58 results about "Salmonella frintrop" patented technology

The invention belongs to the technical field of microbiological detection, and discloses a composite probe for detecting salmonella enteritidis and a preparation method. The composite probe for detecting the salmonella enteritidis comprises magnetic nanoparticles FeO, wherein the surface of the magnetic nanoparticles FeO is modified with an aptamer apt which is specifically combined with the salmonella enteritidis; the surface of the long afterglow nano particle PLNPs is modified with a DNA (Deoxyribose Nucleic Acid) sequence cDNA (Complementary Deoxyribonucleic Acid) complementary with the aptamer; the aptamer apt and the complementary DNA sequence cDNA are subjected to hybridization to form a composite probe structure FeO-SEapt (at) PLNPs-cDNA (complementary deoxyribonucleic acid). The long afterglow luminescence characteristic of PLNPs (ZnGaO: Cr) is utilized, and an excitation light source is stopped before detection, so that signal acquisition completely avoids autofluorescence of a sample matrix, background fluorescence interference is thoroughly eliminated, and the signal-to-noise ratio is remarkably improved.

The invention relates to the technical field of genetic engineering, in particular to a long-chain RNALnc012227 for inhibiting transmission of salmonella enteritidis ovary and application of the long-chain RNALnc012227 in prevention and control of salmonella enteritidis infection. The invention relates to a long-chain RNA (Ribonucleic Acid) Lnc012227 for inhibiting transmission of salmonella enteritidis ovary. The Lnc012227 provided by the invention can be used for indirectly regulating the expression of MAP3K8, activating NF-kappa B and MAPK signal channels and enhancing the inflammatory response of a host, so that S.Enteridis infection is controlled. The discovery not only provides a new understanding of molecular level for understanding the infection mechanism of salmonella in the duck body, but also provides a potential target spot for developing a new treatment strategy, and is helpful for reducing egg-laying duck ovarian infection and duck egg pollution caused by salmonella.

This invention belongs to the field of molecular biology detection technology, specifically relating to a primer and probe combination for detecting avian Salmonella based on the RAA-LFD method and its application, particularly to a primer and probe combination for detecting three avian serotypes of Salmonella: Salmonella pullorum, Salmonella enteritidis, and Salmonella typhimurium, based on the RAA-LFD method and its application. This invention designs different types of probes and primers targeting the genes of serotypes SP, SE, and STM of Salmonella. Using the primer and probe combination of this invention, rapid, sensitive, and specific detection of Salmonella serotypes and the three avian Salmonella serotypes SP, SE, and STM can be achieved, greatly shortening the detection cycle of avian salmonellosis and improving the detection efficiency and accuracy of avian salmonellosis.

The present invention relates to the modification of an attenuated live strain of Salmonella enterica serovar Typhi, wherein its native surface-exposed polysaccharide and flagellin antigens can be converted to, or enhanced by, those of other strains of Salmonella, including Salmonella enterica serovar Paratyphi, Salmonella enterica serovar Typhimurium, and Salmonella enterica serovar Enteritidis. The present invention also relates to a modified strain of Salmonella enterica serovar Typhi suitable for use as a component of a vaccine against typhoid enteric fever and salmonellosis.

The present invention provides a salivarius lactobacillus 8-2 strain and an application thereof, wherein the salivarius lactobacillus 8-2 strain has been deposited in the General Microbiology Center of the China Microbiological Culture Collection Administration Committee, and the deposit number is CGMCC NO.30612. The salivarius lactobacillus described in the present invention has good acid resistance, is non-hemolytic, has strong antibacterial ability, can reduce the damage caused by salmonella infection, and can effectively promote the absorption function of intestinal mucosa, improve the production performance of laying hens, has the potential to be developed into a high-quality probiotic preparation for laying hens with both "antibiotic replacement" function and the function of promoting the repair of intestinal mucosal damage, and can be used in the preparation of laying hen antibiotic substitutes, including the application of the fermentation supernatant of the strain in the preparation of laying hen antibiotic substitutes.

The present invention relates to compounds of formula (Ia) which block the invasion-related pathogenicity of Salmonella typhimurium by inhibiting the activity of the transcription factor HilD. These compounds are useful in the treatment or prevention of Salmonella infection (salmonellosis).

