80 results about "Sperm Collection" patented technology

The invention relates to Chinese herbal medicine feed additive for stud rams, which mainly solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art. The Chinese herbal medicine feed additive is prepared by crashing and mixing epimedium, actinolite, Chinese dodder seed, raspberry, Chinese magnoliavine fruit, astragalus root and dangshen and can be added into feed for usage. The invention improves ram sexual appetite, prompts spermatogenesis, increases sperm motility, effectively improves reproductive performance, solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art according to spermatogenesis principle.

The present invention is a sperm collector with squeezing function. It includes a soft rubber body provided with a penis insertion passage and a container body provided with at least a top end cover and receives the soft rubber body. When the top end cover and the container body are engaged, the soft rubber body is confined within a cavity formed by the top end cover and the container body. When the top end cover is disengaged, a front end portion of the soft rubber body protrudes out of an opening of the container body. A pressing means is provided at a middle lower portion of the container body. When using the present invention, the user could press the pressing means to squeeze the penis insertion passage of the sperm collector. Accordingly, stimulation to penis is increased and better effect of sperm collection is achieved. In particular, since the present invention is also provided with a heating rod, lubricant pockets and electrical vibrators, which enable the user to reach a climax as quick as possible, thereby further enhancing the effect of sperm collection.

A sperm collection device includes an open/close unit including two open/close members openably and closably supported about a hinge and core pieces supported by the open/close members. The core pieces are configured to open and close with the open/close members and to form an insertion space when the open/close members are closed.

Sperm, oocyte, and embryo survival and function is improved in vivo or in vitro by the use of a polysaccharide containing arabinose, galactose and/or hexuronic acid. In particular, a nonspermicidal lubricant containing such a polysaccharide (e.g., gum arabic, pectin, or galacturonic acid) increases the fertilization potential of the sperm during coitus, artificial insemination or sperm collection. Similarly, a freezing medium containing a polysaccharide containing arabinose, galactose and/or hexuronic acid enhances sperm, oocyte, or embryo viability.

The invention discloses a semi-quantitative sperm concentration rapid self-test kit, a prepration method and a using method thereof, the semi-quantitative sperm concentration rapid self-test kit comprises a sperm collection box, cell staining solution, physiological saline and a test board, wherein, the sperm collection box consists of a plastic collection box and a sperm liquefying agent, the test board comprises a housing and a substrate, a test hole and a control hole are formed in the center of the housing, water-absorbing paper, a filter membrane and a standard color unit are arranged onthe substrate, and the color of the standard color unit is the color of the test results of adding sperm samples with the concentration of 5 million, 10 million, 20 million, 30 million, 40 million, 60million, 80 million and 100 million/ml into the cell staining solution. The semi-quantitative sperm concentration rapid self-test kit has the advantages that the semi-quantitative sperm concentrationrapid self-test kit can rapidly and accurately detect the concentration of sperm and further diagnose whether a man can produce offspring or not, compared with the traditional diagnosis method, the semi-quantitative sperm concentration rapid self-test kit can save a lot of time, the use is simple, the semi-quantitative sperm concentration rapid self-test kit can be purchased at a pharmacy and used at home, thereby avoiding the feeling of shyness of a user.

The invention discloses a fish sperm preserving fluid which is prepared from 2.120-2.420g of NaCl, 1.602-2.202g of KCL, 0.178-0.478g of CaCl2, 0.133-0.333g of MgSO4.7H2O, 0.114-0.414g of NaHCO3, 0.128-0.328g of VC and 500mL of distilled water. The fish sperm preserving method comprises the following steps of: (a) sperm collection: wiping and drying the genital pore region of a mature milter by using a towel, slightly pressing the abdomen of the mature milter, and collecting the extruded sperm by using a suction tube; and (b) sperm preservation: putting the collected sperm into a clean container, adding the sperm preserving fluid of which the volume is 10-20 times of the volume of the sperm, and preserving in low-temperature light-tight places.

