Culture medium for composite enrichment of salmonella, Listeria monocytogenes and Staphylococcus aureus, and preparation thereof
A technology for Listeria monocytogenes and Salmonella, which is applied in the directions of biochemical equipment and methods, determination/inspection of bacteria and microorganisms, etc., to achieve the effect of shortening the enrichment time
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Embodiment 1
[0022] Weigh tryptone 17g, peptone 3g, sodium dihydrogen phosphate 2.5g, glucose 2.5g, escin 1g, sodium pyruvate 2.5g, mannitol 5g, sodium chloride 20g, lithium chloride 2g, distilled water to 990ml, Heat to dissolve, and after cooling to room temperature, correct the pH value to 7.2-7.3; mix well, and sterilize at 121°C for 15 minutes; weigh 10 mg of nalidixic acid and add it to 5 ml of sodium hydroxide solution with a concentration of 0.05 mol / L to obtain naphthyridine Keto acid solution. Weigh 0.15 mg of potassium tellurite and add it to 5 ml of sterilized distilled water to form a potassium tellurite solution. Under aseptic conditions, add 5ml of nalidixic acid solution and 5ml of potassium tellurite solution; subpackage and store at 4°C for later use, and prepare the compound bacteria-enrichment agent for Salmonella, Listeria monocytogenes and Staphylococcus aureus Medium.
[0023] The prepared culture medium for Salmonella, Listeria monocytogenes and Staphylococcus aur...
Embodiment 2
[0028] Weigh tryptone 17g, peptone 3g, sodium dihydrogen phosphate 2.5g, glucose 2.5g, escin 1g, sodium pyruvate 2.0g, mannitol 10g, sodium chloride 25g, lithium chloride 2.5g, distilled water to 990ml , heated to dissolve, cooled to room temperature and corrected to pH 7.2-7.3; mixed, sterilized at 121°C for 15 minutes; weighed 15mg of nalidixic acid and added to 5ml of sodium hydroxide solution with a concentration of 0.05mol / L to obtain naphthalene Lixonic acid solution. Weigh 0.2 mg of potassium tellurite and add it to 5 ml of sterilized distilled water to form a tellurite solution. Under aseptic conditions, add 5ml of nalidixic acid solution and 5ml of potassium tellurite solution; subpackage and store at 4°C for later use, and prepare the compound bacteria-enrichment agent for Salmonella, Listeria monocytogenes and Staphylococcus aureus Medium.
[0029] The prepared culture medium for Salmonella, Listeria monocytogenes and Staphylococcus aureus complex enrichment were ...
Embodiment 3
[0034] Weigh tryptone 17g, peptone 3g, sodium dihydrogen phosphate 2.5g, glucose 2.5g, escin 1g, sodium pyruvate 1g, mannitol 3g, sodium chloride 10g, lithium chloride 2.5g, distilled water to 990ml, Heat to dissolve, cool to room temperature and correct the pH value to 7.2-7.3; mix well, sterilize at 121°C for 15 minutes; weigh 5 mg of nalidixic acid and add it to 5 ml of 0.05 mol / L sodium hydroxide solution to obtain naphthyridine Keto acid solution. Weigh 0.05 mg of potassium tellurite and add it to 5 ml of sterilized distilled water to form a potassium tellurite solution. Under aseptic conditions, add 5ml of nalidixic acid solution and 5ml of potassium tellurite solution; subpackage and store at 4°C for later use, and prepare the compound bacteria-enrichment agent for Salmonella, Listeria monocytogenes and Staphylococcus aureus Medium.
[0035] The prepared culture medium for Salmonella, Listeria monocytogenes and Staphylococcus aureus complex enrichment were inserted an...
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