The invention discloses a method for establishing a salmonella pullorum challenge model, and relates to the technical field of biological medicines. The invention discloses a method for establishing a salmonella pullorum challenge model. The method comprises the following steps: taking a 12-day-old chick embryo, and injecting a chitosan oligosaccharide solution into a chick embryo air chamber; placing the chick embryos injected with the chitosan oligosaccharide solution into an incubator for incubation until the chick embryos are 14 days old; salmonella pullorum is injected into an air chamber of the 14-day-old chick embryo for challenging; after challenging the chick embryos, continuously incubating the chick embryos in an incubator until 21 days old; and after the chick embryos are incubated to 21 days old, hatched and changed into chicks, establishing a toxic attack model. According to the invention, the method of the challenge model is creatively optimized, and the optimal chitosan solution injection concentration and the optimal challenge concentration of salmonella pullorum are screened; the salmonella pullorum challenge model is built through the method, the highest morbidity and survival rate of chicks can be controlled after 24 h, and it can be guaranteed that the morbidity time is uniform.

Every year, approximately 94 million cases of Salmonella gastroenteritis, with 155000 deaths, are reported each year and 85% of them reported to be food-borne. Investigation of the foods whether they are clean for Salmonella and sensitivity, easy applicability, absence of false positivity and negativity and the speed are the features sought in the analysis method for this investigation. It is not desirable for analysis to detect the presence of dead bacteria in food. Although the final product does not contain microbiologically harmful live bacteria during the food process, the detection of dead bacteria transmitted before the process causes the food product to be unfairly diagnosed as harmful. To prevent this situation, the analysis kits depending on molecular methods, increase their microorganism detection levels up to to 104 while reducing their sensitivity. Since the molecular methods cannot discriminate dead and live organisms, a confirmation test is required to prove that the positive result of the analysis belongs to the live bacteria in the food, which results in additional cost and time loss. In the same way, it is necessary to verify whether the colonies that grow in the gold standard culture method, belong to Salmonella bacteria. In the developed system; 105 dead bacterial DNA is eliminated in the food to prevent false positive results and the minimum detection limit is 10 bacteria. Also, in developed system, 4 primers specific to 6 regions of DNA are used. Therefore, the specificity of the method is very high (99.9%) and no verification test is needed. Since PCR systems require a device with complex temperature control units, they can make analysis in a laboratory-dependent manner. In the proposed system, DNA is amplified at constant temperature; no temperature cycle is required, therefore no complex instrument and laboratory infrastructure are required. All the procedures can be easily performed outside the laboratory on a portable mini-heater where pre-enrichment, DNA isolation from the sample and PCR steps are performed. For molecular analyses, the device is required to display the result of imaging or analysis. In the developed method, DNAs amplified by the loop-mediated isothermal DNA amplification method, are hybridized and combined with the labeled probe and then can be read by lateral flow method with the naked eye. As the results are visible by eye, no additional device is required. The classical culture method is accepted as the gold standard, but the duration of analysis is 7 days for positive samples, 3 days with verification test, for the molecular methods, and 5.5 hours including pre-enrichment time in the developed system.

The present application relates to the technical field of biological pharmacy, in particular to a combined drug for treating colitis and application thereof, wherein the volume ratio of Coptis exosome and Salmonella outer membrane vesicle in the combined drug is 1-2:1-3; the concentration of the Coptis exosome and the Salmonella outer membrane vesicle is 9-11 mg / mL. The present application overcomes the limitation of single treatment strategy by innovatively combining the Coptis exosome and the Salmonella outer membrane vesicle, significantly improves the treatment effect, and avoids the side effects of existing drugs. The present application has lower treatment cost, is safer and has no drug resistance problem, and can provide a more effective, safe and economical treatment method for inflammatory diseases such as colitis.

The invention discloses a primer group for simultaneously detecting four equine digestive tract bacteria and application thereof, the primer group comprises primer sequences as shown in SEQ ID NO: 1-8, and the four equine digestive tract bacteria are salmonella enteritidis, salmonella typhimurium, clostridium difficile and lawsonia intracellular. Extracting total DNA (deoxyribonucleic acid) of a sample to be detected by using the excrement sample nucleic acid extraction kit; the total DNA is used as a template, and the primer group is used for multiple PCR reaction to obtain an amplification curve. According to the invention, a primer sequence with high sensitivity and specificity is adopted, so that the quality of a detection result is ensured; the detection method is simple to operate, time-saving and labor-saving; the detection flux is high, and the reagent consumable cost is low.

The invention discloses a cdLAMP-based detection kit for simultaneously detecting staphylococcus aureus and salmonella and a detection method thereof, and relates to the field of biological detection.The detection kit comprises a silicon-based nano porous chip, a primer probe combination, 20 mM Tris-HCl, 10 mM KCl, 8 mM MgSO4, 10 mM (NH4) 2SO4, 0.05% Tween-20, a 2.5 mu L DNA template, 1.4 mM dNTP, 0.16 U / mu L Bst DNA polymerase and DEPC; the primer probe combination comprises a primer probe and a primer probe, wherein the primer probe is designed according to a nuc gene of staphylococcus aureus: GenBank registration number EF529607.1, and the primer probe is designed according to a fimY gene of salmonella: GenBank registration number JQ665438.1; according to the invention, dual detection is realized through a chip and a two-color fluorescence channel carried by an imaging analyzer, and compared with the existing detection method, the method has higher sensitivity and accuracy.

