Semen product sub-packaging method and sub-packaging quantity determination method and device

Abstract

The invention discloses a semen product subpackaging method and a method and device for determining the subpackaging number, and relates to the technical field of bioscience and semen products, and the method comprises the steps that semen to be subpackaged and semen to be detected are extracted from semen collected for one time of a same male animal; determining the sperm density of the semen to be detected and the haplotype number of the specified gene; determining the number of single sperms based on the determined total number of sperms, the number of haplotypes of the specified gene, the number of single sperms and a relationship among expected probabilities, the expected probabilities representing the probabilities that the semen with the sperm number equal to the number of single sperms contains all different haplotypes of the specified gene; based on the total number of sperms and the number of single sperms, the sub-packaging number of semen products of the semen to be sub-packaged is determined; and sub-packaging the semen to be sub-packaged according to the sub-packaging quantity. By adopting the scheme, the determined sub-packaging quantity of the semen products is more reasonable.

Description

Technical Field

[0001] The present application relates to the fields of biological science and technology and semen product technology, and in particular to a semen product packaging method, a packaging quantity determination method and a device. Background Art

[0002] In artificial assisted reproductive technology, in order to improve the utilization rate of semen, obtain more frozen semen products and achieve economic goals, semen is usually diluted and packaged. In the breeding industry, the dilution multiple of semen packaging is often determined based on the density of sperm in the semen, the amount of semen, the number of bacteria, the number of animals to be inseminated, and the number of sperm per milliliter of semen after dilution.

[0003] However, the traditional method of preparing semen destroys the mathematical distribution characteristics of genetic information in semen under natural conception conditions. For all sexually reproducing species, during natural conception, under specific time and space conditions, the information in the genome of the offspring is affected by the natural selection process.

[0004] There are many types of natural selection, including positive selection, negative selection, balancing selection, etc. The natural selection process determines that the mathematical distribution of allele and genotype frequencies in some regions of the offspring's individual genome is non-random. This non-random distribution is essentially the probability that different sperm and eggs of the same individual will successfully combine and develop into healthy individuals during the natural conception process. It is not evenly distributed according to the number of germ cells.

[0005] During meiosis in sexually reproducing species, gene recombination between sister chromatids results in very high genetic polymorphism between the genomes of different germ cells in the gonads. The rich genetic polymorphism of sperm or eggs provides the basis for the optimal combination of genomic information during each sperm-egg combination. This non-random distribution of genomic information has an important impact on the individual's environmental adaptability, disease resistance, and the expression of economic traits.

[0006] In addition to the mathematical distribution of sperm genetic information, sperm density is also an important factor affecting the success rate of reproduction. A higher sperm density can increase the chance of sperm and egg meeting and improve the probability of fertilization. In addition, a higher density can also increase the distribution of sperm in the uterus and increase the possibility of conception. However, too high a density may also lead to too fierce competition among sperm, making some sperm unable to successfully fertilize.

[0007] At present, in the breeding industry, due to the high purchase price and breeding costs of breeding bulls, in order to improve the utilization rate of breeding bulls, in addition to the breeding bull semen products sold to the outside world being subject to national laws and regulations, in the implementation of artificial assisted reproductive technology, as long as the female animal can conceive, the packaging and dilution of semen products often involves excessive dilution.

[0008] The dilution multiple of semen is often mechanically based on fixed empirical data. The control of the dilution multiple does not take into account the impact of the degree of packaging on the level of genomic genetic polymorphism in the semen stock. The genetic polymorphism of semen decreases after dilution and packaging, which greatly reduces the probability of achieving optimal combination of genomic information of the fertilized egg in the subsequent sperm-egg combination process. This non-optimal combination of fertilization often manifests itself in decreased production performance or susceptibility to disease in the subsequent breeding process.

[0009] The sperm density of breeding animals is not a stable indicator. The sperm density of the same male animal varies with age. The sperm density of different breeds and individuals is also different. However, in practice, the semen dilution multiples are often not adjusted in a targeted manner.

[0010] Similar situations also exist in the artificial breeding process of rare animals. Rare animals objectively have insufficient population content and few male animals. They are prone to inbreeding-induced decline under natural conception. Moreover, the sperm density of different species has different effects on the success rate of reproduction. There is a lack of quantitative basis on how to ensure the success rate of reproduction and optimize the use of semen from male animals.

[0011] In summary, if the semen dilution multiple is too high, that is, the number of semen packages for a certain volume is too large, the genetic polymorphism of the semen will decrease. If the number of semen packages is too low, it will lead to waste of semen and low utilization rate. That is, there is a problem of unreasonable number of semen product packages in the prior art. Summary of the invention

[0012] The embodiments of the present application provide a method for packaging a semen product, a method for determining the packaging quantity, and a device, so as to solve the problem of unreasonable packaging quantity of semen products existing in the prior art.

