35 results about "Salmonella Paratyphi" patented technology

The invention discloses a salmonella phage RDP-NSA-19050 with a wide lysis spectrum, wherein the preservation number is CGMCC No.21415, the gene length is 87591 bp; after sequencing is carried out on whole genes, it is found that the salmonella phage RDP-NSA-19050 does not have virulence genes and lyogenic genes, and the salmonella phage RDP-NSA-19050 has a good lysis spectrum. The salmonella bacteriophage RDP-NSA-19050 provided by the invention can be used for cracking a plurality of pathogenic bacteria such as salmonella steulani, salmonella gallinarum, salmonella typhimurium, salmonella paratyphi A, salmonella paratyphi B, salmonella typhimurium, salmonella enteritidis and salmonella kkinensis; the salmonella bacteriophage RDP-NSA-19050 has the characteristics of wide splitting spectrum and high splitting rate, can be independently used, can also be compounded with bacteriophages of other escherichia coli and salmonella and probiotics for use, and can effectively reduce the death rate of chicken infected with salmonella.

The invention discloses a bacterial polysaccharide protein conjugate vaccine using a hepatitis B surface antigen as carrier protein and a preparation method of the bacterial polysaccharide protein conjugate vaccine. According to the vaccine, protein is the hepatitis B surface antigen, and a bacterial polysaccharide is selected from any one or more of a haemophilus influenza type b polysaccharide, group A, group C, group Y and group W135 meningococcal polysaccharides, a salmonella typhi type Vi polysaccharide, a group B streptococcus type Ia polysaccharide and the like, pneumococcus serotype type 1, 2 and the like, and salmonella paratyphi type A or salmonella paratyphi type B. Animal experiments show that the antibody positive conversion rates of the bacterial polysaccharide and the hepatitis B surface antigen in the vaccine are both more than 85%, so that the vaccine is relatively high in antibody positive conversion rate; carrier protein plays a role in transforming the bacterial polysaccharide from a T-cell-independent antigen into a T-cell-dependent antigen, and also can be used for preventing diseases caused by hepatitis B virus; and by adopting the bacterial polysaccharide protein conjugate vaccine disclosed by the invention, the problem of performing immunization inoculation on infants and young children under 2 years old can be solved, the function of one injection with multiple immune effects also can be achieved, and the use crowd and coverage rate of the vaccine can be expanded.

The invention belongs to the field of microbiological detection and discloses a method for detecting live salmonella typhimurium, salmonella paratyphi b and salmonella typhi in food simultaneously and rapidly. A special primer is designed aiming at salmonella typhimurium, salmonella paratyphi b and salmonella typhi, hydrazoic brominated c ingot processing is adopted to remove interference of dead bacteria, and finally deoxyribonucleic acids (DNAs) are extracted to perform multiplex polymerase chain reaction. Compared with a classical bacterium separating and identifying method and a serologic typing method, the method for detecting live salmonella typhimurium, salmonella paratyphi b and salmonella typhi in the food simultaneously and rapidly is rapid and accurate.

The invention belongs to the field of biotechnology and medical science, and provides a typhoid fever salmonella and salmonella paratyphi fluorescent quantitative polymerase chain reaction (PCR) detection kit. The detection kit comprises a first reaction solution, a second reaction solution and paraffin, wherein the first reaction solution comprises a reaction buffer solution, a primer pair and an ecprobe aiming at typhoid fever salmonella, a primer pair and an ecprobe aiming at the salmonella paratyphi. The sequence of the primer pair aiming at the typhoid fever is SEQ ID NO: 1 and SEQ ID NO: 2, the sequence of the ecprobe aiming at the typhoid fever is SEQ ID NO: 3, the sequence of the primer pair aiming at the salmonella paratyphi is SEQ ID NO: 3 and SEQ ID NO: 3, and the sequence of the ecprobe aiming at the salmonella paratyphi is SEQ ID NO: 6. The second reaction solution comprises deoxyribonucleic acid (DNA) polymerase O. The invention further provides an application of the detection kit in detection of the typhoid fever salmonella and salmonella paratyphi. According to the detection kit, the detection time is shortened, cross contamination among samples is avoided, and the result is accurate and reliable.

