A microbial community preparation device

Abstract

This utility model relates to the technical field of bacterial culture preparation devices, and in particular to a bacterial culture preparation device, comprising: a first filter having a first filter membrane, the first filter input end being connected to a first supply pump; a second filter having a second filter membrane, the second filter input end being connected to the first filter output end; and a third filter having a third filter membrane, the second filter input end being connected to the second filter output end; wherein the diameter of the pores on the first filter membrane is larger than the diameter of the pores on the second filter membrane, and the diameter of the pores on the second filter membrane is larger than the diameter of the pores on the third filter membrane. This utility model is beneficial for removing impurities from bacterial culture samples and enriching the bacterial culture.

Description

Technical Field

[0001] This utility model relates to the field of microbial community preparation device technology, and in particular to a microbial community preparation device. Background Technology

[0002] The microbial community refers to the group of all microorganisms in an ecosystem, which includes a variety of bacteria and other biological factors.

[0003] Microbial samples contain undigested food residue, metabolic waste products from microbial growth and reproduction, parasite eggs, pathogens, and other impurities. Dead bacteria and fragments in these impurities have no therapeutic value and may occupy transplantation space. These components, while lacking therapeutic value, consume valuable transplantation space, compressing the usable space for truly useful live microbiota in practical applications, thus affecting the effectiveness and efficiency of microbial transplantation. Furthermore, harmful components such as parasite eggs and pathogens in impurities severely interfere with and disrupt subsequent microbial culture or enrichment operations. Parasite eggs can hatch under suitable conditions, and pathogens can proliferate rapidly, disrupting the original ecological structure of the microbial community, breaking its internal balance, and consequently reducing the overall survival rate of the microbial community, significantly lowering the quality and effectiveness of microbial preparation. Utility Model Content

[0004] The technical problem to be solved by this utility model is to provide a microbial community preparation device that facilitates the removal of impurities from microbial community samples and the enrichment of microbial communities.

[0005] The technical solution of this utility model to solve the above-mentioned technical problems is as follows:

[0006] A microbial community preparation device, comprising

[0007] A first filter, the first filter having a first filter membrane, and a first supply pump connected to the input end of the first filter;

[0008] A second filter, having a second filter membrane, wherein the input end of the second filter is connected to the output end of the first filter;

[0009] A third filter, wherein the third filter has a third filter membrane, and the input end of the second filter is connected to the output end of the second filter;

[0010] The diameter of the filter pores on the first filter membrane is larger than the diameter of the filter pores on the second filter membrane, and the diameter of the filter pores on the second filter membrane is larger than the diameter of the filter pores on the third filter membrane.

[0011] The beneficial effects of adopting the above scheme are as follows: by filtering in stages through the first, second and third filters, a large number of dead bacteria and debris that have no therapeutic value and occupy transplantation volume are effectively removed, which greatly increases the usable space of truly useful live bacteria in the microbial community sample. This avoids the situation where the number of live bacteria is limited due to impurities occupying valuable volume during the transplantation process, thereby improving the effect and efficiency of microbial community transplantation. At the same time, it is conducive to the rapid screening of target bacteria and subsequent rapid enrichment.

[0012] Based on the above technical solution, the present invention can be further improved as follows.

[0013] Furthermore, the output end of the first filter is connected to the input end of the second filter via a second connecting pipe, and a second supply pump is installed on the second connecting pipe;

[0014] The output end of the second filter is connected to the input end of the third filter through a third connecting pipe, and a third supply pump is installed on the third connecting pipe.

[0015] The beneficial effects of adopting the above-mentioned further scheme are: each supply pump and connecting pipe allows the bacterial sample to pass through each filter stably and evenly in sequence, avoiding problems such as incomplete filtration or local blockage caused by unstable pressure or poor flow, thus improving the reliability and efficiency of filtration.

[0016] Meanwhile, the independent supply pumps can adjust the flow rate and filtration pressure of each filtration stage to adapt to the characteristics and filtration requirements of different filter membranes, further optimize the filtration effect, and ensure that the bacterial samples are fully and effectively filtered step by step.

[0017] Furthermore, the second connecting pipe is a reducing pipe, with the large-diameter end of the second connecting pipe connected to the output end of the first filter and the small-diameter end of the second connecting pipe connected to the input end of the second filter;

[0018] The third connecting pipe is a reducing pipe, with its large-diameter end connected to the output end of the second filter and its small-diameter end connected to the input end of the third filter.

[0019] The beneficial effects of adopting the above-mentioned further solution are: the variable diameter tube helps to increase the flow velocity of the bacterial sample in the connecting tube, enhances the turbulence of the flow, helps to prevent impurities from settling and remaining in the connecting tube, reduces interference with the filtration process, and also helps to improve the transmission efficiency of the bacterial sample between filters, and is also conducive to cooperating with other components to adjust the filtration pressure.

