Detection and parting method of human papillomavirus and reagent box
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A technology for human papillomavirus and papilloma, which is applied in the field of nucleic acid probes and kits for detection and typing of human papillomavirus, and can solve the problems of low clinical value and the like
Active Publication Date: 2007-04-18
SHANGHAI TELLGEN LIFE SCI CO LTD
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Abstract
Description
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Problems solved by technology
According to clinical needs, these main types should be distinguished, so the corresponding probes must be strictly distinguished; for other rare types, especially low-risk types, the clinical value of distinguishing each type is not great
Method used
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Examples
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Embodiment 1
[0089] One-to-one coating of probes
[0090] 1. Preparation of chips for detection of HPV6, HPV11, HPV16 and HPV18:
[0091] name
sequence
SEQ ID
NO
HPV06
5'NH 2 TTTTTTTTTTTAGACGTTCGATTTCCACTAC3′
01
HPV11
5'NH 2 TTTTTTTTTTTGGGGGTAATAACAGATCATC3′
02
HPV16
5'NH 2 TTTTTTTTTTACCTCTGATGCCCAAATA3′
09
HPV18
5'NH 2 TTTTTTTTTTCAGGTATGCCTGCTTCAC3′
11
Globin
5'NH 2 TTTTTTTTTTTCCCTGACTTTTATGCCCAG-3′
53
(positive control)
NC
(negative control)
5'NH 2 TTTTTTTTTTTCGTTAGGTCGGAGCTTGATC-3′
54
[0092] 2. Select 6 microspheres No. 31, 32, 33, 34, 35, and 36 [Luminex Company] to coat the probes according to the following method:
[0093] (1) Place 6 kinds of microspheres Nos. 31, 32, 33, 34, 35, and 36 and a portion of EDC powder stored at -20°C in
[0094] Equilibrate at room temperature for 30 minutes;
[0095] (2) D...
Embodiment 2
[0193] Probe mixed coating method
[0194] 1. Preparation of chips for detecting HPV6, HPV11, HPV53, HPV54, HPV40, HPV42, HPV43, HPV44, HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV45, HPV51, HPV52, HPV56, HPV59, HPV58 and HPV66:
[0195] HPV06
5'NH 2 TTTTTTTTTTTAGACGTTCGATTTCCACTAC 3′
HPV11
5'NH 2 TTTTTTTTTGGGGGTAATAACAGATCATC 3′
HPV53
5'NH 2 TTTTTTTTTTTTCCTCACCAATAACGC 3′
HPV54
5'NH 2 TTTTTTTTTGGAGTTGCAGCATAAATAGA 3′
HPV40
5'NH 2 TTTTTTTTTCAATAGGAGTCCGACCAG 3′
HPV42
5'NH 2 TTTTTTTTTTTGGCAGACATAATTTAGGTAGTA 3′
HPV43
5'NH 2 TTTTTTTTTTCTAATACCAGGTCCAAAAT 3′
HPV44
5'NH 2 TTTTTTTTTTTGCACTTTTAATAACCAGATCCT3′
HPV16
5'NH 2 TTTTTTTTTGAAAATAATTTGAACTGGCT 3′
HPV16
5'NH 2 TTTTTTTTTTACCTCTGATGCCCAAATA 3′
HPV18
5'NH 2 TTTTTTTTTCAGGTATGCCTGCTTCAC 3′
HPV31
5'NH 2 TTTTTTTTTTTCAGTAGGGACCGATTCAC 3′
HPV33
5'NH 2 TTTTTTTT...
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Abstract
A testing and typing method and its reagent boxes for Human Papillomavirus(HPV), relating to exciters and nucleic acid probes which are especially for testing and typing of HPV. The invention fixes three types of special probes including different subgroups of low-hazard HPV and high-hazard HPV, subgroups with extra-low infection rate of low-hazard or high-hazard HPV, onto the same position or mediator. The invention also fixes oligomeric nucleoside probes of special HPV onto microballoons based on Luminex xMAP technical platform, which forms microballoons probes for testing HPV subgroups and typing slugs of HPV. A testing and typing method with simplicity of operator and its reagent boxes are invented. The method has much strongpoint including high-sensitivity, high-flux, stable result and good repeatability.
Description
technical field [0001] The present invention relates to nucleic acid probes, in particular to nucleic acid probes for detection and typing of human papillomavirus (HPV) and a kit thereof. Background technique [0002] Human papillomavirus (HPV) is an epitheliophilic virus that is widely distributed in humans and animals and has a high degree of specificity. For a long time, HPV has been known to cause benign human tumors and warts, such as growth in Human warts vulgaris, condyloma acuminatum, and papillomas growing on mucous membranes on the skin and mucous membranes near the reproductive organs. There are about 80 types of HPV that have been identified. They are divided into skin type HPV and genital epithelial HPV according to the epithelium they infect. About 35 types can infect women's genital tract, and about 20 types are related to tumors. According to the risk of different types of HPV and tumor occurrence, it is divided into low-risk types and high-risk types of HPV...
Claims
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Application Information
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