Salmonella typhimurium bacteriophage T156 and application thereof

A technology of Salmonella typhi and Salmonella, applied in the direction of phage, virus/phage, application, etc., can solve problems such as less research

Active Publication Date: 2020-11-17
HUAZHONG AGRI UNIV
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microencapsulation of phage can basically prevent the influence of the external environment on phage. At present, some microencapsulated phage products have appeared at home and abroad. In the food field, microencapsulation technology is mainly used in beverage production, bakery products, meat products and dairy products, etc. Many fields, but less research on the application of microencapsulated phage in food

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Salmonella typhimurium bacteriophage T156 and application thereof
  • Salmonella typhimurium bacteriophage T156 and application thereof
  • Salmonella typhimurium bacteriophage T156 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] The isolation and screening method of Salmonella typhimurium phage T156 (hereinafter referred to as: phage T156), the steps are:

[0068] (1) Sample collection

[0069] The sewage samples came from the underground sewage channel of a community in Wuhan City, Hubei Province.

[0070] (2) Screening of Salmonella phage

[0071]a. Coarsely filter the collected sewage with filter paper to remove large impurity particles. The filtered filtrate was placed in a 50mL centrifuge tube, centrifuged at 11000r / min at 4°C for 15min, and the supernatant was collected and filtered through a microporous membrane with a pore size of 0.22μm. The filtrate was the required sample and placed at 4°C for later use;

[0072] b. Inoculate Salmonella strain ATCC 13311 into TSB medium, shake culture at 37°C until logarithmic growth phase, take 2.5mL logarithmic phase bacterial liquid, 5mL filtrate and 10mL TSB liquid medium, mix well, place in a constant temperature shaker at 37°C Shake culture ...

Embodiment 2

[0082] Example 2: Determination of bacteriophage T156 host spectrum

[0083] Select Salmonella typhimurium ATCC 14028, ST-8, ATCC 13311, 30, 36, SJTUF 13336, SITUF13350, SJTU 13277, STTUF 13337, SJTUF 13306, 10855, UK 1, 114, 172, 206; Salmonella enteritidis ATCC 1, 114, 172, 206; , SJTUF 10984, 38, 39, 42, 10960, 11561, 201, 211, CMCC 50093, 4917, 4693; Salmonella pullorum CVCC 534, C79-3; Salmonella Dublin 3710, 3723; Salmonella paratyphi B CMCC 50094; Indiana Salmonella 13500, 13520; Salmonella Agora 17, 19, 21; Salmonella choleraesuis ATCC 10708; Salmonella duck CMCC 9270; Escherichia coli DH 5α, BL 21, T 10; Staphylococcus aureus ATCC 29213, 6538; A total of 46 strains of 19115 strains were used for host spectrum analysis.

[0084] Cultivate the above-mentioned bacterial strains (40 strains of Salmonella and 6 strains of other bacteria) to the logarithmic phase, and when the temperature of the upper layer agar (0.7% TSA) drops to 45°C, take 3.8 mL of the upper layer of a...

Embodiment 3

[0091] Electron Microscopic Observation of Example 3 Phage T156

[0092] Set the purified and concentrated phage suspension to ultracentrifugation at 4°C and 40,000r / min for 1 hour. After the centrifugation, the phage will precipitate at the bottom of the centrifuge tube, then resuspend with 0.1mol / L ammonium acetate, and pipette 20μL of the resuspension onto the parafilm Take the copper mesh lightly and place it in the phage suspension, soak it for 10 minutes, absorb the excess liquid with filter paper, then place the copper mesh in phosphotungstic acid dye with a volume fraction of 2% and pH=7 for 10 minutes, and then use the filter paper to remove the Slowly absorb excess liquid from the edge of the copper grid, and let it dry naturally until completely dry. The prepared copper grid was observed under a transmission electron microscope for phage morphology, and its size was measured with the software DigitalMicrograph Demo 3.9.1, and photographed and recorded.

[0093] The ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a salmonella typhimurium bacteriophage T156 and application thereof. The bacteriophage is a broad-spectrum type, can split salmonella and a drug-resistant strain thereof, and is identified as a caudal bacteriophage long-tail bacteriophage family; and the bacteriophage T156 has stable titer with the pH of 3-12 and the temperature of 30-50 DEG C. The bacteriophage provided bythe invention can be used for effectively controlling salmonella typhimurium ATCC 13311 in a food sample, and has the characteristics of high specificity, no residue and safety compared with antibiotics and chemical preservatives.

Description

technical field [0001] The invention relates to the field of food safety, in particular to a Salmonella typhimurium phage T156 and an application thereof. Background technique [0002] Foodborne diseases are diseases caused by ingesting food contaminated by microorganisms, toxic chemicals or other harmful substances. In recent years, foodborne diseases have gradually become the primary problem of global food safety, seriously affecting economic development, and endangering public health. . [0003] Salmonella is one of the most common causes of food-borne diseases worldwide, ranking first in the world of food-borne pathogens, and food safety problems frequently occur in many developed countries. Salmonella infection can be divided into 4 types: enteritis type, typhoid fever type, sepsis type and local suppurative infection. The incubation period is 4-48h. After a person is infected with Salmonella, symptoms such as loss of appetite, vomiting, diarrhea, and loose stool will ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A01N63/40A01P1/00C12R1/92
CPCC12N7/00A01N63/40C12N2795/10321Y02A50/30
Inventor 王小红李亚萌王佳李俊杰丁一峰张宇
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap