Qualitative and quantitative detection method of bacterial drug resistance protein arma

Abstract

The invention relates to a qualitative and quantitative detection method of a bacteria resistant protein ArmA. The method comprises the following steps: firstly extracting the protein in a sample to be detected, thus obtaining the sample protein; carrying out enzyme digestion on the sample protein; then detecting the ArmA specific peptide fragments by adopting membrane electrospray ionization mass spectrometry, qualitatively and quantitatively judging the existence of the ArmA gene on the peptide fragment level, wherein the ArmA specific peptide fragments are three specific peptide fragments and the sequences of the specific peptide fragments are respectively shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The method has the beneficial effects that the matrix effects caused by introduction of small molecules during enzyme digestion are effectively removed by adopting membrane electrospray ionization mass spectrometry and the desalination step in the conventional mass spectrometry process is omitted, so that the method can achieve rapid detection under the condition of extremely high sensitivity.

Description

technical field [0001] The invention relates to the application of membrane electrospray ionization mass spectrometry, in particular to a method for qualitatively and quantitatively detecting bacterial drug-resistant protein ArmA by using membrane electrospray ionization mass spectrometry. Background technique [0002] The emergence and spread of bacterial resistance has become an important public health problem worldwide. [1] Bacteria rely on mobile elements such as inserts, plasmids, and "drug-resistant genomic islands" to rapidly acquire antibiotic resistance. [2] At present, although more than 15 kinds of antibiotics designed for different physiological or metabolic functions of bacteria are widely used, none of them are spared from various resistance mechanisms of bacteria. [3] In the face of each antibiotic, bacteria have evolved coping strategies, and the resistance mechanism is related to a series of resistance genes. For example, bacteria acquire qnrA / B / S genes to...

AI Technical Summary

Problems solved by technology

[0005] However, the current methods are mostly complex sample pretreatment methods, which cannot meet the rapid qualitative and quantitative detection of bacterial drug resistance under the premise of ensuring high sensitivity.

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