Fluorescent immunochromatography detection method and detection test paper of salmonella typhimurium

A fluorescence immunochromatography, Salmonella technology, applied in measurement devices, analytical materials, instruments, etc., can solve the problem of low sensitivity of Salmonella typhimurium, and achieve the effect of easy popularization, good reproducibility and high sensitivity detection

Inactive Publication Date: 2018-06-29
WUHAN ACADEMY OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] In view of this, the present invention provides a fluorescent immunochromatographic detection method and detection test paper for Salmonella typhimurium, which can quickly and sensitively detect Salmonella typhimurium in food, so as to overcome the shortcomings of the low sensitivity of the existing immunochromatographic method for detecting Salmonella typhimurium

Method used

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  • Fluorescent immunochromatography detection method and detection test paper of salmonella typhimurium
  • Fluorescent immunochromatography detection method and detection test paper of salmonella typhimurium
  • Fluorescent immunochromatography detection method and detection test paper of salmonella typhimurium

Examples

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Embodiment 1

[0041] This embodiment provides a test paper for detection of Salmonella typhimurium, comprising a bottom plate, on which a sample pad, a nitrocellulose membrane and an absorbent paper are sequentially arranged on the bottom plate, and the sample pad is lapped on the upper surface of one end of the nitrocellulose membrane, The absorbent paper is connected to the upper surface of the other end of the nitrocellulose membrane, and the nitrocellulose membrane is coated with a detection line and a quality control line separated from each other, and the distance between the detection line and the quality control line is 9mm. The detection line is Salmonella typhimurium antibody, and the quality control line is goat anti-mouse secondary antibody. The schematic diagram of the assembly and detection of the test strip is as attached figure 1 shown.

[0042] Based on the above-mentioned Salmonella typhimurium detection test paper, this embodiment also provides a fluorescent immunochroma...

Embodiment 2

[0053] In this embodiment, the reproducibility and accuracy of the fluorescent immunochromatographic detection method for Salmonella typhimurium provided in Example 1 are tested, and the specific process includes:

[0054] 1. Prepare different concentrations of Salmonella typhimurium solutions. The concentrations of Salmonella typhimurium in each solution are 0 CFU / mL, 5×10 4 CFU / mL, 10 5 CFU / mL, 10 6 CFU / mL, 10 7 CFU / mL.

[0055] 2. Take 10 samples of each concentration solution, and use the method described in Example 1 for detection, and the detection results are shown in Table 1.

[0056] Table 1. Experimental results of reproducibility and accuracy of Salmonella typhimurium test strips

[0057]

[0058] It can be seen from Table 1 that the detection rate of Salmonella typhimurium samples at different concentrations is 100%, the detection results of all samples are accurate, and the reproducibility and accuracy are extremely high.

Embodiment 3

[0060] The present embodiment provides a fluorescent immunochromatographic detection method of Salmonella typhimurium, the test paper it adopts is the same as that in Example 1, and the steps of the detection method include:

[0061] 1. Preparation of quantum dot fluorescent microspheres

[0062] Take 1 mL polystyrene microspheres (20 mg in weight), add 380 μL CdSe / ZnS quantum dots, place on a shaker at room temperature at 150 rpm for 1.5 h, centrifuge (12000 rpm × 20 min), add ultrapure water for 5 times, and then add 1 mL Polyethyleneimine solution (molecular weight is 750K, concentration is 16mg / mL), after ultrasonic dispersion, put it on a shaker at room temperature at 150rpm for 2h, then centrifuge (12000rpm×20min), wash with ultrapure water for 3 times, and then wash with anhydrous After centrifuging and washing with ethanol for 3 times, redisperse with polyvinylpyrrolidone in ethanol (20 mg / mL). After reacting on a shaking table at room temperature for 24 hours, add 10...

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Abstract

The invention provides a detection test strip of salmonella typhimurium. The detection test strip comprises a sample pad, a cellulose nitrate membrane, absorbent paper and a bottom plate. A quantum dot fluorescent micro-sphere is marked with a monoclonal antibody of salmonella typhimurium, and the cellulose nitrate membrane is coated with a detection antibody and a quality control antibody. Basedon the test strip, the invention also provides a method for high-sensitivity and quick quantitative detection of salmonella typhimurium. The method fully utilizes super-strong and stable fluorescent signals of quantum dots, combines the advantages of easy marking and control of polymer sub-micro-spheres, constructs an immunochromatography technology of quantum dot fluorescent micro-spheres, and realizes sensitive and quick detection of salmonella typhimurium.

Description

technical field [0001] The invention relates to the technical field of rapid detection of food safety, in particular to a fluorescent immunochromatographic detection method of Salmonella typhimurium and a detection test paper. The method can sensitively and rapidly quantitatively detect Salmonella typhimurium in food. Background technique [0002] Foodborne pathogens have been a serious threat to human health and property safety. According to statistics, in bacterial food poisoning in various countries in the world, food poisoning caused by Salmonella often ranks first. Salmonella is also the first in my country's inland areas. The communiqués of the National Health and Family Planning Commission of China show that Salmonella has consistently ranked first among various pathogenic bacteria that cause food poisoning for many years. The Global Foodborne Infections Network (Global Foodborne Infections Network) , GFN) estimates that there are 94 million non-typhoidal Salmonella i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 胡姣
Owner WUHAN ACADEMY OF AGRI SCI
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