139 results about "Salmonella diarizonae" patented technology

The invention belongs to the technical field of biology and particularly relates to endolysin sourced from salmonella bacteriophage and an application thereof. The amino acid sequence of the endolysin sourced from the salmonella bacteriophage includes the total of the partial of the amino acid sequence represented as the SEQ ID No.1. Sequence structure analysis proves that the protease is a lyase which can lysing bacterial cell walls in the bacteriophage. A test proves that the protein formed by the amino acid sequence is good in bactericidal activity. The endolysin, when being expressed and purified by a suitable expression vector on market, can achieve a significant sterilization effect, so that the endolysin can be used for preparing a bacterial inhibitor of salmonella.

The invention discloses a Riemerella anatipestifer OmpA/MotB truncated recombinant protein, its antibody and a preparation method and an application thereof. The recombinant protein has immunoreactivity similar to natural OmpA/MotB protein, can bind to RA antibody specifically, will not generate cross reactions with Salmonella anatum positive serum, duck colibacilosis positive serum, duck swollen head septicemia virus positive serum, avian influenza virus (H5) positive serum, duck plague virus positive serum, duck hepatitis virus positive serum and the like, and can be used as a detection antigen for detecting whole bacteria antibody. As the recombinant protein is not whole bacteria, there is no risk of spreading bacteria when the recombinant protein is applied to detection. In addition, the preparation method of the recombinant protein is simple, is suitable for large-scale industrial production, can realize standardization of products and is beneficial to result comparison between different laboratories.

The invention discloses a preparation method and possible application of flagellin FliC from salmonella abortus equi to salmonella abortus equi immunity. Through cloning and connecting a flagellin FliC gene from the salmonella abortus equi to a prokaryotic expression vector pGEX-4T-2, a prokaryotic recombinant of a protein is obtained; the prokaryotic recombinant is transformed into engineering bacteria BL-21, and the protein is subjected to induced expression and purification; the protein has an amino acid sequence in a sequence table 1; the purified FliC recombinant protein has good immunogenicity after being used for the immunization of experimental animals, can overcome the defects of low antibody level and poor safety generated by inactivated vaccines and can be used for preparing subunit vaccines for preventing and treating equine paratyphoid infection and can be used as equine animal vaccine adjuvants, thereby having good development and application prospect.

The invention provides an antibacterial peptide and application thereof, and belongs to the technical field of biology. The antibacterial peptide is designed and obtained through a rational moleculardesign method on the basis of family analogues Pexiganan of the antibacterial peptide magainin derived from the epithelium of xenopus laevis. The antibacterial peptide is an antibacterial peptide Pexi-1 with an amino acid sequence as shown in SEQ ID NO. 1 or an antibacterial peptide Pexi-2 with an amino acid sequence as shown in SEQ ID NO. 2. The antibacterial peptide provided by the invention hasgood structural characteristics, can significantly improve the antibacterial effect on staphylococcus aureus, salmonella and aspergillus flavus, effectively reduces the hemolysis rate and cytotoxicity of chicken erythrocytes, and can be effectively used in animal-related production activities.

The invention discloses rare earth metal complexes using orbifloxacin as a ligand, a method for synthesizing the same and application thereof. In the tests of the inhibiting effect of the complexes on bacteria such as salmonella pullorum, swine streptococcosis and bovine streptococcus uberis, the results show that the complexes have quite high bacteriostatic activity, wherein all of Er(III), Sm(III) and Eu(III) complexes of the orbifloxacin have certain bacteriostatic activity on the swine streptococcosis which cannot be inhibited by orbifloxacin and doxycycline. Mouse acute toxicity tests show that the complexes have low in-vivo toxicity to mice and are highly tolerable to mice. The toxicity of various complexes meets the acute oral toxicity ungraded standards of European Community and the PRC food security assessment grade-two (actually non-toxic) standard, wherein the neodymium complex of the orbifloxacin has the lowest toxicity.

