87 results about "Sperm density" patented technology

The invention discloses a special feed additive for conditioning the breeding performance of artificially inseminated breeder cocks and application thereof. The special feed additive for conditioning the breeding performance of artificially inseminated breeder cocks is prepared from a composite traditional Chinese herbal additive and a nutritional feed additive according to a weight ratio of 1:1, wherein the composite traditional Chinese herbal additive comprises 7-10 percent of epimedium, 7-10 percent of actinolite, 7-10 percent of yam, 7-10 percent of prepared rhizome of rehmanniat, 5-8 percent of medlar, 5-8 percent of cistanche salsa, 5-8 percent of dodder, 5-8 percent of raspberry, 5-8 percent of plantain seeds, 5-8 percent of japanese teasel, 5-8 percent of liriope, 3-4 percent of Dangshen, 3-4 percent of morinda officinalis, 3-4 percent of astragalus, 3-4 percent of angelica sinensis, 3-4 percent of atractylodes and 3-4 percent of glycyrrhiza, and the percent sum of the raw materials is 100 percent. The nutritional feed additive comprises 35-45 percent of alfalfa meal, 20-25 percent of folium cortex eucommiae powder, 20-25 percent of mulberry leaves powder, 4-9 percent of bone meal, 5-10 percent of saleratus and 0.5-1 percent of trace elements and composite vitamin additive, and the percent sum of raw materials is 100 percent. After the special feed additive is applied, the average ejaculate volume of breeder cocks is improved by 17.6 percent, the sperm activity is improved by 16.1 percent, and the sperm density is improved by 14.2 percent.

A natural vegetable feed additive for stud ram relates to a feed additive for stud ram, which solves the problems of body situation reducing, libido and semen quality reducing of the ram during the service period, not high conception rate of ewe and service life reducing of the ram during the service period. The invention employs the prescription of the following components, which namely consists of Epimedium, dodder, actinolite, campanumaea pilosula, Rehmannia Glutinosa and Charred Triplet. The product of the invention enhances the sexual behavior of the ram, and remarkably improves the indexes like the sperm viability, sperm density, and ejaculatory amount, does not have side effects to the cloud characteristic of the sperm, the pH, the sperm abnormal rate, and the abnormal rate of acrosome, and can simultaneously prolong the service life of the stud ram by 1 to 1.5 years.

The invention belongs to the technical field of porcine molecular marker screening, and in particular relates to porcine NTF3 promoter region SNP as a molecular marker for the boar breeding traits and applications of the porcine NTF3 promoter region SNP. The molecular marker is cloned from the 5' flank promoter fragment of the porcine NTF3 gene, the nucleotide sequences of the molecular marker are shown as SEQ ID NO:1 and SEQ NO:2, allele mutation of A/G and G/A exists at the 92nd basic group site of the sequence shown as SEQ ID NO:1 and SEQ NO:2, and the allele mutation causes the polymorphism of MvaI-RFLP. The marker is utilized for carrying out correlation analysis on the boar breeding traits of large white pigs and duroc pigs. The breeding traits comprise the boar sperm motility and the sperm density trait. The invention further discloses a typing detection method of the marker, and therefore, a novel marker is provided for selecting the boar breeding traits. The marker can be used for evaluating the boar breeding traits and researching the genetic improvement on the breeding boar breeding performance.

The invention discloses boar complete formula feed for improving semen quality. The boar complete formula feed for improving semen quality is prepared from the following raw materials in parts by weight: 60-65 parts of corn, 10-16 parts of bean pulp, 3-8 parts of soybean hull, 5-8 parts of wheat bran, 0.5-1 part of nucleotide, 1-3 parts of imported fishmeal, 0.8-1.1 parts of calcium hydrophosphate, 0.6-0.9 part of mountain flour, 0.1-0.3 part of lysine, 0.1-0.05 part of threonine, 0.2-0.4 part of baking soda, 0.01-0.05 part of selenium yeast, 0.2-0.4 part of salt, 0.1-0.15 part of choline chloride, 0.1-0.5 part of N-carbamylglutamic acid and 1-4 parts of boar premix. The complete formula feed disclosed by the invention has the advantages of improving the ejaculatory amount, sperm motility and sperm density of boars and enhancing the heat-stress resistance of the boars.

