115 results about "Salmonella ser. Typhimurium" patented technology

The present invention is directed to the isolation and use of super-infective, tumor-specific vectors that are strains of parasites including, but not limited to bacteria, fungi and protists. In certain embodiments the parasites include, but are not limited to, the bacterium Salmonella spp., such as Salmonella typhimurium, the bacterium Mycobacterium avium and the protozoan Leishmania amazonensis. In other embodiments, the present invention is concerned with the isolation of super-infective, tumor-specific, suicide gene-containing strains of parasites for use in treatment of solid tumors.

The invention discloses a method for simultaneously and quickly detecting salmonella typhimurium, escherichia coli O157:H7 and listeria monocytogenesis in food. The method comprises the following steps of: preparing nano immunomagnetic particles of the targeted typhimurium, escherichia coli O157:H7 and listeria monocytogenesis so as to capture target bacteria from a detection sample; obtaining the target bacteria by magnetic field separation; treating by adopting propylmercuric bromide azide (PMA) to eliminate the interference of killed bacteria; and finally, extracting DNA (Deoxyribose Nucleic Acid) to carry out multiple PCR (Polymerase Chain Reaction) detection. Compared with a classical bacteria separation and identifying and serological typing method, the method disclosed by the invention is quick and accurate, can be used for only detecting viable bacteria and can be used for simultaneously detecting and identifying the three pathogens.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum. Further, the present invention relates to a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella Enteritidis or Salmonella Typhimurium, Fowl Typhoid caused by Salmonella Gallinarum, and Pullorum disease caused by Salmonella Pullorum, comprising the bacteriophage as an active ingredient. Furthermore, the present invention relates to a feed additive, drinking water, a cleaner and a sanitizer, comprising the bacteriophage as an active ingredient.

The invention relates to a method for detecting bacteria. The method comprises the steps as follows: coupling magnetic nanospheres and fluorescent nanospheres with mouse typhus salmonella antibodies so as to obtain mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres, and adding the mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres into a detection system so as to realize magnetic capture and fluorescent labeling to the mouse typhus salmonella simultaneously. About 10 CFU / mL of the mouse typhus salmonella can be detected through observation of a fluorescent microscope; and a good quantitative relation is obtained through detection of a fluorescence spectrophotometer, the linear range is 105-107 CFU / mL, and R2 is equal to 0.9994. The whole detection process is very simple, high in sensitivity and strong in specificity, and can be finished detection within 1.5 h.

The invention provides a visual detection method for mouse typhus salmonella based on aptamer recognition. The basic principle of the method is that the surface of a microwell plate is coated with avidin, sulfhydrylation salmonella aptamer which is marked by biotinylation salmonella aptamer and nanogold is adopted to recognize targeted salmonella, and on the basis, a silver enhancement technique is utilized to further amplify a detection signal, so that the visual detection is achieved. The visual detection method for the mouse typhus salmonella based on the aptamer recognition has the advantages of high specificity, strong sensitivity, simplification and rapidness, thereby providing the possibility for rapidly detecting the mouse typhus salmonella in an environment.

The invention discloses a bacteriophage capable of controlling Salmonella typhimurium; the bacteriophage is classified and named Salmonella typhimuriumphage Phi Sa-1, and is already collected in China Center for Type Culture Collection on 28 April, 2017, under CCTCC M2017217 in Wuhan University, China. The bacteriophage of the invention has specific killing activity for Salmonella typhimurium, and is capable of selectively killing specific pathogenic bacteria without killing beneficial bacteria or causing drug resistance. The bacteriophage also has excellent acid tolerance and heat resistance, may be prepared into feed additives, drinking water additives, feeds, drinking water, detergents or sanitizers and other various products, and is capable of killing Salmonella typhimurium under wide temperature range and pH range.

The invention relates to an aptamer based salmonella typhimurium detection biosensor and a preparation method thereof. Signal amplification is realized by continuous generation and cyclic utilization of Primer, and consequently high-sensitivity detection of salmonella typhimurium is realized, and lower detection limit can be acquired. The aptamer based salmonella typhimurium detection biosensor is high in specificity and sensitivity, low in cost and quick in detection. By the detection method, a salmonella typhimurium detection limit is 5-15cfu / mL, and a detection range is 1*10-1*107cfu / mL.

