81 results about "Salmonella typhosa" patented technology

A breast milk-derived Limosilactobacillus fermentum MSJK0025 capable of improving the intestinal flora condition and the use thereof. The breast milk-derived Limosilactobacillus fermentum MSJK0025 is deposited in the China General Microbiological Culture Collection Center with a deposit number of CGMCC No. 26426. The breast milk-derived Limosilactobacillus fermentum is a new strain isolated from breast milk of healthy puerperae, and is referred to as Limosilactobacillus fermentum MSJK0025. The strain has a good antibiotic sensitivity and stable inheritance of traits, has a good tolerance to acid, bile salts and artificial gastric / intestinal fluid, and has a fast growth rate and good safety. Furthermore, the strain has a strong intestinal colonization capability and can effectively inhibit intestinal pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Candida albicans, but has no inhibitory effects on intestinal probiotics such as Lactobacillus and Bifidobacterium.

The invention discloses a polypeptide drug for resisting gram-negative bacteria and application, and belongs to the technical field of biological medicine. The MIC of the antibacterial peptide obtained through screening on escherichia coli, salmonella typhimurium, pseudomonas aeruginosa, klebsiella pneumoniae, shigella sonnei and stenotrophomonas maltophilia is 16 [mu] g / mL, 2 [mu] g / mL, 16 [mu] g / mL, 4 [mu] g / mL, 2 [mu] g / mL and 4 [mu] g / mL respectively, and the antibacterial peptide has broad-spectrum antibacterial performance. The hemolytic activity and toxicity of the compound are lower than therapeutic concentration threshold values, and the compound can be used for preparing drugs or medical products for treating multi-drug-resistant bacterium infection.

The invention belongs to the technical field of biology, and discloses a preparation method of fusion protein of salmonella typhimurium flagellin and Seneca virus antigen. Escherichia coli Rosetta (DE3) is selected as a host, a salmonella typhimurium flagellin gene and a Seneca virus antigen gene are fused and are cloned to a pET-28a (+) vector after codon optimization, the obtained fusion protein keeps the flagellin adjuvant activity, the solubility and the expression level of VP2 are improved, the yield can reach 40 mg / L, and the fusion protein can be used for preparing a recombinant vector. The expression and purification process cost is greatly reduced. The fusion protein provided by the invention can activate a TLR5 pathway and mouse macrophages, is simple in production process and low in cost, and has important significance on research and production of Seneca virus subunit vaccines.

The invention discloses an application of 3-methylindole in preparation of drugs for inhibiting salmonella typhimurium, which inhibits the expression of QS related genes and virulence genes and inhibits the formation of biofilms through the combination of 3-methylindole and LsrB protein, thereby reducing the pathogenicity of salmonella typhimurium. And the 3-methylindole can inhibit invasion and adhesion of salmonella typhimurium to cells, so that the 3-methylindole can be applied to preparation of the salmonella typhimurium inhibiting medicine.

The invention relates to the technical field of probiotic development and utilization, in particular to a goose source Weissella cibaria F11 and application thereof. The Weissella cibarius F11 is preserved in the China General Microbiological Culture Collection Center on July 11, 2025, and the preservation number is CGMCC NO.35211. The Weissella cibarius F11 has the characteristics of good acid resistance and cholate resistance, and can be used for preparing the acid-resistant and cholate-resistant strain. And the bacillus subtilis has a good inhibition effect on pathogenic bacteria such as escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and salmonella typhimurium, and also has stable acid production capacity and good safety. The Weissella sinus F11 provided by the invention meets the probiotic characteristic standard, the antibiotic resistance spectrum of the Weissella sinus F11 meets the feed probiotic safety standard, the growth of goslings can be remarkably promoted, the death rate and diarrhea occurrence rate of the goslings can be remarkably reduced, and the weissella sinus F11 can be used for preparing feed probiotics. The development potential in the fields of preparation of bacteriostatic and antibacterial drugs, probiotics for poultry feed, feed additives, poultry feed and the like is huge.

Provided are a Salmonella engineered cell-penetrating bacteriophage, and a construction method therefor and a use thereof. The Salmonella engineered cell-penetrating bacteriophage comprises a bacteriophage and a cell-penetrating peptide, the surface of the bacteriophage has a surface protein, the surface protein is fused with the cell-penetrating peptide, and the surface protein is a protein containing an Ig-like domain. By means of a bioinformatics method, proteins which are suitable for fusion expression with foreign proteins, namely proteins containing Ig-like domains, are screened out in a high-throughput manner from structural proteins of a Salmonella typhimurium bacteriophage, and then, fusion expression of a cell-penetrating peptide is implemented on the surface of the Salmonella typhimurium bacteriophage to obtain an engineered bacteriophage capable of efficiently killing Salmonella typhimurium in cells, thereby increasing the titer of the Salmonella typhimurium bacteriophage entering cells, and extending phage therapy to treatment of intracellular infections.