The invention discloses a mixed feed for improving antioxidant activity of the sperm of a boar. The mixed feed is characterized by comprising the following raw materials by weight: 40 to 60% of corn, 5 to 20% of fish meal, 5 to 20% of wheat, 10 to 25% of wheat bran, 0.2 to 5% of a soybean small peptide additive, 0.05 to 2% of taurine, 2 to 10% of soybean meal, 2 to 8% of plant oil, 1 to 2% of a calcium element additive, 0.1 to 0.8% of salt, 0.05 to 0.5% of N-carbamylglutamic acid, 0.1 to 0.3% of a composite trace element additive, 0.03 to 0.08% of a composite vitamin additive, 0.005 to 1% of anthocyanidin, 0.05 to 2% of tea polyphenol, 0.5 to 1.5% of amino acid and 0 to 5% of bentonite. The mixed feed is applicable to the boar, can effectively increase the amount of boar seminal fluid, improve sperm vitality and sperm density, reduce the rate of sperm deformation and enhance antioxidant activity of the sperm of the boar and is of great significance to improvement of the quality of boar seminal fluid.

The invention discloses a special diet edible food formula for improving sperm quality of males and a preparation method of the formula and belongs to the field of special diet edible food. Whey protein, sea cucumber polypeptide, oyster polypeptide, bone collagen peptide, soybean peptide, corn oligopeptide, wheat oligopeptide and casein phosphopeptides are used as basic raw materials, edible and medicinal raw materials including raspberries, fructus lycii, cortex cinnamomi and the like are added, wall-broken piney flower powder, spirulina platensis powder, a roxburgh rose extract, a maca extract, cordyceps militaris and fructooligosaccharide are matched, so that the special diet edible food is formed. The formula product is formed through steps of raw material pretreatment (cleaning, enzymolysis, extraction and the like), crushing, sieving, mixing and the like. The formula components are all food materials, and the prepared product has functions of increasing sperm density, increasingsperm motility rate, reducing sperm aberration rate and improving immunity and fatigue resistance. The product has high safety, has no toxic or side effects, has low fishy smell and bitterness and canbe eaten for a long term.

The invention provides a two-layer percoll density gradient centrifugal separation method of boar sperms. The method is suitable for normal sperm specimen and sperm specimen with low sperm density or low sperm motility, and is a simple, convenient and fast sperm treatment method. The method can be used for remove leucocytes, bacteria, fragments and anti-sperm antibodies in the sperms and improving the delayed sperm liquefaction, the sperm in-vitro capacitation and the like, so that the sperms can effectively fertilize ova to improve the pregnancy rate, and the percentage of the sperms with motility and normal shape in the obtained sperms is about 95%. The method provided by the invention is suitable for screening sperms with motility from fresh boar sperms and diluted sperms, thus providing high-quality sperms for the study of boar sperm capacitation mechanism and in-vitro fertilization.

The invention provides an application of testosterone in low dosage for preparing medicine for treating asthenospermia. To chronically take testosterone in low dosage can lead the sperm density to decline and the sperm mobility to rise, enhance the sperm mobility of a patient who has the asthenospermia, and improve the conception rate of the patient who has the asthenospermia.

The invention provides a traditional Chinese medicine health-care feed for the breeding boars and a preparation method of the traditional Chinese medicine health-care feed, and belongs to the technical field of feeds. The traditional Chinese medicine health-care feed for the breeding boars is prepared from the following raw materials in parts by weight: corns, bean pulp, folium cortex eucommiae, a breeding boar composite premix, complex probiotics, L-carnitine and a composite traditional Chinese medicine additive, wherein the composite traditional Chinese medicine additive is prepared from herba epimedii, actinolite, fructus lycii, radix rehmanniae praeparata, white paeony roots and cornus officinalis. The preparation method comprises the following steps: smashing the corns, the bean pulp, the folium cortex eucommiae and the composite traditional Chinese medicine additive to obtain mixed powder, mixing the mixed powder with the breeding boar composite premix, the complex probiotics and the L-carnitine, and carrying out granulation. The traditional Chinese medicine health-care feed for the breeding boars, which is prepared through the preparation method, can enhance the immunity of the breeding boars, improve the endurance and the physical power, increase the sexual desire of the boars, obviously increase the semen volume, the sperm density and the sperm survival rate of the breeding boars and prolong the life of the breeding boars, and residues, drug resistance and toxic and side effects are not liable to occur; the traditional Chinese medicine health-care feed is safe and reliable.