[0013] The present application embodiment provides a method for packaging a semen product, comprising:

[0014] Extracting semen to be packaged and semen to be tested from semen collected from the same male animal in a single time;

[0015] Determining the sperm density and the haplotype number of the specified gene of the semen to be tested;

[0016] Calculating the total number of sperm in the semen to be packaged based on the sperm density and semen volume of the semen to be packaged;

[0017] Determine the number of single sperm based on the total number of sperm, the number of haplotypes of the specified gene and the number of single sperm, and the expected probability, wherein the expected probability represents the probability that semen with the number of sperms of the single sperm number contains all different haplotypes of the specified gene;

[0018] Based on the total number of spermatozoa and the number of spermatozoa in a single sperm, determining the number of semen products to be packaged;

[0019] The semen to be packaged is packaged according to the packaging quantity.

[0020] Furthermore, the method of extracting the semen to be packaged and the semen to be tested from the semen collected from the same male animal at a single time includes:

[0021] Extracting a first preset volume of semen to be packaged from semen collected from the same male animal once, and storing the semen in cold storage;

[0022] A second preset volume of semen to be tested is extracted from semen collected once from the same male animal.

[0023] Furthermore, the haplotype number and sperm density of the designated gene of the semen to be tested are determined, including:

[0024] Separating the genome of the semen to be tested, and determining the haplotype number of the designated gene;

[0025] The sperm density of the semen to be tested is measured using a sperm quality analyzer.

[0026] Furthermore, the relationship between the total sperm count, the haplotype number of the specified gene and the number of individual sperm, and the expected probability satisfies the following relationship:

[0027]

[0028] Wherein, N represents the number of haplotypes of the specified gene, S represents the total number of sperm, X represents the number of single sperm, and P represents the expected probability.

[0029] Further, the determining of the number of individual sperm based on the total number of sperm, the number of haplotypes of the specified gene and the number of individual sperm, and the relationship between the expected probability, includes:

[0030] The minimum number of sperm in a single sperm is calculated using the following formula:

[0031]

[0032] Among them, X min is the minimum number of sperm in a single sperm;

[0033] X min Round up to get the number of sperm in a single sperm.

[0034] Further, based on the total number of spermatozoa and the number of spermatozoa in a single sperm, determining the number of semen products to be packaged includes:

[0035] Calculate the total number of spermatozoa divided by the number of spermatozoa in a single tube, and round the quotient to obtain the number of semen products to be packaged; or

[0036] The total number of spermatozoa is calculated by dividing the total number of spermatozoa by the number of spermatozoa in a single tube and then multiplying the result by the corrected probability, and the result is rounded to obtain the number of semen products to be packaged. The corrected probability is one of the following probabilities, or the product of at least two of the following probabilities:

[0037] It indicates the percentage of sperm moving in a straight line after thawing of semen;

[0038] Sperm recovery rate;

[0039] Sperm motility of fresh semen.

[0040] Furthermore, the designated gene is the growth hormone GH gene, the peroxisome proliferator-activated receptor γ PPARG gene or the calpain 3 GAPN3 gene.

[0041] The present application also provides a method for determining the number of semen product packaging, including:

[0042] Obtain the haplotype number of the specified gene, sperm density and semen volume of the semen to be packaged;

[0043] Calculating the total number of sperm in the semen to be packaged based on the sperm density and the semen volume;

[0044] Determine the number of single sperm based on the total number of sperm, the number of haplotypes of the specified gene and the number of single sperm, and the expected probability, wherein the expected probability represents the probability that semen with the number of sperms of the single sperm number contains all different haplotypes of the specified gene;

[0045] Based on the total number of sperms and the number of sperms in each sperm, the number of semen products to be packaged is determined.

[0046] Furthermore, the relationship between the total sperm count, the haplotype number of the specified gene and the number of individual sperm, and the expected probability satisfies the following relationship:

[0047]

[0048] Wherein, N represents the number of haplotypes of the specified gene, S represents the total number of sperm, X represents the number of single sperm, and P represents the expected probability.

[0049] Further, the determining of the number of individual sperm based on the total number of sperm, the number of haplotypes of the specified gene and the number of individual sperm, and the relationship between the expected probability, includes:

[0050] The minimum number of sperm in a single sperm is calculated using the following formula:

[0051]

[0052] Among them, X min is the minimum number of sperm in a single sperm;

[0053] X min Round up to get the number of sperm in a single sperm.

[0054] Further, based on the total number of spermatozoa and the number of spermatozoa in a single sperm, determining the number of semen products to be packaged includes:

[0055] Calculate the total number of spermatozoa divided by the number of spermatozoa in a single tube, and round the quotient to obtain the number of semen products to be packaged; or

[0056] The total number of spermatozoa is calculated by dividing the total number of spermatozoa by the number of spermatozoa in a single tube and then multiplying the result by the corrected probability, and the result is rounded to obtain the number of semen products to be packaged. The corrected probability is one of the following probabilities, or the product of at least two of the following probabilities:

[0057] It indicates the percentage of sperm moving in a straight line after thawing of semen;

[0058] Sperm recovery rate;

[0059] Sperm motility of fresh semen.

[0060] Furthermore, the designated gene is the growth hormone GH gene, the peroxisome proliferator-activated receptor γ PPARG gene or the calpain 3 GAPN3 gene.