The invention provides a sperm collection analysis system, which comprises a sperm collection function and a sperm quality analysis function. The sperm collection device is used for stimulating a vagina environment, the sperm collection strength is controllable, and the sperm collection process is easy and pleasant with massaging, pumping, sucking, vibration and other modes for massaging stimulation, reciprocating stimulation and multimedia video stimulation. Sperm quality analysis comprises a digital microscope system and a computer sperm analysis software system, which are used for analyzing the number of sperms, sperm survival rate classification, movement speed classification and other related parameters. The sperm collection analysis system is integrated with sperm collection and sperm analysis, so that the cleanness and freshness of collected sperms and objective truth of sperm quality analysis data can be ensured. The sperm collection device can be used in prospermia and desensitization exercise.

The invention discloses a compound feed for breeding boars. The compound feed comprises the following components in percentage by weight: 60-66% of corn flour, 3-5% of fish meal, 4-7% of fermented soybean meal, 10-15% of soybean meal, 10-11% of bran, 0.3% of multiple minerals, 0.5% of table salt, 0.04% of multiple vitamins, 0.12% of choline, 1.2% of dicalcium phosphate, 0.02% of phytase, 1.2% of stone powder, 0.12% of lysine, 0.03% of a probiotics compound and 0.5-1.0% of a Chinese herbal medicine compound for producing sperm. The compound feed disclosed by the invention is reasonable in formula design, provides comprehensive balanced nutrition for breeding boars, and creatively provides the Chinese herbal medicine compound for producing sperm, so that difficult problems that the semen quality is reduced and the sexual desire of the boars is poor caused by large sperm collection intensity of the breeding boars are solved; and the Chinese herbal medicine compound for producing sperm has the effects of nourishing the liver, nourishing the kidney, benefitting sperm, replenishing blood, reinforcing the spleen, invigorating the stomach and the like, so that after the breeding boars eat the compound feed, the sexual desire of the breeding boars is strengthened, the sperm viability, the sperm density and the semen volume are obviously improved, and the sperm abnormality rate is obviously reduced.

A method of artificially inseminating a hen includes the steps of securing a male bird, applying a semen extender liquid to a tip of a cloacal protuberance of the male bird, squeezing the cloacal protuberance to extract semen from the male bird into the semen extender liquid to form a mixture, applying a tip of a pipette to the mixture, drawing the mixture into the pipette, securing the hen, inserting the pipette into the hen's cloaca, and squeezing a bulb of the pipette to inject the mixture from the pipette into the cloaca. A holding device for restraining a bird during sperm collection and artificial insemination procedures has a base, an arm extending upward from the base, and an open-ended receptacle connected to the upper end of the arm. The holding device is useful in mitigating injury to the bird and the handler during the procedures.

The invention relates to a preferential sperm freezing culture device which comprises freezing slide glass, a sperm tank, a sperm swimming channel, a collecting tank and a blocking piece. The volume of the sperm tank ranges from 50 microliters to 500 microliters, the volume of the collecting tank ranges from 5 microliters to 20 microliters, the width of the sperm swimming channel ranges from 10 micrometers to 30 micrometers, and the sperm swimming channel is connected with the sperm tank and the collecting tank. The preferential sperm freezing culture device has the advantages that the preferential sperm freezing culture device is applicable to screening preservation of frozen sperm, motile sperm can be automatically separated by the design of the sperm tank, the sperm swimming channel andthe collecting tank, operation amount is decreased, mechanical damage to the sperm caused by manual picking is avoided, the proportion of usable motile sperm is increased, the freezing slide glass ofall existing sperm can be completely replaced, the preferential sperm freezing culture device can be applied to the fields of normal sperm optimization sperm periodic IVF (in-vitro fertilization), ICSI (intracytoplasmic sperm injection) or artificial insemination, sperm collection of oligospermia and asthenospermia patients, surgical sperm extraction of azoospermatism patients, sperm collection and the like, and has a wide popularization and application prospect.

