Sample pretreatment methods, kits, and detection methods for antiepileptic drug testing

By using magnetic separation and elution of antiepileptic drugs with magnetic bead suspension, combined with ion mobility spectrometry detection, the problem of long time consumption, complexity and high cost in the detection of antiepileptic drugs in the existing technology is solved, and efficient and accurate sample pretreatment and detection are achieved.

CN121558452BActive Publication Date: 2026-05-26LIANYING YUEZHI SCIENCE INSTRUMENTS (WUHAN) CO LTD
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Patent Information

Application Number
CN202610093870.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2026-01-23
Publication Date
2026-05-26
Estimated Expiration
2046-01-23

AI Technical Summary

Technical Problem

Existing pretreatment methods for antiepileptic drug detection are complex, time-consuming, costly, and difficult to automate, affecting the accuracy and stability of the test results.

Method used

A suspension was prepared by mixing magnetic beads with an activation solution. The supernatant and the magnetic bead complex were separated by magnetic adsorption. Further processing was carried out using rinsing and elution solutions. Combined with ion mobility spectrometry detection, efficient quantitative detection was achieved.

Benefits of technology

It simplifies the operation process, shortens the processing time, reduces costs, and improves the accuracy and stability of the test, making it suitable for high-throughput sample testing and applicable to clinical and research scenarios.

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Abstract

This application relates to the field of analytical detection technology, specifically to sample pretreatment methods, kits, and detection methods for the detection of antiepileptic drugs. The sample pretreatment method for the detection of antiepileptic drugs in this application includes one or more of the following antiepileptic drugs: valproic acid, carbamazepine, phenytoin, levetiracetam, lamotrigine, and phenobarbital. The sample pretreatment method includes the following steps: mixing magnetic beads with an activation solution to prepare a magnetic bead suspension; mixing the sample to be tested, the magnetic bead suspension, and an internal standard solution, and separating the supernatant and the magnetic bead complex after magnetic adsorption treatment; adding an eluent to the magnetic bead complex, and collecting the magnetic bead-target complex after elution treatment; adding an elution buffer to the magnetic bead-target complex, and collecting the supernatant after elution treatment for detection. The above sample pretreatment method is simple to operate, time-saving, and low-cost, providing key technical support for the efficient quantitative detection of antiepileptic drugs.
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