[0061] The present application also provides a device for determining the number of semen product packaging, including:

[0062] An indicator acquisition module is used to obtain the haplotype number of a specified gene, sperm density and semen volume of the semen to be packaged;

[0063] A total sperm count calculation module, used for calculating the total sperm count of the semen to be packaged based on the sperm density and the semen volume;

[0064] A single sperm number determination module, used to determine the single sperm number based on the total sperm number, the haplotype number of the specified gene and the single sperm number, and the relationship between the expected probability, wherein the expected probability represents the probability that the semen with the sperm number of the single sperm number contains all different haplotypes of the specified gene;

[0065] The module for determining the number of packages is used to determine the number of packages of the semen product to be packaged based on the total number of sperm and the number of sperm in a single sperm.

[0066] Furthermore, the relationship between the total sperm count, the haplotype number of the specified gene and the number of individual sperm, and the expected probability satisfies the following relationship:

[0067]

[0068] Wherein, N represents the number of haplotypes of the specified gene, S represents the total number of sperm, X represents the number of single sperm, and P represents the expected probability.

[0069] Furthermore, the single sperm number determination module is specifically used to calculate the minimum value of the single sperm number using the following formula:

[0070]

[0071] Among them, X min is the minimum number of sperm in a single sperm;

[0072] X min Round up to get the number of sperm in a single sperm.

[0073] Furthermore, the packaging quantity determination module is specifically used to calculate the total number of sperm divided by the number of sperm in a single tube, and to round the obtained quotient to obtain the packaging quantity of the semen product to be packaged; or

[0074] The total number of spermatozoa is calculated by dividing the total number of spermatozoa by the number of spermatozoa in a single tube and then multiplying the result by the corrected probability, and the result is rounded to obtain the number of semen products to be packaged. The corrected probability is one of the following probabilities, or the product of at least two of the following probabilities:

[0075] It indicates the percentage of sperm moving in a straight line after thawing of semen;

[0076] Sperm recovery rate;

[0077] Sperm motility of fresh semen.

[0078] Furthermore, the designated gene is the growth hormone GH gene, the peroxisome proliferator-activated receptor γ PPARG gene or the calpain 3 GAPN3 gene.

[0079] An embodiment of the present application also provides an electronic device, including a processor and a machine-readable storage medium, wherein the machine-readable storage medium stores machine-executable instructions that can be executed by the processor, and the processor is prompted by the machine-executable instructions to implement any of the above-mentioned methods for determining the number of semen product packages.

[0080] An embodiment of the present application also provides a computer-readable storage medium, characterized in that a computer program is stored in the computer-readable storage medium, and when the computer program is executed by a processor, it implements any of the above-mentioned methods for determining the packaging quantity of semen products.

[0081] An embodiment of the present application also provides a computer program product comprising instructions, which, when executed on a computer, enables the computer to execute any of the above-mentioned methods for determining the number of semen product packages.

[0082] The beneficial effects of this application include:

[0083] In the method provided in the embodiment of the present application, the total number of sperm in the semen to be packaged is calculated based on the sperm density and semen volume of the semen to be packaged, and the number of sperm in a single sperm is determined based on the total number of sperm, the number of haplotypes of a specified gene and the number of sperm in a single sperm, and the relationship between the expected probability, wherein the expected probability represents the probability that a semen with a sperm number equal to the number of sperm in a single sperm contains all different haplotypes of the specified gene, and then the number of semen products to be packaged of the semen to be packaged is determined based on the total number of sperm and the number of sperm in a single sperm. That is to say, in the process of determining the number of semen products to be packaged , taking into account the relationship between sperm density, the number of haplotypes of a specified gene and the number of a single sperm, and the probability that the semen contains all different haplotypes of the specified gene. Therefore, the number of a single sperm determined based on this relationship can reach the expected probability, and the number of haplotypes of a specified gene can represent the genetic polymorphism of semen, thereby making the number of semen products packaged based on the number of a single sperm more reasonable, that is, the semen products packaged according to the packaging number can meet the requirements of semen genetic polymorphism, reduce semen waste, and improve semen utilization.

[0084] Other features and advantages of the present application will be described in the following description, and partly become apparent from the description, or be understood by practicing the present application. The purpose and other advantages of the present application can be realized and obtained by the structures specifically pointed out in the written description, claims, and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0085] The accompanying drawings are used to provide a further understanding of the present application and constitute a part of the specification. Together with the embodiments of the present application, they are used to explain the present application and do not constitute a limitation of the present application. In the accompanying drawings:

[0086] Figure 1 A flow chart of a method for packaging semen products provided in an embodiment of the present application;

[0087] Figure 2 A flow chart of a method for packaging a semen product provided in another embodiment of the present application;

[0088] Figure 3 A flow chart of a method for determining the number of semen product packages provided in an embodiment of the present application;

[0089] Figure 4 A schematic diagram of the structure of a device for determining the number of semen product packages provided in an embodiment of the present application;

[0090] Figure 5 A schematic diagram of the structure of an electronic device provided in an embodiment of the present application. DETAILED DESCRIPTION

[0091] In order to provide a solution for determining a more reasonable number of semen product packaging, the embodiments of the present application provide a method for packaging semen products, a method for determining the number of packaging, and a device. The preferred embodiments of the present application are described below in conjunction with the drawings of the specification. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present application, and are not used to limit the present application. In addition, the embodiments of the present application and the features in the embodiments can be combined with each other if there is no conflict.

