74 results about "Salmonella frintrop" patented technology

The invention provides a visual detection method for mouse typhus salmonella based on aptamer recognition. The basic principle of the method is that the surface of a microwell plate is coated with avidin, sulfhydrylation salmonella aptamer which is marked by biotinylation salmonella aptamer and nanogold is adopted to recognize targeted salmonella, and on the basis, a silver enhancement technique is utilized to further amplify a detection signal, so that the visual detection is achieved. The visual detection method for the mouse typhus salmonella based on the aptamer recognition has the advantages of high specificity, strong sensitivity, simplification and rapidness, thereby providing the possibility for rapidly detecting the mouse typhus salmonella in an environment.

The invention relates to an aptamer based salmonella typhimurium detection biosensor and a preparation method thereof. Signal amplification is realized by continuous generation and cyclic utilization of Primer, and consequently high-sensitivity detection of salmonella typhimurium is realized, and lower detection limit can be acquired. The aptamer based salmonella typhimurium detection biosensor is high in specificity and sensitivity, low in cost and quick in detection. By the detection method, a salmonella typhimurium detection limit is 5-15cfu/mL, and a detection range is 1*10-1*107cfu/mL.

The present invention relates to Salmonella enterica comprising at least one pgl operon of Campylobacter jejuni or a functional derivative thereof and presenting at least one N-glycan of Campylobacter jejuni or N-glycan derivative thereof on its cell surface. In addition, it is directed to medical uses and pharmaceutical compositions thereof as well as methods for treating and/or preventing Campylobacter and optionally Salmonella infections and methods for producing these Salmonella strains.

The present invention relates to a method for rapidly detecting quinolone-resistant Salmonella spp. The invention also relates to the oligonucleotide primers and probes utilized in the method, which can differentiate mutant strains having one or two single point mutations in gyrA gene or single point mutation in parC gene from wild type strains, respectively. Said primers and probes can be effectively used for detection of nalidixic acid-resistant and/or ciprofloxacin-resistant Salmonella strains.

The invention belongs to the technical field of food quality safety detection, and particularly relates to a method in which the processes of fast extraction of co-enrichment and bacterium gene groups and multiple Taqman fluorescence quantitative PCR are used to carry out simultaneous and fast detection on salmonella, staphylococcus aureus and shigella in food and a recombined positive internal reference DNA is designed to monitor PCR reaction so as to guarantee the accuracy of a detected result. The result proves that the method has good specificity for target pathogenic bacteria, the detection limit can reach 101cfu/PCR, and all components of a reaction system do not generate cross interference. Blind sample detection on artificially contaminated foods verifies the practicability of the method. Positive internal reference DNA effectively guarantees the reliability of detection, and avoids the occurrence of false negative results. In conclusion, the invention establishes a multiple fluorescence quantitative PCR method for simultaneous and fast detecting staphylococcus aureus, salmonella and shigella in the food, and provides a new method for high-throughput detection of food origin diseases.

The invention relates to a fowl salmonella gallinarum attenuated isolated strain and application thereof. The isolated strain is preserved in the China General Microbiological Culture Collection Center, Beijing, China, on July 2, 2018, has a preservation number of CGMCC No.16049, and is classified and named as Salmonella Gallinarum. The strain is attenuated to chicken of different ages, is apathogenic, and can be used as a strain vector for preventing livestock salmonella gallinarum infection and preventing infectious diseases of livestock (chicken). The fowl salmonella gallinarum attenuated isolated strain provides data reference to evaluating the toxicity of salmonella attenuated vaccine strains and potential as the strain vector of livestock (chicken) live vaccines.

The invention discloses a salmonella typhimurium bacteriophage T156 and application thereof. The bacteriophage is a broad-spectrum type, can split salmonella and a drug-resistant strain thereof, and is identified as a caudal bacteriophage long-tail bacteriophage family; and the bacteriophage T156 has stable titer with the pH of 3-12 and the temperature of 30-50 DEG C. The bacteriophage provided bythe invention can be used for effectively controlling salmonella typhimurium ATCC 13311 in a food sample, and has the characteristics of high specificity, no residue and safety compared with antibiotics and chemical preservatives.

The invention provides a traditional Chinese medicine preparation for controlling pullorosis salmonellosis. The traditional Chinese medicine preparation is characterized by being prepared from the following extracts in parts by weight: 3 to 5 parts of polygonum cuspidatum extract, 1 to 2 parts of fructus chebulae extract, and 1 to 2 parts of herba violae extract. The prepared traditional Chinese medicine compound particles are uniform in size, consistent in color and luster, and free of moisture adsorption, caking, deliquescence and other phenomena, meet the requirements of Chinese Pharmacopoeia, and are controllable in quality. The pharmacodynamics study shows that the traditional Chinese medicine preparation can be used for preventing and treating pullorosis salmonellosis.

The invention discloses a salmonella constant-temperature fluorescent detection primer combination capable of avoiding false negative, a reagent kit and a detection method. The salmonella constant-temperature fluorescent detection primer comprises a detection primer group and an internal standard primer group, wherein the detection primer group is designed according to a salmonella SPI gene, and a sequence of the detection primer group is as shown in SEQ ID NO.1 to 6; the internal standard primer group is designed according to a salmonella SPF gene, and a sequence of the internal standard group is as shown in SEQ ID NO.7 to 12. Besides the primer groups, the salmonella constant-temperature detection reagent kit also comprises a DNA polymerase, reaction liquid, an internal standard target fragment, a positive reference and a negative reference. The detection primer, the reagent kit and the detection method have advantages of rapidness, high efficiency, simplicity in operation, high specificity, high sensitivity, suitability for the on-site detection and the like, can effectively prevent the occurrence of false negative and are suitable for being popularized to use.

