40results about How to "Short enrichment time" patented technology

The invention provides PD-L1 antibody immunomagnetic beads and a preparation method thereof. Each PD-L1 antibody immunomagnetic bead comprises a magnetic microsphere part and an antibody part; the immunomagnetic beads respectively have a structural formula of A-NH-N=CH-B, wherein A represents a magnetic microsphere and B represents a PD-L1 antibody, or A represents a PD-L1 antibody and B represents a magnetic microsphere. When being used for capturing tumor cells, the immunomagnetic beads are not only good in specificity and sensitivity, but also rapid in magnetic response, short in enrichment time and high in capture efficiency. Furthermore, the immunomagnetic beads are stable in properties, small in particle size, good in magnetic responsiveness and good in dispersity. The preparation method is simple and has very high practicality.

The invention relates to a full-automatic sample thermal desorption and liquid phase micro-enriching device which comprises a heating device, a condensing device, an enriching device and a controller, wherein the heating device comprises a heating body; a heating slot is formed in the heating body; a quartz sample pipe is arranged in the heating slot; a high-temperature polytetrafluoroethylene pad is arranged at a pipe opening of the quartz sample pipe; a stainless steel pipe is arranged between the heating body and the quartz sample pipe; one end of the stainless steel pipe is arranged in the quartz sample pipe; the other end of the stainless steel pipe is connected with a micro-flow controller; a nitrogen cylinder is connected with the micro-flow controller; a syringe needle is arranged on the high-temperature polytetrafluoroethylene pad; one end of the syringe needle is arranged in the quartz sample pipe; the other end of the syringe needle is connected with the condensing device; the enriching device is connected with the condensing device; electric connecting wires are arranged between the heating body and the micro-flow controller as well as the controller. The full-automatic sample thermal desorption and liquid phase micro-enriching device has the advantages of simple structure, convenience in use, low cost, integrated function of extracting, purifying and condensing, less dosage, short enriching time and higher enriching efficiency.

The invention relates to an electrochemical measuring method of ferric iron content, and an electrochemical three-electrode testing system and a constant-current anode stripping differential timing potentiometric method are adopted to carry out the measurement. The electrochemical measuring method is characterized by comprising the following steps: reducing ferric iron by hydroxylamine hydrochloride, extracting chelate Fe-[(C12H8N2)3]<2+> formed by the chelation of 1,10-orthophenanthrolene into propylene carbonate organic solvent and enriching on the surface of a working electrode, obtaining adT/dE-E differential curve by recording a function of the change of an electric potential E on the working electrode along with the testing time T and carrying out the differential processing on thefunction, and calculating the ferric iron content according to the height dT/dE (sV<-1>) of an iron response peak that is generated when an anode stripping electric potential on the differential curveis plus 400mV to plus 500mV. The invention has simple process, convenient operation, high sensitivity and low operation cost and can carry out analysis and testing on micro or trace ferric iron in natural water quality.

The invention relates to a method for detecting CTCs by multi-antibody immunomagnetic bead enrichment. The method comprises specific steps as follows: 1, coupling an anti-EpCAM monoclonal antibody with magnetic beads; 2 preparing a circulating tumor cell sample: adding a proper quantity of immunomagnetic beads to a biological tumor cell sample, performing uniform mixing, then, performing separation by a magnetic separator and PBS cleaning, suspending magnetic bead adsorbed cells in a PBS for cell counting, and detecting enriched cells by a cell counting plate; adding a proper quantity of nanoimmunomagnetic beads to an artificial tumor cell blood sample for magnetic separation, performing PBS cleaning, and then, suspending magnetic bead adsorbed cells in the PBS again; taking the sample, dropwise adding the sample to a glass slide, performing incubation by FICT, detecting enriched cells by a flow cytometer, and identifying effectiveness by tumor cell-fluorescence labeling EPCAM. Counting is performed under a fluorescence inverted microscope, and enrichment and identification of tumor cell line cells mixed with peripheral blood are the same as above. The sensitivity and specificityof circulating tumor cell detection are greatly improved.

The invention discloses a salmonella bacteriophage nano magnetic bead conjugate and its enrichment and separation kit. The conjugate is a complex of a salmonella bacteriophage coupled with a carboxylmagnetic bead, wherein the salmonella bacteriophage is Salmonella Typhimurium bacteriophage LPST10, and a preservation number is CCTCC NO: M 2016473. The bacteriophage of the Salmonella bacteriophagenano magnetic bead conjugate of the invention can specifically recognize and infect bacteria, and has the characteristics of good specificity and small molecular weight. The kit is capable of rapidlyand sensitively separating and enriching salmonella.