[0092] The present application embodiment provides a method for packaging a semen product, such as Figure 1 As shown, including:

[0093] Step 11, extracting semen to be packaged and semen to be tested from the semen collected once from the same male animal;

[0094] Step 12, measuring the sperm density of the semen to be tested and the haplotype number of the designated gene;

[0095] Step 13, calculating the total number of sperm in the semen to be packaged based on the sperm density and semen volume of the semen to be packaged;

[0096] Step 14, determining the number of single sperm based on the total number of sperm, the number of haplotypes of the specified gene and the number of single sperm, and the expected probability, wherein the expected probability represents the probability that a semen having the number of sperm of the single sperm contains all different haplotypes of the specified gene;

[0097] Step 15: Determine the number of semen products to be packaged based on the total number of sperm and the number of sperm in a single sperm;

[0098] Step 16: Pack the semen according to the packing quantity.

[0099] The above-mentioned semen product packaging method provided in the embodiment of the present application is adopted to calculate the total number of sperms in the semen to be packaged based on the sperm density and semen volume of the semen to be packaged, and determine the number of single sperms based on the total number of sperms, the number of haplotypes of the specified gene and the number of single sperms, and the relationship between the expected probability, wherein the expected probability represents the probability that the semen with the number of sperms of the number of single sperms contains all different haplotypes of the specified gene, and then determine the number of semen products to be packaged based on the total number of sperms and the number of single sperms. That is to say, in determining the number of semen products to be packaged, In the process, the relationship between sperm density, the number of haplotypes of the specified gene and the number of single sperm, and the probability of all different haplotypes of the specified gene being contained in the semen are taken into consideration. Therefore, the number of single sperm determined based on the relationship can reach the expected probability, and the number of haplotypes of the specified gene can represent the genetic polymorphism of semen, thereby making the number of semen products packaged based on the number of single sperm more reasonable, that is, the semen products packaged according to the packaging number can meet the requirements of semen genetic polymorphism, reduce semen waste, and improve semen utilization.

[0100] The semen product packaging method provided by the present application is described in detail below with reference to the accompanying drawings using specific embodiments.

[0101] The present application embodiment provides a method for packaging a semen product, such as Figure 2 As shown, including:

[0102] Step 21: extract a first preset volume of semen to be packaged from the semen collected from the male animal once, and store it in a cold storage.

[0103] In practical applications, semen can be collected from various male animals as semen to be packaged, and the semen product packaging method provided in the embodiments of the present application can be implemented.

[0104] The volume of the semen to be packaged, that is, the first preset volume, can be flexibly set based on the needs of actual application.

[0105] For example, when collecting semen from Angus bulls, a 5 ml volume of semen can be extracted as the semen to be packaged and stored in a refrigerator.

[0106] Step 22: extracting a second preset volume of semen to be tested from the semen collected once from the same male animal.

[0107] In this step, the semen to be tested is used for subsequent determination of various indicators of the semen, and the second preset volume can be flexibly set based on actual application and testing needs.

[0108] For example, 200 microliters of semen can be extracted from semen collected from the same male animal at a single time as the semen to be tested.

[0109] Step 23: Separate the genome of the semen to be tested and measure the haplotype number of the designated gene.

[0110] In this step, the haplotype number of various designated genes can be determined based on the needs of practical applications. For example, the designated gene can be the GH (Growth Hormone) gene, the PPARG (Peroxisome Proliferative Activated Receptor gamma) gene or the GAPN3 (Calpain 3) gene.

[0111] In this step, taking the GH gene as an example, the genome of the semen to be tested is separated and used for sequencing. The sequencing depth can be 50X. The sequencing depth can be selected as long as the haplotype diversity does not increase with the sequencing depth.

[0112] In the experiment, the haplotype number of the GH gene in the semen of Angus bulls was determined to be 102 by second-generation sequencing.

[0113] In the embodiments of the present application, the haplotype number of the specified gene is used as a genetic polymorphism indicator to represent the genetic polymorphism of semen. In practical applications, other genetic polymorphism indicators can also be used to represent the genetic polymorphism of semen, for example, nucleotide diversity, effective population content and other indicators.

[0114] Step 24: Use a sperm quality analyzer to measure the sperm density of the semen to be tested.

[0115] Based on the need to determine the number of subpackaging items later, the sperm motility of the semen to be tested can also be measured in this step.

[0116] In the experiment, this step was performed on the semen of Angus bulls, and the results were as follows:

[0117] The sperm density was 1.056 billion / ml and the sperm motility was 82.16%.

[0118] Step 25: Calculate the total number of sperm in the semen to be packaged based on the sperm density and semen volume of the semen to be packaged.

[0119] Taking the sperm density of 1.056 billion / ml and the semen volume of 5 ml as an example, the total number of sperm in the semen to be packaged is 1.056 times 5, which equals 5.28 billion.

