The invention discloses a
genotyping method of
human papilloma virus, which carries out permutation and combination on the sequences of 25bp behind GP5 of 40 common HPV subtypes by designed
software, and the detailed steps are as follows: 1. the sequences of 25bp behind GP5 of a
single type of the 40 common HPV subtypes; 2. mixed sequence mode of 25bp behind GP5 after arbitrary two types of the 40 common HPV subtypes are combined; 3. mixed sequence mode of 25bp behind GP5 after arbitrary three types of the 40 common HPV subtypes are combined; 4. mixed sequence mode of 25bp behind GP5 after arbitrary four types of the 40 common HPV subtypes are combined; 5. mixed sequence mode of 25bp behind GP5 after arbitrary five types of the 40 common HPV subtypes are combined; and 6. mixed sequence mode of 25bp behind GP5 after arbitrary six types of the 40 common HPV subtypes are combined. All the
modes carry out
pairing with the bases following GP5 by the ATCG sequence, and the
pairing number is recorded, circulation is carried out in this way until the sequences of 25bp behind GP5 are all paired, then a
pyrosequencing mode
database infected by 1 to 6 types of different subtypes is created, the sample is carried out
DNA extraction and MY09 / 11 amplification, the amplification product is taken as a template and then carried out amplification by GP5+ / 6+, then GP5 is taken as a sequencing primer, and the sequencing result is compared with the
pyrosequencing mode
database to determine the type. The
genotyping method has the advantages of low cost, convenient operation, strong specificity and synchronously detecting a plurality of types of inflection.