The invention relates to a method for enriching heavy metals by using a water plant, which comprises the step of enriching the heavy metals from water bodies by using the water plant. the method is characterized in that the water plant is a plant species named as hydrilla verticillata. The concrete enriching method comprises the following steps: exposing hydrilla verticillata in certain weight to a heavy metal ionic solution with a pH value of 3-7 and the concentration of 1-200mg/L, reconfiguring the solution every 24 hours, and continuously exposing for 1-50 days. The method has the advantages that the super-enriching water plant of heavy metal copper and lead has a good enriching effect on heavy metal copper and lead in water, has good tolerance to the copper and lead, and has short enriching time. The method can be used for treating sewage, and particularly for removing the heavy metals in the sewage.

The invention relates to a low-temperature-resistant methanogen enrichment culture method and an application thereof. The low-temperature-resistant methanogen enrichment culture method comprises the following steps: firstly, taking activated sludge, breaking into pieces, inoculating into an enrichment culture medium, and determining enrichment culture temperature at the temperature of 15-25 DEG C according to sources of inocula; and secondly, when bubbles are detected in a culture system, gradually lowering temperature by virtue of a temperature control system, and stopping temperature lowering until no bubble is detected, so that low-temperature-resistant methanogen enrichment culture is completed. An application mode comprises that enrichment culture products are added into an accelerator prepared from ferric chloride, cobalt chloride, nickel chloride and manganese chloride and the obtained mixture is directly applied to low temperature methane fermentation. The low-temperature-resistant methanogen enrichment culture method can be used for solving the problems that preparation steps of an existing low temperature methanogen agent are tedious, low temperature domestication time is long and status of dominant bacteria is difficultly maintained for a long time at a low temperature. The low-temperature-resistant methanogen enrichment culture method is simple and easy to operate, has the advantages of short enrichment time, stable performance, good and stable running effect, available flora source and the like and is convenient for popularization and application.

The invention provides an E-cadherin, Cadherin-11 and EpCAM multi-antibody immunomagnetic bead and a preparation method thereof. The multi-antibody immunomagnetic bead comprises E-cadherin immunomagnetic beads, Cadherin-11 immunomagnetic beads and EpCAM immunomagnetic beads, wherein the E-cadherin immunomagnetic beads, the Cadherin-11 immunomagnetic beads and the EpCAM immunomagnetic beads are immunomagnetic beads respectively obtained by coupling an E-cadherin antibody, a Cadherin-11 antibody and an EpCAM antibody with magnetic microspheres. The multi-antibody immunomagnetic bead is used for capturing CTC and has the effects that the specificity and the sensibility are good, the magnetic response is rapid, the enrichment time is short, and the capturing efficiency is high; and furthermore, the performance is stable, the particle size is small, and the magnetic responsiveness and the dispersity are good. Furthermore, the preparation method is simple and has very strong practicability.

The invention provides a method for rapidly detecting pathogenic bacteria at high efficiency, a biological dependent sensor and a preparation method thereof, wherein the biological dependent sensor comprises a plastid with the function of converting signals and a capturing system with the function of recognizing molecules; the method for rapidly detecting the pathogenic bacteria at high efficiency comprises the following steps: preparing the biological dependent sensor; using the biological dependent sensor to capture a sample detecting solution; simultaneously setting the blank comparison and the positive concentration comparison of a standard substance and starting anadenosine triphosphate synthetic reaction; and carrying out the detection by utilizing a fluorescence scanner. The schemein the invention has the advantages of low detection limit and short detection time; the detection limit can reach 100cfu/hole; if the detection limit is computed according to 1cfu/25g, the whole detecting course is not beyond 12 hours, is shortened by at least 60 hours than the conventional microorganism test method and is shortened by at least 36 hours than a PCR (Polymerase chain Reaction) method; and when the concentration of the bacteria in a sample is higher than 1cfu/25g, the needed time is short, thus the deep influence on food safety and disease treatment bacterium control can be generated.

The invention discloses a method for electrochemical enrichment culture of an anaerobic ammonia oxidation (Anammox) biomembrane. The method adopts a biological electrochemical system technology, and comprises the following steps: firstly, using acetic acid as an electron donor to form a stable biomembrane; then changing the electron donor from the acetic acid into an ammonium salt as the only electron donor; and performing acclimation for a short time to achieve enrichment culture of a biomembrane with an anaerobic ammonia oxidation function. The biomembrane can transform ammonia nitrogen in sewage into a gaseous nitrogen product under an anaerobic condition to achieve one-step rapid removal of nitrogen and generate current at the same time. The method for the electrochemical enrichment culture of the anaerobic ammonia oxidation biomembrane, disclosed by the invention, is simple, and has a short enrichment time (45 days for the best) and a short start time (15 days for the best), and the formed anaerobic ammonia oxidation biomembrane is stable, has a good denitrification effect, and can be widely applied to a sewage denitrification process.