[0120] Step 26: Determine the number of sperm per sperm based on the total number of sperm in the semen to be packaged, the number of haplotypes of the specified gene and the number of sperm per sperm, and the expected probability, wherein the expected probability represents the probability that the semen with the number of sperm per sperm contains all different haplotypes of the specified gene.

[0121] In this step, based on the above relationship, the number of individual sperm can be determined when the total number of sperm, the number of haplotypes of a specified gene, and the expected probability are known.

[0122] Specifically, in the embodiments of the present application, the relationship between the total number of sperm, the number of haplotypes of a specified gene and the number of sperm in a single sperm, and the expected probability is proposed to satisfy the following relationship:

[0123]

[0124] Among them, N represents the number of haplotypes of the specified gene, S represents the total number of sperm, X represents the number of individual sperm, and P represents the expected probability.

[0125] It can be seen from the above relationship that when the expected probability P remains unchanged, the larger the number of single sperm X is, the greater the possibility of satisfying the above relationship. Therefore, based on the above relationship, the minimum value of the number of single sperm X that satisfies the known expected probability P can be calculated.

[0126] Specifically, in this step, the minimum number of sperm in a single sperm can be calculated using the following formula:

[0127]

[0128] Among them, X min It is the minimum number of sperm in a single sperm;

[0129] X min Round up and the result can be used as the number of sperm in a single sperm.

[0130] In practical applications, the expected probability can be flexibly set based on needs.

[0131] In an example, taking the expected probability as 98%, the sperm density as 1.056 billion / ml, the semen volume to be packaged as 5 ml, and the haplotype number of the GH gene as 102 as an example, the above calculation method of this step is used to obtain a calculation result of 1985341 sperm count per sperm.

[0132] The derivation process of the above relationship is described as follows:

[0133] S represents the total number of sperm, and N represents the number of haplotypes of a specified gene. The probability Pa of a semen product randomly packaged from the semen to be packaged containing N different haplotypes of a specified gene is as follows:

[0134] P a =N / S;

[0135] The probability Pb ​​that the randomly packaged semen product does not contain N different haplotypes of the specified gene is as follows:

[0136] P b =1-P a =1-N / S;

[0137] Assuming that the number of sperm contained in the randomly packaged semen product is X, the probability Pc that the semen product does not contain any haplotype of the specified gene is as follows:

[0138]

[0139] The probability Pd that the randomly packaged semen product contains at least one haplotype of the specified gene is as follows:

[0140]

[0141] The probability Pe that a randomly divided semen product contains all the different haplotypes of a given gene is as follows:

[0142]

[0143] Need to meet P e >P, that is, we get the above relationship

[0144] Step 27: Based on the total number of spermatozoa and the number of spermatozoa in a single sperm tube, determine the number of semen products to be packaged.

[0145] In this step, the quantity of semen product packaging can be determined in the following manner:

[0146] The first method is to calculate the total number of spermatozoa divided by the number of spermatozoa in a single tube, and then round the quotient to the nearest integer to obtain the number of semen products to be packaged.

[0147] For example, in the above example, the calculated number of sperm in a single tube is 1985341, and the total number of sperm in the semen to be packaged is 5.28 billion. Using the first method to calculate 5280000000 / 1985341=2659.5, the quotient is rounded to 2659, that is, the number of semen products to be packaged is 2659.

[0148] The second method is to calculate the total number of sperm divided by the number of sperm in a single tube and then multiply by the corrected probability, and round the result to get the number of semen products to be packaged. The corrected probability is less than 1.

[0149] Taking into account that in actual applications, not all sperm in the packaged semen products may survive or function, the quotient obtained by dividing the total number of sperm by the number of sperm in a single tube can be corrected, that is, multiplied by the corrected probability, and the corrected probability is less than 1, thereby reducing the number of packages and increasing the number of sperm contained in a single semen product, which makes it more likely to meet the above expected probability.

[0150] The second method can also be understood as correcting the number of individual sperms with the corrected probability, that is, first calculating the number of individual sperms divided by the corrected probability to get the quotient, and then calculating the total number of sperms divided by the quotient.

[0151] The correction probability can be set based on experimental data or empirical values.

[0152] Furthermore, in the embodiment of the present application, it is proposed that the modified probability may be one of the following probabilities, or the product of at least two of the following probabilities:

[0153] It indicates the percentage of sperm moving in a straight line after thawing of semen;

[0154] Sperm recovery rate;

[0155] Sperm motility of fresh semen.

[0156] For example, in the above example, the calculated number of single sperm is 1985341, the total number of sperm in the semen to be packaged is 5.28 billion, and the corrected probability is the product of the above three probabilities, where the percentage of sperm moving in a straight line after thawing is 30% (lower limit), the sperm recovery rate (lower limit) is 50%, and the sperm motility is 82.16%. The calculation process is as follows:

[0157] (5280000000 / 1985341)*30%*50%*82.16%=327.75;

[0158] The rounded result is 327.

[0159] Step 28: Pack the semen according to the calculated packing quantity.

