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Test paper box for detecting salmonella typhimurium

A technology for Salmonella and typhoid, which is applied in the field of immunochromatographic test strips for single-antibody detection of bacteria and its preparation, can solve the problems of impact on human health and safety, obvious material matrix effect, poor stability, etc. Simple and cost-effective

Inactive Publication Date: 2015-01-21
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006]3. The colloidal gold labeling process is a physical adsorption process, so the stability in the liquid phase is poor;
[0007]4. Only when the gold particles gather to a certain amount, the naked eye can observe the purple-red band, and the color band has little contrast with the background, thus limiting Improve the detection sensitivity;
[0008]5. The matrix effect of different materials is obvious, and the background interference is very large
Antigen pairing process takes a long time, increasing R&D costs

Method used

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  • Test paper box for detecting salmonella typhimurium
  • Test paper box for detecting salmonella typhimurium
  • Test paper box for detecting salmonella typhimurium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Single antibody test strip structure description and preparation method of the present invention

[0031] 1. The test strip box of the present invention

[0032] The test paper box for detecting Salmonella typhimurium comprises a single antibody test strip and a fluorescent dye box; the single antibody test strip comprises a sample pad, a nitrocellulose membrane, an absorbent pad and a PVP base plate, and its specific structural features are: nitric acid The cellulose membrane is pasted on the PVP bottom plate; one end of the bottom plate is a sample pad, and the other end is a water-absorbing pad; after being cut into strips with a width of 3-4mm, it is a single antibody test strip; the nitrocellulose membrane is sprayed There is Salmonella typhimurium antibody sprayed as the detection line T line, anti-fluorescent dye antibody spray quality control line C line;

[0033] Fluorescent dye cartridges contain the fluorescent dyes propidium iodide, fluorescein...

Embodiment 2

[0044] Example 2 : Test strips after bacteria stained with other dyes

[0045] Common dyes saffron, sand yellow, crystal violet and common fluorescent dye DiOC 18 (3) After staining Salmonella typhimurium with fluorescein isothiocyanate (FITC) for 10 minutes, load the sample onto the test strip of the present invention. Spray the corresponding dye antibody on the C line;

[0046] The result is as image 3 As shown, other dyes were found to be unable to develop color on the test strip. The reason may be that these dyes are not suitable for use in test strips. When they combine with bacteria, they also combine with the nitrocellulose membrane or sample pad of the test strip, so that the stained bacteria cannot migrate on the test strip. , and finally cannot develop color. And four kinds of fluorescent dyes of propidium iodide, fluorescein diacetate (FDA), acridine orange or SYBR Green screened by the present invention can be preferably suitable for the use of test strips, ...

Embodiment 3

[0047] Example 3: Test paper box of the present invention detects Salmonella typhimurium

[0048] Get the test paper box that embodiment 1 prepares;

[0049] The number of cells taken were: blank group, 10 4 、10 5 、10 6 、10 7 、10 8 、10 10 The CFU / mL Salmonella typhimurium suspension was stained with 0.01% fluorescent dye solution for 5 min. Add 4 drops of the sample (about 100 μL) to the sample hole of the test strip after the stained bacterial liquid. After reacting for 15 minutes, judge the result under the ultraviolet light. There are two bands at the detection line, and the result is positive. There is no bright band in the detection line, and the result is negative;

[0050] Compared with the detection results of Salmonella typhimurium colloidal gold test strips with the same number of bacteria, Figure 4 The test results of colloidal gold test strips for Salmonella typhimurium showed that the minimum detection limit was 10 7 CFU / mL. The test results of the...

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Abstract

The invention relates to a test paper box which is based on a bacterial fluorescent staining technique and is used for detecting dyed salmonella typhimurium via immunochromatography by utilizing a single antibody. The test paper box comprises a single antibody test strip and a fluorochrome box. The test paper box has the advantages that the salmonella typhimurium is dyed in advance by utilizing fluorochrome and then is sprayed on a T line of the test strip by utilizing a specific antibody of the salmonella typhimurium so as to capture the salmonella typhimurium in a mixed sample, so that the salmonella typhimurium in the sample can be rapidly and specifically detected.

Description

technical field [0001] The invention relates to the technical fields of bacteriology and immunology. Specifically, the invention relates to an immunochromatographic test strip for detecting bacteria with a single antibody and a preparation method thereof. Background technique [0002] Immunochromatography (immunochromatography) is a rapid detection technology established on the basis of immunofiltration in the late 1980s and early 1990s. The antibody (or antigen) of the antigen-antibody reaction is pre-coated on the specific position of the immunochromatographic test paper in the form of a strip, and the antigen (or antibody) in the sample to be tested is pre-colored in the process of developing with the developing solution. When the antigen (or antibody) in the sample to be tested marked by color is expanded to a specific position (that is, the position where the antibody (or antigen) is coated in a strip), the antigen (or antibody) in the sample to be tested marked by col...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/56916
Inventor 徐锋魏华甘敏武晓丽叶若松陈星星王登远杨栋刘琚珥许恒毅胡海宁
Owner NANCHANG UNIV
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