The invention discloses a sperm optimal selection device based on microtubule passages. The sperm optimal selection device comprises a sample tank and a sperm collection tank, obstruction blocks in one to three stages are arranged between the sample tank and the sperm collection tank, microtubule passages are overspreaded on the obstruction blocks in a direction from the sample tank to the sperm collection tank, the inner diameters of the microtubule passages are 10-200 micrometer, high-quality and high-activity sperm preferentially reach the sperm collection tank by using the initiative attracting of a sperm nutrient solution to sperms, the blocking of the obstruction blocks, and microporous competitive passing, and under an effect of activity competition, the sperms in the sperm collection tank are highly energetic. According to the method provided by the invention, the selection is simple, the sperm recovering ratio is high, sperm quality reduction is avoided at the same time, and the selecting effect is good. Therefore, sperm optimal selection device based on microtubule passages has the advantages of simple selection, good selecting effect, high recovering rate, and easiness in sperm quality control.

A system for purifying sperm for use in an assisted reproductive technique procedure is provided. The system includes a sperm filter through which a chemoattractant and a sperm specimen are pumped. When the chemoattractant is pumped into the filter a gradient is formed of chemoattractants having varying concentrations. Sperm that is desirable will swim toward the higher concentrated chemoattractant and are collected by a sperm collection outlet, while sperm that are immotile and unpreferred are unable to swim across the gradient are collected by a waste collection outlet.

The invention relates to the field of feeds for livestock and poultry and particularly relates to a formula feed for improving the reproductive performance of a laying breeder cock. The feed is prepared from the following raw materials in percentage by weight: 180-220 parts of maize meal, 45-50 parts of cottonseed meal, 50-60 parts of sunflower seed meal, 45-50 parts of barley straws, 20-25 parts of wheat middling, 2-3 parts of imported fish bone meal, 1-2 parts of loach powder, 1-2 parts of herba epimedii, 1-2 parts of cistanche deserticola, 1-2 parts of morinda officinalis, 1-2 parts of snail powder, 1-2 parts of fresh oyster dry powder, 2-3 parts of Chinese yams, 3-4 parts of agrimony, 1-2 parts of abelmoschus esculentus moench, 3-4 parts of lactuca indica, suitable amount of table salt and 4-5 parts of a phagostimulant. According to the feed, pure natural Chinese herbal medicines including the herba epimedii, the cistanche deserticola and the morinda officinalis are added so as to improve the quality of seminal fluid of the cock and enhancing the sexual desire of the cock; components including the snail powder, the fresh oyster dry powder and the abelmoschus esculentus moench have the very high medicinal values and can avoid a phenomenon that the seminal fluid of the cock is less, caused by a high-frequency semen collection process; the sperm collection amount of the breeder cock raised by the feed is increased and the sperm motility rate is high.

The invention discloses a sperm bank identity verification and process management system based on a three-dimensional face recognition technology, and belongs to the technical field of information management. The system comprises a three-dimensional face recognition device, a PC terminal, an identity card reader, an embedded face recognition terminal, a reception office, a physical examination room, a specimen reception office, a sperm collection room and a database. The PC terminal is in control connection with the three-dimensional face recognition device and the identity card reader, and the problems that in existing sperm bank management, identity verification of sperm supply volunteers and self-sperm storage patients is difficult, work of workers is heavy, and the work efficiency is low are effectively solved.

