Electrophoresis microchip with online ion enrichment device and detection method

An ion enrichment and microchip technology, which can be used in measuring devices, chemical instruments and methods, and containers for laboratories, etc., can solve the problems of high cost, small enrichment ratio, and complicated operation.

Active Publication Date: 2019-12-17
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the isokinetic electrophoresis enrichment technology and sweeping technology, although the enrichment efficiency is high and the enrichment multiple is large, but the chip production is difficult, the cost is high, and the opera...

Method used

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  • Electrophoresis microchip with online ion enrichment device and detection method
  • Electrophoresis microchip with online ion enrichment device and detection method
  • Electrophoresis microchip with online ion enrichment device and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Such as Figure 1-6 As shown, an electrophoresis microchip with an ion online enrichment device includes an electrophoresis microchip body and a sample injection pool 7, a sample waste pool 9, a buffer injection pool 10, and a buffer solution disposed in the electrophoresis microchip body. Waste liquid pool 11, the sample injection pool 7 communicates with the sample enrichment pool 8 through the ion enrichment flow channel 4, and the sample enrichment pool 8 communicates with the sample waste liquid pool 9 through the ion sampling flow channel 5; The buffer injection pool 10 and the buffer waste pool 11 are communicated through the ion separation flow channel 6; the ion sampling flow channel 5 and the ion separation flow channel 6 are vertically intersected and communicated internally; the sample injection pool 7 The volume is larger than that of the sample enrichment pool 8 , and the ion enrichment channel 4 is located at the shortest distance between the sample injec...

Embodiment 2

[0067] A detection method using the above-mentioned electrophoresis microchip, comprising the following steps:

[0068] (1) Mix histidine / 2-morphineethanesulfonic acid, 18-crown-6 and deionized water to form high-concentration buffer B and low-concentration buffer A with a concentration ratio of 2:1;

[0069] Mix the sample to be tested with low-concentration buffer A at a volume ratio of 1:1 to make a sample solution;

[0070] (2) Add high-concentration buffer B into the sample waste liquid pool 9, buffer injection pool 10 and buffer waste liquid pool 11, and fill the ion sampling channel 5 and ion separation channel 6; Liquid A is added to the sample enrichment pool 8, and filled with the ion enrichment channel 4; the sample solution is added to the sample injection pool 7; it is completely filled; wherein the volume ratio of the sample injection pool 7 to the sample enrichment pool 8 is 12 :1;

[0071] (3) Apply an enrichment high voltage of 700V for 30s between the sample ...

Embodiment 3

[0076] A detection method using the above-mentioned electrophoresis microchip, comprising the following steps:

[0077] (1) Low-concentration buffer A is prepared by mixing 5mM / L histidine / 2-morphineethanesulfonic acid, 0.5mM / L 18-crown-6 and deionized water; it is composed of 20mM / L histidine / 2-Morphineethanesulfonic acid, 0.5mM / L 18-crown-6 and deionized water are mixed to form high-concentration buffer B, and the pH of low-concentration buffer A and high-concentration buffer B is 6.0;

[0078] The aqueous solution of KCl, NaCl, and LiCl (both analytical reagents) is used as the sample to be tested, and then the sample to be tested is mixed with low concentration buffer A at a volume ratio of 1:1 to make the sample solution with an ion concentration;

[0079] (2) Add high-concentration buffer B into the sample waste liquid pool 9, buffer injection pool 10 and buffer waste liquid pool 11, and fill the ion sampling channel 5 and ion separation channel 6; Liquid A is added in...

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Abstract

The invention discloses an electrophoresis microchip with an online ion enrichment device. The electrophoresis microchip comprises an electrophoresis microchip body and a sample injection pool arranged in the electrophoresis microchip body. The sample injection pool is communicated with a sample enrichment pool through ion enrichment flow channels. The sample enrichment pool is communicated with asample waste liquid pool through an ion sample injection flow channel. A buffer solution injection tank and a buffer solution waste tank are communicated through an ion separation flow channel. The ion sample injection flow channel and the ion separation flow channel are vertically arranged in a crossed manner and are internally communicated. The volume of the sample injection pool is larger thanthat of the sample enrichment pool, and the ion enrichment flow channels are located at the shortest distance between the sample injection pool and the sample enrichment pool. The invention also discloses a detection method using the electrophoresis microchip. A two-time enrichment structure and a horn mouth shape are innovatively adopted, the ion detection sensitivity of the electrophoresis microchip is greatly improved, and ions can be rapidly enriched, separated and detected on the electrophoresis microchip.

Description

technical field [0001] The invention relates to the field of microfluidic chip electrophoresis, in particular to an electrophoresis microchip with an ion online enrichment device and a detection method. [0002] technical background [0003] Ion detection technology plays a vital role and significance in many fields such as clinical medicine, water quality monitoring, soil survey, food and drug quality management. as K in soil + 、Na + , Li + , NH 4 + , Ca 2+ , Mg 2+ Plasma is an important indicator of soil quality survey; NH in water 4 + 、PO 4 + When the plasma content is too much, it will cause the eutrophication of the water body, the algae in the water body will multiply rapidly, and other water body organisms will die in large numbers, thus destroying the ecological balance; while the Cd in the water body and soil 2+ , Pb 2+ Such heavy metal ions will gradually accumulate in the food chain and eventually be brought into the human body to cause diseases. Due t...

Claims

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Application Information

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IPC IPC(8): G01N27/447B01L3/00
CPCB01L3/502707B01L3/50273B01L2400/0415B01L2400/0418G01N27/44704
Inventor 尤晖成伟清常永嘉孙翠敏
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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