The invention discloses a pig feed capable of improving the quality of breeding boar semen fluid and a preparation method thereof. The feed is prepared from the following materials by weight parts: 550-600 parts of corn, 100-150 parts of puffing bean pulp, 200-250 parts of bran, 40-50 parts of a premix, 10-15 parts of calcium hydrophosphate, 3-5 parts of salt, 2-3 parts of sodium bicarbonate, 3-5 parts of attapulgite, 5-8 parts of silene aprica, 4-6 parts of herba epimedii, 2-3 parts of pumpkin seeds, 3-4 parts of viscum album, 1-3 parts of tuber onion seeds, 1-2 parts of human placenta, 2-3 parts of semen cuscutae, 10-15 parts of chestnut shells and 5-10 parts of spartina anglica. The feed prepared by using the method disclosed by the invention not only can provide various nutrients needed by the healthy growth of breeding boars and has good feed palatability, but also can obviously improve the semen volume, the sperm density and the sperm survival rate; meanwhile, the acrosome abnormal rate and the rate of sperm deformation are obviously reduced.

The invention discloses a Chinese herbal medicine feed additive capable of improving the quality of gander semen. The feed additive is prepared from the following raw materials in parts by weight: 6-10 parts of actinolite, 6-10 parts of herba epimedii, 5-9 parts of cistanche, 5-9 parts of semen cuscutae, 5-9 parts of teasel root, 4-8 parts of codonopsis pilosula, 4-8 parts of eucommia ulmoides, 4-8 parts of astragalus membranaceus, 3-7 parts of licorice, 1-3 parts of sanguisorba officinalis, 1-3 parts of hairyvein agrimony, 1-3 parts of honeysuckle and 1-3 parts of semen plantaginis. A preparation method of the feed additive includes the steps of weighing, mixing and pulverizing the raw materials. The addition quantity of the Chinese herbal medicine feed additive is 2% of total quantity of a feed when the feed is fed to ganders; the feed additive is specially added to the feed for ganders, after the ganders eat the feed, the semen volume, the sperm density and the sperm activity of the ganders are remarkably improved, the mating capacity of the ganders is enhanced, and the hatching egg fertility rate and the hatchability are remarkably improved; besides, the ganders fed with the Chinese herbal medicine feed additive have certain disease resistance.

The invention discloses a compound feed for breeding boars. The compound feed comprises the following components in percentage by weight: 60-66% of corn flour, 3-5% of fish meal, 4-7% of fermented soybean meal, 10-15% of soybean meal, 10-11% of bran, 0.3% of multiple minerals, 0.5% of table salt, 0.04% of multiple vitamins, 0.12% of choline, 1.2% of dicalcium phosphate, 0.02% of phytase, 1.2% of stone powder, 0.12% of lysine, 0.03% of a probiotics compound and 0.5-1.0% of a Chinese herbal medicine compound for producing sperm. The compound feed disclosed by the invention is reasonable in formula design, provides comprehensive balanced nutrition for breeding boars, and creatively provides the Chinese herbal medicine compound for producing sperm, so that difficult problems that the semen quality is reduced and the sexual desire of the boars is poor caused by large sperm collection intensity of the breeding boars are solved; and the Chinese herbal medicine compound for producing sperm has the effects of nourishing the liver, nourishing the kidney, benefitting sperm, replenishing blood, reinforcing the spleen, invigorating the stomach and the like, so that after the breeding boars eat the compound feed, the sexual desire of the breeding boars is strengthened, the sperm viability, the sperm density and the semen volume are obviously improved, and the sperm abnormality rate is obviously reduced.

