Method for production of egg containing anti-pathogenic bacteria specific antibodies (IgY) and yogurt and ice cream containing IgY

A production method and specific protein technology, which is applied to the preparation method of peptides, chemical instruments and methods, anti-bacterial immunoglobulin, etc., can solve the problems of high cost, low efficiency of purification and separation of a large number of products, and achieve cost reduction and efficiency. Effect that there is little loss of price

Inactive Publication Date: 2003-04-16
EGG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The prior art described above, although used to isolate water-soluble proteins and lipids from egg yolk, is limited by inefficiencies and high costs for purifying and isolating large quantities of products on an industrial scale

Method used

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  • Method for production of egg containing anti-pathogenic bacteria specific antibodies (IgY) and yogurt and ice cream containing IgY
  • Method for production of egg containing anti-pathogenic bacteria specific antibodies (IgY) and yogurt and ice cream containing IgY
  • Method for production of egg containing anti-pathogenic bacteria specific antibodies (IgY) and yogurt and ice cream containing IgY

Examples

Experimental program
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Effect test

Embodiment 2

[0090] Example 2 relates to a mixture of specific immune proteins produced by mixing individual antibodies prepared separately, and to yogurt and ice cream containing specific immune proteins extracted from eggs produced in the same way. 1. Isolation and Characterization of Enteropathogenic Escherichia coli and Antigen Production

[0091] The enteropathogenic Escherichia coli used in the present invention was isolated from humans. Isolation and identification of enteropathogenic Escherichia coli (ETEC) and production of antibodies were performed as described in Example 1. 2. Production of Helicobacter pylori Antigens

[0092] Production of H. pylori antigens was performed as described in Example 1. 3. Production of Salmonella enteritidis antigen and Salmonella typhimurium antigen.

[0093] Production of S. Enteritidis antigen and S. Typhimurium antigen was performed as described in Example 1. 4. Inject the chicks with 4 kinds of antigens and give the laying hens a booster ...

Embodiment 4

[0107] In order to remove fat, water-soluble protein and pigment in the egg yolk remaining in the fat-separated solution, the following experiment was performed.

[0108] Seven treatments were diluted with distilled water by factors of ×6, ×12, ×18, ×30, ×42, ×48 and ×60 and incubated at 5°C for one day. Carefully separate the supernatant free of the pellet. The ×6 diluted treatment contained a precipitate, the yellow color of the egg yolk remained in the supernatant, and the precipitate was mixed with the supernatant after a while. In the ×12, ×18 and ×30 diluted treatments, the precipitate was not easily mixed with the supernatant. However, the diluted treatments of ×42, ×48 and ×60 contained precipitates, but were easily mixed with the supernatant. Therefore, the appropriate dilution factors for the precipitate are ×12 and ×18, and the precipitate is so viscous that it needs to be washed away with tap water. At the same time, the IgY titer of the supernatant was measured, ...

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Abstract

The present invention provides the method for the production of the egg containing antipathogenic bacteria specific antibodies (IGY) preventing gastritis, diarrhoea, and food poisoning by immunizing young hens with antigen proteins of E. coli causing enteritis, Helicobacter pylori causing gastritis, and Salmonella enteritidis and Salmonella typhimurium causing food poisoning. Simultaneously this invention also relates to the composition containing the specific IGY antibodies described above and the foodstuff such as the yoghurt and ice cream containing the antipathogenic IGY. Additionally, the present invention provides the separation method of the IGY containing protein powders from egg yolk. Particularly, this separation method involves diluting egg yolk with water at 1:1 ratio and adding the appropriate amount of ammonium sulphatewhich enables water-soluble protein and phospholipid to separate.

Description

technical field [0001] The present invention provides eggs that contain specific antibodies (IgY) against pathogenic bacteria to prevent gastritis by immunizing chickens with antigenic proteins of E. , diarrhea and food poisoning methods, also provides a composition of protein powder mixed with specific antibodies as described above in an appropriate ratio, the composition is produced by immunization with four antigens alone, and food processed with milk, such as Yogurt and ice cream, which contain specific antibodies against pathogenic bacteria (IgY). [0002] In addition, for the method of separating specific antibody protein powder, the method of separating protein and phospholipids is especially diluting egg yolk with distilled water at a ratio of 1:1, adding an appropriate amount of ammonium sulfate, which separates water-soluble protein from phospholipids, and separates egg yolk from water-soluble The protein method dilutes the separation solution with distilled water a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/00A01K67/027A23C9/13A23C9/152A23G9/32A23G9/38A23G9/44A23G9/52A23L1/305A23L15/00A23L27/60A61K39/395A61P1/04C07K1/30C07K1/34C07K16/12
CPCC07K16/1235A23L1/3212C07K16/121C07K2317/11C07K16/1232A23G9/38A23C9/1315C07K2317/23A23L1/3204A23L15/20A23L15/30A61P1/04
Inventor 李南珩柳贞秀郑光龙白磻石鲜于宣泳
Owner EGG BIOTECH
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