44 results about "Eubacterium" patented technology

The invention relates to an eubacterium ventriosum and two-shape eubacterium preparation and application thereof, in particular to microbial ecological preparation prepared by using eubacterium ventriosum or / and two-shape eubacterium as main active ingredients, and application of the microbial ecological preparation for treating relative diseases. The microbial ecological preparation comprises medicine composition, health products, drinks, veterinary drug and feed additive.

The invention relates to a eubacterium and clostridium preparation and application of the same, in particular to a microecological preparation which is prepared for replenishing butyric acid bacteria and butyric acid produced by intestinal tract by taking single eubacterium, single clostridium or a eubacterium and clostridium composition as a main active composition, and application of the same in treating related diseases through butyric acid production, and belongs to the field of biological medicine.

A dietary supplement that may contain probiotics from the genera Akkermansia, Bacteriodes, Faecalibacterium, Eubacterium, Escherichia, Collinsella, Desulfovibrio, Clostridium, Mycobacterium, Pediococcus, and Bifidobacterium. The dietary supplement may provide a variety of benefits including weight management, blood sugar management, treatment of irritable bowel syndrome, treatment of Crohn's disease, treatment of diverticulitis, treatment for inflammatory bowel, treatment for dysbiosis, and strengthening the immune system.

Biomarkers for diabetes and usages thereof are provided. And the biomarkers are Akkermansia muciniphilacBacteroides intestinaliscBacteroides sp. 20_3cClostridium bolteaec Clostridium hathewayicClostridium ramosumcClostridium sp. HGF2cClostridium symbiosumc5 Desulfovibrio sp. 3_1_syn3cEggerthella lentacEscherichia colicClostridiales sp. SS3 / 4c Eubacterium rectalecFaecalibacterium prausnitziicHaemophilus parainfluenzaecRoseburia intestinalis and Roseburia inulinivorans.

The present invention discloses an oral composition comprising a combination of probiotic bacteria selected from the group comprising Streptococcus, Eubacterium, Neisseria, Veillonella and pH-rising and / or buffering components, which re-establishes an oral microflora associated with good oral health in subjects with a disturbed oral microflora. The invention also discloses the use of the oral composition in combination with other oral health promoting products to help subjects suffering from disorders such as oral dryness, caries, halitosis, inflamed oral mucous membranes or oral fungal infections to restore good oral health.

The present invention relates to a biomarker for risk prediction or diagnosis of metabolic and inflammatory diseases, and use thereof. The biomarker is formulated to detect a group of microorganisms consisting of Akkermansia muciniphila, Bacteroides spp., Eubacterium spp., Roseburia spp., Methanobrevibacter smithii, Methanosphaera stadtmanae, Ruminococcus obeum, and Phascolarctobacterium.

A method of expressing a protein having an unnatural amino acid integrated thereinto characterized by comprising expressing (A) a mutant tyrosyl tRNA synthase which is a mutant of Escherichia coli-origin tyrosyl tRNA synthase and has an elevated specificity for a unnatural tyrosine derivative compared with the specificity for tyrosine, (B) a suppressor tRNA originating in an eubacterium belonging to the genus Bacillus, Mycoplasma or Staphylococcus which is capable of binding to the above tyrosine derivative in the presence of the above mutant TyrRS, and (C) a desired protein gene having a nonsense mutation at a desired site in animal cells to thereby incorporate the above tyrosine derivative into the nonsense mutation site in the above protein.

Microorganism of the genus Eubacterium, and its obtainment and use, which is suitable in pure culture, DSM 11798, and / or mixed culture with the strain Enterococcus casseliflavus, DSM 11799, or in mixed culture with other anaerobic microorganisms for the detoxification of trichothecenes.A feedstuff additive for the inactivation of trichothecenes in feedstuffs or in the digestive tract of animals contains a pure and / or mixed culture of the microorganism (DSM 11798 or DSM 11799) or a mixed culture with other anaerobic microorganisms in an amount from 0.2 to 3 kg, in particular 0.5 to 2.5 kg, per 1000 kg of feedstuff. The feedstuff additive containing DSM 11798 achieves probiotic effect on an animal and maintains or improves fertility performance of an animal subject to fusariotoxin-contaminated feed.

