138 results about "Sperm Collection" patented technology

The invention discloses Pseudomonas sp. W50 and an application of the Pseudomonas sp. W50 in degradation of xanthate by combining the Pseudomonas sp. W50 with Cynanchum otophyllum. The Pseudomonas sp. W50 is preserved in Guangdong Microbial Culture Collection Center on July 31, 2024, and the preservation number of the Pseudomonas sp. W50 is GDMCC NO.64924. The Pseudomonas sp. W50 is preserved in the Guangdong Microbial Culture Collection Center on July 31, 2024. The degradation rate of 100mg / L BuX in 9h reaches up to 99%, and the strain also has various growth promoting characteristics of dissolving phosphorus, dissolving potassium, secreting IAA and GA and degrading cellulose. The BuX-polluted wastewater remediation agent can be used for remediation of BuX-polluted wastewater, can play a synergistic interaction role when being combined with pinkflower clover herb, has a more remarkable remediation effect, provides a new method for treatment of BuX pollution and promotion of healthy growth of aquatic plants in mining areas, and has a wide application prospect.

The invention discloses an HNRNPA2B1 gene-based bovine sperm malformation rate related SNP molecular marker and application thereof, and belongs to the technical field of genetic molecular biology, the number of the SNP molecular markers is two, SNP1 is at the position of a bovine genome chr4: 69733358, the polymorphism is C or A, SNP2 is at the position of a bovine genome chr4: 69733387, and the polymorphism is T or G. According to the two SNP loci provided by the invention, the dominant genotypes of g.-1713A > C and g.-1684G > T are AA and GG respectively, and the sperm collection quantity and the sperm malformation rate of the two SNP loci have better performance. By detecting the polymorphism of the two SNP loci, the motility evaluation of the bull sperms can be realized, the breeding is assisted, a reliable support is provided for breeding a high-quality next generation, and the method has the advantages of high reliability, high efficiency, low cost and the like.

The invention provides bacillus and application thereof in separation and recovery of heavy rare earth, the strain of the bacillus is named as Bacillus sp.DW011 and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 65275. The invention belongs to the technical field of biological environmental protection, provides novel bacillus, enriches the prior art, realizes application of the novel bacillus in separation and recovery of heavy rare earth, has extremely strong stress resistance and good adaptability to the external environment, and is beneficial to selective adsorption and recovery of heavy rare earth ions.

The invention discloses bacillus (Bacillus sp.) GB-1. The preservation number of the bacillus (Bacillus sp.) GB-1 in Guangdong Microbial Culture Collection Center is GDMCC NO: 65601. The bacillus (Bacillus sp. The strain GB-1 has a good effect of degrading quorum sensing signal molecules OHHL, the quorum quenching effect of the strain is stable and remarkable, a new way and method are provided for biological control of AHLs-mediated pathogenic bacteria, the strain has huge application potential, the use of chemical pesticides can be reduced, and the cost is reduced. A new way is provided for replacing chemical prevention and control with biological prevention and control, and the application prospect is good.

The invention relates to a ganoderma lucidum strain L4905 and a cultivation method and application thereof, and belongs to the technical field of biology. The ganoderma lucidum strain L4905 is preserved in Guangdong Microbial Culture Collection Center on October 9, 2023, and the preservation number is GDMCC No: 63866. The ganoderma lucidum strain L4905 is short in growth cycle and stable in character. The strain has the characteristics of high gloss of sporocarp paint sample, kidney-shaped pileus, thick mushroom pulp, high yield of sporocarp and spore powder, stable cultivation character, high polysaccharide content and the like, and is suitable for substitute cultivation and popularization in most areas of Yunnan.

