108 results about "Salmonella ser. Typhimurium" patented technology

The present invention is directed to the isolation and use of super-infective, tumor-specific vectors that are strains of parasites including, but not limited to bacteria, fungi and protists. In certain embodiments the parasites include, but are not limited to, the bacterium Salmonella spp., such as Salmonella typhimurium, the bacterium Mycobacterium avium and the protozoan Leishmania amazonensis. In other embodiments, the present invention is concerned with the isolation of super-infective, tumor-specific, suicide gene-containing strains of parasites for use in treatment of solid tumors.

The invention discloses pseudomonas aeruginosa bacteriophage endolysins, and a coding gene and application thereof. The invention provides a protein which is as shown in (a) or (b) as below: (a) the protein which is as shown in a sequence 1 in a sequence table and consists of an amino acid sequence; (b) the protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence which is as shown in the sequence 1 in the sequence table, has a function of cracking gram-negative bacteria and is deviated from the sequence 1 in the sequencetable. The protein provided by the invention can crack a plurality of gram-negative bacteria, such as Escherichia coli, Klebsiella pneumonia, pseudomonas aeruginosa, Burkholderia cepacia and Salmonella typhimurium. The protein and a surfactant EDTA (Ethylene Diamine Tetraacetic Acid) act synergistically to achieve a stronger inhibiting effect on the gram-negative bacteria. A foundation is laid for researching and developing a new antibacterial preparation.

The present invention relates to a novel bacteriophage, and more specifically to a bacteriophage that can specifically destroy one or more Salmonella spp. selected from a group consisting of Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum and Salmonella pullorum. The present invention further relates to a composition containing the bacteriophage as an active ingredient for the prevention or treatment of infectious diseases such as salmonellosis and salmonella foodborne intoxication induced by Salmonella enteritidis or Salmonella typhimurium, fowl typhoid induced by Salmonella gallinarum, pullorum induced by Salmonella pullorum, etc. Moreover, the present invention relates to animal feeds, drinking water, detergent and disinfectant containing the bacteriophage as an inactive ingredient.

The invention provides a salmonella broad-spectrum virulent bacteriophage. The preservation number of the bacteriophage is CCTCC NO: M 2020839. The invention also provides a preparation process and application of the salmonella broad-spectrum virulent bacteriophage, and the preparation process comprises fermentation and extraction; in the step of fermentation, a salmonella gallinarum attenuated vaccine strain is used as host bacteria for bacteriophage fermentation. The bacteriophage XPARCPS02 disclosed by the invention can be used for cracking salmonella of various serotypes and also has different degrees of cracking effects on drug-resistant bacteria; the salmonella typhimurium lysate lysate has a cracking capability on salmonella typhimurium, salmonella typhimurium drug-resistant bacteria, salmonella gallinarum, salmonella enteritidis, salmonella enteritidis drug-resistant bacteria, salmonella typhimurium, salmonella pullorum, salmonella paratyphi A, salmonella paratyphi B and salmonella dubicularis.

The invention provides Salmonella typimurium S496. The Salmonella typimurium S496 is characterized by lacking an rfbB gene and an rffG gene; a pagL gene is replaced by an arabinose-regulatory-sequence-containing rfbB gene with a changed SD sequence and a changed initiation codon, and the nucleotide sequence of the arabinose-regulatory-sequence-containing rfbB gene with the changed SD sequence and the changed initiation codon is shown as SEQ ID No.1; the classification of the attenuation strain is named as the Salmonella typimurium S496, the Salmonella typimurium S496 is collected in the China Center For Type Culture Collection (CCTCC), the collection number is CCTCC M2015562, and the collection time is September 21st, 2015. The invention further provides a method for constructing the Salmonella typimurium S496. Meanwhile, the invention provides an application of the strain or the attenuation strain constructed with the construction method in vaccine.

The present invention provides the method for the production of the egg containing antipathogenic bacteria specific antibodies (IGY) preventing gastritis, diarrhoea, and food poisoning by immunizing young hens with antigen proteins of E. coli causing enteritis, Helicobacter pylori causing gastritis, and Salmonella enteritidis and Salmonella typhimurium causing food poisoning. Simultaneously this invention also relates to the composition containing the specific IGY antibodies described above and the foodstuff such as the yoghurt and ice cream containing the antipathogenic IGY. Additionally, the present invention provides the separation method of the IGY containing protein powders from egg yolk. Particularly, this separation method involves diluting egg yolk with water at 1:1 ratio and adding the appropriate amount of ammonium sulphatewhich enables water-soluble protein and phospholipid to separate.

