40results about How to "Short enrichment time" patented technology

The invention provides a HER2, EGFR, EpCAM and MUC1 multiple antibody immunomagnetic bead and a preparation method thereof. The multiple antibody immunomagnetic bead includes a HER2 immunomagnetic bead, an EGFR immunomagnetic bead and an EpCAM immunomagnetic bead, and a HER2 antibody, an EGFR antibody and an EpCAM antibody are respectively coupled with magnetic microspheres to obtain the HER2 immunomagnetic bead, the EGFR immunomagnetic bead and the EpCAM immunomagnetic bead. The multiple antibody immunomagnetic bead is good in specificity and sensitivity, rapid in magnetic response, short in concentration time and high in capturing efficiency when being used for capturing CTCs (circulating tumor cells). The immunomagnetic bead is stable in nature, small in particle size, good in magnetic response and dispersibility, simple in preparation method and high in practicability.

The invention provides HER2 antibody immunomagnetic bead and a preparation method of the HER2 antibody immunomagnetic bead. The HER2 antibody immunomagnetic bead comprises a magnetic microsphere part and an antibody part, wherein the structural formula of the immunomagnetic bead is A-NH-N=CH-B, A refers to a magnetic microsphere, B refers to an HER2 antibody, or A refers to the HER2 antibody and B refers to the magnetic microsphere. The immunomagnetic bead disclosed by the invention is not only good in specificity and sensitivity, but also rapid in magnetic response, short in enrichment time and high in capture efficiency while used for capturing tumor cells; furthermore, the immunomagnetic bead is stable in properties, and further small in particle size, good in magnetic response and excellent in dispersion. Moreover, the immunomagnetic bead has a simple preparation method and very strong practicability.

The invention discloses an electrochemical detection method for the selenium content. It uses the thermal cracking graphite as the electrode host; the bismuth formed by the BiCl3, Se(IV) in situ enrichment is as the working electrode, which forms a kind of compound with the enriching selenium. In the scanning electric potential of -1000mV--1200mV on cathode, it gets a sensitive catalyzing hydrogen wave detected by the Osteryang square wave cathode stripping volt-ampere method to detect the Se(IV). Because the Bi has no toxicity, the invention has avoided the traditional hydrargyrum electrode pollution. Also it has simple process, high sensitivity, low cost. It can analyze the trace selenium and its valency in natural water. So it has widely application future in industrial and agricultural production, environment protection, medical treatment and science study.

The invention provides CD133, CD24 and CD44 multiple antibody immunomagnetic beads. The multiple antibody immunomagnetic beads are composed of CD133 immunomagnetic beads, CD24 immunomagnetic beads and CD44 immunomagnetic beads, wherein the CD133 immunomagnetic beads are obtained by coupling CD133 antibodies and magnetic microspheres, the CD24 immunomagnetic beads are obtained by coupling CD24 antibodies and magnetic microspheres, and the CD44 immunomagnetic beads are obtained by coupling CD44 antibodies and magnetic microspheres. The multiple antibody immunomagnetic beads are used for capturing cancer stem cells and are good in specificity and sensitivity, rapid in magnetic response, short in enrichment time and high in capture efficiency. Moreover, the immunomagnetic beads are stable in property, small in particle size, good in magnetic responsiveness and dispersity and simple in preparation method and have high practicability.

The invention relates to a GS-Au / AuNPs / g-C3N4 composite material, an electrochemical sensor and the application of the GS-Au / AuNPs / g-C3N4 composite material and the electrochemical sensor in the detection of mercury ions in a water body. The composite material is prepared by the following methods of dispersing the carboxyl modified g-C3N4 powder into methanol, then adding glutathione and a chloroauric acid aqueous solution, carrying out ultrasonic treatment, carrying out heating reflux treatment for 12-24 hours after the ultrasonic treatment, separating, washing and drying to obtain GS-Au / AuNPs / g-C3N4. The electrochemical sensor comprises a substrate electrode and the GS-Au / AuNPs / g-C3N4 composite material, and the GS-Au / AuNPs / g-C3N4 composite material is attached to the substrate electrode. The electrochemical sensor prepared from the GS-Au / AuNPs / g-C3N4 composite material can detect the concentration of Hg ions in the water body with high sensitivity and high specificity.

The invention provides a CD20 antibody immunomagnetic bead which comprises a magnetic microsphere part and an antibody part. The structural formula of the immunomagnetic bead is A-NH-N=CH-B, wherein A represents a magnetic microsphere, B represents a CD20 antibody, or A represents the CD20 antibody, and B represents the magnetic microsphere. The immunomagnetic bead is used for capturing cancer cells, and is good in specificity and sensibility, fast in magnetic response, short in enrichment time and high in capturing efficiency. The immunomagnetic bead is stable in nature, meanwhile small in particle size, good in magnetic responsiveness and good in dispersibility. A preparation method is simple, and high practicality is achieved.

The invention provides a method for rapidly detecting pathogenic bacteria at high efficiency, a biological dependent sensor and a preparation method thereof, wherein the biological dependent sensor comprises a plastid with the function of converting signals and a capturing system with the function of recognizing molecules; the method for rapidly detecting the pathogenic bacteria at high efficiency comprises the following steps: preparing the biological dependent sensor; using the biological dependent sensor to capture a sample detecting solution; simultaneously setting the blank comparison and the positive concentration comparison of a standard substance and starting anadenosine triphosphate synthetic reaction; and carrying out the detection by utilizing a fluorescence scanner. The schemein the invention has the advantages of low detection limit and short detection time; the detection limit can reach 100cfu / hole; if the detection limit is computed according to 1cfu / 25g, the whole detecting course is not beyond 12 hours, is shortened by at least 60 hours than the conventional microorganism test method and is shortened by at least 36 hours than a PCR (Polymerase chain Reaction) method; and when the concentration of the bacteria in a sample is higher than 1cfu / 25g, the needed time is short, thus the deep influence on food safety and disease treatment bacterium control can be generated.

The invention discloses a method for quickly improving the enrichment rate and degree of ammonia-oxidizing bacteria (AOB) in a sequencing batch reactor (SBR). The method is characterized by adopting an activated sludge process to treat ammonia-nitrogen wastewater in the SBR, using the mobile slope (MSC) of the oxidation-reduction potential (ORP) as an indication parameter of the ammoxidation process and increasing the enrichment rate of the AOB, improving the enrichment degree of the AOB by firstly improving the ammonia-nitrogen concentrations of feedwater gradually to culture the activities of nitrifying bacteria and then utilizing high free ammonia (50-65mg NH3 / L), high free nitrite acid (2-8mgHNO2-N / L) and proper amount of discharged sludge to elutriate nitrite nitrogen oxidizing bacteria and heterotrophic bacteria out of the system. The enrichment rate and degree of the AOB in the SBR are quickly improved by adopting the method. An AOB enrichment reactor can increase the proportion of the AOB in the total bacteria to 11% from 3% in about 40 days and improve the proportion to 90% in about 100 days. The sludge load can reach 1.31kgNH4<+>-N / (gvss.d). The method has the advantages of short AOB enrichment time, high AOB enrichment degree, flexibility in control and the like.