The invention discloses a strain of Shenjing pig combined lactobacillus and application thereof. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.31452. The strain is separated and screened from vaginal secretions of healthy women at the childbearing age and is sensitive to various common antibiotics, and fermented supernate of the strain shows relatively strong inhibition capability on escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, salmonella typhimurium, salmonella paratyphi B and gardnerella vaginalis; the compound has strong adhesion ability, growth inhibition ability and apoptosis promoting effect on human cervical cancer cells. When the strain is used for fermenting wall-broken ganoderma lucidum spore powder, the content of amino acid and bioactive substances such as peptides, arachidonic acids, steroids and polyketones can be greatly increased, the strain has an obvious inhibition effect on DSS-induced mouse colitis, and the inflammatory factor level is remarkably reduced. The strain not only has significant application value in biological agents, but also opens up a new way for combined application of lactic acid bacteria and ganoderma spores.

This invention discloses a method for detecting *Salmonella typhimurium* based on magnetic covalent organic frameworks and aptamer-modified gold nanoparticles, belonging to the field of food safety technology. The method uses a magnetic covalent organic framework as a capture probe to capture *Salmonella typhimurium* present in the detection system. After incubation, the magnetic covalent organic framework-*Salmonella typhimurium* complex is collected by magnetic separation. Then, aptamer-modified gold nanoparticles are added, and after incubation, the complex formed by the magnetic covalent organic framework-*Salmonella typhimurium* complex and the aptamer-modified gold nanoparticles is collected by magnetic separation. Finally, a TPE-4A / hydrogen peroxide substrate solution is added, and the absorbance and / or fluorescence intensity are measured. Color parameters are extracted using a smartphone. This method is simple to operate, highly sensitive, and highly specific. Three detection modes work together for self-testing, resulting in accurate and reliable results.

This invention belongs to the field of molecular biology detection technology, specifically relating to a primer and probe combination for detecting avian Salmonella based on the RAA-LFD method and its application, particularly to a primer and probe combination for detecting three avian serotypes of Salmonella: Salmonella pullorum, Salmonella enteritidis, and Salmonella typhimurium, based on the RAA-LFD method and its application. This invention designs different types of probes and primers targeting the genes of serotypes SP, SE, and STM of Salmonella. Using the primer and probe combination of this invention, rapid, sensitive, and specific detection of Salmonella serotypes and the three avian Salmonella serotypes SP, SE, and STM can be achieved, greatly shortening the detection cycle of avian salmonellosis and improving the detection efficiency and accuracy of avian salmonellosis.

A novel bacteriophage having bacteriolytic activity against bacteria of the genus Salmonella, such as S. Enteritidis, and a bacteriolytic agent consisting of the same are provided. The bacteriophage and the bacteriolytic agent including the bacteriophage have a wide host range for bacteria of the genus Salmonella. A host-specific bacteriophage and an effective Salmonella bacteriolytic agent consisting of the same are provided. A bacteriophage capable of effectively controlling S. Typhimurium, and in particular, multidrug-resistant S. Typhimurium, and a bacteriolytic agent consisting of the same are also provided. A bacteriophage having a specific genomic DNA sequence; a Salmonella bacteriolytic agent consisting of the bacteriophage; and a composition comprising the same are provided.

The invention discloses a lactobacillus jensenii strain and application thereof in fermentation of wall-broken ganoderma lucidum spore powder. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.31484. The strain is separated and screened from vaginal secretions of healthy women at the childbearing age, is sensitive to various common antibiotics, and has strong lactic acid production capacity; a fermentation supernatant of the bacillus subtilis shows relatively strong inhibition capability on escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, salmonella typhimurium, shigella dysenteriae, yersinia enterocolitica and gardnerella vaginalis, and has relatively strong adhesion capability, growth inhibition capability and apoptosis promotion effect on human cervical cancer cells. When the strain is used for fermenting wall-broken ganoderma lucidum spore powder, the content of amino acid and bioactive substances such as peptides, arachidonic acids, steroids and polyketones can be greatly increased, the strain has an obvious inhibition effect on DSS-induced mouse colitis, and the inflammatory factor level is remarkably reduced. The strain not only has significant application value in biological agents, but also opens up a new way for combined application of lactic acid bacteria and ganoderma spores.

