A method and application of rapidly capturing Cronobacter based on immunomagnetic bead technology

A technology of Cronobacter and immune magnetic beads, which is applied in the field of food safety, can solve problems such as the long-term enrichment process, and achieve the effects of high sensitivity, shortened detection time, and low cost

Active Publication Date: 2020-07-14
南通春泽营养食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the new detection technology has the advantages of high sensitivity and specificity, it needs a long pre-enrichment process.

Method used

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  • A method and application of rapidly capturing Cronobacter based on immunomagnetic bead technology
  • A method and application of rapidly capturing Cronobacter based on immunomagnetic bead technology
  • A method and application of rapidly capturing Cronobacter based on immunomagnetic bead technology

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1 Materials and methods

[0048] 1.1 Main strains

[0049] The bacterial strains used in this experiment are listed in Table 1 below:

[0050] Table 1 strain information

[0051]

[0052] Standard strains of Cronobacter sakazakii ATCC 29544, ATCC 29004, ATCC 12868, Enterobacter aerogenes ATCC13048, Enterobacter cloacae ATCC 3503, Escherichia coli CMCC B4413, Listeria monocytogenes CMCC 54006 were purchased from the American Type Culture Collection Repository ATCC and China Medical Microbiology Collection Center CMCC.

[0053] 1.2 Experimental method

[0054] 1.2.1 Culture of strains

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Abstract

The invention discloses a method and application of rapidly acquiring Cronobacter on basis of an immunomagnetic bead technology, which belong to the technical field of food safety. The method provided by the invention is characterized by covalent coupling a monoclonal antibody of Cronobacter sakazakii and a carboxyl magnetic bead to acquire an immunomagnetic bead, and then utilizing the acquired immunomagnetic bead to acquire in an acquisition system. According to the method provided by the invention, the Cronobacter sakazakii can be quickly and efficiently acquired and gathered, especially in pure cultures and dairy products, the defects of a traditional pre-bacteria-proliferating process are overcome, the bacteria-proliferating time is greatly shortened, and the method has the advantages of short time consumption, simplicity and convenience in operation, strong specificity, high sensitivity and the like, provides a precondition for realizing rapidly detecting the Cronobacter sakazakii, provides a new pre-processing method for detecting various causative agents at the same time, and can be used for gathering, separating and detecting the Cronobacter.

Description

technical field [0001] The invention relates to a method and application for rapidly capturing Cronobacter based on immunomagnetic bead technology, and belongs to the technical field of food safety. Background technique [0002] Cronobacter sakazakii (formerly Enterobactersakazakii), belonging to the family Enterobacteriaceae, is an oxidase-negative, facultative anaerobic, Gram-negative bacilli with perinatal flagella capable of movement. Cronobacter sakazakii can infect newborns, especially premature infants, with a mortality rate as high as 33% to 80%. Eating food containing Cronobacter can cause necrotizing enterocolitis, sepsis, meningitis and other diseases, with a fatality rate of up to 80%. A large number of studies have shown that Cronobacter infection is one of the most important risk factors for newborns. According to the public records of CDC, FDA and WHO, more than 90% of Cronobacter infections are from infant formula. Therefore, how to quickly and sensitively...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/02C12Q1/10C12R1/01
CPCC12N1/02C12Q1/10
Inventor 满朝新姜毓君周文琦王蕊赵玥明
Owner 南通春泽营养食品有限公司
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