The invention discloses an artificial insemination method capable of improving pigeonfertilization rate. The artificial insemination method capable of improving pigeonfertilization rate comprises the following specific steps of: (1) artificially supplementing illumination 12 to 15 hours per day for continuous 20 to 40 days starting from time when domestic pigeons are 170 to 180-day-old, and collecting sperms starting from time when the pigeons are 190 to 220-day-old; (2) increasing the amounts of vitamin E and crude proteins in daily diet one week before sperm collection of male pigeons, supplying vitamin A and vitamin C according to regular amounts and maintaining metabolic energy at 19.0MJ/kg to 20.5MJ/kg; (3) performing sperm collection once every 2days at 9 to 10 a.m. in spring, autumn and winter and at 4 to 5 p.m. in summer, stopping sperm collection for 1 day per every 3 days of continuous sperm collection, diluting and uniformly mixing sperm fluid which is collected from 3 to 5 pigeons at each time and then completely inseminating the sperm fluid within 20 minutes. According to the method disclosed by the invention, by artificially supplementing illumination of 5500 to 5800K color temperature to domestic pigeons and adopting anutrient controltechniqueof adding vitamin E and crude proteins into the feed,assisted by comprehensive techniques such as sperm mixing, insemination and control of ambient temperature and insemination frequency during insemination, the breeding egg fertilization rate in pigeon production is improved.

The invention discloses a sperm collector with a wriggling structure. The sperm collector comprises a driving mechanism, a wriggling mechanism, and a sperm collection rubber sleeve driven by the driving mechanism and the wriggling mechanism. The wriggling mechanism is arranged on a support of the driving mechanism. The wriggling mechanism comprises a second motor fixed in the support of the driving mechanism, driving gears fixed to an output shaft of the second motor, crankshafts linked with the driving gears, limiting shafts, and wriggling pieces penetrating through the crankshafts and the limiting shafts. The crankshafts are driven by the second motor to rotate, so as to drive the wriggling pieces to wriggle in the radial direction. The sperm collection rubber sleeve is fixed in the wriggling mechanism. The wriggling pieces are located on the periphery of the sperm collection rubber sleeve. The sperm collection rubber sleeve is pressed through the radial wriggling of the wriggling pieces. By the adoption of the technical scheme, the sperm collection rubber sleeve is linked with the driving mechanism and the wriggling mechanism, and the sperm collection rubber sleeve can have three motion modes by controlling a first motor and the second motor in a rotating mechanism and the driving mechanism. The wriggling structure is simple in design and stable in operation.

The invention discloses a high-survival-rate sheep oosperm culture method for Hu sheep embryo in-vitro culture. The high-survival-rate sheep oosperm culture method comprises the following steps: (1) oocyte collection: sucking oocytes from ovaries of Hu sheep; (2) sperm collection: extracting sperms from the male Hu sheep; (3) fertilization: carrying out artificial fertilization on eggs and sperms;(4) embryo culture: putting the fertilized eggs into an incubator for culture; and (5) observation: observing the development condition of the embryo, wherein the incubator in the step (4) comprisesa main body, a feeding device, a rotating frame, a culture device and a discharging device, wherein the feeding device comprises a feeding channel, a conveying mechanism, a feeding opening, a rotatingtable and a transition mechanism. According to the method, the embryo survival rate is increased through artificial fertilization, the transition mechanism isolates the embryos from outside air whenthe embryos are placed into the incubator, so that embryo development is prevented from being influenced, and the discharging device realizes the purpose of independent taking a culture dish, so thatthe survival rate of embryos is increased.

The invention discloses a micromanipulation dish and a sperm selection method for preferred sperms. The micromanipulation dish for the preferred sperms comprises a dish pan and a dish cover; concave sample pool and selection passageways are arranged on an upper surface of the dish pan, wherein the selection passageways comprise a first passageway, a second passageway and third passageways; the sample pool is connected with a midpoint of the second passageway via the first passageway; a plurality of induction chambers are separately arranged on the two sides of the midpoint of the second passageway; the upper surface of the dish pan is further divided into a sperm collection area, a sperm braking area and a micro-injection area; two ends of the second passageway are respectively connected with the sperm collection area via the third passageways which slope upwards; and the sperm braking area and the micro-injection area are arranged close to the micro-injection area. Being utilized, themicromanipulation dish for the preferred sperms is capable of simulating motion trajectory of sperm in female genital tracts, as well as adsorption of apoptotic sperms by connexin V and regulation ofprogesterone on sperm functions, so that, selected preferred sperms can be immediately used for intracytoplasmic sperm microinjection, thereby beneficially improving fertilization rate of in vitro fertilization.