The present invention discloses a breeding cattle feed to improve reproductive capacity. The feed consists of 300 parts of soybean meal, 50 parts of corn meal, 15 parts of fish bone meal and 6 parts of traditional Chinese medicine additives, and the traditional Chinese medicine additives are prepared from: 2-5 parts of herba houttuyniae, 1-3 parts of radix pulsatillae, 2-4 parts of poria cocos, 1-4 parts of rhizoma atractylodis macrocephalae, 3-5 parts of radix salviae miltiorrhizae, 1-3 parts of fructus aurantii, 2 parts of medicated leaven, 3-4 parts of herba leonuri, 2-5 parts of radix codonopsis, 1-3 parts of herba epimedii, 3-4 parts of radix et caulis acanthopanacis senticosi and 3-4 parts of hawthorn fruits. The feed is balanced in nutrition, can significantly enhance the physique of bulls, improve the reproductive capacity, increase the single ejaculate volume and sperm density of the bulls, and has a very good effect to help farmers increase income.

The invention belongs to the field of livestock raising and discloses a method for bringing forward the sexual maturity of a cock. The method for bringing forward the sexual maturity of the cock comprises the following steps that before the hatching, the small end of a hatching egg is sterilized, a hole is slightly drilled in the sterilized part by a toad spinal cord puncture needle, a trace sample feeder is used for introducing 100 muL 0.5 to 2ng/muL kisspeptin-10 injection reagents into egg white through the drilled hole, and an opening is sealed with paraffin wax. The kisspeptin-10 is used for processing the hatching chicken egg for the first time, in addition, the processing concentration of kisspeptin-10 is optimized, results show that when the method provided by the invention is used for Kp-10 (kisspeptin-10) egg injection, the body weight of the cock can be obviously increased, the sperm density of the cock is improved, the sexual behavior of the cock is increased, and the obvious influence is realized on the T content in the blood serum of the 14-week and 24-week cock.

The invention relates to a method for detecting the number of effective sperms in porcine seminal plasma. The method comprises the following operation steps: 1, arranging a counting cell with 100 small checks on a counting plate, wherein the depth of the counting cell is 0.01mm, and the length and width of the counting cell are respectively 0.1m; 2, preheating the counting plate to 37 DEG C, and covering a piece of cover glass on the counting cell; 3, dripping the to-be-detected porcine seminal plasma between the cover glass and the counting cell, and naturally sucking the porcine seminal plasma into the counting cell; and 4, performing microscope detection, and performing statistical analysis on sperm density, sperm motility and the number of effective sperms. The method disclosed by the invention is easy to operate and suitable for field detection of seminal plasma before use in a pig farm without an automatic semen analyzer, and only simple instruments and components need to be equipped; the calculation method in the invention is simple, and the number obtained by dividing the amount of sperms in a single small check by 10 refers to the sperm density during seminal plasma detection; and moreover, the sperm motility is obtained by dividing the amount of sperms making a linear motion in a single small counting cell by the total number of sperms in the single small counting cell, and the number of effective sperms is obtained by multiplying the sperm motility by the total amount of sperms in the to-be-detected porcine seminal plasma.

The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a traditional Chinese medicine composition for treating asthenospermia and oligospermia, anda preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from certain raw materials according to a ratio, thus having a better treatment effect; after a patient with the asthenospermia and oligospermia is treated by using the traditional Chinese medicine composition for 70 days, the ratio of sperms with level a reaches up to 22% or above, the ratio of sperms with level a+b reaches up to 60% or above, the sperm activity rate is as high as 68% or above, and the sperm density is as high as 35% or above; the effective rate of the traditional Chinese medicine composition for patients with the asthenospermia and oligospermia reaches up to 98% or above, and the pregnancy rate of spouses is as high as 70% or above.