The invention discloses an application of bilobalide in preparation of a product for resisting salmonella typhimurium infection. The bilobalide takes salmonella typhimurium QseB protein as a target, has a good interference effect on an AI-2 quorum sensing signal of salmonella typhimurium and has good pharmacological activity, and particularly, the bilobalide has good pharmacological activity under the condition that the growth of salmonella typhimurium is not inhibited. The motility, biofilm formation and infection pathogenicity of salmonella typhimurium are obviously inhibited. The bilobalide does not influence the growth of salmonella typhimurium, directly acts on pathogenic factors, weakens the pathogenicity of bacteria from the source, does not generate selective pressure on salmonella typhimurium, and does not cause the generation of drug-resistant pathogenic bacteria. Therefore, the bilobalide has a good application prospect in the aspect of development of the salmonella typhimurium infection resisting medicine.

The invention discloses a preparation method of a Salmonella typhimurium aptamer and a colorimetric probe thereof, and belongs to the technical field of food safety. The DNA sequence of the Salmonella typhimurium aptamer is shown in SEQ ID No: 1. The steps of using the Salmonella typhimurium aptamer for the preparation of the colorimetric probe are as follows: (1) preparing a gold nanoparticle solution with tetrachloroauric acid solution and distilled water; (2) preparing a Salmonella typhimurium aptamer solution with distilled water; (3) mixing the gold nanoparticle solution and the Salmonella typhimurium aptamer solution in a volume ratio of 5:1.8 to prepare a colorimetric probe solution; the ultraviolet absorption wavelength of the gold nanoparticles modified by the nucleic acid aptamer in the colorimetric probe solution is 523nm. It has been verified that compared with the existing mature nucleic acid aptamers, the colorimetric probe prepared by the invention has a higher linear fitting degree and a lower detection limit; at the same time, the screening method is simple and convenient, and rapid screening can be achieved by only building a simple server.

The present invention provides compositions and methods of inducing an immune response in a subject in need thereof, comprising administering to the subject an immunologically-effective amount of a live Salmonella typhi vector comprising a heterologous antigen from a pathogen, wherein the heterologous antigen comprises an outer membrane protein, an antigenic fragment thereof or a variant thereof, wherein the antigen is delivered to a mucosal tissue of the subject by an outer membrane vesicle.

The invention relates to the field of biological detection, in particular to a primer probe set, a detection reagent and application of the primer probe set and the detection reagent in detection of virulent pathogenic bacteria. The invention provides a primer probe group. The primer probe group comprises a primer probe group used for amplifying bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum-like bacteria, brucella, vibrio cholerae, salmonella typhimurium and staphylococcus aureus, wherein the primer probe group is used for amplifying bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum-like bacteria, brucella, vibrio cholerae and salmonella typhimurium. The invention establishes a primer probe combination for rapidly and sensitively detecting various virulent pathogenic bacteria and a kit thereof, aiming at eight virulent pathogenic bacteria such as bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum, vibrio cholerae, brucella, salmonella typhimurium and staphylococcus aureus. The kit is stored in a freeze-dried state, adaptive sample lysate can directly expand different types of samples, and the kit has the advantages of being good in specificity, high in sensitivity, short in detection time, good in accuracy, good in stability after redissolution and good in compatibility.

The invention belongs to the technical field of food safety, and relates to application of peanut red skin proanthocyanidin nano-liposome in inhibition of salmonella typhimurium, and the peanut red skin proanthocyanidin nano-liposome causes cell death by causing permeability change of cell membranes and cell walls of salmonella typhimurium. According to the present invention, the inherent limitation of the natural antibacterial substance PSPc is overcome through the efficient delivery system of the nano-liposome, such that the salmonella typhimurium inhibition activity of the nano-liposome can be completely performed and durably maintained, the method is efficient and safe, the application potential in the food preservation aspect is provided, and the value-added utilization of the peanut resource is improved.

