66 results about "Hpv detection" patented technology

Embodiments of the invention provide methods, assays, and kits for detecting HPV infection, including infection by various HPV genotypes, early and / or late HPV-associated or HPV-specific proteins or antibodies. Detection of HPV DNAs, genomes, and / or oncoproteins by protein chips immunological assays can be used in early clinical screening for HPV infection and general diagnosis for cervical cancer and can be advantageous performed in a multiplexed test. Comparative detection of altered levels of HPV proteins and host proteins can performed in one or more assays. The polypeptides, recombinant proteins, antibodies, nucleic acids, and various detection methods thereof are particularly useful for diagnosing carcinomas of the uterine cervix and those at risk of developing cervical cancer.

The present invention provides a method and a kit for determining whether a head and neck cancer is HPV-related. In one embodiment, an RNAscope® HPV assay was designed to detect the presence of E6 / E7 mRNA of certain high-risk HPV subtypes related to head and neck cancer. The present invention also provides a method and a kit for determining whether a cervical lesion is a benign lesion or a cervical intraepithethial neoplasm lesion. The present invention further provides a method for determining the progression of cervical intraepithethial neoplasm based on the spatial pattern and levels of the E6 / E7 mRNA of certain high-risk HPV subtypes. The present invention also provides a method for determining the risk of developing cervical cancer in a human diagnosed with cervical intraepithethial neoplasm based on presence and absence of the certain subgroups of high-risk HPV subtypes.

A testing and typing method and its reagent boxes for Human Papillomavirus(HPV), relating to exciters and nucleic acid probes which are especially for testing and typing of HPV. The invention fixes three types of special probes including different subgroups of low-hazard HPV and high-hazard HPV, subgroups with extra-low infection rate of low-hazard or high-hazard HPV, onto the same position or mediator. The invention also fixes oligomeric nucleoside probes of special HPV onto microballoons based on Luminex xMAP technical platform, which forms microballoons probes for testing HPV subgroups and typing slugs of HPV. A testing and typing method with simplicity of operator and its reagent boxes are invented. The method has much strongpoint including high-sensitivity, high-flux, stable result and good repeatability.

The invention provides a human papillomavirus HPV DNA fragment, a mix primer for human papillomavirus HPV DNA detection and / or parting and application thereof. The mixed primer can specifically amplify to obtain one or a plurality of DNA sequences from HPV6, HPV 11, HPV 16, HPV 18, HPV 26, HPV 31, HPV 33, HPV 35, HPV 39, HPV 40, HPV 42, HPV 43, HPV 44, HPV45, HPV 51, HPV 52, HPV 53, HPV 54, HPV 55, HPV 56, HPV 58, HPV 59, HPV 61, HPV 66, HPV 68, HPV 70, HPV 72, HPV 73, HPV 81, HPV 82 and HPV 83, can be used for the human papillomavirus HPV DNA detection and / or parting and prepared into a PCR kit. The invention can detect common types of HPV at a time, greatly improve the HPV detection efficiency, overcome the defect of missed detection of latent infection by a serology and IHC detection method, realize early diagnosis of HPV diseases, and is suitable for clinical HPV gene detection and parting and guides prevention and cure of cervical carcinoma.

The invention relates to a detection method of human papilloma virus (HPV), in particular to the HPV detection method based on the Solexa sequencing method.

The invention discloses a nanometer graded antibiotic antiviral condom, which is characterized by the following: grafting interferon on the condom, improving sterilizing activity from 2.1% to 6.3%.

The invention discloses a gene chip for detecting human papillomavirus (HPV), comprising a solid carrier and a human papillomavirus detecting probes fixed on the solid carrier. The detecting probes comprise nucleotide sequences in SEQ ID No.9-37, and the 27 types of HVP subtype specific probes in the invention correspondingly comprise 22 kinds of high-risk and mediate-risk HPV, and 5 kinds of low-risk HPV and two kinds of high-risk HPV integration (HPV16 / HPV18). The invention also discloses a detecting kit for detecting various subtypes of HPV in a sample with high speed and high flux, and the kit comprises the gene chip, a primer sequence for detection, a human GAPDH gene, special HPV lysing solution, PCR reaction liquid and a color reagent, wherein the detecting probe fixed on the gene chip has the nucleotide sequence in the SEQ ID No.9-37; the primer sequence for detection is used for amplifying the DNA sequence of the HPV in a clinical sample, and the human GAPDH gene is used for amplifying the clinical sample. The HPV detection has important significance for the clinical diagnosis of HPV as well as the early prevention, the diagnosis, the treatment and the postoperation tracking of cervical cancer.

