13 results about "Sperm density" patented technology

This invention discloses a traditional Chinese medicine composition for treating male infertility due to liver stagnation and kidney deficiency, its preparation method, and its application, belonging to the fields of traditional Chinese medicine pharmaceutical technology and andrological reproductive health technology. The composition is formulated with Bupleurum chinense, Tribulus terrestris, Cynomorium songaricum, Cuscuta chinensis, Cornus officinalis, and Epimedium brevicornu in specific weight proportions, following the principles of soothing the liver and relieving stagnation, tonifying the kidney and replenishing essence, and unblocking the meridians to treat impotence. This invention employs an alcohol-water dual-phase fractional extraction process, and can be prepared into various dosage forms such as granules, capsules, and tablets. Pharmacological experiments have confirmed that the composition of this invention can increase sperm density and progressively motile sperm rate, improve penile tissue nitric oxide synthase (NOS) activity, regulate reproductive hormones, and reduce testicular oxidative damage; serum pharmacochemistry and metabolomics have clarified the active components entering the bloodstream and their pathways of action. This invention features a refined formulation, abundant medicinal resources, and simple preparation, making it suitable for large-scale production and clinical application in treating male infertility due to liver stagnation and kidney deficiency.

The invention discloses a traditional Chinese medicine composition for preventing and treating light and moderate varicocele and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 12 to 30 parts of radix paeoniae rubra, 9 to 24 parts of peach kernel, 9 to 24 parts of spina gleditsiae, 9 to 24 parts of radix cyathulae, 6 to 18 parts of cassia twig, 9 to 24 parts of fructus forsythiae, 6 to 18 parts of thunberg fritillary bulb, 6 to 18 parts of rhodiola rosea, 6 to 18 parts of semen aesculi and 3 to 15 parts of raw liquorice. After the traditional Chinese medicine composition provided by the invention is adopted for treatment, the spermatovenous reflux time is remarkably shortened, the semen quality including semen volume, sperm density and sperm motility is remarkably improved, the total effective rate of treatment is higher than that of other prescriptions, the living quality of a patient is remarkably improved, the medicine safety is high, and the varicocele can be effectively treated.

This invention relates to the field of sperm detection technology, specifically disclosing an automated sperm density detection chamber integrating sample mixing and temperature control functions. The chamber comprises, from bottom to top, a light-transmitting bottom layer, a microchannel layer, and a light-transmitting cover layer. The light-transmitting bottom layer has, from bottom to top, a glass substrate layer, an ITO heating electrode layer, a first light-transmitting insulating layer, a first ITO mixing electrode layer, and a second light-transmitting insulating layer. The light-transmitting cover layer has, from top to bottom, an upper glass surface layer, a second ITO mixing electrode layer, and a third light-transmitting insulating layer. The microchannel layer forms microchannels, and the light-transmitting cover layer has an inlet port and an exhaust port communicating with the microchannels. This invention achieves stable constant temperature control of the semen sample within the microchannels, effectively avoiding the adverse effects of environmental temperature fluctuations on sperm characteristics and detection results. Furthermore, it enables in-situ automatic mixing of the semen sample, improving the accuracy and repeatability of sperm density detection results.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a pig semen quality trait gene PSMD11, a primer pair and application of the SNP molecular marker and the primer pair, and relates to the technical field of pig molecular markers. The quality traits of the boar semen are semen volume, sperm density, effective sperm density, forward movement activity, sperm activity and sperm malformation rate traits; the molecular marker is located in a No.13 intron of a pig PSMD11 gene, the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table, and G.54 Agt exists at the 54bp position of the sequence; the T basic group is mutated. The invention also provides a primer pair for amplifying the molecular marker and a method for breeding by using the molecular marker. The SNP molecular marker in the pig PSMD11 gene is found to be related to the sperm volume, sperm density, effective sperm density, forward movement activity, sperm activity and sperm malformation rate characters of the pig for the first time, so that the SNP molecular marker can be used for marker-assisted selective breeding of the pig, the early screening of the quality characters of the sperm of the pig is realized, and the screening method is simple and rapid.