The present invention relates to the field of microorganisms, and in particular to a strain of Lactobacillus brevis ZG-7 and its application. The Lactobacillus brevis ZG-7 has been preserved in the Guangdong Provincial Microbial Culture Collection Center on June 14, 2023, with a preservation number of GDMCC No.63562. The Lactobacillus brevis ZG-7 of the present invention has a strong inhibitory ability against Salmonella typhimurium, and has good acid and alkali resistance and bile salt resistance, and the inhibitory property has good thermal stability and ultraviolet stability. The Lactobacillus brevis ZG-7 of the present invention can be used to regulate the microecological balance in the intestine of animals, enhance the nonspecific immune function of the body to prevent diseases, and can also provide nutritional factors, promote the digestion and absorption of nutrients, promote animal growth and improve feed conversion rate.

The invention discloses trichoderma asperellum TR35 and application thereof in inhibition of mulberry bacterial wilt and prevention and treatment of mulberry bacterial wilt, the trichoderma asperellum TR35 is identified as Trichoderma asperellum, the trichoderma asperellum TR35 is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC No.64886, and the preservation time is July 18, 2024. The trichoderma asperellum TR35 obtained through separation and screening has good inhibition and antagonism effects on mulberry ralstonia solanacearum, and can be used for preventing and treating mulberry bacterial wilt. The inhibition rate of the trichoderma asperellum TR35 to the mulberry ralstonia solanacearum reaches up to 98.25%, and the active fermentation liquor of the trichoderma asperellum TR35 not only can significantly inhibit the growth of the ralstonia solanacearum and reduce the forming ability of a biological membrane, but also can destroy the integrity and continuity of the biological membrane of the ralstonia solanacearum. In addition, the bacterial zone of the ralstonia solanacearum can be reduced, the athletic ability is reduced, and the expression of virulence-related genes in the ralstonia solanacearum is remarkably inhibited.

The invention provides a Klebsiella strain LBTRJ-2BN and application thereof, and belongs to the technical field of microorganisms. The Klebsiella sp. LBTRJ-2BN is preserved in the Guangdong Microbial Culture Collection Center, the address is the 5th floor of the building 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City, the preservation date is December 25, 2024, and the preservation number is GDMCC No: 65677. The Klebsiella sp. LBTRJ-2BN provided by the invention can grow under the selective pressure of high-concentration catechol, and in the presence of other carbon sources, the catechol natural degrading bacteria still have better degrading capability on catechol. The Klebsiella sp. LBTRJ-2BN provided by the invention enriches catechol microbial degradation bacterium resources, provides strain resources and method reference for biological treatment of organic pollutants such as catechol, and has feasible application value.

The present invention belongs to the field of new microbial application technology, specifically a Ludwig's Enterobacter and its application in improving inflammatory bowel disease. The strain is named Ludwig's Enterobacter (Enterobacter ludwigii) LS-57, which is deposited in the China General Microbiological Culture Collection Management Center on July 22, 2024, and the deposit number is CGMCC NO. 31424. The strain has the ability of cluster movement, has a strong adaptability to the intestinal environment, tolerates the acidity of gastric juice well, is sensitive to a variety of commonly used antibiotics, and has a good improvement effect on colitis.

The invention discloses lactobacillus plantarum WL05 capable of efficiently degrading ethanol and application of the lactobacillus plantarum WL05, and belongs to the technical field of biology. The bacterial strain is lactobacillus plantarum WL05, the bacterial strain is preserved in the China General Microbiological Culture Collection Center on July 8, 2024, and the preservation number is CGMCC No.31202. The lactobacillus plantarum WL05 has relatively high acid production capacity and activity, and the lactobacillus plantarum WL05 is preserved in the China General Microbiological Culture Collection Center on July 8, 2024. The acid resistance and cholate resistance are high; after the lactobacillus plantarum WL05 is fermented in an MRS culture medium containing 10% ethanol, the ethanol degradation rate can reach 42.5%, and the ethanol dehydrogenase activation rate is 43.53%; the fermentation liquor is inoculated into a lalang grass rhizome-reed rhizome matrix culture medium, and after fermentation, the measured ethanol degradation rate is 63.9%, and the activation rate of ethanol dehydrogenase in the fermentation liquor is 66.5%. The strain has strong reducing ability and antibacterial ability at the same time. The ethanol degradation rate of the beverage prepared based on the strain can reach 32%. The product can be used for preparing functional food for dispelling effects of alcohol and protecting liver, and has high application value in the field of functional food.