The invention discloses a qPCR primer for detecting HPVs. The qPCR primer comprises the following primer pairs of two types: the primer pairs of the first type are capable of amplifying conserved sequences in the HPV genomes of all the types; the primer pairs of the second type are capable of detecting the HPVs of at least two types in the same tubular qPCR system. The invention also discloses an HPV detection kit comprising the primer pairs. The HPV detection kit is high in HPV detection specificity and completely reaches the specificity level of qPCR by use of a probe method. Compared with traditional PCR diagnosis, the HPV detection kit is simpler and more reliable to operate, and meanwhile, applicable to high-throughput sample detection. The HPV detection kit further has the characteristics of high specificity, strong sensitivity and low cost by applying a double-stranded DNA combined fluorochrome qPCR detection method to the diagnosis of the HPVs; as a result, a quick and accurate molecular detection method is provided for general investigation of the HPVs and prevention and treatment of the cervical cancer, and meanwhile, the test cost is greatly reduced; in short, the HPV detection kit has an important popularization and application value.

The invention relates to the technical field of biological detection, in particular to HPV (human papilloma virus) typing detection primers as well as a detection method and an application thereof. QPCR primers for typing detection of HPV are shown as SEQ ID NO:1-38; the invention also discloses an application of the qPCR primers for typing detection of the HPV in preparing an HPV detection kit. The specificity and the sensitivity of the primer pairs and the kit provided by the invention in HPV typing detection are greatly higher than that of a common reverse dot blot method, and the primer pairs and the kits are relatively low in requirements on environment and operation and are broad in application. Meanwhile, according to the invention, a DNA double-stranded chimeric fluorescent dye qPCR detection method is applied to the HPV typing detection; the detection specificity is improved based upon double analysis through an amplification curve and a melting curve, so that the specificity can reach the detection level of a probe method, and meanwhile, cost can be greatly reduced; the primers have an important significance for the promotion of HPV general survey and for the prevention and treatment of cervical carcinoma; and the primers are quite high in popularization and application values.

The invention discloses a quick and accurate HPV (human papillomavirus) immunochromatographic detection test strip, a method for preparing the same and a HPV detection method on the basis of immunochromatographic technologies. The immunochromatographic technologies are applied to detecting HPV antigen protein for the first time, and accordingly the HPV immunochromatographic detection test strip has high-specificity and high-sensitivity detection performance. Compared with Pasteur and HPV-DNA (deoxyribonucleic acid) detection reported in existing literature, the HPV immunochromatographic detection test strip, the method and the HPV detection method have the main advantages that the HPV immunochromatographic detection test strip and the HPV detection method are short in detection time (5-20 minutes); optional special instruments can be omitted; the HPV immunochromatographic detection test strip is easy and convenient to operate and low in detection cost, only one-step reaction is required, and operators do not need to be trained; special temperature requirements and freezing can be omitted, and accordingly the HPV immunochromatographic detection test strip is convenient to store and transport and can be stored at the room temperatures for 24 months.

The present invention relates to amplification primers and detection probes, which are useful for the detection of human papillomaviruses (HPV), and more particularly of HPV, which can be oncogenic for the mucosal epithelia. The amplification and detection systems provided by the present invention are group-targeted systems, namely A5-, A6- A7-, and A9-targeted systems. The amplification and detection systems of the invention allow for an amplification of HPV in multiplex as well as for a real-time detection, whereby at least the thirteen HR HPV can be detected in a single-tube assay. The invention further allows for a reliable quantitation of HPV viral loads in real-time multiplex amplification.

The invention belongs to the technical field of biology, and discloses a cell preservation solution as well as a preparation method and application thereof. The cell preservation solution comprises alcohol, acid, buffer salt, an ionic strength maintaining agent, an antioxidant and proteolytic enzyme. According to the cell preservation solution, samples of multiple mucus and blood cells can be well treated, and the staining effect of thin-layer liquid-based cytology detection is improved; however, the proteolytic enzyme can influence the staining effect of reductive blue tissue cells, the influence of the proteolytic enzyme on the staining of the reductive blue tissue cells is reduced by adding the antioxidant, and meanwhile, the accuracy and the stability of immune cell chemical staining and reductive blue tissue cell staining are improved; and moreover, the cell preservation solution has no influence on the accuracy and stability of HPV detection after preserving a cell sample. The cell preservation solution can meet requirements of four screening methods including thin-layer liquid-based cytology detection, HPV detection, immunocytochemical staining and vat blue tissue cell staining at the same time.

