Small ruminant semen freezing dilution solution

Abstract

The invention relates to a small ruminant semen freezing dilution solution. Every 100ml of the small ruminant semen freezing dilution solution comprises the following components: 2.71g of tris(hydroxymethyl)aminomethane, 1.4g of citric acid, 1.0g of monosaccharide, 5-20ml of fresh egg yolk, 0-10ml of permeability protection agent, 100,000IU of penicillin, 100,000IU of streptomycin, 50-600mMol of inositol compound, 0.1-50mu Mol of resveratrol and the balance of ultra-pure water. In the invention, the resveratrol is successfully applied to the field of small ruminant semen freezing for the first time, the quality of the frozen semen is improved, the cost is low, the chemical properties are stable, and the application method is simple and easy to operate.

Description

technical field [0001] The invention relates to the technical field of animal reproductive physiology and reproduction, in particular to a small ruminant seminal fluid freezing diluent. Background technique [0002] Although the research on the cryopreservation of livestock semen has been carried out for more than 50 years, the viability of the thawed semen is only about 30%-50%, and the non-return rate and litter rate after artificial insemination are significantly lower than that of fresh and low-temperature frozen semen. During the freezing process of animal semen, the damage to sperm cells is mainly caused by the physical damage caused by ice crystals formed inside and outside the cell membrane. The composition of the diluent can reduce the damage of freezing to sperm, mainly by adding certain osmotic organic solvents, sugars, egg yolks, ice crystal inhibitors, etc. to the semen diluent. The viability of sperm cells after freezing cannot inhibit the formation of ice cry...

AI Technical Summary

Problems solved by technology

However, although ice crystal inhibitors of inositol compounds can alleviate the adverse effects of ice crystals on sperm plasma membrane and mitochondria during freezing and thawing, the damage to sperm cell structure is still serious

Especially due to the particularity of the semen cryopreservation procedure, the low temperature balance of semen at 0-5°C before freezing is very critical for the survival of frozen sperm. Usually this process takes 2-4 hours, but the metabolic activity of sperm at 0-5°C is not affected. Not completely stopped, its metabolites may cause severe oxidative damage and apoptosis to spermatozoa, eventually causing damage to cell structure

The conventional practice in this field is to add certain antioxidants (vitamin C, vitamin E, superoxide dismutase, catalase, glutathione peroxidase, etc.) However, the metabolic mechanism of sperm cells at low temperature equilibrium at 0-5°C is unclear, and the mechanism of cell damage is also unclear. The damage to sperm of different animals is also inconsistent, and the sperm cells are tiny and fragile, and the sperm cells are small and fragile. A slight change in the compatibility and dosage of the two chemical reagents will result in very different freezing results. Therefore, it is not possible to reduce the damage of sperm cells at low temperature equilibrium at 0-5°C by simply adding antioxidants, nor can a single combination be used. Methods for all species of animals

[0005] For the livestock breeding industry, excellent male animals are selected through several rounds of selection. In order to maximize the use of excellent male animals to expand the herd, the common practice in the industry is to inseminate as much semen as possible from excellent male animals to estrous female animals, which is the most scientific The most economical method is to dilute the collected semen and then freeze it, and artificially inseminate the female animal after thawing when it is used. This can quickly spread the genes of excellent male animals. However, this method is only generally implemented in the cattle industry and has already However, in terms of small ruminants, including goats and sheep, due to the characteristics of the species, the ejaculation volume of sheep is relatively small, and the sperm are more sensitive to freezing damage. The use and promotion of semen freezing technology in the sheep industry is far away. It is not as good as the cattle industry. In practice, many places still use the method of insemination of live sheep, which greatly limits the expansion of excellent rams, making it impossible for the genes of excellent rams carefully selected for many years to spread rapidly, and the improvement of sheep flocks is slow.

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