Enrichment culture medium and detection method for metamorphic bacteria in fermented wine aging process

A detection method and enrichment culture technology are applied in the enrichment medium and detection field of spoilage bacteria during the aging process of fermented wine, which can solve the problems of long time consumption, shorten the time consumption, shorten the time consumption of enrichment, and ensure the sensory flavor. Effect

Pending Publication Date: 2021-09-28
海天醋业集团有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the medium involved in this technical solution still take

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  • Enrichment culture medium and detection method for metamorphic bacteria in fermented wine aging process

Examples

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Example Embodiment

[0057] In a second aspect, the present invention provides a method of preparing a medium as described above, the preparation method comprising the step of: a supernatant of the fermentation wine concentrate, the MRS medium, and water mixing.

[0058] In one of the examples, the preparation of the supernatant of the fermentation wine concentrate comprises the step of concentrating the unveiled fermentation wine, collecting the concentrate; centrifugation of the concentrate, collecting the supernatant.

[0059] In one of the examples, the temperature used in the concentration is 40 ° C to 50 ° C. It will be appreciated that in order to facilitate storage and subsequent use, the concentrated concentrate seal can be stored in a low temperature environment (e.g., 4 ° C). The invention is not particularly limited, including, but not limited to, concentration using a rotary evaporator using a rotary evaporator.

[0060] In one of the examples, the conditions of centrifugation include: ce...

Example Embodiment

[0083] Example 1:

[0084] Step 1, the preparation of the fermented wine concentrate: Take normal yellow wine 500 mL, and the rotary evaporator was controlled to 250 mL, 4 ° C, 5000 r / min, centrifuge for 5 min, and taking the supernatant.

[0085] Step 2, the medium preparation: Take the above concentrate 50 mL, MRS medium 5.4 g, and dissolve mixed to 100 ml of the distilled water, take 8 ml of 8 mm of 18 mm × 180 mm test tube, 30 min, cool spare.

[0086] Step 3, inoculation culture: Test tube inoculated 1 mL to be tested to test the yellow wine sample, 36 ° C constant temperature culture for 12 h.

[0087] Synchronization will be tested to be tested for MRS tablet detection.

[0088] Step 4, microbial collection: The microorganisms in the above medium were collected using a frozen centrifuge (5000 r / min, 4 ° C), and the supernatant was collected, and after the supernatant was used to use 2 ml of sterilized PBS solution (ie PBS buffer) cleaning 3. Substation (5000 r / min, 5 m...

Example

[0093] Contrast example one:

[0094] Step 1, the medium preparation: Take the MRS medium 5.4 g, dissolve mixed with distillation water to 100 ml, dispensed into a test tube of 18 mm × 180 mm, each test tube is filled with 8 ml, 121 ° C high pressure steam sterilization for 30 min, cooling spare;

[0095] Step 2, inoculation training: Each test tube is inoculated with 1 ml to test the yellow wine sample, and synchronously set the control group, and will test the yellow wine sample to be tested for 30 min for 30 min, respectively, to test the yellow wine sample and sterilization. Wine and wine sample 36 ° C constant temperature culture 12h.

[0096] Synchronize the sample to be tested for MRS tablet detection.

[0097] Step 3: Microbial Collection: The microorganisms in the above medium were collected using a frozen centrifuge (5000 r / min, 4 ° C), and after the supernatant was removed, 2 ml of sterilized PBS solution was cleaned 3 times (5000R / min, 5 min, 4 ° C), obtained a mi...

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Abstract

The invention relates to an enrichment culture medium and a detection method for metamorphic bacteria in a fermented wine aging process. The culture medium comprises a supernatant of a fermented wine concentrated solution and an MRS culture medium, wherein the fermented wine concentrated solution is a concentrated solution of non-metamorphic fermented wine. The culture medium can be used for culturing microorganisms contained in to-be-detected fermented wine, can obviously promote microorganism breeding, shortens microorganism enrichment time, and is convenient for rapidly preparing a microorganism sample for detection. For example, compared with the traditional technology that the enrichment culture time is at least two days, the enrichment culture time of the microorganisms contained in the fermented wine is obviously shortened. Moreover, microorganisms are detected through an ATP detection method, whether metamorphic bacteria are bred or not is judged according to the result, the whole detection period can be completed within 15-18 h, and compared with traditional MRS plate detection, the detection period is shortened to 15-18 h from 72 h, the risk that the fermented wine goes bad in the detection period is avoided, and sterilization treatment can be conducted on the fermented wine in advance.

Description

technical field [0001] The invention relates to the technical field of microbial monitoring of fermented wine, in particular to an enrichment medium and a detection method for spoilage bacteria in the aging process of fermented wine. Background technique [0002] During the production and aging process of traditional brewed wine, there will be fluctuations in the quality of fermented wine, which may rise in acidity and return to turbidity. However, there is a certain lag in the detection of microorganisms and physical and chemical indicators of spoilage bacteria in fermented wine. The changes in total acid and pH can only be detected when the rancidity microorganisms in fermented wine multiply and produce metabolites. Due to timely sterilization, the quality of fermented wine often fluctuates during the detection period. At the same time, if only the turbidity is monitored, the chemical return to turbidity in the fermented wine cannot be ruled out. Therefore, in order to ens...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
CPCC12Q1/045C12Q2304/60
Inventor 宋开阔邢国辉白永平刘源赵艳军
Owner 海天醋业集团有限公司
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