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Preparation method and application of chicken white diarrhea salmonella inactivated vaccine

A Salmonella and inactivated vaccine technology, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, antibacterial drugs, etc., can solve problems such as weak cross-protection, slow immunity, and weak cellular immune protection, and achieve The product is safe and reliable, with good protection ability and high output effect

Active Publication Date: 2015-11-11
JIANGSU INST OF POULTRY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The principle of the present invention is to obtain three strains of different subtypes of Salmonella pullorum with good immunogenicity obtained after years of repeated screening and verification from more than 500 strains of Salmonella pullorum strains isolated from natural cases collected and preserved over the years. The strains SP7220 (intermediate type), SP8441 (mutant type) and SP9905 (standard type) are used as vaccine-making strains. After being cultivated and inactivated, a new adjuvant formula is used to prepare vaccines, which solves the problem of slow immunity of traditional inactivated vaccines. The shortcomings of weak cellular immune protection function and weak cross-protection can comprehensively improve the immune efficacy of vaccines

Method used

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  • Preparation method and application of chicken white diarrhea salmonella inactivated vaccine
  • Preparation method and application of chicken white diarrhea salmonella inactivated vaccine
  • Preparation method and application of chicken white diarrhea salmonella inactivated vaccine

Examples

Experimental program
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Effect test

Embodiment 1

[0021] 1) Separation, purification and staining microscopy

[0022] Salmonella pullorum SP7220 strain was isolated from the liver of Bailaihang chickens that died in a chicken farm in Qingdao City, Shandong Province in November 1972. The specific isolation method is as follows: aseptically collect the liver of the dead chick, place it in nutrient broth for overnight cultivation at 37° C., then inoculate it on Martin agar medium, and cultivate it at 37° C. for 24 hours. Pick a colony with a diameter of 1.0mm-2.0mm, off-white transparent, and smooth surface, inoculate it on Martin agar medium, and incubate at 37°C for 24 hours. The purely cultured bacteria were subjected to Gram staining microscope inspection, and then observed with an optical microscope, showing elongated bacilli with slightly rounded ends.

[0023] Salmonella pullorum sp8441 strain was isolated from the oviduct of 6-month-old white legion laying hens in a chicken farm in Beijing in December 1984. The specifi...

Embodiment 2

[0041] Salmonella pullorum strains SP7220, SP8441 and SP9905 were resuscitated and multiplied respectively, and typical colonies were selected for expansion and culture to make seed bacterial liquid. Inoculate the seed bacterial liquid at 3.0% of the total volume of the culture medium, collect the bacterial liquid after deep aeration culture at 37°C for 12 hours in a fermenter, remove the supernatant by centrifuging at 5000r / min for 15 minutes, and resuspend the bacteria with normal saline containing 0.3% formaldehyde Mud, inactivated at 8°C for 12h. Fully mix the inactivated bacteria solution with an equal volume of adjuvant containing 0.5mg / ml chitosan oligosaccharide, 0.75mg / ml Acanthopanax polysaccharide and 20mg / ml propolis, and aseptically subpackage to make the inactivated Salmonella pullorum vaccine .

[0042] The vaccine was injected intramuscularly into 3-day-old healthy Laihang chickens and 3-day-old healthy Betina turkeys (0.2ml per bird, containing 2 billion bact...

Embodiment 3

[0047] According to the preparation method described in Example 2, the Salmonella pullorum inactivated vaccine was made, and the inactivated vaccine semi-finished product was used at the same time, according to "Practical Veterinary Biological Products Technology" (Zhang Zhenxing, etc., China Agricultural Science and Technology Press, 1996, 53-54) The manufacture method of the oil-emulsion adjuvant inactivated vaccine and the propolis adjuvant inactivated vaccine is respectively made into the oil-emulsion adjuvant inactivated vaccine and the propolis adjuvant inactivated vaccine (the antigen concentration is 10 billion bacteria per milliliter). Eighty 7-day-old healthy Bailaihang chicks were selected and divided into 4 groups. The first group of test chickens was intramuscularly injected with Salmonella pullorum inactivated vaccine (0.2ml per feather) prepared by the preparation method described in Example 1, and the second group was injected intramuscularly with oil emulsion a...

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Abstract

The invention discloses a chicken white diarrhea dyed condensation antigen, a preparation method and an application thereof and belongs to the technical field of veterinarian diagnosis. The antigen is prepared by following steps: performing recovery passage to chicken white diarrhea salmonella strains comprising SP7220, SP7701, SP8441 and SP9905 to obtain a seed bacterial liquid; inoculating the seed bacterial liquid on a solid culture medium for proliferation; inactivating the bacteria in formalin; regulating the concentration of the bacterial liquid through turbidimetry; and finally adding a crystal violet solution to dye the bacterial liquid, fully mixing uniformly the bacterial liquid and packaging the bacterial liquid to obtain the antigen. The preparation method is simple and reasonable, is scientific, is stable in production and is low in cost. The chicken white diarrhea condensation antigen production strain is good in antigenicity and is low in mutation rate. A prepared chicken white diarrhea dyed condensation antigen product is high in sensitivity, is strong in specificity, is quick in diagnosis and is clear in condensation image, and is an excellent dyed condensation antigen for detecting chicken white diarrhea.

Description

technical field [0001] The invention belongs to the technical field of biological products, and in particular relates to a preparation method and application of an inactivated Salmonella pullorum vaccine. Background technique [0002] Pullorum pullorum is a common multiple infectious disease of chickens or turkeys of all ages caused by Salmonella pullorum pullorum. The disease was discovered for the first time in 1899, and it has become prevalent worldwide, causing significant losses to the poultry industry. When chicks less than 20 days old are infected with Salmonella pullorum, acute sepsis usually occurs, and the mortality rate is as high as 80% to 100%. At present, the method of drug control is usually used in China to reduce the harm of the disease, but long-term repeated drug use not only increases the cost of feeding, but also leads to a series of problems such as enhanced bacterial resistance and drug residues. Facts have proved that relying on drug control alone is...

Claims

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Application Information

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IPC IPC(8): A61K39/112A61P31/04
Inventor 龚建森许明张笛苗晋锋朱春红窦新红顾蓓蓓刘学贤徐步童海兵邹剑敏
Owner JIANGSU INST OF POULTRY SCI
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