The invention relates to the technical field of microorganisms, in particular to Eubacterium mucilaginosus E11405 and application thereof in bacteriostasis and anti-inflammation. The preservation number of the mucus eubacterium is CGMCC (China General Microbiological Culture Collection Center) No.31231. The application comprises inhibition of microorganisms and anti-inflammation. The microorganisms comprise one or more of listeria monocytogenes, staphylococcus aureus, salmonella typhimurium or escherichia coli. The Eubacterium mucilaginosus provided by the invention can relieve various infection symptoms caused by salmonella infection, and shows the potential in preparation of drugs for treating and / or preventing infection of pathogenic bacteria such as salmonella. In addition, the Eubacterium mucilaginosus provided by the invention can also effectively relieve inflammatory bowel diseases and relieve intestinal mucosa injury and colitis symptoms, provides a new strategy for preventing and treating inflammatory bowel diseases, and has potential clinical application prospects.

This invention relates to the field of microbial technology, and particularly to a non-lactolytic streptococcus and its application in the preparation of anti-Salmonella infection agents. This invention provides a non-lactolytic streptococcus, wherein the non-lactolytic streptococcus (… Streptococcus alactolyticus The strain was deposited at the China Center for Type Culture Collection (CCTCC) on March 30, 2026, with accession number CCTCC NO: M 2026565. Pre-gavage administration of the described non-lactose-degrading Streptococcus significantly enhanced the host's resistance to Salmonella typhimurium. Host weight loss was slowed after Salmonella typhimurium infection, and the amount of Salmonella typhimurium in the liver and spleen was suppressed, reducing the host's pathogenic load.

The invention belongs to the technical field of microbiological detection, and discloses an RPA (recombinase polymerase amplification) primer pair, a real-time fluorescent RPA primer group and a kit for detecting salmonella typhimurium. The primer pair for detecting the salmonella typhimurium based on the RPA comprises an upstream primer RPA-F with a sequence as shown in SEQ ID NO: 1 and a downstream primer RPA-R with a sequence as shown in SEQ ID NO: 2, and the primer group for detecting the salmonella typhimurium based on the real-time fluorescence RPA comprises the upstream primer RPA-F, the downstream primer RPA-R and a probe RPA-P with a sequence as shown in SEQ ID NO: 3. The primer group and the primer pair can be used for rapidly detecting salmonella typhimurium, and are high in specificity and sensitivity and low in minimum detection limit.

The present disclosure relates to the technical field of medicine, and in particular to a pharmaceutical composition, preparation method and use thereof. The pharmaceutical composition includes a first active ingredient, a second active ingredient, and a pharmaceutically acceptable carrier or excipient. The first active ingredient is a microbial agent, including one or more of Staphylococcus aureus, Bordetella pertussis, diphtheria toxoid, tetanus toxoid, Salmonella typhi, and Salmonella paratyphi. The second active ingredient includes polyinosinic acid, polycytidylic acid, and vitamin. The pharmaceutical composition of the present disclosure pertains to an artificial active immunotherapy for tumors. It can “stimulate” the entire immune system, making the therapy of using bacteria to activate the human immune system to kill cancer cells highly stable and reliable. This composition can significantly save and prolong the lives of cancer patients while exhibiting extremely high safety, minimal toxic side effects, and low production costs.

Provided herein are methods and compositions for detecting Salmonella Typhimurium in a sample.

A PCR diagnostic assay, methods and a kit for differentiating between wild-type virulent Salmonella Typhimurium and vaccine-associated Salmonella Typhimurium in a subject vaccinated against Salmonella Typhimurium.

The application belongs to the technical field of biology, and discloses a preparation method of a Salmonella typhimurium flagellin and Senecavirus antigen fusion protein. The application selects Escherichia coli Rosetta (DE3) as a host, fuses a Salmonella typhimurium flagellin gene and a Senecavirus antigen gene, optimizes codons, and then clones the fusion protein into a pET-28a (+) vector, so that the obtained fusion protein can keep the adjuvant activity of the flagellin, improve the solubility and expression level of VP2, and the yield can reach 40 mg / L, thereby greatly reducing the expression and purification process cost. The fusion protein provided by the application can activate the TLR5 path and activate mouse macrophages, and the production process is simple and low in cost, so that the application has important significance for the research and production of Senecavirus subunit vaccines.

The present invention discloses a strain of poultry-derived Salmonella typhimurium ST0602 and its application. The strain has been preserved, and the preservation unit is: China Center for Type Culture Collection; address: Wuhan University; preservation date: May 30, 2024; preservation number: CCTCC NO.M 20241100. The present invention also provides the application of the Salmonella typhimurium for constructing a poultry attenuated vaccine strain. Salmonella typhimurium ST0602 is sensitive to currently common antibacterial drugs and has good genetic stability, and is suitable for constructing a live attenuated vaccine strain of Salmonella typhimurium.

