16 results about "Salmonella frintrop" patented technology

This invention belongs to the field of molecular biology detection technology, specifically relating to a primer and probe combination for detecting avian Salmonella based on the RAA-LFD method and its application, particularly to a primer and probe combination for detecting three avian serotypes of Salmonella: Salmonella pullorum, Salmonella enteritidis, and Salmonella typhimurium, based on the RAA-LFD method and its application. This invention designs different types of probes and primers targeting the genes of serotypes SP, SE, and STM of Salmonella. Using the primer and probe combination of this invention, rapid, sensitive, and specific detection of Salmonella serotypes and the three avian Salmonella serotypes SP, SE, and STM can be achieved, greatly shortening the detection cycle of avian salmonellosis and improving the detection efficiency and accuracy of avian salmonellosis.

The present application relates to the technical field of biological pharmacy, in particular to a combined drug for treating colitis and application thereof, wherein the volume ratio of Coptis exosome and Salmonella outer membrane vesicle in the combined drug is 1-2:1-3; the concentration of the Coptis exosome and the Salmonella outer membrane vesicle is 9-11 mg / mL. The present application overcomes the limitation of single treatment strategy by innovatively combining the Coptis exosome and the Salmonella outer membrane vesicle, significantly improves the treatment effect, and avoids the side effects of existing drugs. The present application has lower treatment cost, is safer and has no drug resistance problem, and can provide a more effective, safe and economical treatment method for inflammatory diseases such as colitis.

The invention discloses a primer group for simultaneously detecting four equine digestive tract bacteria and application thereof, the primer group comprises primer sequences as shown in SEQ ID NO: 1-8, and the four equine digestive tract bacteria are salmonella enteritidis, salmonella typhimurium, clostridium difficile and lawsonia intracellular. Extracting total DNA (deoxyribonucleic acid) of a sample to be detected by using the excrement sample nucleic acid extraction kit; the total DNA is used as a template, and the primer group is used for multiple PCR reaction to obtain an amplification curve. According to the invention, a primer sequence with high sensitivity and specificity is adopted, so that the quality of a detection result is ensured; the detection method is simple to operate, time-saving and labor-saving; the detection flux is high, and the reagent consumable cost is low.

The invention provides application of piceatannol in preparation of a salmonella flagellum inhibitor, and belongs to the technical field of biological medicines. Through analysis of salmonella sliding motion test, biofilm formation test, salmonella cell invasion test and other tests, it is found that piceatannol down-regulates flagellum-related gene transcription, weakens the sliding capacity, reduces biofilm formation and weakens the invasion capacity to host cells at non-inhibitory concentration; the invention relates to a salmonella flagellum inhibitor, particularly relates to a salmonella flagellum inhibitor, supports the development value of the salmonella flagellum inhibitor as an anti-virulence drug, provides a non-bactericidal treatment strategy capable of remarkably weakening bacterial pathogenicity, can be used as a potential lead compound or a composition therapy component for treating salmonella infection, and is applied to production and preparation of the salmonella flagellum inhibitor.

The invention relates to the technical field of microorganisms, in particular to streptococcus gallate and application thereof in preparation of a salmonella infection resistant preparation. The streptococcus gallate can be used for improving the resistance of a host to salmonella infection, and can be used for preparing a probiotic preparation, a functional feed additive or a related health care product for preventing or assisting in treating the salmonella infection.

The composition and methods of treatment disclosed or contemplated herein include compositions and methods of treatment for liver-related diseases. The overall treatment strategy would involve the identification of a target protein or receptor within the liver that is responsible for ongoing liver injury. The strain of orally administered Salmonella Typhimurium that colonizes the liver would be engineered to secrete a particular protein or nanobody that would stop and / or reverse the progressive hepatic disease

The composition and methods of treatment disclosed or contemplated herein include compositions and methods of treatment for liver-related diseases. The overall treatment strategy would involve the identification of a target protein or receptor within the liver that is responsible for ongoing liver injury. The strain of orally administered Salmonella Typhimurium that colonizes the liver would be engineered to secrete a particular protein or nanobody that would stop and / or reverse the progressive hepatic disease

The present utility model provides a novel diagnostic tool for the detection of Salmonella enterica serovar Typhimurium. Specifically, the utility model relates to a developed and optimized simple and fast loop-mediated isothermal amplification (LAMP)-based assay parallel with optimized conventional PCR assay to detect Salmonella Typhimurium targeting the STM4497 gene. A critical feature of the utility model is the utilization of closed-tube protocols calcein-based LAMP detection assay for visualization in post-enrichment steps to prevent cross-contamination events. Another critical feature is the incorporation of optimized betaine concentration to prevent non-specific amplifications. The method is specifically designed for a simple, sensitive, specific and fast surveillance method for S. Typhimurium that lessens the turnaround time and shorter amplification time than conventional methods.