The invention discloses an attenuated recombinant bacterium, a preparation method thereof, application, and a tumor targeted medicine. RelA and spoT mutant salmonella typhimurium is adopted as a carrier, and a recombinant plasmid for encoding a ClyA gene is transferred into the relA and spoT mutant salmonella typhimurium so as to obtain the attenuated recombinant bacterium. The preparation methodcomprises the steps of constructing the recombinant bacterium, preparing the relA and spoT mutant salmonella typhimurium, and guiding. According to the attenuated recombinant bacterium, through the plasmid carrying encoding cytolysin, ClyA protein is over-expressed after a bacterium targeting tumor, tumor stroma cells and tumor cells are effectively killed through destroying the completeness of acell membrane, and the attenuated recombinant bacterium can be applied to preparing the tumor targeted medicine.

The invention discloses a salmonella pullorum and mycoplasma synoviae double-plate agglutination antigen as well as a preparation method and application thereof, and the preparation method comprises the following steps: respectively carrying out resuscitation passage on salmonella pullorum C79-1 and mycoplasma synoviae GX11-T to prepare seed bacterial liquid, inoculating the seed bacterial liquid into a corresponding culture medium, carrying out enlarged culture, harvesting, respectively inactivating by a formaldehyde solution, concentrating, mixing the two bacterial liquids according to a certain volume ratio, adding a crystal violet solution with the final concentration of 3% by mass fraction for dyeing and glycerol with the volume fraction of 10%, uniformly mixing, and subpackaging, thereby obtaining the product. The technological method is simple and reasonable, low in cost and good in stability, the prepared double-plate agglutination antigen can detect two epidemic disease antibodies at the same time through one-time reaction, the advantages of being high in sensitivity, rapid in diagnosis, high in specificity, easy to observe the agglutination effect and the like are achieved, the detection time and the detection cost are remarkably saved, and the detection efficiency is improved. The product can be clinically used for rapidly detecting the two antibodies of the pullorum disease and the mycoplasma synoviae, diseased chickens are eliminated, and disease purification is achieved.

The invention discloses a premix capable of reducing propagation and invasion of Salmonella Enteritidis phage type 4 in a laying hen. The premix comprises, by mass, ferrous fumarate, ferrous lactate, zinc methionine chelate, zinc propionate, manganese methionine chelate, copper sulphate, cobalt acetate, a sodium selenite premix, a potassium iodide premix, magnesium sulfate and calcium carbonate. The invention also discloses a preparation method for the premix capable of reducing propagation and invasion of Salmonella Enteritidis phage type 4 in the laying hen. The additive premix can provide trace element nutritional components needed by the laying hen and reduce the positive rate of almonella Enteritidis phage type 4 in laying hens of common chicken flocks; and the proportion of salmonella-positive eggs is reduced by 20 to 30%, and the course that the excrement of the laying hen carries Salmonella Enteritidis phage type 4 is shorted by 7 to 14 d.

The invention provides a method for detecting salmonella. The method comprises the following steps: (1) taking nucleic acid of a sample to be detected as a template, and carrying out amplification bya loop-mediated isothermal amplification technique by using specific primers of the salmonella, so as to obtain an amplified product; (2) under mediation of specific crRNA, subjecting the amplified product obtained in the step (1) to a cracking reaction by using a CRISPR system, so as to obtain a cracked product; and (3) carrying out chromogenic detection on the cracked product obtained in the step (2) by using a colloidal gold test paper strip. The method has very good sensitivity and accuracy.

The invention discloses a preparation method and a test method of a bacteriophage mixed preparation for killing salmonella, and relates to the technical field of veterinary sterilization preparations.The preparation method disclosed by the invention comprises the following steps: s01, performing induction and identification of mild salmonella bacteriophage; s02, purifying the salmonella bacteriophage; s03, storing the suspension; and s04, preparing phage cocktail; the test method comprises the following steps: t1, preparing a liquid culture medium; t2, diluting a bacterial liquid; t3, performing mixing preparation; and t4, performing reference test. For the salmonella with different concentrations, the preparation has a good bactericidal effect, can improve the bactericidal ability of thesalmonella compared with antibiotics and reduce the generation of bacterial resistance, and is a mixed preparation applied to environmental sterilization instead of the antibiotics.

The invention relates to a classification method of salmonella strains by using whole-cell fatty acid, which belongs to the bioengineering technical field. The method comprises the following steps: respectively culturing a plurality of salmonella strains, extracting strain cells, acquiring types and content of aliphatic acid in strain cells, drafting an aliphatic acid system dendrogram of salmonella strains according to types and content of aliphatic acid of salmonella; employing the above method to obtain the types and content of aliphatic acid of salmonella strains to be measured, comparing the types and content with the aliphatic acid system dendrogram of salmonella strains, and then classifying the salmonella strains to be measured. The method provided in the invention has the advantages of precise classification for salmonella strains, faster classification speed and low application cost.

The invention discloses an enterococcus faecium YQH2, and further discloses a probiotic preparation. The probiotic preparation comprises the enterococcus faecium YQH2. The invention also discloses application of the enterococcus faecium YQH2 or the probiotic preparation in breeding of poultry animals. The invention also discloses application of the enterococcus faecium YQH2 or the probiotic preparation in preparation of medicines for repairing intestinal injury or enhancing intestinal immunity. The invention further discloses application of the enterococcus faecium YQH2 or the probiotic preparation in preparation of amedicines for inhibiting salmonella typhimurium infection. The enterococcus faecium YQH2 disclosed by the invention can inhibit the growth of pathogenic bacteria. The enterococcus faecium YQH2 can improve the crypt depth of salmonella infection and increase of an inflammatory factor level, stimulates proliferation of intestinal mucosa epithelial cells and has the potential of maintaining intestinal mucosa barriers.