The invention belongs to the technical field of livestock breeding, and discloses composite insect powder, composite insect powder zymolyte and a feed additive for improving the quality of breeding male poultry semen. The composite insect powder is tenebrio molitor powder, hermetia illucens powder and cricket powder. Furthermore, the composite insect powder is adopted for enzymolysis, and the effect after enzymolysis is better. After the composite insect powder and the composite insect powder zymolyte are used as feed additives to be added into breeding male poultry feed for feeding, core reproductive performance indexes such as sperm motility and sperm density can be remarkably improved, the sperm motility can reach 86% or above, the sperm density can reach 4 * 10 < 9 > / mL or above, and the breeding male poultry can be fed with the composite insect powder zymolyte. And the compound insect powder has obvious multiple compound effects of yellow mealworms, hermetia illucens and crickets. The compound insect powder is used as a feed additive, the breeding male poultry semen quality is comprehensively improved through multi-dimensional indexes, the raw materials are easy to obtain, use is easy and convenient, and the compound insect powder feed is especially suitable for popularization of large-scale breeding poultry breeding.

The invention provides application of squalene in preparation of a medicine for treating testis injury, and belongs to the technical field of medicines. The research finds that squalene can be used for preparing the medicine for treating sperm quality reduction caused by heat stress. The squalene has a remarkable improvement effect on sperm indexes in three aspects of sperm density, sperm motility, sperm malformation rate and the like. An in-vitro experiment result shows that squalene has relatively high oxidation resistance, can protect reproductive organs from being influenced by heat stress, and has certain oxidation resistance and capability of protecting testis from being injured.

The invention belongs to the technical field of biology, and particularly relates to a method for improving the cryopreservation effect of donkey sperms, which comprises the following steps: selecting a healthy breeding male donkey with vigorous sexual desire, and collecting the sperm by using a strictly disinfected false vagina; seminal fluid quality detection: immediately evaluating sperm motility of the collected seminal fluid under a 37 DEG C constant-temperature phase contrast microscope; measuring the sperm density by using a sperm density instrument; diluting the semen, and freezing the semen by adopting a programmed cooling method; unfreezing the seminal fluid, taking out the thin tube from the liquid nitrogen, immediately putting the thin tube into a constant-temperature water bath at 38 DEG C, slightly shaking, taking out the thin tube after the seminal fluid in the thin tube is completely unfrozen, wiping water drops on the surface of the thin tube by using sterile gauze, and transferring the seminal fluid into a sterile test tube for later use. According to the invention, sperm motility and fertilization capability are improved: sperm collection and treatment processes are optimized, a proper freezing diluent formula is screened, and freezing is carried out by adopting a programmed cooling method, so that damage of sperms in freezing and unfreezing processes is effectively reduced, and sperm motility and fertilization capability after cryopreservation are remarkably improved.

The invention provides a model for studying testis diminution, relates to the technical field of biomedicine, and has the technical key points that Papd7 is a key gene for testis growth and development of male mice through specific experiments. Specifically, after Papd7 is knocked out in a mouse body, compared with a control mouse, the weight of a Papd7 deficient mouse is normally developed, testis can only be developed to half of the size, and correspondingly, the sperm density and activity are only half of those of the control group; and the sperm morphology of the defective mouse is abnormal. In mechanism, testis section staining finds that the testis of the knockout mouse become smaller due to the loss of sperm cells in seminiferous tubules; disorder of various hormones is also one of important reasons. Experimental results show that the Papd7 is a key gene for growth and development of the testis of the mouse, the growth defect of the testis of the mouse can be caused by damaging the function of the Papd7 in the mouse through a CRISPR-Cas9 technology, and the Papd7 is a useful model for researching the testis reduction.