[0020] Furthermore, a first pressure sensor is also provided on the second connecting pipe, and a second pressure sensor is also provided on the third connecting pipe.

[0021] The beneficial effects of adopting the above-mentioned further solution are: the pressure sensor can monitor the pressure changes of the bacterial sample in real time during the filtration process, which is conducive to automatically controlling the start and stop of the second or third supply pump and improving the filtration efficiency of the bacterial sample.

[0022] Furthermore, the first supply pump, the second supply pump, and the third supply pump are all peristaltic pumps.

[0023] The beneficial effects of adopting the above-mentioned further solution are: the peristaltic pump can accurately control the flow rate of the microbial sample, ensure that the filtration process proceeds stably according to the set flow rate, avoid problems such as inconsistent filtration effect or impact on the filter membrane caused by flow fluctuations, and reduce the risk of contamination of the microbial sample, which is conducive to improving the quality and safety of microbial preparation. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the microbial community preparation equipment according to Embodiment 1 of this utility model.

[0025] The attached diagram lists the components represented by each number as follows:

[0026] 1. First filter; 2. First filter membrane; 3. First supply pump; 4. Second filter; 5. Second filter membrane; 6. Third filter; 7. Third filter membrane; 8. Second connecting pipe; 9. Second supply pump; 10. Third connecting pipe; 11. Third supply pump; 12. First pressure sensor; 13. Second pressure sensor. Detailed Implementation

[0027] The principles and features of the present invention are described below with reference to the accompanying drawings. The examples given are only for explaining the present invention and are not intended to limit the scope of the present invention.

[0028] In the description of this invention, it should be understood that the terms "center," "length," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "inner," "outer," "circumferential," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and are not intended to indicate or imply that the system or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0029] In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0030] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances. Example 1

[0031] like Figure 1 As shown, a microbial community preparation device includes...

[0032] The first filter 1 has a first filter membrane 2 and a first supply pump 3 connected to its input end. The first supply pump 3 can be a peristaltic pump, and the input end of the peristaltic pump is connected to the microbial sample storage device through a first connecting tube.

[0033] The second filter 4 has a second filter membrane 5, and the input end of the second filter 4 is connected to the output end of the first filter 1.

[0034] The third filter 6 has a third filter membrane 7, and the input end of the second filter 4 is connected to the output end of the second filter 4.

[0035] The diameter of the filter pores on the first filter membrane 2 is larger than the diameter of the filter pores on the second filter membrane 5, and the diameter of the filter pores on the second filter membrane 5 is larger than the diameter of the filter pores on the third filter membrane 7.

[0036] In this invention, the microbial community sample is filtered step by step through a first filter 1, a second filter 4, and a third filter 6. According to the filtration principle, the smaller the pore size of the filter membrane, the higher the filtration accuracy. This allows impurities in the microbial community sample to be peeled off layer by layer, effectively removing a large number of dead bacteria and debris that have no therapeutic value and occupy transplantation capacity. This greatly increases the usable space for truly useful active bacteria in the microbial community sample, avoiding the situation where the number of active bacteria is limited due to impurities occupying valuable capacity during the transplantation process. This improves the effect and efficiency of microbial community transplantation, and at the same time, it facilitates the rapid screening of target bacteria and subsequent rapid enrichment.

[0037] like Figure 1As shown, in some embodiments, the output end of the first filter 1 is connected to the input end of the second filter 4 via a second connecting pipe 8. A second supply pump 9 is installed on the second connecting pipe 8. The second supply pump 9 can be a peristaltic pump. The output end of the second filter 4 is connected to the input end of the third filter 6 via a third connecting pipe 10. A third supply pump 11 is installed on the third connecting pipe 10. The third supply pump 11 can be a peristaltic pump. The first supply pump 3, the second supply pump 9, and the third supply pump 11 are all electrically connected to a control component (not shown in the figure), which facilitates the control component to automatically control the start and stop of each supply pump. The peristaltic pump squeezes the sample by rotating the rotor in the inner cavity of the tubing. By controlling the rotation speed of the rotor, the flow rate can be precisely adjusted. The sample only contacts the tubing, avoiding direct contact with other components and preventing contamination, thus effectively ensuring the activity and purity of the bacterial sample.

[0038] The second connecting pipe 8 is a reducing pipe. The large-diameter end of the second connecting pipe 8 is connected to the output end of the first filter 1, and the small-diameter end of the second connecting pipe 8 is connected to the input end of the second filter 4. The third connecting pipe 10 is a reducing pipe. The large-diameter end of the third connecting pipe 10 is connected to the output end of the second filter 4, and the small-diameter end of the third connecting pipe 10 is connected to the input end of the third filter 6. The use of reducing pipes increases the flow rate of the bacterial sample in the connecting pipe, creating a turbulent flow state. This is not conducive to the sedimentation and residue of impurities in the pipe, thereby reducing the interference of impurities on the filtration process, improving the transmission efficiency of the bacterial sample between filters, ensuring the filtration effect, and facilitating the control of filtration pressure in conjunction with other components.