The invention discloses a chicken white diarrhea dyed condensation antigen, a preparation method and an application thereof and belongs to the technical field of veterinarian diagnosis. The antigen is prepared by following steps: performing recovery passage to chicken white diarrhea salmonella strains comprising SP7220, SP7701, SP8441 and SP9905 to obtain a seed bacterial liquid; inoculating the seed bacterial liquid on a solid culture medium for proliferation; inactivating the bacteria in formalin; regulating the concentration of the bacterial liquid through turbidimetry; and finally adding a crystal violet solution to dye the bacterial liquid, fully mixing uniformly the bacterial liquid and packaging the bacterial liquid to obtain the antigen. The preparation method is simple and reasonable, is scientific, is stable in production and is low in cost. The chicken white diarrhea condensation antigen production strain is good in antigenicity and is low in mutation rate. A prepared chicken white diarrhea dyed condensation antigen product is high in sensitivity, is strong in specificity, is quick in diagnosis and is clear in condensation image, and is an excellent dyed condensation antigen for detecting chicken white diarrhea.

The invention belongs to the technical field of medicine production research development, and relates to isolation and identification as well as application of volatile oil elements of Parasenecio firmus leaf. The isolation and identification of the volatile oil elements of the Parasenecio firmus leaf are realized by the following steps of: weighing dried Parasenecio firmus leaf, and extracting ultrasonically by a water steam distillation method to obtain the volatile oil; and analyzing the obtain volatile oil by utilizing a 6890/5973N gas chromatograph-mass spectrometer. The obtain volatile oil mainly comprises the following chemical elements in proportion: 18.98% of n-palmitic acid, 9.89% of 1-tridecylene, 8.05% of caryophyllene epoxide, 6.04% of (S)-undeca-cycloolefin, 3.84% of 9,12,15-octadecatrienoic acid, 2.97% of (-)-spathulenol, 2.83% of kaurene and 2.12% of caryophyllene. The volatile oil is used for preparing pharmaceutical preparation capable of restraining escherichia coli, streptococcus, Salmonella, Pasteurella and staphylococcus aureus.

The invention relates to a test paper box which is based on a bacterial fluorescent staining technique and is used for detecting dyed salmonella typhimurium via immunochromatography by utilizing a single antibody. The test paper box comprises a single antibody test strip and a fluorochrome box. The test paper box has the advantages that the salmonella typhimurium is dyed in advance by utilizing fluorochrome and then is sprayed on a T line of the test strip by utilizing a specific antibody of the salmonella typhimurium so as to capture the salmonella typhimurium in a mixed sample, so that the salmonella typhimurium in the sample can be rapidly and specifically detected.

The invention discloses a salmonella source tracing typing method based on a gMLST (genome multilocus sequence typing) technology. The method comprises the steps as follows: (1) isolation and dentification of salmonella; (2) DNA extraction and content detection; (3) whole-genome sequencing and gMLST typing; (4) MLST typing; (5) drug resistance gene analysis and virulence gene analysis; and (6) cladogram analysis. The method establishes a salmonella gMLST technology for important food-borne pathogens such as salmonella, staphylococcus aureus or vibrio parahaemolyticus to be applied to epidemiological source tracing, so that the method is promoted to source tracing of food-borne pathogens.

The invention discloses a preparation for producing a COVID-19 antibody after oral administration and a preparation method of the preparation. Attenuated salmonella typhimurium is used as a carrier todeliver DNA vaccine, the preparation effectively reaches intestinal tract and delivers DNA vaccine to intestinal endothelial cells with low mammalian toxicity infection through oral enteric-coated capsules, bacteria is phagocytosed through macrophages, and specific molecules COVID-19-S is expressed to be recognized by immune cells, so that the immune effect of specific antibodies in mammals (mice, humans and other mammals) neutralizing COVID-19-S protein is achieved.