The invention discloses pseudomonas aeruginosa bacteriophage endolysins, and a coding gene and application thereof. The invention provides a protein which is as shown in (a) or (b) as below: (a) the protein which is as shown in a sequence 1 in a sequence table and consists of an amino acid sequence; (b) the protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence which is as shown in the sequence 1 in the sequence table, has a function of cracking gram-negative bacteria and is deviated from the sequence 1 in the sequencetable. The protein provided by the invention can crack a plurality of gram-negative bacteria, such as Escherichia coli, Klebsiella pneumonia, pseudomonas aeruginosa, Burkholderia cepacia and Salmonella typhimurium. The protein and a surfactant EDTA (Ethylene Diamine Tetraacetic Acid) act synergistically to achieve a stronger inhibiting effect on the gram-negative bacteria. A foundation is laid for researching and developing a new antibacterial preparation.

The invention provides a salmonella broad-spectrum virulent bacteriophage. The preservation number of the bacteriophage is CCTCC NO: M 2020839. The invention also provides a preparation process and application of the salmonella broad-spectrum virulent bacteriophage, and the preparation process comprises fermentation and extraction; in the step of fermentation, a salmonella gallinarum attenuated vaccine strain is used as host bacteria for bacteriophage fermentation. The bacteriophage XPARCPS02 disclosed by the invention can be used for cracking salmonella of various serotypes and also has different degrees of cracking effects on drug-resistant bacteria; the salmonella typhimurium lysate lysate has a cracking capability on salmonella typhimurium, salmonella typhimurium drug-resistant bacteria, salmonella gallinarum, salmonella enteritidis, salmonella enteritidis drug-resistant bacteria, salmonella typhimurium, salmonella pullorum, salmonella paratyphi A, salmonella paratyphi B and salmonella dubicularis.

The invention provides Salmonella typimurium S496. The Salmonella typimurium S496 is characterized by lacking an rfbB gene and an rffG gene; a pagL gene is replaced by an arabinose-regulatory-sequence-containing rfbB gene with a changed SD sequence and a changed initiation codon, and the nucleotide sequence of the arabinose-regulatory-sequence-containing rfbB gene with the changed SD sequence and the changed initiation codon is shown as SEQ ID No.1; the classification of the attenuation strain is named as the Salmonella typimurium S496, the Salmonella typimurium S496 is collected in the China Center For Type Culture Collection (CCTCC), the collection number is CCTCC M2015562, and the collection time is September 21st, 2015. The invention further provides a method for constructing the Salmonella typimurium S496. Meanwhile, the invention provides an application of the strain or the attenuation strain constructed with the construction method in vaccine.

The invention discloses medical organic silicon quaternary ammonium salt antibacterial agent modified PP and a preparation method thereof. The modified PP is prepared through the following steps: weighing the following components: PP, zinc stearate, an organic silicon quaternary ammonium salt antibacterial agent, sodium benzoate, LDPE, calcium carbonate, an antioxidant, phosphite ester, polybutadiene, barium stearate, an ultraviolet ray absorbent, calcium stearate, a styrene thermoplasticity elastomer, CPE and carbon black in parts by weight, uniformly mixing the weighed components, and extruding and pelleting the uniformly-mixed components so as to obtain the modified PP. The PP has the advantages that the impact strength is 3.5-5.5 kJ / m<2>, elasticity modulus is 2-4 GPa, the antibacterial rate for escherichia coli is 85-95%, the antibacterial rate for golden staphylococcus is 95-99%, the bacteriostasis rate for pseudomonas aeruginosa is 97.5-99.5%, the bacteriostasis rate for salmonella typhimurium and Klebsiella pneumoniae is 92-96%, and the bactericidal rate for sarcina is 90-95%.