The invention relates to a method for in vitro capacitation of rabbit sperms and a nutrient solution formula. In the invention, 10 mum mol/L of EGCG is added in a BO capacitation liquid for sperm in vitro capacitation treatment, and 10 mum mol/L of EGCG is added in the BO capacitation liquid, the formula of the a BO capacitation liquid comprises the following components by weight: 6.5453mg/mL of NaCl, 0.2997mg/mL of KCL, 0.1145mg/mL of NaH2PO4.H2O, 0.1057mg/mL of MgC12.6H2O, 0.330mg/mL of CaCl2.2H2O, 2.518mg/mL of glucose, 4% of 0.2% phenol red, 100IU/mL of penicillin, 100IU/mL of streptomycin, 3.1080mg/mL of NaHCO3, 0.1380mg/mL of sodium pyruvate, 20mum g/mL of heparin and 20mg/mL of BSA. The method for in vitro capacitation of rabbit sperms overcomes the defect of mass oxyradical accumulation generated by sperm metabolism due to large sperm density during a current sperm in vitro capacitation process. The method uses adult New Zealand white male rabbit, different concentration EGCG (0mumM, 10mumM, 15mumM, 20mumM and 25mumM) can be added in the BO capacitation liquid, influence of the BO capacitation liquid to the rabbit sperm capacitation effect is researched so that optimum EGCG addition concentration is obtained, and thereby a rabbit in vitro capacitation culture system with high efficiency is established.

Provided is dry and hard particle forage for rams. The dry and hard particle forage is prepared from corn, bean pulp, dry wine residue powder, cornstalk powder, alfalfa meal, fish meal, sweet potato residues, oats, Chinese herb powder, baking soda, calcium hydrophosphate and table salt. The dry and hard particle forage has the advantages that nutrition balance is achieved, and picky eating can be effectively avoided; herba epimedii, semen cuscutae and Chinese chives can improve the breeding capacity of the rams, and the fish meal and the dry wine residue powder can improve the palatability of the forage and increase the forage intake; the dry and hard particle forage can guarantee normal rumination of the rams and greatly reduce energy consumption of rumination actives of the rams; the Chinese herb powder can improve the immunity and breeding capacity of the rams and reduce epidemic diseases. In a contrast test, comparison between a ram test group fed with the dry and hard particle forage and a control group fed with common sheep feed shows that the ejaculation amount and the sperm density are significantly increased, and the sperm motility rate is significantly increased; during the test, the rams take the forage actively and have vigorous sex drive, and therefore the breeding capacity is greatly improved.

The invention relates to the technical field of livestock breeding, in particular to a breeding method of improving reproductive performance of Angus bulls. The breeding method includes: on the basis of daily breeding management, feeding calves 7 days after weaning with a zinc-contained preparation, and well regulating secretion of gonad activity and sex hormone of the bulls. The zinc-contained preparation is fed after weaning and before mating, modes of massage, acupuncture and the like are adopted, and dosage, drug delivery way, number of times, preparation content and daily feed nutrients are controlled, so that zinc element is sufficiently absorbed by each part of bodies, absorption and digestion are promoted, and metabolism level of multiple hormones in the bodies of male breasts is regulated; meanwhile, absorption conversion of the bodies on the feed is promoted, content of adverse hormones in the bodies is reduced, sperm density and sperm activity are improved, rate of sperm deformation is reduced, and growing development of testis is promoted; liver steroid metabolism is further improved, and sex steroid receptor level is regulated.

The invention belongs to the field of biomedicine and health, and specifically relates to a unicellular algae fat-soluble substance for increasing male characteristics of males and applications thereof. The main active component of the fat-soluble substance is composed of natural carotenoid and oxidation derivatives thereof, or micro algae powder rich in the carotenoid and oxidation derivatives thereof. The natural fat-soluble mixed substance rich in carotenoid and oxidation derivatives thereof is extracted from unicellular algae, which is obtained though cell engineering large-scale culture. Furthermore, novel healthcare and medical products and biological preparations, which can improve or strengthen the male functions, are developed on the basis of feeding and verification on experiment animals, and the products and biological preparations are used to improve or strengthen certain male characteristics of males such as testis weight, stored sperm number and density in epididymis, and the like.

The invention relates to the field of foods, and specifically relates to a composition and an application thereof. The composition or a common food provided by the invention is capable of increasing amount of semen, shortening semen liquefaction time, increasing density of sperm and improving motility of sperm so as to significantly improving reproductive capacity of the male.