The invention provides a test strip for a rapid cervical cancer HPV detection device. The test strip comprises a bottom plate, a sample pad, a bonding pad and an absorption pad, wherein the sample pad is arranged above on end of the bottom plate; the absorption pad is arranged above the other end of the bottom plate; the bonding pad is overlapped below one end of the sample pad; a nitrocellulose membrane pad is arranged between the bonding pad and the absorption pad; a test line and a control line are arranged on the nitrocellulose membrane pad; and nucleic acid probes are respectively arranged on the test line and the control line. The test strip has the advantages that 1, the sensitivity is high, namely according to the design of the nucleic acid probes, the sensitivity of the detection method can be greatly improved during application; and 2, the specificity is high, compared with high nonspecific binding interference of antigens-antibodies, due to the design of the specific nucleic acid probes, the nonspecific action in a sample can be greatly reduced during actual application.

The invention discloses a human papillomavirus quick detection method, a liquid-phase chip and a kit. The liquid-phase chip comprises microsphere carriers, labeled molecules and nucleic acid capture probes aiming at different HPV types, the microsphere carriers are connected with coupling groups, the labeled molecules are available for independent detection, each capture probe comprises a specificnucleotide sequence and at least one coupling group, and each specific nucleotide sequence and target nucleic acid molecules coded by different types of HPV viruses form complementary pairs. By adoption of the method, accurate quantitative marking of the microsphere carriers can be realized, and stability and distinguishability in marking of the same type of microsphere carriers are remarkably improved. On the basis of the chip, the invention further provides a HPV detection method and a kit, 24 common HPV types can be detected in one time, the defect of detection omission in application of serology and immunohistochemical methods can be overcome while HPV detection efficiency is greatly improved, and early detection of the HPV viruses is realized.

The invention relates to the field of pharmaceutical technology, particularly to a spray for treating diseases caused by human papilloma viruses and a preparation method. The spray comprises, by volume ratio, 1 ml of rabies purified vaccine for human use and 9 ml of chitosan-acetic acid solution with a prepared mass volume concentration being 0.01 g / ml to 0.03 g / ml, wherein the chitosan-acetic acid solution is prepared by fully dissolving 10g to 30g chitosan and the acetic acid solution until the volume reaches 1000 ml. The preparation method comprises the steps of first preparing 0.3% to 1.0% of the acetic acid solution and then preparing the chitosan-acetic acid solution; adding 1 part of the rabies purified vaccine for human use with an active content being 5 IU to 20 IU by volume to a measuring flask with a volume of 10 parts, adding the chitosan-acetic acid solution, and diluting for preparing the vaccine solution. The spray and the preparation method thereof in the invention is focused on follow-up treatments (Tc subgroup detection, HPV detection and improvement of clinical symptoms) of cervical carcinoma patients infected by HPV, HPV-infected people, and venereal diseases, cervical erosion and chronic cervicitis caused by HPV infection, and achieves good clinical results.

The invention discloses a composition for detecting and typing high-risk HPV, a kit and a method and relates to the field of molecule biology detection, in particular to the field of HPV detection. The composition can be used for specifically detecting E6 / E7 mRNA of HPV types 16, 18, 31, 33, 35, 39, 45 ,51, 52, 53, 56, 58, 59, 66 and 68. At the same time, the invention also provides application ofthe composition in the detection of HPV E6 / E7 mRNA, the kit comprising the composition and the method for detecting the HPV E6 / E7 mRNA.

The invention discloses a reagent for detecting a human papillomavirus (HPV). The reagent provided by the invention comprises a primer composition A and a primer composition B, wherein the primer composition A consists of DNA shown in a sequence table from sequence 1 to sequence 18; and the primer composition B consists of DNA shown in the sequence table from sequence 20 to sequence 43. The reagent can be used for assisting in identifying the HPV. The reagent provided by the invention can detect all the types of the HPV in an infected female genital tract, wherein the HPV comprises 14 high-risk types of HPV and 12 low-risk types of HPV. The reagent mainly solves the technical problem that the infected HPV cannot be typed or the typing is not accurate caused by a cross reaction in the conventional detection method so as to provide an accurate, high-sensitivity, economic and practical detection method which is suitable for clinical HPV detection and large-scale crowd HPV screening.

The invention belongs to the field of bioengineering, and specifically relates to a kit for detecting HPV (Human Papillomavirus). The kit is prepared from a primer for amplifying the HPV as well as Uvs X, Uvs Y, SSB and DNA polymerases. In the kit for detecting the HPV, provided by the invention, a T4-series enzyme combination of Uvs X and Uvs Y is selective for replacing existing recombinase. Theenzyme system not only is capable of further increasing the sensitivity and the specificity of HPV detection, but also can be more diversified in selecting primers for amplifying the HPV, and 100 to200 bp products can be amplified under a shorter primer condition, so that the reaction time can be shortened, the interference of a background signal can be reduced, the detection efficiency can be finally increased, and the detection cost can be remarkably reduced.