The invention provides a detection test strip of salmonella typhimurium. The detection test strip comprises a sample pad, a cellulose nitrate membrane, absorbent paper and a bottom plate. A quantum dot fluorescent micro-sphere is marked with a monoclonal antibody of salmonella typhimurium, and the cellulose nitrate membrane is coated with a detection antibody and a quality control antibody. Basedon the test strip, the invention also provides a method for high-sensitivity and quick quantitative detection of salmonella typhimurium. The method fully utilizes super-strong and stable fluorescent signals of quantum dots, combines the advantages of easy marking and control of polymer sub-micro-spheres, constructs an immunochromatography technology of quantum dot fluorescent micro-spheres, and realizes sensitive and quick detection of salmonella typhimurium.

The invention discloses a primer, a probe and a chip for detecting and identifying a salmonella typhosa nucleic acid segment. The primer sequence comprises a primer pair sequence comprising an upstream primer sequence shown in SEQ ID NO.2 and a downstream primer sequence shown in SEQ ID NO.3. The probe sequence comprises 5'-Zc-P-3', wherein Zc at the 5' end is a sequence complementary to a probe Z fixed on a gene chip, and P at the 3' end is a specific sequence of a salmonella typhosa gene shown in SEQ ID NO.4. The gene chip comprises a probe for detecting and identifying the salmonella typhosa nucleic acid segment and a probe Z fixed on a solid-phase carrier. The primer, the probe and the chip provided by the invention can specifically and purposively detect the salmonella typhosa.

The invention discloses a method for rapidly detecting the concentration of salmonellae in water. The method comprises the following steps of: (1) obtaining a plurality of parts of salmonella solutions with different known concentrations; (2) rapidly detecting the concentration of the salmonellae by using an electrochemical chronoamperometry through a CHI660D electrochemical workstation; determining that a compliance relation between the salmonellae and boron-doped diamond film electrode responding current is a linear relation, wherein parameters of the CHI660D electrochemical workstation are that: a boron-doped diamond film electrode is used as a working electrode, the detection potential is 0.9-1.15V and an electrolytic tank is filled with a PBS (Phosphate Buffer Solution) with the pH (Potential of Hydrogen) being 7.2-7.6; and (3) carrying out sample introduction on a water body to be detected and recording a current value of responding electrophoresis of the boron-doped diamond film electrode; and detecting the salmonella concentration corresponding to the current value by utilizing an obtained responding current-salmonella concentration curve and combining the current value of the sample. The method disclosed by the invention is simple to operate; the detection time is only 1 hour; and the detection limit can reach 1000cfu/mL.

The invention discloses a PCR reaction tube coated with a specific antibody and used for detecting salmonella choleraesuis and an immune PCR detection kit. The PCR reaction tube is pre-coated with the monoclonal antibody which can specifically enrich salmonella choleraesuis in samples. The detection kit can fast, accurately and sensitively detect salmonella choleraesuis from food and other samples, and the sensitivity can reach 103-104 cfu/ml and is increased by 10-100 times than the sensitivity of a conventional PCR. An immunology method and a molecular biology method are organically combined into a whole, enrichment and detection of salmonella choleraesuis in the samples can be achieved in one PCR tube, the operation is simple, the cost is low, the detection is fast, and results are accurate.

The invention relates to a swine cholera attenuated salmonella recombinant strain for expressing a haemophilus parasuis Omp 26 gene. Omp 26 is selected as an exogenous gene, a non-resistant plasmid pYA3493 is selected as a vector, and a swine cholera attenuated salmonella C500 asd gene deletion strain is adopted as a receptor strain to form an Asd+ balanced expression system. The asd gene can be stably inherited under the condition of no exogenous selection pressure, the problem of previous unstable inheritance of exogenous genes can be solved, the system has no marker of any antibiotic gene,a biological safety problem cannot be generated, and foundation is laid for developing the system into a safe, efficient and live vaccine vector; meanwhile, the outer membrane protein Omp 26 of haemophilus parasuis (HPS) can be well expressed and can also serve as special protein for researching clinical detection, epidemiological investigation and immune detection of the HPS. A basic idea is provided for research on bivalent vaccines of swine cholera and haemophilus parasuis.

