7 results about "Mealybug" patented technology

The invention provides a long-term maintaining method for a cotton field diaphous babaskii population based on functional plant combination and space optimization and application of the long-term maintaining method, and belongs to the technical field of biological prevention and control of agricultural pests. According to the method, functional plants are planted in the cotton field in a mode of combining strip planting and functional patch planting; leguminous plants are planted on the periphery of the cotton field in a strip mode to form a plant buffer strip; functional plaques are arranged in a cotton field in a dispersed mode, and compositae plants, umbelliferae plants and cruciferous plants are planted in the functional plaques in a mixed mode. On the basis of functional plant combination and space optimization, after one-time release of the leptosphaeria babaskii, the population is continuously and stably maintained in the cotton field for many years, the field overwintering survival rate is larger than or equal to 71.3%, the parasitism rate to the phenacoccus solenopsis is stabilized to be 80% or above, the natural enemy leptosphaeria babaskii can be effectively used for preventing and controlling the phenacoccus solenopsis in the cotton field, and the pesticide use amount is reduced.

This invention belongs to the field of biopesticide technology, specifically to a biopesticide for controlling the new pineapple mealybug. The application of *Bacillus tuvani* in the preparation of a biopesticide for controlling the new pineapple mealybug is described. The *Bacillus tuvani* was purchased from the Guangdong Provincial Microbial Culture Collection Center, number GDMCC NO.800089. The *Bacillus tuvani* bacterial solution of this invention exhibits insecticidal activity against the new pineapple mealybug, demonstrating the potential for preparing a biopesticide for its control. Furthermore, when the *Bacillus tuvani* bacterial solution is used in combination with the existing biopesticide spinosad, it has a synergistic effect in killing the new pineapple mealybug, further improving the control effect and providing support for the preparation of a biopesticide for controlling the new pineapple mealybug.

The invention relates to the technical field of microbial pesticides, in particular to a bacillus thuringiensis Bt-39 strain, a culture method of the bacillus thuringiensis Bt-39 strain, an insecticidal oil agent prepared from the bacillus thuringiensis Bt-39 strain, and application of the oil agent in prevention and treatment of Myzus persicae and Hylopterus pruni. The bacillus thuringiensis Bt-39 strain provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is May 25, 2020, the preservation number is CGMCC No.19877, and the Latin name is Bacillus thuringiensis. The 5-day fatality rate of green peach aphids and green peach aphids exceeds 68% and is superior to that of chemical agents, meanwhile, chemical pesticide residues can be reduced, and natural enemy insects are protected.

The present invention discloses a rapid detection primer set, a kit and an identification method for Jackbell's mealybugs. It relates to the field of molecular biology technology. It includes: JKB-RPA-F, JKB-RPA-R and provides related kits and identification methods. The present invention establishes a rapid detection system of rapid crude DNA extraction, RPA (recombinase polymerase isothermal amplification technology) combined with CRISPR-Cas12a. The system can complete the detection of target insects within 1.2 hours under a constant temperature of 37°C. It has the characteristics of short time, high sensitivity and strong specificity, and provides an efficient method for rapid on-site detection of Jackbell's mealybugs. Compared with traditional PCR technology and other rapid detection methods, it is more suitable for on-site detection, and suitable for promotion and application.

The invention discloses a papaya Pleurotus geesteranus trehalose synthase gene PmTPS as well as an encoding protein, dsRNA and application thereof, belongs to the technical field of molecular biology, and particularly relates to the papaya Pleurotus geesteranus trehalose synthase gene PmTPS which is PmTPS1 and PmTPS2, the nucleotide sequence of the PmTPS1 is as shown in SEQ ID NO.1, and the nucleotide sequence of the PmTPS2 is as shown in SEQ ID NO.2. The invention further discloses a preparation method of the papaya Pleurotus geesteranus trehalose synthase gene PmTPS. According to the dsRNA of the gene PmTPS, the dsRNA is PmTPS 1-dsRNA and PmTPS 2-dsRNA, the nucleotide sequence of the PmTPS 1-dsRNA is as shown in SEQ ID NO.3, and the nucleotide sequence of the PmTPS 2-dsRNA is as shown in SEQ ID NO.4. The dsRNA of the gene PmTPS comprises the dsRNA of the gene PmTPS, the dsRNA of the gene PmTPS and the dsRNA of the gene PmTPS. The dsRNA is used for inhibiting the expression of the papaya Pleurotus geesteranus trehalose synthase gene PmTPS, so that the papaya Pleurotus geesteranus is prevented and treated.

The application belongs to the technical field of biotechnology, and particularly discloses a dsFAR2@ZIF-8 nano-complex, a preparation method thereof and application of the dsFAR2@ZIF-8 nano-complex in control of cassava mealybug. The dsFAR2@ZIF-8 nano-complex is prepared by combining RNAi technology and nano materials and by wrapping dsRNA with ZIF-8 nanoparticles, and can effectively inhibit expression of a key gene FAR2 for wax synthesis of the cassava mealybug, significantly improve mortality of the cassava mealybug and significantly reduce the reproductive capacity of the cassava mealybug. Moreover, the FAR2 gene only plays a role when the cassava mealybug feeds on plants, and has obvious species specificity. Therefore, the dsFAR2@ZIF-8 nano-complex for the key gene for wax synthesis of the cassava mealybug has good advantages such as species specificity, safety and high efficiency in control of the cassava mealybug, has a good market application prospect and potential economic and ecological benefits in green control of the cassava mealybug, and can provide a new target technology and a new idea for innovative control methods of mealybugs.

The utility model discloses a stacked type large-scale mealybug breeding device, which belongs to the technical field of biological control and comprises a fixing frame and an insect breeding net, the fixing frame is of a cuboid structure and is divided into a plurality of breeding layers, a hard board is arranged at the lower end of each breeding layer, and the insect breeding net is detachably arranged in the breeding layers. The insect breeding net is of a closed cuboid structure matched with the breeding layer in specification, a soft cushion is arranged in the insect breeding net, semi-arc-shaped zipper doors are arranged on the front side and the rear side of the insect breeding net, and the two semi-arc-shaped zipper doors are arranged on one side of the insect breeding net. The mealybug breeding device provides a place suitable for growth of mealybugs, is favorable for breeding a large number of mealybugs, and can prevent the mealybugs from spreading, crawling and flying all around.