In one aspect, the disclosure relates to compositions and methods for dispersing exiting Salmonella biofilms and inhibiting formation of Salmonella biofilms. In various aspects, the disclosed compositions can be used in methods of treating a persistent Salmonella infection, including an asymptomatic infection. Such infections can colonize a variety of tissues, including the gall-bladder. Also disclosed are methods of treating typhoid fever. Also disclosed are methods for mitigating or preventing secondary outbreaks of typhoid fever by treating asymptomatic subjects who had been symptomatic for typhoid fever at a previous time. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

The present invention relates to a bacteriocin (salmonin) for controlling Salmonella enterica. The bacteriocin is derived from Salmonella enterica. The salmonin can be expressed in plants and can be used in a method for preventing or reducing Salmonella enterica infection or contamination of a target.

An aptamer biosensor for detecting Salmonella, its preparation method and application belong to the technical field of food safety. In order to solve the problems existing in the detection of Salmonella in the prior art, the present invention provides an aptamer biosensor. Its detection principle is that the sandwich structure SMBs-Apt2-AuNPs fluorescence quenching system established by the SMBs-cDNA2 complex, aptamer Apt2 and AuNPs-cDNA3 probe can maintain integrity in the absence of Salmonella, and the AuNPs in the system undergo FRET with AgNCs to quench the fluorescence of AgNCs; in the presence of Salmonella, Salmonella binds to Apt2 as a connecting bridge, and the SMBs-Apt2-AuNPs system is destroyed, and the AuNPs-cDNA3 probe suspended in the supernatant and Salmonella binding Apt2 are removed by magnetic separation, resulting in the fluorescence not being quenched. The aptamer biosensor obtained by the present invention has the characteristics of simplicity, low cost, fast speed and high sensitivity, and can be used for real-time detection of Salmonella contamination in food and food processing, especially Salmonella contamination in dairy product production.

Provided herein are methods and compositions for detecting Salmonella Typhimurium in a sample.

The invention provides application of piceatannol in preparation of a salmonella flagellum inhibitor, and belongs to the technical field of biological medicines. Through analysis of salmonella sliding motion test, biofilm formation test, salmonella cell invasion test and other tests, it is found that piceatannol down-regulates flagellum-related gene transcription, weakens the sliding capacity, reduces biofilm formation and weakens the invasion capacity to host cells at non-inhibitory concentration; the invention relates to a salmonella flagellum inhibitor, particularly relates to a salmonella flagellum inhibitor, supports the development value of the salmonella flagellum inhibitor as an anti-virulence drug, provides a non-bactericidal treatment strategy capable of remarkably weakening bacterial pathogenicity, can be used as a potential lead compound or a composition therapy component for treating salmonella infection, and is applied to production and preparation of the salmonella flagellum inhibitor.

The present disclosure relates to a recombinant expression vector for secretion of melittin and an attenuated Salmonella strain transformed therewith, and provides an attenuated Salmonella strain transformed with a recombinant expression vector including an flgM gene and a melittin gene, an anticancer pharmaceutical composition including the attenuated Salmonella strain as an active ingredient, and a recombinant expression vector capable of improving the secretion ability of melittin.

The application discloses a nanobody for detecting salmonella typhimurium, and aims to provide a nanobody for detecting salmonella typhimurium, which has high detection accuracy, high specificity and simple obtaining method. The amino acid sequence of the nanobody is shown in SEQ-Styp NO. 1, SEQ-Styp NO. 2, SEQ-Styp NO. 3 and SEQ-Styp NO. 4, and the nanobody belongs to the technical field of biological detection.

The composition and methods of treatment disclosed or contemplated herein include compositions and methods of treatment for liver-related diseases. The overall treatment strategy would involve the identification of a target protein or receptor within the liver that is responsible for ongoing liver injury. The strain of orally administered Salmonella Typhimurium that colonizes the liver would be engineered to secrete a particular protein or nanobody that would stop and / or reverse the progressive hepatic disease

The invention relates to the technical field of microorganisms, in particular to streptococcus gallate and application thereof in preparation of a salmonella infection resistant preparation. The streptococcus gallate can be used for improving the resistance of a host to salmonella infection, and can be used for preparing a probiotic preparation, a functional feed additive or a related health care product for preventing or assisting in treating the salmonella infection.