The invention discloses primers, probes, a test kit and a test method for triple real-time fluorescence PCR detection of Salmonella choleraesuis, Salmonella paratyphi, Salmonella typhi, and Salmonella gallinarum. According to the present invention, a triple real-time PCR method is used, wherein a consensus sequence of Salmonella choleraesuis and Salmonella paratyphi is used for the detection of Salmonella choleraesuis and Salmonella paratyphi, a specific sequence of Salmonella typhi is used for the detection of Salmonella typhi, and a specific sequence of Salmonella gallinarum is used for the detection of Salmonella gallinarum. Whether a sample is contaminated by Salmonella choleraesuis, Salmonella paratyphi, Salmonella typhi, and Salmonella gallinarum is determined via a real-time fluorescence PCR amplification, and then Salmonella choleraesuis and Salmonella paratyphi are distinguished via a single fluorescent PCR amplification; the detection is rapid, the process of preparing sample and issuing test results can be finished in 31hours, the results are reliable, and sensitivity and specificity are high.

The invention discloses a salmonella paratyphi ompN gene prokaryotic expression system and application of recombination expression protein thereof, and belongs to the technical field of biologics. The nucleotide sequence of the salmonella paratyphi ompN gene is shown in the SEQ ID No: 1. The amino acid sequence of the recombination expression protein of the salmonella paratyphi ompN gene is shown in the SEQ ID NO: 2. The salmonella paratyphi ompN gene disclosed by the invention is wide in distribution and conservative in sequence, the recombination expression product rOmpN has strong immunogenicity and immunoprotection, and can be used as multivalent salmonella paratyphi genetic engineering vaccine candidate antigen.

The invention provides compositions and methods for preventing, treating and diagnosing infection by Salmonella enterica serovar typhi (S. typhi) and/or S. paratyphi, i.e., typhoid fever.

The invention relates to fermentation production and preparation of an active product, belongs to the field of bioengineering and in particular relates to a prodigiosin (PG) producing strain as well as a preparation method and an application thereof. The PG producing strain is Proteus Vulgaris and is preserved on June 24th, 2013 in CGMCC (China General Microbiological Culture Collection Center) and has the preservation number of CGMCCNo.7815 and the preservation unit address of No.3 No.1 Courtyard, West Beichen Road, Chaoyang District, Beijing, Institute of Microbiology of Chinese Academy of Sciences. The PG producing strain can produce PG through fermentation, and an improved inorganic salt culture medium is adopted and is cultured for 18-24 hours at a culture temperature of 20-28 DEG C and a rotating speed of 150-300rpm, and yield of PG can be 80-120mg/L. The produced prodigiosin has an inhibitory activity to staphylococcus aureus, salmonella paratyphi, tritirachium album and pseudomonas aeruginosa and has a good application prospect in bio-preparation of the PG.

The invention provides three specific target genes for detecting salmonella paratyphi, a corresponding PCR primer pair and a method for detecting the target genes by using the primer pairs. The PCR primer pair is designed according to the three specific target genes for detecting salmonella paratyphi, the specific target genes are high in stability and strong in specificity, and through reasonably optimizing a PCR system, and adopting gel electrophoresis detection means, salmonella paratyphi can be quickly and accurately identified. The detection method provided by the invention comprises the following steps: (1) extracting the DNA of a sample, and carrying out PCR amplification; (2) detecting an amplified product through gel electrophoresis; and (3) comparing rear electrophoretic bands, if the bands exist at 523 bp, 212 bp and 169 bp positions, proving that the sample contains salmonella paratyphi. According to experimental comparison and analysis, the method disclosed by the invention has the characteristics of strong singularity, reliable detection results, and simple result determination, and can be widely applied in the field of food sanitation.

The present invention relates to a new cocktail of bacteriophages with specific lytic activity against Salmonella enteritidis, Salmonella typhimurium and Salmonella paratyphi B., for reducing, eliminating and/or preventing them in farm animals and animals from the poultry sector, such as poultry, hens and breeding hens, in addition to eggs. It may be administered as an additive in the feed, in water or by spray. Moreover, the cocktail may be used as a disinfectant in work areas of farms and abattoirs, and in processed foods, without affecting the organoleptic properties of the product.