The invention provides a fusarium oxysporum FOZJ2024 strain and an application of the fusarium oxysporum FOZJ2024 strain. The strain is preserved in Guangdong Province Microbial Culture Collection Center on November 20, 2024, the preservation number is GDMCC No: 65511, the preservation address is the fifth floor of building 59, No.100 Courtyard, Xianlie Middle Road, Guangzhou City, and the strain has an excellent killing effect on crape myrtle leaf aphid, mealybug, Spodoptera frugiperda and Solenopsis invicta, and is suitable for preventing and treating plant insect pests and agriculture and forestry harms caused by the crape myrtle leaf aphid, mealybug, Spodoptera frugiperda and Solenopsis invicta.

The invention discloses double-fermentation clostridium parafusiforme, application thereof and a molecular marker for detecting the double-fermentation clostridium parafusiforme, and belongs to the technical field of microorganisms and biomedicine. The invention discloses a double fermentation clostridium parafermentum S1-GT1, which is preserved in the Guangdong Microbial Culture Collection Center on May 27, 2024, and the preservation number of the double fermentation clostridium parafermentum S1-GT1 is GDMCC (China General Microbiological Culture Collection Center) NO: 64683. The invention also discloses an application of the S1-GT1 strain in preparation of drugs for treating solid tumors, and a molecular marker for identifying the S1-GT1 strain. The double-fermentation clostridium parafusiformis S1-GT1 disclosed by the invention has good safety and excellent spore production capacity, and can be used for inhibiting the growth of one or more solid tumors and reversing or destroying one or more solid tumors.

The present invention discloses a Bacillus velezensis GY1, which was deposited in the Guangdong Provincial Microbial Culture Collection Center on June 28, 2024, with a deposit number of GDMCC No: 64792. The Bacillus velezensis GY1 provided by the present invention can degrade 100% and 56.4% of nitrogen in M9 culture medium containing 200 mg / L and 1000 mg / L ammonia nitrogen, respectively, and the ammonia reduction rate in the actual production of aquaculture water reaches 53.3%. It also has a strong inhibitory effect on common pathogens in aquatic products: Vibrio alginolyticus, Salmonella and Vibrio parahaemolyticus.

The invention discloses lactobacillus arrhizatae and application thereof, and relates to the technical field of microorganisms. The lactobacillus arrhizatae is preserved in the Guangdong Microbial Culture Collection Center on December 10, 2024, the preservation number is GDMCC 65603, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. According to the invention, a strain of Lactobacillus argenteus is screened from healthy free-ranging adult local partridge chickens from Guangxi Zhuang Autonomous Region Tibetan City, the strain is named as LB19, and verification proves that the Lactobacillus argenteus has a good inhibition effect on food-borne pathogenic bacteria including salmonella and escherichia coli, and can be used for preparing food-borne pathogenic bacteria. Good application potential is realized in the aspect of preventing and treating food-borne pathogenic bacteria.

The present invention provides a Streptomyces, named: Streptomyces coffeae , preserved in Guangdong Province Microbial Culture Collection Center, with the preservation number of GDMCC No: 64532. The Streptomyces and its fermentation liquid of the present invention have good antibacterial properties against banana wilt No. 4 physiological subspecies, gloeosporium anthracnose, cucumber wilt, banana anthracnose, litchi anthracnose, mango anthracnose, strawberry anthracnose, banana leaf spot pathogen, rice blast pathogen, banana gray spot pathogen, mango leaf blight pathogen, etc., and the antibacterial rate reaches more than 50%. Moreover, the growth of banana seedlings can be effectively promoted under the attack of pathogens, and the product has resistance to multiple antibiotics. The product is a potential biological agent for preventing and treating anthracnose diseases, wilt diseases, etc. and promoting plant growth, etc., and has broad development space and good development and application prospects.