In one aspect, methods and kits for determining the mutagenic potential of a test substance. The method includes exposing a tester strain (such as Salmonella typhimurium) to the substance, wherein the tester strain includes a gene (such as the histidine gene) having a preexisting mutation conferring auxotrophy, and the mutation is located at a pre-determined position in the gene, growing the tester strain in growth media lacking histidine, and detecting the presence of a back-mutation at the position by analysis of the nucleic acid. The tester strain can be selected from TA98, TA100, TA102 TA1535, TA1537, TA1538, and TA97. The test substance can be any of a wide variety of compounds such as petroleum extracts, pesticides, cosmetics, adhesives, herbicides, hair dyes, and pharmaceuticals. The detecting step can include one or more conventional mutation detection methods. Also provided are kits for conducting the method. The kits can include one or more tester strains, PCR primers, positive control compounds, and DNA polymerase.

The invention discloses a recombination attenuated salmonella typhimurium carrier vaccine of expression IBDV (Infectious Bursal Disease Virus) immunogenic gene, a preparation method and application thereof, in particular relates to oral attenuated salmonella typhimurium. The recombination attenuated salmonella typhimurium comprises a sequence disclosed by SEQ ID NO.1, and the recombination virus can express IBDV capsid protein VP2. The preparation method comprises the following steps of: inoculating the attenuated salmonella typhimurium in an LB substrate for culturing 18 hours; regulating bacterial concentration as 1010CFU / ml; and preparing the safe and effective oral attenuated salmonella typhimurium carrier vaccine for preventing IBD (Infectious Bursal Diseases).

The invention specifically relates to a method for detecting Salmonella typhimurium, belonging to the field of analytical chemistry. The method comprises the following steps: modifying LumAuNPs with probe DNA so as to obtain a chemiluminescence probe; modifying a magnetic bead by using captured DNA with a hairpin structure so as to obtain a captured DNA-modified magnetic bead; modifying another magnetic bead by using aptamer DNA and aptamer complementary sequence DNA so as to obtain a DNA-modified magnetic beads; adding a Salmonella typhimurium solution containing a target into a solution of the DNA-modified magnetic bead, carrying out magnetic separation, sucking up obtained clear liquid, adding the clear liquid into a solution of the captured DNA-modified magnetic bead, and connecting the chemiluminescence probe with the surface of the magnetic bead via catalytic hairpin self-assembly technology under the action of hybridization of the probe DNA on the chemiluminescence probe; and after magnetic separation and adding hydroxylamine-O-sulfonic acid for generation of chemiluminiscence so as to realize determination of Salmonella typhimurium. According to the invention, LumAuNPs-hydroxylamine-O-sulfonic acid is used as a chemiluminescence system, so the intensity of a detection signal is improved; and mismatched bases are introduced into the probe DNA, so background signals are reduced, and determination sensitivity is improved. The method provided by the invention has the advantages of easiness and high sensitivity.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum and Salmonella pullorum, a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella enteritidis or Salmonella typhimurium, Fowl typhoid caused by Salmonella gallinarum, and Pullorum disease caused by Salmonella pullorum, which comprises the bacteriophage as an active ingredient, and an animal feed, drinking water, cleaner, and sanitizer which comprise the bacteriophage as an active ingredient.

The invention discloses an oral recombinant attenuated salmonella typhimurium live vector vaccine as well as a preparation method and application thereof, particularly relating to an oral attenuated salmonella typhimurium. The recombinant attenuated salmonella typhimurium has a sequence shown in SEQ ID NO.1, and can express PCV-2 capsid protein ORF2. The attenuated salmonella typhimurium is inoculated in an LB culture medium for culturing for 18 hours; and the concentration of the attenuated salmonella typhimurium is regulated to 1010CFU / ml to prepare a safe and effective oral attenuated salmonella typhimurium live vector vaccine used for preventing porcine circovirus diseases.