A method of expressing a protein having an unnatural amino acid integrated thereinto comprising expressing (A) a mutant tyrosyl tRNA synthase that is derived from Escherichia coli-origin tyrosyl tRNA synthase and has an elevated specificity for an unnatural tyrosine derivative compared with the specificity for tyrosine, (B) a suppressor tRNA originating in an eubacterium belonging to the genus Bacillus, Mycoplasma or Staphylococcus which is capable of binding to the above tyrosine derivative in the presence of the above mutant TyrRS, and (C) a desired protein gene having a nonsense mutation at a desired site in animal cells to thereby incorporate the above tyrosine derivative into the nonsense mutation site in the above protein.

The present disclosure describes compositions and methods for increasing the abundance of commensal bacteria belonging to the order Clostridiales, including Blautia, Ruminococcus, Clostridium, Eubacterium, Holdemania and Dorea species, that are as-sociated with reduced lethal GVHD and improved overall survival following bone mar-row or hematopoietic stem cell transplant. The present disclosure, therefore, provides methods for reducing the likelihood, incidence or severity of GVHD by (1) avoiding the loss of endogenous beneficial species through antibiotic selection; (2) by administering a therapeutically effective amount of a composition comprising one or more Clostridiales associated with reduced GVHD to individuals who may lack or have lost those strains from their intestinal microbiota. Additionally, support for endogenous or reestablished Clostridiales related to reduced GVHD as a treatment option for reducing GVHD can also be provided in the form of nutritional supplementation, for example, sugars fermented by some species of Clostridiales with GVHD reducing activity.

The invention provides a crystal form of (s)-ornidazole phosphate disodium, and a preparation method for the crystal form of the (s)-ornidazole phosphate disodium. In addition, pharmaceutical compositions containing the crystal form of the (s)-ornidazole phosphate disodium are applied in treatment of a plurality of infectious diseases caused by sensitive anaerobic bacteria like bacteroides fragilis, bacteroides distasonis, bacteroides ovatus, bacteroides thetaiotaomicron, bacteroides vulgatus, clostridium colinum, eubacterium, peptococcus and peptostreptococcus, helicobacter pylori, bacteroides melaninogenicus, fusobacterium, capnocytophaga and bacteroides gingivalis.

The present disclosure describes compositions and methods for increasing the abundance of commensal bacteria belonging to the order Clostridiales, including Blautia, Ruminococcus, Clostridium, Eubacterium, Holdemania and Dorea species, that are associated with reduced lethal GVHD and improved overall survival following bone marrow or hematopoietic stem cell transplant. The present disclosure, therefore, provides methods for reducing the likelihood, incidence or severity of GVHD by (1) avoiding the loss of endogenous beneficial species through antibiotic selection; (2) by administering a therapeutically effective amount of a composition comprising one or more Clostridiales associated with reduced GVHD to individuals who may lack or have lost those strains from their intestinal microbiota. Additionally, support for endogenous or reestablished Clostridiales related to reduced GVHD as a treatment option for reducing GVHD can also be provided in the form of nutritional supplementation, for example, sugars fermented by some species of Clostridiales with GVHD reducing activity.

The invention brings forward biomarkers for end-stage renal disease and an application thereof. The biomarkers for end-stage renal disease are Akkermansia muciniphila, Alistipes finegoldii, Alistipesshahii, Bacteroides fibrobacter, Bacteroides fragilis, Bifidobacterium dentium, Clostridium difficile, Clostridium saccharolyticum, Desulfovibrio Vulgaris, Eggerthella lenta, Enterococcus faecalis, Enterococcus faecium, Flavonifractor plautii, Fusobacterium nucleatum, butyric acid-producing enterobacteriaceae, Lactobacillus amylovorus, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus plantarum, Streptococcus infantarius, Streptococcus thermophilus, butyrate-producing bacteria, Eubacterium rectal and Faecalibacterium prausnitzi. Whether a subject suffers from or is susceptible to end-stage renal disease can be effectively determined by determining these microbial markers in the intestinal flora of the subject.