The invention provides a method for producing lysergic acid by fermentation, wherein the seed liquid of Claviceps paspali is inoculated in a fermentation medium for liquid fermentation culture, wherein the Claviceps paspali is deposited in the China General Microbiological Culture Collection Center, and the deposit number is CGMCC NO.41366, and the fermentation medium, based on its total volume, comprises 80-150 g / L of carbon source and 40-90 g / L of nitrogen source, wherein the carbon source comprises corn starch hydrolyzed by alpha-amylase, and the content thereof is 50-100 g / L. The content of lysergic acid in the fermentation liquid obtained by the inventive method can reach 4.5 g / L, and the fermentation medium uses corn starch as the main carbon source, and the cost is low.

The present invention belongs to the field of microbial technology, and specifically discloses a preparation method and application of a Pseudochrobactrum asaccharolyticum and a preparation thereof, wherein the Pseudochrobactrum asaccharolyticum is deposited in the Guangdong Microbial Culture Collection Center with a deposit number of GDMCC NO: 62528 and a deposit date of July 7, 2022, and is named Pseudochrobactrum asaccharolyticum Z11. The Pseudochrobactrum asaccharolyticum Z11 screened by the present invention has the ability to produce alkali and has a large tolerance to Cd(II), provides a new strain resource for cadmium passivation alkali-producing microorganisms, and can be used to repair cadmium-contaminated soil.

The invention belongs to the technical field of artificial insemination of bumblebees, and particularly relates to a male bumblebee sperm collecting device. The male bee seminal vesicle extraction platform is arranged on the base, the male bee seminal vesicle extraction platform is used for clamping the male bee seminal vesicle glass sheet, the male bee seminal vesicle glass sheet is used for placing a male bee seminal vesicle, and the male bee seminal vesicle extraction platform drives the male bee seminal vesicle glass sheet to move in the horizontal direction of the base; the insemination needle fixator is vertically arranged on the base in a lifting mode and used for fixing a glass needle, and the glass needle faces the male bee seminal vesicle glass sheet. Through the device, the extraction efficiency can be remarkably improved, so that the sperm exposure time is effectively shortened, and relatively high vitality is kept.

The present invention provides a probiotic combination for inhibiting Helicobacter pylori, Lactobacillus rhamnosus INM3105, which was deposited in Guangdong Microbial Culture Collection Center on April 11, 2024, with a deposit number of GDMCC NO: 64513. Lactobacillus plantarum INM3106 was deposited in Guangdong Microbial Culture Collection Center on April 11, 2024, with a deposit number of GDMCC NO: 64514. The advantages are: the peptidoglycan extracted from Lactobacillus rhamnosus and Lactobacillus plantarum can effectively inhibit Helicobacter pylori. The ability of the peptidoglycan ratio of the two strains to inhibit Helicobacter pylori is further improved than that of a single strain of the same concentration.

The application provides a method for on-site rapid artificial insemination of wild Pseudosciaena crocea of Lutjanidae, and belongs to the technical field of aquatic animal breeding. Wild parent fish of Pseudosciaena crocea of Lutjanidae are captured in the breeding season; sperm is collected from the selected sexually mature male parent fish on the capture site; after artificial sperm collection, sexually mature female parent fish are selected, and fish eggs are collected; high-quality fertilized eggs are obtained through artificial insemination; the artificial insemination comprises the following steps: adding sperm into fish eggs, adding natural seawater for stirring, and finally washing with natural seawater. Through comprehensive regulation of key links in the whole process of artificial insemination, the purpose of obtaining high-quality fertilized eggs by using wild parent fish of Pseudosciaena crocea of Lutjanidae and carrying out artificial fry breeding is achieved.

