Detection and parting method of human papillomavirus and reagent box

Abstract

A testing and typing method and its reagent boxes for Human Papillomavirus(HPV), relating to exciters and nucleic acid probes which are especially for testing and typing of HPV. The invention fixes three types of special probes including different subgroups of low-hazard HPV and high-hazard HPV, subgroups with extra-low infection rate of low-hazard or high-hazard HPV, onto the same position or mediator. The invention also fixes oligomeric nucleoside probes of special HPV onto microballoons based on Luminex xMAP technical platform, which forms microballoons probes for testing HPV subgroups and typing slugs of HPV. A testing and typing method with simplicity of operator and its reagent boxes are invented. The method has much strongpoint including high-sensitivity, high-flux, stable result and good repeatability.

Description

technical field

[0001] The present invention relates to nucleic acid probes, in particular to nucleic acid probes for detection and typing of human papillomavirus (HPV) and a kit thereof. Background technique

[0002] Human papillomavirus (HPV) is an epitheliophilic virus that is widely distributed in humans and animals and has a high degree of specificity. For a long time, HPV has been known to cause benign human tumors and warts, such as growth in Human warts vulgaris, condyloma acuminatum, and papillomas growing on mucous membranes on the skin and mucous membranes near the reproductive organs. There are about 80 types of HPV that have been identified. They are divided into skin type HPV and genital epithelial HPV according to the epithelium they infect. About 35 types can infect women's genital tract, and about 20 types are related to tumors. According to the risk of different types of HPV and tumor occurrence, it is divided into low-risk types and high-risk types of HPV...

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