The invention belongs to the technical field of marine organisms and particularly relates to a method for inducing and collecting high-quality sperms of haliotis discus hannai. The method comprises the steps of selection of high-quality male parent abalones, drying stimulation of the parent abalones in shade, preparation of ozone seawater, sperm collection, sperm motility detection and determination of the fertilization rate and the hatchability. According to the method, 3-4-year plump male parent abalones with gonads mature are selected, drying stimulation in shade is performed, the parent abalones are placed in seawater under the ultraviolet irradiation and arranged in the quiet and dark environment so as to release the sperms, and the high-concentration sperms are collected by centrifugation. The collected abalone sperms are subjected to artificial insemination, and the fertilization rate is over 95%. Practice proves that the abalone sperms collected in the abalone collection mode can be applied to production time and research. The method for inducing and collecting the high-quality sperms of the haliotis discus hannai is of great significance for artificial breeding, germplasm conservation and genetic diversity protection and the like of the haliotis discus hannai.

The invention provides a group of biomolecular markers and a kit for accurate typing of azoospermia, the molecular markers are seminal plasma exosome mRNA, and the mRNA comprises PRM2, ADAM28, WFDC13, WFDC9, SEMG2 and PSA. The seminal plasma exosome is used as a marker source and is focused to genital gland specific mRNA, and the specific mRNA expression of each part in the seminal plasma exosome is comprehensively detected, so that whether obstruction exists or not and obstruction parts or sperm collection outcome is determined. The kit disclosed by the invention is based on the RNA enrichment effect and the stability of exosomes, and is combined with seminal plasma exosome long-chain RNA template amplification and qRT-PCR detection technologies to amplify and acquire a genitalia specific mRNA signal in seminal plasma, so that the sensitivity and the stability of detection are realized.

The invention provides a cryopreservation method of grouper sperms, and belongs to the technical field of sperm preservation. The cryopreservation method comprises raw materials in parts by weight asfollows: 32-52 parts of NaCl, 28-57 parts of KCl, 21-40 parts of CaCl2, 20-61 parts of trehalose, 18-44 parts of liquid nitrogen, 20-68 parts of an anti-freezing liquid and 26-50 parts of compound antibiotics. By combination with the cryopreservation method comprising steps of sperm collection, dilution, precooling and quick-freezing for low-temperature storage of a seminal fluid, the reactivatedsperms can keep vigor of fresh sperms by the aid of a reasonable cooling rate, and the cryopreservation method has great significance for establishment of a marine fish sperm bank and protection for germplasm resources and biodiversity.

The invention discloses a sperm cryopreservation method of scatophagus argus. The sperm cryopreservation method comprises four steps of sperm collection, sperm dilution, sperm cryopreservation and sperm thawing, firstly male scatophagus argus is selected to inject human chorionic gonadotropin into dorsal muscle, then the sperm of the scatophagus argus is collected by an artificial abdominal extrusion method, then the sperm and antifreezing liquid are mixed to obtain preservation liquid of the scatophagus argus sperm, a diluent is prepared by mixing any one of a TS-2 diluent, a TS-19 diluent, aHank's solution, a Cortland diluent and sterilized seawater with dimethylsulfoxide; the sperm preservation liquid is injected into a cryopreservation tube, then freezing is conducted by adopting a step-by-step cooling method, and then the cryopreservation tube is immersed in liquid nitrogen for long-term preservation; when taking for use, the cryopreservation tube containing the sperm preservation liquid is taken out from the liquid nitrogen, and then is placed in a water bath with the temperature of 42 DEG C. According to the sperm cryopreservation method, the sperm of the scatophagus arguscan be preserved for a long time, and the sperm survival rate and insemination rate after thawing and resuscitation are high.