A kind of electrochemical enrichment culture method of anammox biofilm

An anammox and bioelectrochemical technology, which is applied in the field of electrochemical enrichment culture of anammox biofilms, can solve the problems of long start-up time, difficult cultivation, slow growth of anammox bacteria, etc. Fast time, stable anammox biofilm, good denitrification effect

Active Publication Date: 2020-02-14
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
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Problems solved by technology

[0006] The application of anammox technology has good development prospects and advantages, but it has not been widely used in biological denitrification engineering practice, mainly because the growth of anammox bacteria is slow and the cultivation is difficult, which leads to the start-up time of the whole process. Very long; at the same time, how to maintain a high concentration of biomass is the key to efficient denitrification of sewage

Method used

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  • A kind of electrochemical enrichment culture method of anammox biofilm
  • A kind of electrochemical enrichment culture method of anammox biofilm
  • A kind of electrochemical enrichment culture method of anammox biofilm

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Embodiment 1

[0042] The electrochemical enrichment culture method of embodiment 1 anammox biofilm

[0043] (1) Construction of bioelectrochemical reactor

[0044] The shell of the bioelectrochemical reactor is cuboid plexiglass, and the interior is a cylindrical structure with a length of 6cm and a diameter of 3cm. The working electrode is a carbon brush with a length of 3cm and a diameter of 3cm, and the auxiliary electrode is a carbon cloth with a diameter of 3cm. The bioelectrochemical reactor and The CHI1040c multi-channel electrochemical workstation of Shanghai Chenhua Company is connected, and the anode potential is set to 0V (the reference electrode is a saturated calomel electrode). The working schematic diagram of the bioelectrochemical reactor is as follows figure 1 shown.

[0045](2) Electrochemical enrichment culture of anammox biofilm

[0046] In the bioelectrochemical reactor, the inoculation amount of anaerobic activated sludge is 10% of the working volume, adding acetic a...

Embodiment 2

[0056] Example 2 Effect of anaerobic ammonium oxidation biofilm on ammonia nitrogen degradation

[0057] The anammox biofilm successfully enriched in Example 1 was inoculated in the ammonium chloride matrix culture solution containing 5 mM ammonium chloride, cultured at 30°C for 50 h, and tested for 0, 8, 18, 30, and 50 h, respectively. The content of nitrate nitrogen, nitrite nitrogen and ammonia nitrogen.

[0058] The result is as Figure 5 As shown, within 8 hours, the anaerobic ammonium oxidation reached 46% ± 2%; within 50 hours, 5mM ammonia nitrogen could be degraded by 80%, and the concentrations of nitrite and nitrate in the corresponding products were both low. It shows that the ammonia nitrogen in the matrix can be effectively oxidized by the anammox biofilm to gas products such as nitrogen or nitrogen oxides, so that the ammonia nitrogen can be removed from the sewage.

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Abstract

The invention discloses a method for electrochemical enrichment culture of an anaerobic ammonia oxidation (Anammox) biomembrane. The method adopts a biological electrochemical system technology, and comprises the following steps: firstly, using acetic acid as an electron donor to form a stable biomembrane; then changing the electron donor from the acetic acid into an ammonium salt as the only electron donor; and performing acclimation for a short time to achieve enrichment culture of a biomembrane with an anaerobic ammonia oxidation function. The biomembrane can transform ammonia nitrogen in sewage into a gaseous nitrogen product under an anaerobic condition to achieve one-step rapid removal of nitrogen and generate current at the same time. The method for the electrochemical enrichment culture of the anaerobic ammonia oxidation biomembrane, disclosed by the invention, is simple, and has a short enrichment time (45 days for the best) and a short start time (15 days for the best), and the formed anaerobic ammonia oxidation biomembrane is stable, has a good denitrification effect, and can be widely applied to a sewage denitrification process.

Description

technical field [0001] The invention belongs to the field of sewage treatment, and relates to an electrochemical enrichment and cultivation method of anaerobic ammonium oxidation biofilm. Background technique [0002] Anaerobic ammonium oxidation (Anammox) refers to the direct conversion of microorganisms to NH under anaerobic conditions. 4 ＋ As an electron donor, NO 2 － As an electron acceptor, the NH 4 ＋ and NO 2 － convert to N 2 biological oxidation process. In 1990, the Kluyver Biotechnology Laboratory of the Delft University of Technology in the Netherlands developed the Anammox process, which has been proposed for more than 20 years. Oxidation has many outstanding advantages: [0003] (1) There is no need to add organic matter as an electron donor, which can save costs and prevent secondary pollution; [0004] (2) Anaerobic ammonium oxidation can greatly reduce oxygen consumption and energy consumption; [0005] (3) The amount of biological acid produced by...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/28
CPCC02F3/28
Inventor 唐家桓黄玲艳陈姗姗周顺桂
Owner FUJIAN AGRI & FORESTRY UNIV
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