The invention belongs to the technical field of fermentation engineering and in particular relates to a prodigiosin producing strain and a method for producing prodigiosin through fermentation of the prodigiosin producing strain. The prodigiosin producing strain is acid producing klebsiella (Klebsiella oxytoca), is preserved on June 24th, 2013 in CGMCC (China General Microbiological Culture Collection Center) and has the preservation number of CGMCCNo.7816 and the preservation unit address of No.3 No.1 Courtyard, West Beichen Road, Chaoyang District, Beijing, Institute of Microbiology of Chinese Academy of Sciences. The prodigiosin producing strain can produce the prodigiosin through fermentation, an improved inorganic salt culture medium is adopted and is cultured away from light for 20-26 hours at a temperature of 24-28 DEG C and a rotating speed of 250-360rpm, and pigment yield can be 70-90mg/L. The produced prodigiosin is sensitive to natural light and ultraviolet rays and has an inhibitory activity to staphylococcus aureus, salmonella paratyphi and tritirachium album. The prodigiosin producing strain has a good application prospect in fermentation production of the prodigiosin and the fields of tumour suppression, food, cosmetics and the like.

The invention discloses a bee venom antimicrobial preparation and a preparation method thereof. The bee venom antimicrobial preparation is prepared from, by weight, 15-25% of chlorhexidine acetate, 5-10% of a bee venom source solution, 0.7-1.5% of a red date extracting solution, 0.2-0.5% of a rose flower extracting solution, 0.4-0.6% of a honeysuckle flower extracting solution, 0.3-0.8% of vanillyl butyl ether, 0.7-0.9% of propylene glycol and 50-70% of purified water. Accordingly, related symptoms caused by meridian obstruction are relieved, and good inhibiting and killing effects on germs and inflammations of the surface of the skin are achieved; by adding the rose flower extracting solution, the probability that a user has allergy can be effectively lowered, the immune function of the user is improved, and the disease resistance is enhanced; by adding the honeysuckle extracting solution, the certain inhibiting effect can be achieved on staphylococcus aureus, hemolytic streptococcus,escherichia coli, shigella dysenteriae, vibrio cholera, typhoid bacilli, salmonella paratyphi and the like.

The invention discloses salmonella paratyphi A polysaccharide modified nanoparticles and application thereof. The nanoparticles are salmonella paratyphi A polysaccharide modified protein. The proteinis a) or b) or c) or d) as follows: a) protein composed of an amino acid sequence as shown in SEQ ID No.3; b) fusion protein obtained by fusing a protein label at a carboxyl terminal or/and an amino terminal of the protein shown in a); and c) protein obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence as shown in SEQ ID No.3. Animal experiments show that the antibody level and the protection effect are obviously improved compared with those of single polysaccharide and polysaccharide connected with CTB. It is indicated that a nanoscale polysaccharide conjugate vaccine with higher potential can be prepared by using a biological method, and a direction is provided for the development of the polysaccharide conjugate vaccine in thenext step.

The invention provides a nucleic acid, a kit and a detection method for detecting pigeon salmonella paratyphi. The invention provides a primer pair for detecting pigeon salmonella paratyphi and an internal reference primer pair for detecting salmonella. The size of the amplified fragments is 613 bp for pigeon salmonella paratyphi and 463 bp for salmonella. A rapid and accurate PCR detection methodfor detecting pigeon salmonella paratyphi is established in the invention, and the method can accurately detect clinical suspicious strain samples, distinguish other common pathogenic salmonella serotypes, and the amplified results of non-salmonella pathogenic bacteria are negative. The detection method provided by the invention has the advantages of high specificity, high sensitivity, short detection time, simple operation, easy judgment and low requirements on equipment, and only needs half a day to complete the detection and plays a very important role in the diagnosis and control of epidemic situation, thereby minimizing economic losses and harm to public health.