[0039] Both the second connecting tube 8 and the third connecting tube 10 can be made of thin-walled silicon tubes, which facilitates deformation of the pure bacterial sample under certain pressure and increases the pressure of the bacterial sample liquid.

[0040] In this embodiment, a first pressure sensor 12 is also provided on the second connecting pipe 8, and a second pressure sensor 13 is also provided on the third connecting pipe 10. Both the first pressure sensor 12 and the second pressure sensor 13 are electrically connected to the control component.

[0041] During microbial filtration, the control component activates the first supply pump 3 to deliver the microbial sample to the first filter 1 for preliminary filtration. The pre-filtered microbial sample then enters the second connecting tube 8, where the first pressure sensor 12 transmits the pressure value of the microbial sample to the control component. Once the pressure of the microbial sample in the second connecting tube 8 reaches a suitable level, the control component activates the second supply pump 9 to deliver the microbial sample from the second connecting tube 8 to the second filter 4 for secondary filtration. The secondary-filtered microbial sample then enters the third connecting tube 10. Once the pressure of the microbial sample in the third connecting tube 10 reaches a suitable level, the control component activates the third supply pump 11 to deliver the microbial sample from the third connecting tube 10 to the third filter 6 for fine filtration, ultimately forming a high-concentration microbial sample with a large number of target microorganisms. Example 2

[0042] The difference between Example 2 and Example 1 lies only in that the third filter 6 is also connected to a sterile collection system. Specifically, the sterile collection system includes a sterile collection bottle and a vacuum pump. The sterile collection bottle has a sealed structure, and its input end is connected to the output end of the third filter 6 via a fourth connecting pipe. A fourth supply pump, which can also be a peristaltic pump, is installed on the fourth connecting pipe and is electrically connected to the control components. The vacuum pump, connected to the sterile collection bottle, can create a negative pressure environment when collecting bacterial samples, accelerating the collection process, reducing the exposure time of the samples to air, and further ensuring the activity and purity of the bacterial samples. Example 3

[0043] The difference between Example 3 and Examples 1 and 2 is that a sample pretreatment component, including a stirring device and a heating device, is also installed between the first supply pump 3 and the bacterial sample storage container. The stirring device consists of a motor-driven stirring paddle, which can fully stir the bacterial sample, making the bacterial distribution more uniform and avoiding the impact of sedimentation or stratification of the bacterial population during storage on the subsequent filtration effect.

[0044] The heating device uses heating wires wrapped around the outer wall of the connecting tube, which can moderately heat the bacterial sample when needed. Some bacterial groups are more active at suitable temperatures, which is more conducive to subsequent filtration operations. At the same time, heating can also break down the structure of some impurities, making them easier to filter out. This pretreatment component is electrically connected to the control component, which can preset parameters such as stirring time and heating temperature to achieve automatic control.

[0045] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0046] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A bacterial flora preparation apparatus, characterized by: include A first filter (1) has a first filter membrane (2) and a first supply pump (3) is connected to the input end of the first filter (1). The second filter (4) has a second filter membrane (5), and the input end of the second filter (4) is connected to the output end of the first filter (1); The third filter (6) has a third filter membrane (7), and the input end of the second filter (4) is connected to the output end of the second filter (4); The diameter of the filter holes on the first filter membrane (2) is larger than the diameter of the filter holes on the second filter membrane (5), and the diameter of the filter holes on the second filter membrane (5) is larger than the diameter of the filter holes on the third filter membrane (7).

2. The flora preparation device according to claim 1, characterized by: The output end of the first filter (1) is connected to the input end of the second filter (4) through a second connecting pipe (8), and a second supply pump (9) is installed on the second connecting pipe (8). The output end of the second filter (4) is connected to the input end of the third filter (6) through a third connecting pipe (10), and a third supply pump (11) is installed on the third connecting pipe (10).

3. The microbiota preparation device of claim 2, wherein: The second connecting pipe (8) is a reducing pipe. The large diameter end of the second connecting pipe (8) is connected to the output end of the first filter (1), and the small diameter end of the second connecting pipe (8) is connected to the input end of the second filter (4). The third connecting pipe (10) is a variable diameter pipe. The large diameter end of the third connecting pipe (10) is connected to the output end of the second filter (4), and the small diameter end of the third connecting pipe (10) is connected to the input end of the third filter (6).

4. The flora preparation apparatus according to claim 2, characterized by: The second connecting pipe (8) is also provided with a first pressure sensor (12), and the third connecting pipe (10) is also provided with a second pressure sensor (13).

5. The microbial community preparation equipment according to claim 2, characterized in that: The first supply pump (3), the second supply pump (9) and the third supply pump (11) are all peristaltic pumps.

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