The semen collection cup comprises a cup body and a cover body, the cover body comprises a cup cover and a silica gel layer, the bottom wall of the cup cover is covered with the silica gel layer, the silica gel layer can be embedded into the cup body and abut against the inner wall of the cup body, and the outer wall of the cup body is covered with a heat preservation coating. The structure can improve the sealing performance and prevent the seminal fluid sample from leaking in the transportation process; the cup body can be conveniently closed and opened, one-hand operation is facilitated, the heat preservation effect can be improved, and the influence of temperature change on the activity and quality of a sample is reduced.

The utility model relates to the field of artificial insemination, in particular to a semen collection device, which comprises a semen collection device body which comprises a semen collection device, and is characterized in that the body further comprises a holding part connected with the semen collection device, the holding part comprises a telescopic component with two ends extending out of the body, and the telescopic component is provided with an opening. The grips are arranged at the two ends of the telescopic component; according to the semen collection device, the holding part is additionally arranged on an original semen collection device, the holding part is formed by combining the telescopic component and the holding handle, in the actual operation process, the holding handle provides the corresponding holding effect, instability in the collection process is reduced, and the operation difficulty is lowered; the telescopic component is convenient to store, due to the fact that the grip is used, the transverse occupied space of the whole semen collection device can be increased, under the action of the telescopic component, the grip retracts into the body, and therefore the problem that storage is inconvenient is solved.

The present invention discloses Lactobacillus reuteri A-1 and its application in preparing iron supplement and intestinal regulation products, belonging to the field of microorganisms and biomedicine. The present invention provides a Lactobacillus reuteri ( Limosilactobacillus reuteri )A‑1, deposited in Guangdong Microbial Culture Collection Center, the deposit date is July 8, 2024, and the deposit number is GDMCCNO: 64837. Based on this Lactobacillus reuteri, a postbiotic product has been successfully developed. It has been verified that it has the effect of regulating the balance of intestinal flora, promoting human digestion and absorption, and effectively supplementing iron. Daily administration of 100 mg of a postbiotic product containing 16 billion strains and metabolites of the present invention can sustainably provide endogenously required amounts of glutathione, lycopene, and arginine during intestinal reproduction and growth, can effectively supplement iron, and has good practical application value.

The present invention relates to the field of agricultural microbial technology, and specifically discloses a method for recruiting root endophytic fungi by a rhizobium GZHC2-2 strain and its application in alleviating sensitive soybean waterlogging stress. The rhizobium (Rhizobium multihospitium) GZHC2-2 strain was deposited in the Guangdong Provincial Microbial Culture Collection Center on January 4, 2023, with a deposit number of GDMCC No.63032. This strain has the ability to help soybeans resist waterlogging. Inoculation of the rhizobium under waterlogging stress can increase the aboveground and underground biomass, the number of nodules and the fresh weight of nodules of soybeans. At the same time, the ability of waterlogging-sensitive soybean varieties to enrich beneficial root endophytic fungi (such as Sordariomyceta, Moesziomyces, Rhynchogastremataceae, Eurotiomycetes, Ustilaginaceae) is induced. After purifying and isolating the root endophytic fungi, the enriched beneficial fungi can also significantly increase the aboveground and underground biomass, the number of nodules and the fresh weight of nodules of soybeans under waterlogging stress. The invention can be used to improve the growth and increase the yield of soybeans under waterlogging stress, and has broad application prospects.

The utility model discloses a pepper stamen fine powder taking device which comprises a mounting shell, a screening barrel, a blanking barrel, a driving mechanism and a stirring mechanism, the mounting shell, the screening barrel and the blanking barrel are detachably connected together in sequence from top to bottom, and a filter screen is arranged at the bottom of the screening barrel; the stirring mechanism comprises a rotating shaft and a stirring plate; one side of the top of the rotating shaft is rotationally connected with the mounting shell, one side of the bottom of the rotating shaft extends into the screening barrel, and the stirring plate is fixedly connected to the bottom of the rotating shaft; the driving mechanism is arranged in the mounting shell and used for driving the rotating shaft to rotate. The driving mechanism drives the rotating shaft and the stirring plate to rotate, pepper stamens in the screening barrel are stirred, separation of fine powder from the stamens can be accelerated, the separation effect is effectively improved, and the separation efficiency is improved.