The invention discloses an electrophoresis microchip with an online ion enrichment device. The electrophoresis microchip comprises an electrophoresis microchip body and a sample injection pool arranged in the electrophoresis microchip body. The sample injection pool is communicated with a sample enrichment pool through ion enrichment flow channels. The sample enrichment pool is communicated with asample waste liquid pool through an ion sample injection flow channel. A buffer solution injection tank and a buffer solution waste tank are communicated through an ion separation flow channel. The ion sample injection flow channel and the ion separation flow channel are vertically arranged in a crossed manner and are internally communicated. The volume of the sample injection pool is larger thanthat of the sample enrichment pool, and the ion enrichment flow channels are located at the shortest distance between the sample injection pool and the sample enrichment pool. The invention also discloses a detection method using the electrophoresis microchip. A two-time enrichment structure and a horn mouth shape are innovatively adopted, the ion detection sensitivity of the electrophoresis microchip is greatly improved, and ions can be rapidly enriched, separated and detected on the electrophoresis microchip.

The invention relates to a GS-Au/AuNPs/g-C3N4 composite material, an electrochemical sensor and the application of the GS-Au/AuNPs/g-C3N4 composite material and the electrochemical sensor in the detection of mercury ions in a water body. The composite material is prepared by the following methods of dispersing the carboxyl modified g-C3N4 powder into methanol, then adding glutathione and a chloroauric acid aqueous solution, carrying out ultrasonic treatment, carrying out heating reflux treatment for 12-24 hours after the ultrasonic treatment, separating, washing and drying to obtain GS-Au/AuNPs/g-C3N4. The electrochemical sensor comprises a substrate electrode and the GS-Au/AuNPs/g-C3N4 composite material, and the GS-Au/AuNPs/g-C3N4 composite material is attached to the substrate electrode. The electrochemical sensor prepared from the GS-Au/AuNPs/g-C3N4 composite material can detect the concentration of Hg ions in the water body with high sensitivity and high specificity.

The invention provides HER2 antibody immunomagnetic bead and a preparation method of the HER2 antibody immunomagnetic bead. The HER2 antibody immunomagnetic bead comprises a magnetic microsphere part and an antibody part, wherein the structural formula of the immunomagnetic bead is A-NH-N=CH-B, A refers to a magnetic microsphere, B refers to an HER2 antibody, or A refers to the HER2 antibody and B refers to the magnetic microsphere. The immunomagnetic bead disclosed by the invention is not only good in specificity and sensitivity, but also rapid in magnetic response, short in enrichment time and high in capture efficiency while used for capturing tumor cells; furthermore, the immunomagnetic bead is stable in properties, and further small in particle size, good in magnetic response and excellent in dispersion. Moreover, the immunomagnetic bead has a simple preparation method and very strong practicability.

The invention discloses a method for separating and enriching sulfa antibiotics in drinking water. The method comprises a step of adjusting the pH of a water sample to 3 to 4 with acid and filtering to obtain a preprocessed water sample, a step of sequentially activating an MCX solid phase extraction column with methanol and ultrapure water and allowing sampled water to pass through the column, astep of drying the MCX solid phase extraction column under the protection of nitrogen after the enrichment is completed, rinsing the column with ultrapure water with pH of 3 to remove water-soluble interference impurities of the water sample enriched on the MCX solid phase extraction column, eluting with a methanol solution containing 8-15% ammonia water, slowly blowing an eluate with nitrogen stream to be nearly dry, and adding an aqueous solution re-dissolved residue containing methanol to complete the separation and enrichment of the sulfa antibiotics in the drinking water. The method of the invention has the advantages of simple operation, environmental friendliness and high enrichment ratio, multiple drugs of the same type of antibiotics and other drugs with similar physical and chemical properties to sulfonamides can be simultaneously enriched, the method is particularly suitable for the separation detection of trace sulfonamide antibiotics in a drinking water body, and the accuracy is high.

The invention relates to the technical field of sewage treatment, in particular to a domestic sewage treatment technology. The invention provides a domestic sewage treatment technology which comprises the steps of sequentially arranging an aerobic aeration tank, a secondary sedimentation tank, a concentration machine and a centrifugal machine, directly discharging sewage from the aerobic aeration tank to leave sludge, quickly adding a compound dephosphorization concentration agent, conveying to the concentration machine after uniform mixing, allowing the concentration machine to condense the sludge out of the aeration tank in a very short time, finally allowing the sludge to enter the centrifugal machine for dehydration, and discharging the sludge. The produced residual sludge can be directly concentrated and separated directly from the aeration tank; the concentration is short in time and high in speed; the problem of anaerobic phosphorus release caused by the long time of sludge concentration is solved; a content of phosphorus and COD (chemical oxygen demand) in clear water treated by the novel method is significantly reduced; the quality of the clear water is close to that of effluent of the secondary sedimentation tank; the water can be directly discharged or return to a treatment system for dilution; the system treatment capacity is improved.