Application of histatin 1 (Hst1) polypeptide in preparation of composite materials for promoting repair of large-area skin defects

A composite material, skin defect technology, applied in the field of biomedicine to achieve the effects of accelerating healing speed, inhibiting inflammatory response, good biocompatibility and biosafety

Active Publication Date: 2018-11-13
HANGZHOU HUIBO SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, histamine 1 (Hst1) has been deeply studied in the field of biomedicine, and it has various functions such as promoting cell adhesion, extension, migration, vascular metaplasia, and anti-inflammation, but whether Hst1 can be applied to large areas There is no relevant report on skin defect repair

Method used

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  • Application of histatin 1 (Hst1) polypeptide in preparation of composite materials for promoting repair of large-area skin defects
  • Application of histatin 1 (Hst1) polypeptide in preparation of composite materials for promoting repair of large-area skin defects
  • Application of histatin 1 (Hst1) polypeptide in preparation of composite materials for promoting repair of large-area skin defects

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Research on the promotion of cell adhesion, extension and angiogenesis by Hst1 polypeptide

[0063] (1) Bone marrow mesenchymal stem cells (purchased from Cyagen Biosciences Inc (USA), batch number 170221I31) were inoculated in 96-well plates at a density of 5×10 per well. 3 Bone marrow mesenchymal stem cells (BMSCs) were added to 50 μl serum-free medium (a-MEM, Gibco) containing a final concentration of 10 μM Hst1 polypeptide, and the control group was pure serum-free medium (a-MEM, Gibco), cultured for 1.5 h, Phalloidin staining after 3h, observing cell adhesion and extension.

[0064] (2) BMSCs were seeded in a 96-well plate at a density of 5×10 per well 3 Add 50 μl of serum-free medium containing a final concentration of 10 μM Hst1 polypeptide to culture 1d (day), 3d and 5d, and then mix 90 μl of culture medium (a-MEM, Gibco) and 10 μl of CCK-8 solution at each time point (Nippon Dojin Chemical Co., Ltd.) was added to each well, and incubated again at 3...

Embodiment 2

[0074] Example 2: Research on Hst1 polypeptide promoting skin defect repair in rats

[0075] (1) Construction of a large-area skin defect model in SD rats

[0076] SD rats (5 in total, weighing 250-300 g, purchased from the Animal Experiment Center of Southern Medical University) were anesthetized by intraperitoneal injection of 3% (w / v) pentobarbital sodium, placed prone on the operating table, and fixed their limbs , back shaved, 1% (w / v) iodine sterilized towels. A skin puncher was used to make a circular full-thickness skin defect with a diameter of 5 mm on the dorsal side. Within 3 days after the operation, gentamicin (injection volume: 4 mg / kg) was injected daily to prevent infection, and carprofen (injection volume: 12.5 mg / kg) was injected every day. mg / kg) for analgesia, and normal feeding in separate cages.

[0077] (2) Repair of skin defect in rats by Hst1 polypeptide

[0078] Using SD rats as their own control, one side is the experimental group, implant the pre...

Embodiment 3

[0082] Example 3: Hst1 polypeptide-loaded thiolated chitosan hydrogel promotes skin defect repair in rats

[0083] (1) The preparation method of the thiolated chitosan hydrogel loaded with Hst1 polypeptide is the same as that in Example 1.

[0084] (2) Repair of rat skin defects by thiolated chitosan hydrogel loaded with Hst1 polypeptide

[0085] Adopt SD rat (model construction method is the same as embodiment 1) self control, one side is experimental group, injects the hydrogel of loading Hst1 polypeptide thiolated chitosan, the hydrogel of loading Hst1 polypeptide thiolated chitosan (per case Skin defect injection (40ul hydrogel) was injected into the skin defect (Ф=5mm) of SD rats; the other side was the control group; the control group (Con) received no special treatment. The result is as Figure 5 As shown, it shows that the thiolated chitosan loaded with Hst1 polypeptide can obviously promote skin repair.

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Abstract

The invention discloses application of histatin 1 (Hst1) polypeptide in preparation of composite materials for promoting repair of large-area skin defects. The Hst1 polypeptide is found to have the characteristics of promoting cell adhesion and vascularization and inhibiting inflammatory response. The Hst1 polypeptide can be loaded on a variety of biological materials, and can be prepared into dressing, injection, ointment, powder, hydrogel, membranes, sponges, fiber scaffold materials and the like, which can effectively promote the healing of the large-area skin defects, by being compounded with bioactive composite materials, wherein large-area skin defects include traumas, burns, skin ulcer caused by tumor resection or diabetes, and the like. The Hst1 polypeptide provided by the invention is human-derived polypeptide, and has good biocompatibility and biosafety. When being used for the preparation of the composite materials for promoting the repair of the large-area skin defects, theHst1 polypeptide has an important medical value and a good industrialization prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and particularly relates to the application of histamine-1 polypeptide in the preparation of composite materials for promoting the repair of large-area skin defects. Background technique [0002] Large full-thickness skin defects are common in trauma, burns, tumor resection, and skin ulcers caused by diabetes. Large-area full-thickness skin defects cannot heal on their own, and autologous skin grafts and flaps are needed. However, the above methods have limited sources and require secondary surgery. Allogeneic skin grafts and flaps have risks such as immune rejection. Therefore, a large number of artificial dermal implants have been used for skin defect repair, but these materials have not finally obtained the desired therapeutic effect. The unsatisfactory treatment of skin defects is due to the lack of blood vessel supply in large-scale defect skin, which cannot provide sufficient oxygen and nutrien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K9/06A61K47/36A61L26/00A61P17/02A61P29/00A61P31/02
CPCA61K38/1703A61K47/36A61L26/0023A61L26/0047A61L26/008A61L26/009A61P17/02A61P29/00A61P31/02A61K9/0014A61K9/06A61L2300/41A61L2300/412A61L2300/404A61L2300/252A61L2300/604C08L5/08
Inventor 林振柳毅王晶李立华李日旺吴刚
Owner HANGZHOU HUIBO SCI & TECH CO LTD
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