The invention discloses an LAMP primer combination for detecting 8 environmental pathogens of dairy cow mastitis and application thereof. The primer combination provided by the invention is composed of 48 primers shown in sequence 1 to sequence 48. The primer combination provided by the invention can be used for detecting whether a to-be-detected bacterium is Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus dysgalactiae, Streptococcus uberis, Salmonella typhimurium, Proteus mirabilis or Candida albicans, and can be used for detecting whether a to-be-detected sample contains Escherichia coli and / or Klebsiella pneumonia and / or Pseudomonas aeruginosa and / or Streptococcus dysgalactiae and / or Streptococcus uberis and / or Salmonella typhimurium and / or Proteus mirabilis and / or Candida albicans. The primer combination provided by the invention is used for identifying and detecting the 8 environmental pathogens of dairy cow mastitis, has high specificity and high sensitivity, and can realize simple, rapid and accurate detection, thus having great popularization value.

The invention discloses a biosensor for detecting salmonella typhimurium and application of the biosensor, and belongs to the technical field of harmful microorganism detection. The biosensor is composed of carbon quantum dots Td-CD modified by a salmonella typhimurium nucleic acid aptamer and magnetic beads Td-MB modified by the salmonella typhimurium nucleic acid aptamer. The salmonella typhimurium nucleic acid aptamer and other nucleic acids are assembled through sequence design to form a DNA regular tetrahedron structure, and then the DNA regular tetrahedron structure is respectively usedfor modifying carbon quantum dots and magnetic beads. The method for detecting salmonella typhimurium by adopting the biosensor is simple and convenient to operate and short in consumed time, complexpretreatment does not need to be carried out on a sample to be detected, and the detection cost is low; the biosensor can effectively amplify signals of salmonella typhimurium, can be used for detecting low-concentration salmonella typhimurium in food, and achieves qualitative and quantitative analysis of salmonella typhimurium.

The invention discloses a salmonella typhimurium bacteriophage T156 and application thereof. The bacteriophage is a broad-spectrum type, can split salmonella and a drug-resistant strain thereof, and is identified as a caudal bacteriophage long-tail bacteriophage family; and the bacteriophage T156 has stable titer with the pH of 3-12 and the temperature of 30-50 DEG C. The bacteriophage provided bythe invention can be used for effectively controlling salmonella typhimurium ATCC 13311 in a food sample, and has the characteristics of high specificity, no residue and safety compared with antibiotics and chemical preservatives.

The invention discloses a medical PP inorganic antibacterial composite and a preparation method thereof. The medical PP inorganic antibacterial composite is prepared by use of the steps of weighing PP, divinyl benzene, a nano-zirconium phosphate silver-loaded antibacterial agent, an AC foaming agent, calcium stearate, PE, an antioxidant, paraffin, dicumyl peroxide, titanium dioxide, a surface treating agent, thiodipropionic acid dilauryl ester, azodicarbonamide, sodium benzoate and a titanate coupling agent in parts by weight, mixing all the components evenly and then extruding and pelletizing. The relative density of the obtained composite is 0.05-0.45 and the tensile strength of the composite is 2.5-4.5MPa; besides, the composite has the antibacterial rate of 99.5-99.9% to escherichia coli, 99.5-99.9% to staphylococcus aureus and 99-99.5% to pseudomonas aeruginosa, the bending strength of 11-15MPa, the degree of crosslinking of 45-55%, the impact strength of 3-5kJ / m<2>, and the bacteriostasis rate of 92-96% to salmonella typhimurium and Klebsiella pneumonia.

In one aspect, methods and kits for determining the mutagenic potential of a test substance. The method includes exposing a tester strain (such as Salmonella typhimurium) to the substance, wherein the tester strain includes a gene (such as the histidine gene) having a preexisting mutation conferring auxotrophy, and the mutation is located at a pre-determined position in the gene, growing the tester strain in growth media lacking histidine, and detecting the presence of a back-mutation at the position by analysis of the nucleic acid. The tester strain can be selected from TA98, TA100, TA102 TA1535, TA1537, TA1538, and TA97. The test substance can be any of a wide variety of compounds such as petroleum extracts, pesticides, cosmetics, adhesives, herbicides, hair dyes, and pharmaceuticals. The detecting step can include one or more conventional mutation detection methods. Also provided are kits for conducting the method. The kits can include one or more tester strains, PCR primers, positive control compounds, and DNA polymerase.

