBL) commonly found in clinics, and can be used in the preparation of antibacterial drugs, especially drugs acting against drug-resistant bacteria, as inhibitors of MBL and / or SBL enzymes. In addition, by combining this derivative with β-lactam antibiotics, it has good antibacterial activity against various β-lactam antibiotic-resistant bacteria, has great potential in the preparation of drugs for overcoming double broad-spectrum inhibitors of MBL and SBL and β-lactam antibiotic-resistant bacteria, provides new options for the use of antibacterial drugs, and various future applications can be expected. JPEG2025521595000121.jpg3273