The present invention relates to a DNA construct comprising: a gene encoding flagellin and a gene encoding an immunopotentiator (or adjuvant) protein. In order to achieve effective cancer treatment by selectively killing only cancer cells, the attenuated Salmonella strains of the present disclosure are designed to produce immunogenic substances in cancer tissue to induce a strong anti-cancer immune response to significantly inhibit tumor size in primary and metastatic cancers. Therefore, the strain can be advantageously used in a prophylactic or therapeutic composition for increasing survival rate.

Provided herein are vaccine compositions comprising a Salmonella antigen conjugated to a capsid wherein the capsid comprises a wild-type or native sequence. Also provided herein are vaccine compositions comprising a Salmonella antigen conjugated to a capsid wherein the capsid comprises at least one mutation, such as a non-natural mutation. Such compositions are useful in the prevention or treatment of Salmonella infection (salmonellosis), in the treatment and prevention of gastroenteritis, typhoid and / or paratyphoid; and the method can effectively aim at various salmonella strains.

The application belongs to the technical field of biological medicine, and discloses a recombinant protein subunit vaccine constructed by combining Salmonella flagellin and a COE region of porcine epidemic diarrhea virus (PEDV). The application specifically discloses a fusion protein, wherein the fusion protein comprises Salmonella flagellin FliC L3A a mutant and a PEDV structural protein connected to the C terminal of the Salmonella flagellin FliC L3A The subunit vaccine obtained by emulsifying the fusion protein and the adjuvant can effectively prevent the G2b subfamily of PEDV, initiate a downstream immune response, induce a series of immune responses of the body, make the mucosal immune system of the body produce a large number of PEDV-specific antibodies to resist the invasion of external pathogens, and has the effect of specific immune protection.

Provided are a method for preparing freezing shock-treated monocytes or macrophages loaded with attenuated Salmonella and the use thereof. The method comprises: loading engineering-modified attenuated Salmonella in monocytes or macrophages, and performed a freezing shock treatment on the monocytes or macrophages loaded with the strain by means of liquid nitrogen. The method for preparing the freezing shock-treated immune cells loaded with the strain is simple and easy to operate. The source problem of the monocytes or macrophages loaded with the engineering-modified attenuated Salmonella is solved, and the method has good application prospects. According to the strategy, by means of avoiding exposure and heterologous stimulation of bacteria, the biological safety of bacteria-based anti-tumor treatment is improved; and the anti-tumor immune response is promoted by means of the high titer of an intratumoral strain, so that a stronger anti-tumor efficacy is obtained.

The present disclosure relates to a recombinant expression vector for secretion of interleukin-21 and an attenuated Salmonella strain transformed therewith, and provides: a recombinant expression vector comprising an flgM gene and an interleukin-21 gene; an attenuated Salmonella strain transformed therewith; and a pharmaceutical composition for cancer treatment, comprising the attenuated Salmonella strain.

The composition and methods of treatment disclosed or contemplated herein include compositions and methods of treatment for liver-related diseases. The overall treatment strategy would involve the identification of a target protein or receptor within the liver that is responsible for ongoing liver injury. The strain of orally administered Salmonella Typhimurium that colonizes the liver would be engineered to secrete a particular protein or nanobody that would stop and / or reverse the progressive hepatic disease

The composition and methods of treatment disclosed or contemplated herein include compositions and methods of treatment for liver-related diseases. The overall treatment strategy would involve the identification of a target protein or receptor within the liver that is responsible for ongoing liver injury. The strain of orally administered Salmonella Typhimurium that colonizes the liver would be engineered to secrete a particular protein or nanobody that would stop and / or reverse the progressive hepatic disease

The present invention relates to the technical field of probiotics, in particular to a Lactiplantibacillus plantarum GOLDGUT-LP618 having a function of resisting Salmonella infection and application thereof. The Lactiplantibacillus plantarum GOLDGUT-LP618 provided by the present invention is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 28399. The strain has high resistance to acid and bile salt and can adapt to the digestive tract environment. The strain can inhibit Salmonella infection, relieve diarrhea, inflammation and intestinal damage caused by Salmonella infection, and promote intestinal health. The strain can be used for preparing functional foods, dietary supplements or drugs that can resist Salmonella infection and improve diarrhea and inflammation caused by Salmonella infection, and has good application prospects.

The invention discloses a salmonella detection primer combination and a detection method, and relates to the technical field of bioengineering. The primer is fimY-RPA-F3, and the sequence of the primer is as shown in SEQ ID No: 7; and the sequence of the fimY-RPA-R3 is as shown in SEQ ID No: 8. In order to establish a salmonella RPA rapid detection method, by optimizing primer design, reaction conditions and a detection process, the detection sensitivity and specificity are improved, the detection time is shortened, and the operation process is simplified, so that the requirement of on-site rapid detection is met, and a technical support is provided for food safety supervision and public health prevention and control.