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A kind of natural polysaccharide-based hydrogel and its preparation and application in eye conjunctival repair

A natural polysaccharide and gel technology, applied in medical science, prosthesis, etc., can solve the problems of poor recovery of injectable hydrogel, unsuitable for surgical operation, cytotoxicity, etc., to inhibit the growth of fibroblasts and promote epithelial Effect of cell growth and protection of surgical wound

Active Publication Date: 2017-08-08
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether it is UV light crosslinking (photoinitiator) or the introduction of chemical crosslinking agents, there will be certain potential toxic effects on cells
However, physically cross-linked injectable hydrogels have poor recovery and poor mechanical strength, making them unsuitable for surgical operations.

Method used

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  • A kind of natural polysaccharide-based hydrogel and its preparation and application in eye conjunctival repair
  • A kind of natural polysaccharide-based hydrogel and its preparation and application in eye conjunctival repair
  • A kind of natural polysaccharide-based hydrogel and its preparation and application in eye conjunctival repair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Preparation of maleylated chitosan / mercaptolated hyaluronic acid injectable hydrogel

[0040]Add 0.4g of thiolated hyaluronic acid into 10mL of deionized water, and fully dissolve to obtain liquid A with a mass-volume ratio of thiolated hyaluronic acid of 0.04g / mL; add 0.15g of maleoylated chitosan into 10mL of deionized water , after fully dissolving to obtain maleylated chitosan mass volume ratio of 0.015g / mL liquid B; slowly pour an equal volume of liquid B into liquid A under magnetic stirring, and mix evenly; 58 wt.% sodium β-glycerophosphate solution was added dropwise with stirring to adjust the pH to 7, and then incubated in a constant temperature water bath at 37°C for 24 hours to form a pale yellow transparent gel. The prepared maleylated chitosan / mercaptolated hyaluronic acid injectable hydrogel was tested and analyzed. The gel time measured by the method of inverting the vial is 15min, and the schematic diagram of the gel process is as follows f...

Embodiment 2

[0041] Example 2: Preparation of maleylated chitooligosaccharide / mercaptolated hyaluronic acid injectable hydrogel

[0042] The preparation of maleylated chitosan oligosaccharide: according to chitosan oligosaccharide repeating unit and maleic anhydride mol ratio is the ratio of 2:1, in the there-necked flask that fills 2wt.% chitosan oligosaccharide solution, add maleic anhydride, room temperature N 2 After 24 hours of protection reaction, freeze-dry, wash the freeze-dried sample with acetone solution, and vacuum-dry to obtain maleylated chitosan oligosaccharide.

[0043] Add 0.1g of thiolated hyaluronic acid into 5mL of deionized water, and fully dissolve to obtain liquid A with a mass-volume ratio of thiolated hyaluronic acid of 0.02g / mL; add 0.0375g of maleoylated chitosan oligosaccharide into 5mL of deionized water , after fully dissolving to obtain maleylated chitosan oligosaccharide mass-volume ratio of 0.0075g / mL liquid B; under magnetic stirring, slowly pour an equal ...

Embodiment 3

[0044] Example 3: Preparation of itaconicated chitosan / mercaptolated hyaluronic acid injectable hydrogel

[0045] The preparation of itaconic acylated chitosan: according to the ratio of chitosan repeating unit and itaconic anhydride mol ratio being 2:1, add itaconic anhydride in the there-necked flask that fills 2wt.% chitosan solution, room temperature N 2 After 24 hours of protection reaction, dialyze for 3 days and freeze-dry to obtain the preparation of itaconylated chitosan.

[0046] Add 0.6g of thiolated hyaluronic acid into 10mL of deionized water, fully dissolve to obtain liquid A with a mass-volume ratio of thiolated hyaluronic acid of 0.06g / mL; add 0.36g of itaconylated chitosan into 10mL of deionized water , after fully dissolving to obtain itaconylated chitosan mass-volume ratio of 0.036g / mL liquid B; slowly pour an equal volume of liquid B into liquid A under magnetic stirring, and mix evenly; Add 58wt.% sodium β-glycerophosphate solution drop by drop under stir...

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Abstract

The invention belongs to the field of tissue engineering and discloses chemically-crosslinked natural polysaccharide-based injectable hydrogel, a preparation method thereof and application thereof in tissue engineering materials, especially in conjunctival repair. The preparation method includes the following steps: adding an acrylated natural polysaccharide material water solution into a sulfhydrylated natural polysaccharide material water solution, mixing well, adding a beta-sodium glycerophosphate solution to adjust pH to be neutral, and incubating at temperature of 37 DEG C to obtain the chemically-crosslinked natural polysaccharide-based injectable hydrogel. The hydrogel is controllable in performance, can realize gelation under human physiological conditions, is needless of adding other chemical crosslinking agents, can be used as a tissue engineering material and is especially suitable for conjunctival repair, injectable, quick in reaction, capable of realizing gelation within 5-15min, in-situ in forming, high in surgical operability and automatic in bonding in the process of surgery, stitching for fixing is not needed, and wound surfaces in any shape and at any position can be protected effectively.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a chemically cross-linked natural polysaccharide-based injectable hydrogel, a preparation method thereof, and an application in tissue engineering materials, especially eye conjunctival repair. Background technique [0002] The conjunctiva is an important part of the ocular surface. It is located on the surface of the eyeball and is in direct contact with the outside world. It is vulnerable to various traumas, such as chemical injuries such as acid and alkali in the eyes or thermal burns. For these diseases that lack normal conjunctival tissue, there is still a lack of clinically effective treatments. At present, the commonly used treatment method is to use normal conjunctiva or conjunctival substitutes for conjunctival reconstruction, including autologous conjunctival transplantation, lip mucosal transplantation, amniotic membrane transplantation, and allogeneic con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/52A61L27/54
Inventor 李立华叶碧华孟璐李志文罗学仕李日旺蔡正伟周长忍
Owner JINAN UNIVERSITY
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