Provided herein is the ability for intravenous delivery of bacteria without the septic side-effects and for delivery of immune modulating proteins that are systemically toxic to be delivered directly to the tumor micro-environment without any systemic toxicity.

The application discloses a composite antibacterial pet external spray containing plant extracts and a preparation method thereof, and belongs to the technical field of pet care products. The external spray contains, in percentage by mass, 0.5-1.0% of cortex dictamni root extract, 0.75-1.5% of lycopersicum fruit extract, 0.75-1.5% of artemisia argyi leaf extract and 5-8% of chrysanthemum parthenium pure distillate, and can be further combined with auxiliary components such as emulsifiers and preservatives. The cortex dictamni root extract is prepared by using a supercritical CO2 extraction method combined with a specific composite entrainer, and active components are fully reserved; the spray is prepared by using a water phase and an oil phase in steps and is formed by high-shear emulsification, and has good stability. The product shows high bacteriostatic effects on staphylococcus aureus, salmonella and candida albicans, can significantly reduce the contents of inflammatory mediators NO and proinflammatory factors IL-1beta, has the effects of bacteriostasis, sterilization and anti-inflammation, is suitable for the preparation of pet skin care related products, and has a wide application prospect.

The application discloses a kind of attenuated salmonella and amodiaquine combined medicine and application, attenuated salmonella and amodiaquine combined medicine include attenuated salmonella typhimurium VNP20009 and its derivative or genetically modified strain and amodiaquine as active component.Amodiaquine is selectively eliminated neutrophil in tumor microenvironment by targeting binding glutathione reductase in neutrophil and inhibiting its activity.Amodiaquine of the application directly targets glutathione reductase in neutrophil, inhibits its activity, reduces reduced glutathione level, increases active oxygen accumulation, selectively induces neutrophil apoptosis in tumor immune microenvironment, and further enhances the colonization of VNP20009 and its derivative or genetically modified strain in tumor.The combination therapy reduces tumor weight by about 50%, prolongs tumor growth doubling time by 1.5 times, and does not affect the neutrophil level in peripheral blood and important organs, significantly reduces the liver and kidney toxicity of VNP20009 and its derivative or genetically modified strain.

This invention discloses a primer, kit, and application for rapid detection of Salmonella. The detection kit consists of a rapid nucleic acid amplification reagent, a fluorescent immunochromatographic test strip, and a running buffer. The rapid nucleic acid amplification reagent includes a graphene-containing amplification buffer, Salmonella-specific amplification primers, and quality control materials. The fluorescent immunochromatographic test strip consists of a sample pad, a binding pad coated with a fluorescent microsphere antibody (a marker at one end of the DNA amplification product) conjugate, an NC membrane containing a detection line (a marker at the other end of the DNA amplification product) and a control line, absorbent paper, and a PVC adhesive plate. After the rapid nucleic acid amplification reaction is completed, the running buffer is added, mixed, and then dropped onto the sample application window of the test strip. After 2 minutes, the fluorescence signal is read using a fluorescence analyzer. The entire process can be completed within 30 minutes. This Salmonella rapid detection kit is simple, rapid, sensitive, and provides reliable results, making it beneficial for practical applications.

The present application relates to a strain of Bacteroides fragilis and its application, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 14, 2026, and the preservation number is: CGMCC No. 38257.The strain can regulate STING-dependent training immunity, effectively resist Salmonella typhimurium and H9N2 avian influenza virus infection, maintain the integrity of the intestinal barrier of the host, and reduce inflammatory damage.The Bacteroides fragilis of the present application has good biological safety, and the strain can be used as a training immunity inducer, and the strain is expected to be a candidate probiotic or vaccine adjuvant for preventing / treating bacterial and viral infections, suitable for human health and poultry farming, and provides a new solution to resist antibiotic resistance.

The invention belongs to the technical field of biology, and discloses application, a screening method and a medicine of Brawler's bacteria, and application of the Brawler's bacteria to preparation of anti-inflammatory drugs. Firstly, it is determined that intestinal microorganisms play an important role in resisting salmonella infection through a fecal microorganism transplantation test; secondly, screening is carried out on excrement extracts of chicken infected with salmonella, it is found that the abundance of the Brawler is extremely high, and it is further found through in-vitro tests and in-vivo tests that the Brawler can inhibit the systemic inflammatory response, caused by infection of salmonella enteritidis, of the chicken.