Provided is use of a combination of three or more of the following microorganisms in the preparation of an anti-tumor combination vaccine: Bordetella pertussis, Salmonella typhi, Salmonella paratyphi A, Salmonella paratyphi B, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Proteusbacillus vulgaris, Lactic acid bacteria, Bifidobacterium longum, Bordetella pertussis, Corynebacterium diphtheriae and Clostridium tetani, Clostridium acetobutylicum, Salmonella typhimurium, and Streptococcus pyogenes. The anti-tumor combination vaccine is broad-spectrum, safe and non-toxic, and thus has the prospect of clinical applications.

The invention belongs to the field of biological medicines, and particularly relates to a drug-loaded hybrid membrane system for targeting tumor-associated macrophages as well as a preparation method and application of the drug-loaded hybrid membrane system. The invention discloses a hybrid membrane carrier for targeting tumor-associated macrophages. The hybrid membrane carrier is formed by fusing attenuated salmonella typhimurium ghost and an M2pep peptide modified macrophage membrane. The invention also discloses a drug-loaded hybrid membrane system for targeting tumor-associated macrophages. The drug-loaded hybrid membrane system comprises a hybrid membrane carrier and a drug which is loaded in the hybrid membrane carrier and is used for immunoregulation / tumor resistance. The drug-loaded hybrid membrane system provided by the invention integrates the advantages of active targeting, homologous targeting, macrophage specific targeting, biocompatibility, immune activation efficiency and the like, and develops a new treatment mode for malignant tumors with low immunotherapy effect rate.

The invention relates to the technical field of microorganisms, in particular to bacillus altitudinis MLF-1 and a complex microbial inoculant and application thereof.The bacillus altitudinis strain MLF-1 is obtained by being separated from healthy cattle rectum contents by a research group, and it is detected that the strain has the good beta-1, 4-glucanase producing capacity, and the beta-1, 4-glucanase producing capacity is high; the antibacterial effect is achieved on enterobacter aerogenes, staphylococcus aureus, salmonella typhimurium and escherichia coli; the bacillus altitudinis MLF-1 has good fermentation capacity on the siraitia grosvenorii residues, after the bacillus altitudinis MLF-1 is mixed with the bacillus licheniformis strain JSF-9 and the bacillus safensis strain MLL-5 to prepare the complex microbial inoculant, the nutritional value and the disease resistance of the siraitia grosvenorii residues fermented by the complex microbial inoculant are remarkably improved, 10%-15% of the complex microbial inoculant is added into a basic ration, and the content of the complex microbial inoculant in the siraitia grosvenorii residues fermented by the complex microbial inoculant is reduced. The Jersey cattle feed has the effects of reducing cost and improving efficiency for feeding Jersey cattle.

The present invention discloses the use of 2-aminoquinoline in the preparation of a drug for inhibiting Salmonella typhimurium. The present invention inhibits the expression of QS-related genes and virulence genes and the formation of biofilms by binding 2-aminoquinoline to an LsrB protein, thereby reducing the pathogenicity of Salmonella typhimurium. 2-aminoquinoline can also inhibit the invasion and adhesion capabilities of Salmonella typhimurium to cells. Therefore, 2-aminoquinoline can be used in the preparation of a drug for inhibiting Salmonella typhimurium.

The invention relates to the technical field of poultry immunology, in particular to a universal riemerella anatipestifer monoclonal antibody, a detection kit and application of the universal riemerella anatipestifer monoclonal antibody. Specifically, the invention successfully designs the universal riemerella anatipestifer monoclonal antibody, and the detection kit developed based on the monoclonal antibody can rapidly detect the level of the riemerella anatipestifer serum antibody in a sample, and has high sensitivity and strong specificity. The riemerella anatipestifer strain has no cross reactivity with avian enterococcus faecalis, avian salmonella typhimurium, avian escherichia coli and avian salmonella enteritidis, so that the riemerella anatipestifer strain can be applied to rapid screening, epidemiological monitoring and vaccine immune effect evaluation of riemerella anatipestifer infection, and has a wide application prospect.

Disclosed is a method and a device for determining whether a sample contains ampicillin-resistant (AMP-R) non-typhoid Salmonella (NTS). The method comprises detecting, in the sample, the presence of a combination of certain genes or their gene products, wherein the presence of said combination indicates the sample contains AMP-R NTS.