The invention discloses a multi-fluorescent PCR (polymerase chain reaction) detection kit for periodontitis pathogenic bacteria. The multi-fluorescent PCR detection kit has the advantages that 10 types of main periodontitis pathogenic bacteria including actinobacillus actinomycetemcomitan, porphyromonas gingivalis, prevotella intermedia, treponema denticola, tannerella forsythia, fusobacterium nucleatum, campylobacter gracilis, campylobacter rectus, entanglement eubacterium and streptococcus constellatus can be detected by the multi-fluorescent PCR detection kit, the quality can be controlled in integral test procedures for acquiring samples, extracting nucleic acid, carrying out PCR amplification and the like, and detection results can be quickly obtained; the multi-fluorescent PCR detection kit for the periodontitis pathogenic bacteria can be used for quickly detecting the main periodontitis pathogenic bacteria.

The invention provides a technology for preparing an antibacterial peptide. The technology comprises the following steps of mixing 2-4 parts by weight of bacillus licheniformis, 0.5-1.5 parts by weight of bacillus cereus and 1-2 parts by weight of bacillus natto to obtain a uniform mixture, carrying out mixture fermentation at a temperature of 36-39 DEG C for 6-10h to obtain storage strains, carrying out storage strain fermentation in a fermentation tank at a temperature of 36-39 DEG C for 27-37h, carrying out plate-frame pressure filtration on the fermented storage strains, and carrying out spray drying on the filtered storage strains to obtain the antibacterial peptide. The triple strain fermentation product obtained by the technology has effects on hemolytic streptococcocci, pneumococcocci, enterococcus, corynebacterium diphtheria, bacillus anthracis, gram-positive anoxic bacillus, achalme bacillus, clostridium tetani, clostridium difficile, eubacterium and lactic acid bacillus. The technology can shorten a production period by 1 / 3 and reduce a use cost by 60%.

The invention provides a preparation method of a ruminant fermented feed. The preparation method comprises the following steps of: 1) mixing two to six of six feed raw materials including corn bran, corn germ meal, corn sugar residues, sea buckthorn seed meal, palm kernel meal and xanthoceras sorbifolia meal according to a certain ratio to obtain a first mixture; 2) preparing a liquid fermentation culture medium and sterilizing at high temperature and high pressure; 3) inoculating ruminococcus, eubacterium and amylophilus ruminococcus into three parts of the liquid fermentation culture medium respectively to prepare corresponding seed solutions; and 4) adding bacillus licheniformis, bacillus subtilis, lactobacillus plantarum, saccharomyces cerevisiae, mannase, xylanase, acid protease and acid cellulase into any one seed solution prepared by step 3); after uniformly mixing, adding a mixture into the first mixture and fermenting; and after fermenting, obtaining the fermented feed. According to the preparation method provided by the invention, corn byproducts and miscellaneous meal are used as the raw materials and are subjected to biological pre-treatment to prepare the ruminant feed, so that the additional value of the corn byproducts is improved.

A red dragon fruit enzyme, preparation method and application thereof relate to the field of health food with health care effects. The red pitaya enzyme in the embodiment of the present invention is mainly prepared from the red pitaya fermentation liquid rich in short-chain fatty acids and functional galacto-oligosaccharides. The microbial combination starter of Fidobacterium, Bacteroides, and Eubacterium is obtained by fermentation and filtration by a three-step directional fermentation method. The pitaya enzyme is easily absorbed by the human body, has comprehensive effects, and has high economic value. The pitaya enzyme of the embodiment of the present invention can be used to regulate the gene expression of gastrointestinal mucosal cells, repair gastrointestinal mucosa, and assist in the treatment of gastric ulcer; it can be used to regulate the intestinal flora environment, relieve bowel movements, and prevent colorectal cancer; Improve the basic metabolism of the human body and have a good hypoglycemic effect.