[0160] For example, in the above example, the semen to be packaged is packaged according to the calculated packaging quantity 327, that is, the semen to be packaged is evenly divided into 327 portions, and each portion is a semen product.

[0161] With respect to the above example, in the experiment, several semen products were randomly selected, and the haplotype number of the GH gene of each semen product was measured, and the result was 102, indicating that the above method provided in the embodiment of the present application is effective in ensuring the genetic polymorphism of semen products.

[0162] In the above-mentioned semen product packaging method provided in the embodiment of the present application, the correlation between sperm density, sperm count, semen volume, packaging quantity and genetic polymorphism indicators is proposed, that is, the problem of quantitative correlation between various indicators is solved.

[0163] Thereby, the determined packaging quantity of semen products is more reasonable, and the semen products obtained by packaging according to the packaging quantity can meet the requirements of semen genetic polymorphism, reduce semen waste, and improve semen utilization.

[0164] It further solves the problem of decreased genetic polymorphism in traditional semen products and the problem of optimal utilization of male individuals.

[0165] The present application also provides a method for determining the number of semen product packages. Figure 3 As shown, including:

[0166] Step 31, obtaining the haplotype number of the designated gene, sperm density and semen volume of the semen to be packaged;

[0167] Step 32: Calculate the total number of sperm in the semen to be packaged based on the sperm density and the semen volume;

[0168] Step 33, determining the number of single sperm based on the total number of sperm, the number of haplotypes of the specified gene and the number of single sperm, and the expected probability, wherein the expected probability represents the probability that a semen having the number of sperm of the single sperm contains all different haplotypes of the specified gene;

[0169] Step 34: Based on the total number of spermatozoa and the number of spermatozoa in a single sperm tube, determine the number of semen products to be packaged.

[0170] In the above method provided in the embodiment of the present application, the total number of sperm in the semen to be packaged is calculated based on the sperm density and semen volume of the semen to be packaged, and the number of sperm in a single sperm is determined based on the total number of sperm, the number of haplotypes of a specified gene and the number of sperm in a single sperm, and the relationship between the expected probability, wherein the expected probability represents the probability that a semen with a sperm number equal to the number of sperm in a single sperm contains all different haplotypes of the specified gene, and then the number of semen products to be packaged of the semen to be packaged is determined based on the total number of sperm and the number of sperm in a single sperm. That is to say, in the process of determining the number of semen products to be packaged In the method, the relationship between sperm density, the number of haplotypes of a specified gene, the number of a single sperm, and the probability of containing all different haplotypes of a specified gene in semen is taken into account. Therefore, the number of a single sperm determined based on the relationship can reach the expected probability, and the number of haplotypes of a specified gene can represent the genetic polymorphism of semen, thereby making the number of semen products packaged based on the number of a single sperm more reasonable, that is, the semen products packaged according to the packaging number can meet the requirements of semen genetic polymorphism, reduce semen waste, and improve semen utilization.

[0171] In the embodiment of the present application, steps 32 to 34 of the method for determining the number of semen product packages can specifically adopt the same calculation process as the above steps 25 to 27, and will not be described in detail here.

[0172] Based on the same inventive concept, according to the method for determining the number of semen product packaging provided in the above embodiment of the present application, correspondingly, another embodiment of the present application also provides a device for determining the number of semen product packaging, and its structural schematic diagram is shown as follows: Figure 4 As shown, specifically including:

[0173] An indicator acquisition module 41 is used to obtain the haplotype number of a specified gene of the semen to be packaged, sperm density and semen volume;

[0174] A total sperm count calculation module 42, used to calculate the total sperm count of the semen to be packaged based on the sperm density and the semen volume;

[0175] A single sperm number determination module 43 is used to determine the single sperm number based on the total sperm number, the haplotype number of the specified gene and the single sperm number, and the relationship between the expected probability, wherein the expected probability represents the probability that the semen with the sperm number of the single sperm number contains all different haplotypes of the specified gene;

[0176] The packaging quantity determination module 44 is used to determine the packaging quantity of the semen product to be packaged based on the total number of sperm and the number of sperm in a single sperm.

[0177] Furthermore, the relationship between the total sperm count, the haplotype number of the specified gene and the number of individual sperm, and the expected probability satisfies the following relationship:

[0178]

[0179] Wherein, N represents the number of haplotypes of the specified gene, S represents the total number of sperm, X represents the number of single sperm, and P represents the expected probability.

[0180] Furthermore, the single sperm number determination module 43 is specifically used to calculate the minimum value of the single sperm number using the following formula:

[0181]

[0182] Among them, X min is the minimum number of sperm in a single sperm;

[0183] X min Round up to get the number of sperm in a single sperm.

[0184] Furthermore, the packaging quantity determination module 44 is specifically used to calculate the total number of sperm divided by the number of sperm in a single tube, and round the obtained quotient to obtain the packaging quantity of the semen product to be packaged; or

[0185] The total number of spermatozoa is calculated by dividing the total number of spermatozoa by the number of spermatozoa in a single tube and then multiplying the result by the corrected probability, and the result is rounded to obtain the number of semen products to be packaged. The corrected probability is one of the following probabilities, or the product of at least two of the following probabilities:

[0186] It indicates the percentage of sperm moving in a straight line after thawing of semen;

[0187] Sperm recovery rate;

[0188] Sperm motility of fresh semen.