This invention relates to preparation and application of orally administered recombinant DNA vaccine for accelerating animal growth, which Salmonella typhimurium CSO22 / pGMCSF-SS (CCTCC M206141) is containing fusion expression plasmid pGMCSF-SS. Salmonella typhimurium CSO22 / pGMCSF-SS is prepared by: cloning GMCSF, fusing with plasmid pcS / 2SS containing somatostatin gene to obtain fusion expression plasmid pGMCSF-SS, and transforming Salmonella typhimurium. This invention also discloses the application of Salmonella typhimurium CSO22 / pGMCSF-SS in orally administered recombinant DNA vaccine for accelerating animal growth.

The invention belongs to the field of microbiological detection and discloses a method for detecting live salmonella typhimurium, salmonella paratyphi b and salmonella typhi in food simultaneously and rapidly. A special primer is designed aiming at salmonella typhimurium, salmonella paratyphi b and salmonella typhi, hydrazoic brominated c ingot processing is adopted to remove interference of dead bacteria, and finally deoxyribonucleic acids (DNAs) are extracted to perform multiplex polymerase chain reaction. Compared with a classical bacterium separating and identifying method and a serologic typing method, the method for detecting live salmonella typhimurium, salmonella paratyphi b and salmonella typhi in the food simultaneously and rapidly is rapid and accurate.

The invention discloses a recombination attenuated salmonella typhimurium carrier vaccine of expression IBDV (Infectious Bursal Disease Virus) immunogenic gene, a preparation method and application thereof, in particular relates to oral attenuated salmonella typhimurium. The recombination attenuated salmonella typhimurium comprises a sequence disclosed by SEQ ID NO.1, and the recombination virus can express IBDV capsid protein VP2. The preparation method comprises the following steps of: inoculating the attenuated salmonella typhimurium in an LB substrate for culturing 18 hours; regulating bacterial concentration as 1010CFU / ml; and preparing the safe and effective oral attenuated salmonella typhimurium carrier vaccine for preventing IBD (Infectious Bursal Diseases).

Use of flagellins from the genus Marinobacter as vaccine adjuvants. This invention is based on the use of two recombinant flagellins, F and FR, from the species Marinobacter algicola (DG893T strain), as vaccine adjuvants capable of developing a specific immune response, against peptides or proteins fused to said flagellins, or administered unfused jointly with the flagellins. This invention also describes new combined vaccination strategies, based on the two Marinobacter algicola flagellins and the Salmonella typhimurium flagellin.

The invention discloses a medical silver-carrying antibacterial agent modified PP material and a preparation method thereof. The PP material is prepared through the following steps: weighing the components of PP, EPDM, a nanometer silver-carrying zirconium phosphate antibacterial agent, barium stearate, acetone, talcum powder, an antioxidant, titanium dioxide, dicumyl peroxide, butadiene rubber, a surface treating agent, lead stearate, propylene, zinc oxide and 5-ethylidene-2-norbornene in parts by weight, uniformly mixing the weighed components, and extruding and pelleting the uniformly-mixed components so as to obtain the PP material. The PP material has the advantages that the breaking elongation rate is 15-55%, the tensile strength is 28-30 MPa, the antibacterial rate for escherichia coli is 98-99%, the antibacterial rate for golden staphylococcus is 97.5-99.5%, the impact strength is 3-5 kJ / m<2>, the bactericidal rate for pseudomonas aeruginosa is 99-99.5%, and the bacteriostasis rate for salmonella typhimurium and Klebsiella pneumoniae is 92-96%.

The present invention relates to the construction and application of engineering bacterium LZ-17, and belongs to the field of bioengineering and gene engineering technology. By means of molecular cloning technology, the luminous gene of Qinghai vibrio is introduced into Salmonella typhimurium TA97 to constitute engineering bacterium LZ-17. The engineering bacterium can produce bacterioleuciferinase, can glow with visible light of 440-640 nm wavelength after decanol is added, and has the Ames test relevant characters the same as Salmonella typhimurium TA97. The engineering bacterium is applied in Ames test as testee life form for determining the number of back varying bacterium. The engineering bacterium is especially suitable for detection of mutagenicity of the testee sample.