The invention relates to salmonella broad-spectrum lyase with in-vitro lytic activity and application thereof, and belongs to the technical field of biology. The lyase is named as LysMD19, an amino acid sequence of the lyase is shown as SEQ ID No. 1, and a nucleotide sequence of the lyase is shown as SEQ ID No. 2. The lyase can directly and efficiently lyse salmonella in-vitro under the conditionof no cell permeation reagents such as ETDA, the lysing effect is achieved on salmonella enteritidis, salmonella typhimurium, salmonella pullorum and other salmonella of different serotypes, good temperature stability and high acid and alkali resistance are achieved, the lyase can be used for preventing and treating salmonella infection, and also can be used for killing salmonella in multiple links such as source farms, processing, packaging and transportation to market selling, the safety 'from farms to dining tables' is ensured, and good application prospects and values are achieved.

The application of flagellin derivative CZLC31 from Salmonella in preparation of medicines for preventing and treating inflammatory bowel disease (IBD) is introduced in this invention. The CZLC331 protein has a good preventive and therapeutic effect for IBD, such as ulcerative colitis (UC) and Crohn's disease (CD) in this invention. Therefore, this protein can be prepared into the drug for prevention and treatment of IBD. It has been found that the bad efficacy, slow onset, long course of treatment, side effects can be significantly avoided for existing clinical IBD drugs, it can reduce the patient's pain during the treatment in this invention. The rehabilitation and quality of life of the patients have been largely improved. The drug will play an important role in the prevention and treatment of IBD in this invention, and has broad application prospects.

The invention discloses a water treatment ternary copolymerization bactericide and a preparation method thereof. The preparation method comprises the following steps: weighing glutaraldehyde, AMPS, crylic acid, isopropanol, water, sodium hydroxide, a polymerization inhibitor, sodium hypophosphite, maleic anhydride, ammonium persulfate, a dispersing agent, phosphorus trichloride, N,N-dimethylformamide, L-aspartic acid and glutamic acid according to parts by weight, and uniformly mixing the materials to prepare the bactericide. The scale inhibiting ratio of the bactericide is 85.5-98.5%; the density is 1.15-1.35 g/cm<2>; after the bactericide is stored at 50-60 DEG C for one month, the bactericidal rate is 98.5-99.5%; after the bactericide is stored at 40-50 DEG C for half a year, the bactericidal rate is 97-99%; after the bactericide is stored at the room temperature for 1-2 years, the bactericidal rate is 96-98%; when the bactericide is applied to escherichia coli, staphylococcus aureus, streptococcus albus, gonococcus, penicillium, aspergillus, saprophytic bacteria and salmonella, the bactericidal rate is 98.8-99.8%.

The invention discloses a primer group, a kit and a method for detecting salmonella and listeria monocytogenes, and belongs to the technical field of microbiological detection. The primer group comprises a forward primer mgtC-F and a reverse primer mgtC-R of a mgtC gene of salmonella, and a positive primer prfA-F and a reverse primer prfA-R of a prfA gene of listeria monocytogenes. The invention also provides the kit and the duplex PCR method based on the primer group. The primer group is simple to operate, low in cost, strong in specificity, high in sensitivity, short in detection time and simple in result judgment.

The invention relates to the field of food safety, in particular to a fragrance composition to inhibit harmful microorganisms in foods. The fragrance composition is characterized by comprising, by weight, 5-50% of citral, 10-50% of cinnamaldehyde, 5-50% of linalool, and 1-40% of eugenol. The fragrance composition features safety, naturalness and zero toxicity, can inhibit the food-borne pathogenicbacteria, such as Staphylococcus aureus, Escherichia coli, Salmonella, Shigella, Vibrio parahaemolyticus, Listeria monocytogenes and Bacillus cereus, and can effectively prevent food deterioration and extend the shelf life.