The invention discloses a semen diluent for improving the production efficiency of pig semen, which comprises a basic solution, a nutritional agent, a buffer agent and an electrolyte, and also comprises an antioxidant and a composite antibacterial agent, the nutritional agent is composed of glucose and fructose, the antioxidant is glutathione, and the composite antibacterial agent is composed of penicillin, streptomycin and gentamicin. Accordingly, a corresponding preparation method and a using method are also established. Researches show that the diluted boar semen can survive at 17 DEG C for more than or equal to 144 hours, the activity is more than or equal to 60%, 1 part of the original semen with the sperm density of 200 mL and the sperm density of 1 billion / mL can cover 40 sows, the utilization rate of the original semen is increased by 150%, the breeding cost is remarkably reduced, and the method is suitable for large-scale popularization.

The application discloses a SNP molecular marker related to a semen quality trait gene GNAS, a primer pair and application thereof, and relates to the technical field of pig molecular markers. The pig semen quality trait is semen volume, sperm density, effective sperm density, forward motility, sperm deformity rate and sperm motility; the molecular marker is located in the second intron of the pig GNAS gene, has a nucleotide sequence as shown in the sequence table SEQ ID NO. 1, and a g.277G>A base mutation exists at the 277th position of the sequence. The application further provides a primer pair for amplifying the above-mentioned molecular marker and a method for pig breeding by using the above-mentioned molecular marker. The application first discovers that a SNP molecular marker in the pig GNAS gene is related to the semen volume, sperm density, effective sperm density, forward motility, sperm deformity rate and sperm motility of pigs, and therefore can be used for pig marker-assisted selection breeding, realizes early screening of pig semen quality traits, and the screening method is simple and rapid.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to a pig semen quality trait gene KIF26B, a primer pair and application of the SNP molecular marker and the primer pair, and relates to the technical field of pig molecular markers. The quality traits of the boar semen are semen volume, sperm density, effective sperm density, forward movement activity, sperm malformation rate and sperm motility traits; the molecular marker is located on the upstream of a pig KIF26B gene, the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table, and g.58 T gt exists at the 58bp position of the sequence; and C base mutation. The invention also provides a primer pair for amplifying the molecular marker and a method for breeding pigs by using the molecular marker. The SNP molecular marker in the pig KIF26B gene is found to be related to the semen volume, sperm density, effective sperm density, forward movement activity, sperm malformation rate and sperm activity characters of pigs for the first time, so that the SNP molecular marker can be used for marker-assisted selective breeding of the pigs, early screening of the quality characters of the semen of the pigs is realized, and the screening method is simple and rapid.

The invention discloses an intestinal bacterium for improving semen quality and application thereof, and belongs to the field of microbial technology and biological medicine. In order to solve the problem that in the prior art, specific probiotics are not used for improving male reproductive injury induced by high fat diet, the invention provides a method for applying the allobacter faecalis strain (A.s) or an inactivated form (P-A.s) of the allobacter faecalis strain (A.s) to the male reproductive injury. Animal experiments show that compared with a high fat diet model group, supplementation of A.s or P-A.s can significantly inhibit abnormal weight growth, improve testis index, improve sperm density, vitality, movement speed and testicular testosterone level, and can effectively repair tissue form damage of testis and epididymis and abnormal sperm ultrastructure. Wherein the improvement effect of the inactivated bacterium form P-A.s is particularly prominent.

The Mogroside extract has the effect of improving the damage of the heat stress boar's reproductive capacity. Research shows that, in the process of heat stress of boar, by adding Mogroside extract in the basic diet, the damage of reproductive capacity caused by heat stress can be effectively improved. The test data shows that the Mogroside extract can significantly improve the sperm density, effective sperm number, sperm motility and reduce the sperm abnormality rate of boar; significantly increase the content of a-glucosidase, lactate dehydrogenase, acrosome enzyme and hyaluronidase in the semen, and increase the concentration of testosterone in the serum, enhance the energy supply, structural integrity, functional maturity and fertilization capacity of sperm. Therefore, the Mogroside extract has the potential for further development in the treatment and prevention of heat stress boar's reproductive capacity damage, and can be used for preparing the medicine for improving the heat stress boar's reproductive capacity, so as to further develop the health care function and medicinal value of Siraitia grosvenorii.