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Preparation for producing COVID-19 antibody after oral administration and preparation method of preparation

A coronavirus, production method technology, applied in the biological field, can solve problems such as the inability to effectively prevent the second, third and other infections

Pending Publication Date: 2020-08-14
内蒙古自治区国际蒙医医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] There is currently no vaccine for the new coronavirus COVID-19 on the market. The current method of preventing infection is isolation, which cannot effectively prevent the outbreak of the second or third infection

Method used

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  • Preparation for producing COVID-19 antibody after oral administration and preparation method of preparation
  • Preparation for producing COVID-19 antibody after oral administration and preparation method of preparation
  • Preparation for producing COVID-19 antibody after oral administration and preparation method of preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Use primer P1, such as SEQ No.1

[0021] (CGCTAGACTGTTCTTATTGTTAACACAAGGGAGAAGAGCATATGAATATCTCTCCTTAG) and primer P2

[0022] (GCAGCGACAGCGGCAGAAAATGGCGAGCAAATTTATTCAGTGTAGGCTGGAGCTGCTTC) is carried out PCR with pKD3 as template, obtains the PCR fragment that is 1091bp in length ( figure 1 ). Purification was carried out with Tiangen Biochemical Technology (Beijing) Co., Ltd. General Product Purification Kit (Product No. DP204), and the DNA concentration was measured with a NanoDrop2000 spectrophotometer.

[0023] Wild-type Salmonella typhimurium (Salmonella typhimurium, LT2) with pKD46 plasmid was cultivated in 1 mL of LB. Culture medium plus ampicillin with a final concentration of 50 μg / mL was cultured overnight at 30° C. on a shaker at 150 r / min. The next day, the overnight culture product was inoculated 1:100 in 50 mL of SOB medium with a final concentration of 10 mM arabinose and 50 μg / mL of ampicillin, and then cultured on a shaker at 30 °C at 150 r / min for...

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Abstract

The invention discloses a preparation for producing a COVID-19 antibody after oral administration and a preparation method of the preparation. Attenuated salmonella typhimurium is used as a carrier todeliver DNA vaccine, the preparation effectively reaches intestinal tract and delivers DNA vaccine to intestinal endothelial cells with low mammalian toxicity infection through oral enteric-coated capsules, bacteria is phagocytosed through macrophages, and specific molecules COVID-19-S is expressed to be recognized by immune cells, so that the immune effect of specific antibodies in mammals (mice, humans and other mammals) neutralizing COVID-19-S protein is achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation for producing novel coronavirus antibodies after oral administration and a preparation method thereof. Background technique [0002] At present, there is no vaccine for the new coronavirus COVID-19 on the market. The current method of preventing infection is isolation, which cannot effectively prevent the outbreak of the second or third infection. Vaccines are the only tool that can prevent large-scale outbreaks. Contents of the invention [0003] In order to solve the above problems, the present invention provides a preparation for producing novel coronavirus antibodies after oral administration and a preparation method thereof. The present invention uses attenuated Salmonella as a carrier to deliver the DNA vaccine, and effectively reaches the intestinal tract with low-toxic infection through oral enteric capsules. The DNA vaccine is sent to the intesti...

Claims

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Application Information

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IPC IPC(8): A61K39/215A61K9/48A61K47/10A61P31/14C12N15/74C12N15/50C12N1/21C12R1/42
CPCA61K39/12A61P31/14A61K9/4858C12N15/74C07K14/005A61K2039/523A61K2039/522A61K2039/53A61K2039/542C12N2770/20022C12N2770/20051C12N2770/20034
Inventor 杨德志毕力格特格喜白音特木其乐陈沙娜斯钦
Owner 内蒙古自治区国际蒙医医院
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