The invention discloses a use method of pharmaceutical composition for preventing and treating powdery mildew. The use method includes the following steps that A. a stock solution is prepared, and components of the stock solution are 30% difenoconazole, 80% thiophanate methyl wettable powder, mint, cassia twig, baicalein, 75% chlorothalonil, gypsum and fructus anidii; B. water is added into the stock solution to be mixed to prepare diluent of 400-600 mass multiple ratio; C. 3.5-4kg of the diluent is used for each mu, spraying is conducted evenly on the front and the back of each leaf, especially on sick leaves, and spraying protection is conducted on upper healthy leaves; D. fertilizer is supplemented 3 days after the spraying is conducted, fertilizing loss and premature senility are prevented, plant disease resistance is improved, watering is not required in cloudy days after fertilizer is applied, ventilation is often conducted in sunny days, relative humidity of a greenhouse is reduced, temperature is prevented from being too high, and stuffiness is prevented; E. 3 days after spraying, sulphur smudging is conducted, and smudging is conducted for 10 minutes every day in the greenhouse. The use method is easy in matching, convenient to use and good in cucumber mildew treating effect. Through the tests, diluent of mixture of 400-600 mass multiple ratio has a remarkable inactivation effect on eubacterium fungi imperfecti sphaerotheca fuliginea powdery mildew and is good in sterilizing effect.

The invention discloses a drug resistant to pseudomonas solanacearum. The drug comprises the following components in part by weight: 50% germicide wettable powder 10 to 15 parts, 12% copper abietate emulsion 8 to 12 parts, streptomycin sulfate wettable powder 10 to 15 parts, cinnamyl aldehyde 4 to 8 parts, matrine 4 to 8 parts, polyoxin 6 to 10 parts, radix bupleuri 4 to 8 parts, and coriander 6 to 10 parts. The drug provided by the invention has the advantages of simple recipe, high convenience in use, and good effect in treating potato bacterial wilt. Tests show that the 400 to 600 times solution of the mixture has obvious effect in inactivating sphaerotheca fuliginea of the deuteromycotina of eubacterium, and has good sterilization effect.

The invention discloses a disease-resistant composition for bacterial wilt. The disease-resistant composition comprises, by weight, 10-15 parts of potassium sulfate, 8-12 parts of 15% cuaminosulfate aqueous solutions, 10-15 parts of 5% chitosan oligosaccharide solutions, 4-8 parts of sophocarpidine, 4-8 parts of lignin, 6-10 parts of polyoxybiotic solvent, 4-8 parts of plant ash aqueous extract and 6-10 parts of pericarpium citri reticulatae. The disease-resistant composition is simple in proportion, convenient to use and good in treating effect for the potato bacterial wilt; through experiments and tests, 400-600 times solutions of the composition have the significant inactivation effect on eubacterium imperfect fungi subdivision sphaerotheca powdery mildew and are good in sterilizing effect.

The invention discloses a pesticide for preventing and controlling cladosporium cucumerinum. The pesticide comprises the following components in parts by weight: 10-15 parts of 50 percent carbendazim, 8-12 parts of 70 percent thiophanate methyl, 10-15 parts of new high-fat film, 4-8 parts of vegetable and fruit pedicel strengthening agent, 4-8 parts of astragalus polysaccharide, 6-10 parts of glycyrrhizic acid, 4-8 parts of isatis root and 6-10 parts of fructus forsythia. The pesticide has a simple proportion, is convenient to use and has a good treatment effect on the leaf mold; as tested, the 400-600-times solution of the mixture has an obvious deactivation effect and a good sterilization effect on eubacterium deuteromycotina and eubacterium cladosporium fulvum.