The invention relates to a test paper box which is based on a bacterial fluorescent staining technique and is used for detecting dyed salmonella typhimurium via immunochromatography by utilizing a single antibody. The test paper box comprises a single antibody test strip and a fluorochrome box. The test paper box has the advantages that the salmonella typhimurium is dyed in advance by utilizing fluorochrome and then is sprayed on a T line of the test strip by utilizing a specific antibody of the salmonella typhimurium so as to capture the salmonella typhimurium in a mixed sample, so that the salmonella typhimurium in the sample can be rapidly and specifically detected.

The invention provides a detection test strip of salmonella typhimurium. The detection test strip comprises a sample pad, a cellulose nitrate membrane, absorbent paper and a bottom plate. A quantum dot fluorescent micro-sphere is marked with a monoclonal antibody of salmonella typhimurium, and the cellulose nitrate membrane is coated with a detection antibody and a quality control antibody. Basedon the test strip, the invention also provides a method for high-sensitivity and quick quantitative detection of salmonella typhimurium. The method fully utilizes super-strong and stable fluorescent signals of quantum dots, combines the advantages of easy marking and control of polymer sub-micro-spheres, constructs an immunochromatography technology of quantum dot fluorescent micro-spheres, and realizes sensitive and quick detection of salmonella typhimurium.

The invention specifically relates to a method for detecting Salmonella typhimurium, belonging to the field of analytical chemistry. The method comprises the following steps: modifying LumAuNPs with probe DNA so as to obtain a chemiluminescence probe; modifying a magnetic bead by using captured DNA with a hairpin structure so as to obtain a captured DNA-modified magnetic bead; modifying another magnetic bead by using aptamer DNA and aptamer complementary sequence DNA so as to obtain a DNA-modified magnetic beads; adding a Salmonella typhimurium solution containing a target into a solution of the DNA-modified magnetic bead, carrying out magnetic separation, sucking up obtained clear liquid, adding the clear liquid into a solution of the captured DNA-modified magnetic bead, and connecting the chemiluminescence probe with the surface of the magnetic bead via catalytic hairpin self-assembly technology under the action of hybridization of the probe DNA on the chemiluminescence probe; and after magnetic separation and adding hydroxylamine-O-sulfonic acid for generation of chemiluminiscence so as to realize determination of Salmonella typhimurium. According to the invention, LumAuNPs-hydroxylamine-O-sulfonic acid is used as a chemiluminescence system, so the intensity of a detection signal is improved; and mismatched bases are introduced into the probe DNA, so background signals are reduced, and determination sensitivity is improved. The method provided by the invention has the advantages of easiness and high sensitivity.

The invention discloses a salmonella bacteriophage nano magnetic bead conjugate and its enrichment and separation kit. The conjugate is a complex of a salmonella bacteriophage coupled with a carboxylmagnetic bead, wherein the salmonella bacteriophage is Salmonella Typhimurium bacteriophage LPST10, and a preservation number is CCTCC NO: M 2016473. The bacteriophage of the Salmonella bacteriophagenano magnetic bead conjugate of the invention can specifically recognize and infect bacteria, and has the characteristics of good specificity and small molecular weight. The kit is capable of rapidlyand sensitively separating and enriching salmonella.

The invention discloses a staphylococcus aureus capsular polysaccharide and protein conjugate and preparation method and application thereof. Salmonella typhimurium flagellin or polylysine salmonella typhimurium flagellin is taken as a carrier, and staphylococcus aureus capsular polysaccharide is subject to chemical modification, and then chemical coupling is carried out on the staphylococcus aureus capsular polysaccharide and the carrier protein. As the salmonella typhimurium flagellin and polylysine salmonella typhimurium flagellin have immunologic adjuvant function, the new formed conjugate can cause staphylococcus aureus capsular polysaccharide with extremely low immunogenicity to have higher immunogenicity, and the carrier protein plays the immunologic adjuvant function when in inoculation, so that specific immune response of immune animal to the staphylococcus aureus capsular polysaccharide is enhanced. The invention has wide application in preparation of vaccine resisting staphylococcus aureus infection.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum and Salmonella pullorum, a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella enteritidis or Salmonella typhimurium, Fowl typhoid caused by Salmonella gallinarum, and Pullorum disease caused by Salmonella pullorum, which comprises the bacteriophage as an active ingredient, and an animal feed, drinking water, cleaner, and sanitizer which comprise the bacteriophage as an active ingredient.