The invention relates to the technical field of sperm collection, in particular to a sperm collection cup and a using method thereof.The sperm collection cup comprises a sperm collection cup body, the sperm collection cup body further comprises a cup body made of plastic and a cover on the cup body, a circle of buckling groove is formed in the lower portion of the cover, and the buckling groove is of a structure matched with the upper edge of the cup body; a circle of snap ring groove is further arranged below the cover, the sperm taking cup body further comprises a sperm containing bag made of latex and a snap ring, the snap ring is of a structure matched with the snap ring groove, the upper portion of the sperm containing bag is arranged in the snap ring groove, the snap ring is installed in the snap ring groove to fix the upper portion of the sperm containing bag, and a middle hole is formed in the cover. The semen extractor has the advantages of convenience in semen removal, convenience in use and better semen extraction effect. The invention further provides a using method of the sperm collecting cup.

The present invention belongs to the field of microorganisms, and specifically relates to a plant lactobacillus and an application thereof. The plant lactobacillus is deposited in the Guangdong Microbial Culture Collection Center, and the deposit number is GDMCC No: 63911. The plant lactobacillus is used to ferment cattle bile, and a large amount of bile salt hydrolase is produced during the fermentation process, which can convert the bound bile acid in the cattle bile into free bile acid, and a high conversion rate can be achieved within 2-3 days, and the utilization value is high.

The present invention discloses a kind of Serratia marcescens and its acid-reducing application. The strain name is Serratia marcescens (Serratia sp.) JS‑043, and the deposit number is GDMCC NO: 62046. The strain was deposited in the Guangdong Provincial Microbiological Culture Collection Center on the 5th floor of Building 59, No. 100, Xianlie Middle Road, Guangzhou on November 9, 2021. The Serratia sp.JS‑043 strain in the present invention has excellent tolerance to high concentrations of citric acid, and can improve its pH value by biotransforming plant acidic substances such as organic acid compounds in citrus juice and citrus residues, reduce or eliminate the inhibitory effect of low pH conditions on the activity of fermentation microorganisms, and effectively improve the utilization efficiency of acidic organic matter such as citrus juice.

A breeding rabbit sperm in-vitro obtaining and culturing device comprises a bottom box, a box cover is arranged at the top of the bottom box, the two ends of the box cover are of arc-surface-shaped structures, a collecting device used for collecting sperms is detachably installed at the end of the bottom box, a plurality of containing grooves used for containing the collecting device are formed in the side face of the bottom box, and groove doors capable of being opened and closed are arranged at the outer ends of the containing grooves. Symmetrical first inserting columns are fixed to the inner side of the groove door, first inserting holes matched with the first inserting columns are formed in the bottom box, and a handle is fixed to the outer side of the groove door. A supporting column is fixed to the bottom of the box cover, a sliding groove is formed in the top of the bottom box, the supporting column is slidably connected into the sliding groove, a vertical first strip-shaped hole is formed in the side face of the bottom box, a threaded rod is detachably fixed to the side face of the supporting column, and the outer end of the threaded rod extends out of the first strip-shaped hole and is in threaded connection with a locking nut. The sperm collecting device is convenient to take and place, the storage quality of sperms is guaranteed, the height is convenient to adjust, and the adaptability is good.

The present invention provides a strain of Bacillus Velez G14 and its application. The strain was deposited in the Guangdong Provincial Microbial Culture Collection Center on July 16, 2024, with a deposit number of GDMCC NO: 64871, and the deposit address is 5th Floor, Building 59, No. 100, Xianlie Middle Road, Guangzhou. The Bacillus Velez G14 strain and its fermentation broth of the present invention can not only effectively inhibit Pasteurella multocida, Enterobacter aerogenes, Enterococcus faecalis and Pseudomonas aeruginosa, but also have better genetic stability, acid-base stability, temperature stability, enzyme stability, ultraviolet stability and storage stability.