This invention relates, e.g., to methods for detecting a aubacterium, determining if the eubacterium is Gram-positive or Gram-negative, and determining the species of the eubacterium in a sample.

The present invention relates to novel nucleic acid sequences encoding bacterial xylose isomerases that upon transformation of a eukaryotic microbial host cell, such as yeast, to confer to the host cell the ability of isomerising xylose to xylulose. The nucleic acid sequences encode xylose isomerases that originate from bacteria such as Eubacterium sp., Clostridium cellulosi and others. The invention further relates to fermentation processes wherein the transformed host cells ferment a xylose-containing medium to produce ethanol or other fermentation products.

The invention discloses a Eubacterium limosum and its application. The Eubacterium limosum is Eubacterium limosum ZL-II concretely, and has a preservation number of CGMCC No.6846 in China General Microbiological Culture Collection Center, and the application of the Eubacterium limosum is the application of the Eubacterium limosum ZL-II with the preservation number of CGMCC No.6846 in enterodiol production concretely. The Eubacterium limosum ZL-II with the preservation number of CGMCC No.6846 plays a great role in the preparation of a phytoestrogen component enterodiol by biologically converting a natural product. An enterodiol production technology provided by the invention has the advantages of simplicity, low production cost, no environmental pollution, suitableness for large scale production, and important economic benefit.

The invention belongs to the field of biological agents, and particularly relates to a biological agent for preventing yak gastrointestinal diseases and a preparation method. The technical scheme particularly includes that the biological agent for preventing the yak gastrointestinal diseases comprises, by viable bacteria quantity, bacillus natto, Bacillus bulgaricus, clostridium butyricum, Lachnospira multiparus and non-sucroclastic Eubacterium with the ratio of 1:(2-5):(5-10):(3-5):(3-5). The viable bacteria quantity of the biological agent is larger than or equal to 6*10<8>CFU / ml. The invention further provides the particular preparation method of the biological agent. By the aid of the biological agent, young yaks can be helped to rapidly build gastrointestinal probiotic groups, the biological agent can serve as daily intestinal healthcare food for adult yaks and can also be used as adjuvant drugs for diseased yaks, and the diseased yaks are helped to rapidly recover.

The invention discloses a composition for resisting cladosporium fulvum. The composition is composed of, by weight, 10-15 parts of 35% carbendazim, 8-12 parts of 22% thiophanate methyl, 10-15 parts of lignin, 4-8 parts of monopotassium phosphate, 4-8 parts of 3% chitosan solution, 6-10 parts of glycyrrhizic acid, 4-8 parts of radix isatidisand and 6-10 parts of fistular onion stalk juice. The composition is simple in ratio, convenient to use and good in cladosporium fulvum resisting effect; through experiments and tests, 400-fold to 600-fold solutions of the mixture have a remarkable inactivating effect on the eubacterium deuteromycotina cladosporium fulvum and are good in sterilizing effect.

The present invention provides a method, wherein the method treats a subject having a dysbiosis, the method comprising: determining a first metabolic profile of the gut microbiome of a subject having a dysbiosis; changing the first metabolic profile of the gut microbiome of the subject to a second metabolic profile of the gut microbiome of the subject, by administering to the subject a composition comprising at least one bacterial species selected from the group consisting of: Acidaminococcus intestinalis, Bacteriodes ovatus, Bifidobacterium adolescentis, Bifidobacterium longum, Blautia sp., Clostridium sp., Collinsella aerofaciens, Escherichia coli, Eubacterium desmolans, Eubacterium eligens, Eubacterium limosum, Faecallbacterum prausnitzii, Lachnospira pectinoshiza, Lactobacillus casei, Parabacteroides distasonis, Roseburia faecalis, Roseburia intestinalis, Ruminococcus sp., Ruminococcus species, and Ruminococcus torques, wherein the composition is administered at a therapeutically effective amount, sufficient to alter the first metabolic profile of the gut microbiome to the second metabolic profile of the gut microbiome.

The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of the positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymererase beta-sub-unit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.