The invention discloses tendon-relaxing antibiosis beef paste which is prepared from the following raw materials in parts by weight: 70-80 parts of beef, 50-60 parts of soybean paste, 20-25 parts of red radish sprouts, 20-25 parts of Chinese toon sprouts, 20-25 parts of Chinese torreya, 1-2 parts of thunberg fritillary bulb, 2-3 parts of typha angustifolia, 1-2 parts of begonia evansiana, 1-2 parts of ternate buttercup root, 1-3 parts of green onion and ginger powder, 1-2 parts of pepper, 0.6-1 part of cinnamon, 8-10 parts of brown sugar, 10-12 parts of walnut oil, 20-24 parts of colza oil, 1-2 parts of food additives and a proper amount of water. On the basis of taking the beef as the main raw material, the red radish sprouts with the functions of helping digestion, regulating vital energy, reducing phlegm, relieving a cough, cleaning lung, smoothing throat and eliminating stasis to subdue swelling are added into the beef paste, the Chinese toon sprouts have the functions of protecting liver, tonifying spleen, enriching the blood, relaxing tendon, diminishing inflammation and preventing virus, the Chinese torreya has the functions of killing insects, removing food retention, lubricating lung to reduce phlegm, lubricating intestines to heal haemorrhoids, tonifying spleen, tonifying Qi and removing stagnation to generate new cells, and the walnut oil has the functions of building the body, beautifying the face, beautifying hair, improving the intelligence, preventing cardiovascular and cerebrovascular diseases and supplementing calcium.