[0189] Furthermore, the designated gene is the growth hormone GH gene, the peroxisome proliferator-activated receptor γ PPARG gene or the calpain 3 GAPN3 gene.

[0190] The functions of the above modules can correspond to Figures 1 to 3 The corresponding processing steps in the shown process will not be repeated here.

[0191] The device for determining the number of semen product packaging provided in the embodiment of the present application can be implemented by a computer program. Those skilled in the art should be able to understand that the above-mentioned module division method is only one of many module division methods. If it is divided into other modules or no modules, as long as the device for determining the number of semen product packaging has the above-mentioned functions, it should be within the scope of protection of the present application.

[0192] The present application also provides an electronic device, such as Figure 5 As shown, it includes a processor 51 and a machine-readable storage medium 52, wherein the machine-readable storage medium 52 stores machine-executable instructions that can be executed by the processor 51, and the processor 51 is prompted by the machine-executable instructions to implement any of the above-mentioned methods for determining the packaging quantity of semen products.

[0193] An embodiment of the present application also provides a computer-readable storage medium, characterized in that a computer program is stored in the computer-readable storage medium, and when the computer program is executed by a processor, it implements any of the above-mentioned methods for determining the packaging quantity of semen products.

[0194] An embodiment of the present application also provides a computer program product comprising instructions, which, when executed on a computer, enables the computer to execute any of the above-mentioned methods for determining the number of semen product packages.

[0195] The machine-readable storage medium in the electronic device may include a random access memory (RAM) or a non-volatile memory (NVM), such as at least one disk memory. Optionally, the memory may also be at least one storage device located away from the processor.

[0196] The above-mentioned processor can be a general-purpose processor, including a central processing unit (CPU), a network processor (NP), etc.; it can also be a digital signal processor (DSP), an application specific integrated circuit (ASIC), a field programmable gate array (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components.

[0197] Each embodiment in this specification is described in a related manner, and the same or similar parts between the embodiments can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, for the device, electronic device, computer-readable storage medium, and computer program product embodiments, since they are basically similar to the method embodiments, the description is relatively simple, and the relevant parts can be referred to the partial description of the method embodiments.

[0198] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0199] The present application is described with reference to the flowcharts and / or block diagrams of the methods, devices (systems), and computer program products according to the embodiments of the present application. It should be understood that each process and / or box in the flowchart and / or block diagram, as well as the combination of the processes and / or boxes in the flowchart and / or block diagram, can be implemented by computer program instructions. These computer program instructions can be provided to a processor of a general-purpose computer, a special-purpose computer, an embedded processor, or other programmable data processing device to generate a machine, so that the instructions executed by the processor of the computer or other programmable data processing device generate instructions for implementing the processes in the flowchart and / or block diagram. Figure 1 A process or multiple processes and / or boxes Figure 1 A device that provides the functions specified in a block or multiple blocks.

[0200] These computer program instructions may also be stored in a computer-readable memory capable of directing a computer or other programmable data processing device to operate in a specific manner, so that the instructions stored in the computer-readable memory produce an article of manufacture including an instruction device, which implements the process Figure 1 A process or multiple processes and / or boxes Figure 1 A function specified in one or more boxes.

[0201] These computer program instructions can also be loaded onto a computer or other programmable data processing device so that a series of operating steps are executed on the computer or other programmable device to produce a computer-implemented process, thereby providing instructions for implementing the process in the computer or other programmable device. Figure 1 A process or multiple processes and / or boxes Figure 1 The steps for the functions specified in one or more boxes.

[0202] Obviously, those skilled in the art can make various changes and modifications to the present application without departing from the spirit and scope of the present application. Thus, if these modifications and variations of the present application fall within the scope of the claims of the present application and their equivalents, the present application is also intended to include these modifications and variations.

Claims

1. A method for packaging a semen product, characterized in that: include: Extracting semen to be packaged and semen to be tested from semen collected from the same male animal in a single time; Determining the sperm density and the haplotype number of the specified gene of the semen to be tested; Calculating the total number of sperm in the semen to be packaged based on the sperm density and semen volume of the semen to be packaged; Determine the number of single sperm based on the total number of sperm, the number of haplotypes of the specified gene and the number of single sperm, and the expected probability, wherein the expected probability represents the probability that semen with the number of sperms of the single sperm number contains all different haplotypes of the specified gene; Based on the total number of spermatozoa and the number of spermatozoa in a single sperm, determining the number of semen products to be packaged; The semen to be packaged is packaged according to the packaging quantity.

2. The method according to claim 1, characterized in that The method of extracting semen to be packaged and semen to be tested from semen collected from the same male animal at a single time comprises: Extracting a first preset volume of semen to be packaged from semen collected from the same male animal once, and storing the semen in cold storage; A second preset volume of semen to be tested is extracted from semen collected once from the same male animal.

