7 results about "Salmonella Paratyphi" patented technology

The application discloses a kind of biological sensor of nucleic acid aptamer-based paratyphoid A salmonella and preparation method thereof, it is introduced 3 fluorine atoms (F) modification in the 5' end, 3' end and intermediate position of aptamer 2'F3-(5'3'mDNA) sequence (SEQ ID NO:1), significantly enhance the binding force of aptamer and graphene, significantly improve the signal stability and anti-interference ability of biosensor, and can effectively block non-specific fluorescence leakage, provide reliability guarantee for complex sample detection.The application is matched with graphene oxide quenching substrate, realizes the high sensitivity detection of paratyphoid A salmonella, the detection limit of the sensor reaches 10 cells / mL within 5 min, the specificity to complex sample is >93.4%, and it is suitable for food safety rapid screening and clinical diagnosis.

The invention provides a salmonella paratyphi A strain for recombining and expressing Vi polysaccharide, which is characterized in that a viaB operon for expressing the Vi polysaccharide from salmonella paratyphi is cloned into a salmonella paratyphi A genome by a genetic engineering technology, so that the expression of the Vi polysaccharide in the salmonella paratyphi A is realized; furthermore, a constitutive strong promoter (Tac promoter) is used for replacing a viaB natural promoter to realize high expression of the Vi polysaccharide under an in-vitro culture condition; further, the msbB gene of the strain is knocked out to reduce the acylation level of the LPS and reduce the toxicity of the LPS; the vesicle vaccine prepared from the strain can induce immune protection effects on two serotypes.

The present invention relates to immunogenic compositions comprising Salmonella enterica paratyphoid A serotype outer membrane vesicles (OMV), vaccines comprising said immunogenic compositions, and methods and uses of said immunogenic compositions. The invention also relates to Salmonella paratyphi A bacteria comprising the modified lipid A.

The present invention discloses a pharmaceutical composition, its manufacturing method, and its use, wherein the pharmaceutical composition comprises a first active ingredient, a second active ingredient, and a pharmaceutically acceptable carrier or excipient, the first active ingredient being a microbial agent containing one or more of Staphylococcus aureus, Bordetella pertussis, diphtheria toxoid, tetanus toxoid, Salmonella typhi, or Salmonella paratyphi, and the second active ingredient comprising polyinosinic acid, polycytidylic acid, and vitamins. The pharmaceutical composition is an artificial active immunotherapy for tumors, which, by "stimulating" the entire immune system, activates the human immune system using bacteria to kill cancer cells, making the treatment extremely stable and reliable, significantly saving and extending the lives of cancer patients, while simultaneously being extremely safe, having very few toxic side effects, and having low manufacturing costs.

The invention discloses bacteriocin AM12LCIr as well as a preparation method and application of the bacteriocin AM12LCIr. The invention provides a bacteriocin AM12LCIr, the coding sequence of the bacteriocin AM12LCIr is composed of 46 amino acids, the relative molecular weight is 5067.73 Da, homology analysis shows that the bacteriocin AM12LCIr has the highest homology with class II bacteriocin LCI bacteriocin, but the AM12LCIr and LCI have obvious differences in structure and physicochemical properties, and the bacteriocin AM12LCIr belongs to a novel bacteriocin. According to the present invention, the bacteriocin AM12LCIr is subjected to heterologous expression, and the prepared recombinant protein has good inhibition effect on a variety of bacteria, can inhibit Escherichia coli, Listeria monocytogenes, Enterobacter aerogenes, Salmonella paratyphi B, Staphylococcus aureus and Bacillus cereus, has broad-spectrum antibacterial activity, can be used for preparing more antibacterial products, and has broad application prospects. The invention has important significance for clinically preventing and treating a plurality of pathogenic bacteria including escherichia coli.

The invention discloses a salmonella paratyphi targeting antibacterial peptide as well as a preparation method and application thereof, and belongs to the field of bioengineering, and the sequence of the antibacterial peptide is as shown in SEQ ID No.1; the invention further discloses application of the antibacterial peptide targeting the salmonella paratyphi to preparation of a medicine for treating salmonella paratyphi infectious diseases. The antibacterial peptide disclosed by the invention has efficient targeted bactericidal activity on salmonella paratyphi, and has no toxicity on human red blood cells, so that the antibacterial peptide has the potential of becoming a feed antibiotic substitute.

The invention discloses a salmonella paratyphi A biosensor based on a nucleic acid aptamer and a preparation method of the salmonella paratyphi A biosensor. Three fluorine atoms (F) are introduced into the 5'end, the 3 'end and the middle position of an aptamer 2' F3-(5 '3'm DNA) sequence (SEQ ID NO: 1) for modification, so that the binding force of the aptamer and graphene is remarkably enhanced, the signal stability and the anti-interference capability of the biosensor are remarkably improved, non-specific fluorescence leakage can be effectively blocked, and reliable guarantee is provided for complex sample detection. According to the present invention, with the cooperation of the graphene oxide quenching substrate, the high sensitivity detection of the salmonella paratyphi A is achieved, the detection limit of the sensor within 5 min can achieve 10 cells / mL, the sensitivity is high, the specificity on the complex sample is more than 93.4%, and the method is suitable for food safety rapid screening and clinical diagnosis.