3. The method according to claim 1, characterized in that The method of measuring the sperm density of the semen to be tested and the haplotype number of the specified gene comprises: Separating the genome of the semen to be tested, and determining the haplotype number of the designated gene; The sperm density of the semen to be tested is measured using a sperm quality analyzer.

4. The method according to claim 1, characterized in that The relationship between the total sperm count, the haplotype number of the specified gene and the number of individual sperm, and the expected probability satisfies the following relationship: Wherein, N represents the number of haplotypes of the specified gene, S represents the total number of sperm, X represents the number of single sperm, and P represents the expected probability.

5. The method according to claim 4, characterized in that Determining the number of individual sperm based on the total number of sperm, the number of haplotypes of the specified gene and the number of individual sperm, and the relationship between the expected probability, comprises: The minimum number of sperm in a single sperm is calculated using the following formula: Among them, X min is the minimum number of sperm in a single sperm; X min Round up to get the number of sperm in a single sperm.

6. The method according to claim 1, characterized in that Determining the number of semen products to be packaged based on the total number of sperm and the number of sperm in a single sperm, comprises: Calculate the total number of spermatozoa divided by the number of spermatozoa in a single tube, and round the quotient to obtain the number of semen products to be packaged; or The total number of spermatozoa is calculated by dividing the total number of spermatozoa by the number of spermatozoa in a single tube and then multiplying the result by the corrected probability, and the result is rounded to obtain the number of semen products to be packaged. The corrected probability is one of the following probabilities, or the product of at least two of the following probabilities: It indicates the percentage of sperm moving in a straight line after thawing of semen; Sperm recovery rate; Sperm motility of fresh semen.

7. The method according to any one of claims 1 to 6, characterized in that: The designated gene is the growth hormone GH gene, the peroxisome proliferator-activated receptor γ PPARG gene or the calpain 3 GAPN3 gene.

8. A method for determining the number of packages of a semen product, characterized in that: include: Obtain the haplotype number of the specified gene, sperm density and semen volume of the semen to be packaged; Calculating the total number of sperm in the semen to be packaged based on the sperm density and the semen volume; Determine the number of single sperm based on the total number of sperm, the number of haplotypes of the specified gene and the number of single sperm, and the expected probability, wherein the expected probability represents the probability that semen with the number of sperms of the single sperm number contains all different haplotypes of the specified gene; Based on the total number of sperms and the number of sperms in each sperm, the number of semen products to be packaged is determined.

9. The method according to claim 8, characterized in that The relationship between the total sperm count, the haplotype number of the specified gene and the number of individual sperm, and the expected probability satisfies the following relationship: Wherein, N represents the number of haplotypes of the specified gene, S represents the total number of sperm, X represents the number of single sperm, and P represents the expected probability.

10. The method according to claim 9, characterized in that Determining the number of individual sperm based on the total number of sperm, the number of haplotypes of the specified gene and the number of individual sperm, and the relationship between the expected probability, comprises: The minimum number of sperm in a single sperm is calculated using the following formula: Among them, X min is the minimum number of sperm in a single sperm; X min Round up to get the number of sperm in a single sperm.

11. The method according to claim 8, characterized in that Determining the number of semen products to be packaged based on the total number of sperm and the number of sperm in a single sperm, comprises: Calculate the total number of spermatozoa divided by the number of spermatozoa in a single tube, and round the quotient to obtain the number of semen products to be packaged; or The total number of spermatozoa is calculated by dividing the total number of spermatozoa by the number of spermatozoa in a single tube and then multiplying the result by the corrected probability, and the result is rounded to obtain the number of semen products to be packaged. The corrected probability is one of the following probabilities, or the product of at least two of the following probabilities: It indicates the percentage of sperm moving in a straight line after thawing of semen; Sperm recovery rate; Sperm motility of fresh semen.

12. The method according to any one of claims 8 to 11, characterized in that: The designated gene is the growth hormone GH gene, the peroxisome proliferator-activated receptor γ PPARG gene or the calpain 3 GAPN3 gene.

13. A device for determining the quantity of semen product packaging, characterized in that: include: An indicator acquisition module is used to obtain the haplotype number of a specified gene, sperm density and semen volume of the semen to be packaged; A total sperm count calculation module, used for calculating the total sperm count of the semen to be packaged based on the sperm density and the semen volume; A single sperm number determination module, used to determine the single sperm number based on the total sperm number, the haplotype number of the specified gene and the single sperm number, and the relationship between the expected probability, wherein the expected probability represents the probability that the semen with the sperm number of the single sperm number contains all different haplotypes of the specified gene; The module for determining the number of packages is used to determine the number of packages of the semen product to be packaged based on the total number of sperm and the number of sperm in a single sperm.

14. An electronic device, characterized in that: The method comprises a processor and a machine-readable storage medium, wherein the machine-readable storage medium stores machine-executable instructions that can be executed by the processor, and the processor is prompted by the machine-executable instructions to implement the method described in any one of claims 8 to 12.

15. A computer-readable storage medium, characterized in that: The computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the method according to any one of claims 8 to 12 is implemented.

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