The invention discloses an HPV detection kit, which comprises a kit body, wherein a sealing cover is arranged on the top surface of the kit body, a horizontal plate is arranged at the middle part of an inner cavity of the kit body, a plurality of round through holes are formed in the top surface of the horizontal plate, a bottom-sealed mounting tube is connected to the bottom of each round throughhole, and is used for placing a sample tube, and a sealing hood is arranged at the top of each round through hole, and is used for sealing the part, exposed from the horizontal plate, of the top of the corresponding sample tube. According to the kit, pollutions and interference between different samples collected during physical examination can be avoided, and the disease detection accuracy can be improved.

The invention relates to the field of molecules biological detection, in particular to the field of HPV detection, and provides a composition. HPV16 / 18 can be subtyped, and HPV 16, 18, 31, 33, 35, 39,45, 51, 52, 53, 56, 58, 59, 66 and 68 types can also be specially detected. Besides, the invention further provides an application of the composition for detecting HPV, the reagent kit containing thecomposition, and the method for detecting the HPV.

The disclosure provides compositions and methods for the detection of human papillomavirus (HPV) nucleic acids in a urine sample from a human subject. The nucleic acids may be from one or more high risk forms of HPV. The urine sample is minimally processed, without fractionation into cell-free and cell-containing portions, before preparation of the nucleic acids for detection of HPV sequences.

The invention discloses a probe and a detection method for HPV (Human Papillomavirus) detection. The preparation process of the probe requires two rounds of PCR reactions. The NGS technology-based detection method comprises the steps of preparing a DNA template, performing fragmentation on genomic DNA, purifying a fragmented product, constructing a genomic library, performing PCR amplification onthe genomic library, hybridizing the probe with the genomic library, capturing a hybridization product by magnetic beads, and performing high-throughput sequencing on the captured product and analyzing whether a sample to be tested is infected with HPV, the infection degree of the HPV, the integration location and the pathogenic mechanism of the HPV and the like. The probe and the method disclosedby the invention have the advantages of low cost, fast data analysis, high coverage depth, high detection accuracy and the like, and are suitable for early screening and postoperative diagnosis of diseases caused by the HPV, and provide guidance for personalized medication.

Various sequences as detection probes for human papillomavirus (HPV), in which each of these sequences is able to detect multiple subtypes of HPV, are provided. HPV detection chips, detection kits comprising these probes and HPV detection method by using these probes are also provided. With the sequences of the current invention, it is possible to detect commonly seen 38 subtypes of HPV, including those of high risk, with various combinations of the sequences of the current invention.

The invention discloses a method of detecting a human papilloma virus 16 / 18 type gene in urine by using digital PCR (polymerase chain reaction). The method comprises the particular steps of extracting DNA (deoxyribonucleic acid) from the urine, preparing a primer reaction solution, preparing a droplet, performing PCR amplification, detecting the droplet, analyzing data and displaying a result, wherein a lysate comprises 10mM of Tris-HCl, 1mM of EDTA (ethylene diamine tetraacetic acid) and 0.5% of SDS (sodium dodecyl sulfonate); a pH (potential of hydrogen) value of a sodium acetate solution is 5.2. The detection mode is free from age limit and available; sampling is noninvasive; the method has high sensitivity and high precision and overcomes the detection difficulty arising from little viral nucleic acid in the urine; HR-HPV detection in the urine can be widely applied to clinical diagnosis and treatment and cervical cancer screening; the method has the advantage that the method has an important significance for prevention and treatment of a cervical cancer.

The invention provides a method for detecting HPV (human papillomavirus) nucleic acid in a biological sample. The method comprises the following steps of (i) respectively using 14 types of HPV specific primer pairs and primers in a probe group to amplify nucleic acid segments in a sample; (ii) using the amplified segments in step (i) to respectively touch the 14 types of HPV gene specific primer pairs and the probes in the probe group; (iii) detecting signals released by the probes, and judging the existence of the HPV in the biological sample and the type of the HPV. The invention also provides a kit using the method to detect the HPV nucleic acid in the biological sample.

The invention is directed to the detection of multiple types of HPV with high specificity and high sensitivity. The invention provides primer sets, probes, and a kit containing the primer set and the probe, for type-specific HPV detection.

The invention discloses a method for constructing recombinant plasmid of a human papilloma virus (HPV) subtype L1 gene. The problem that the HPV detection result of interference performance is liableto occur due to lack of various subtype L1 positive control nucleic acid standard samples at present is solved. The method for constructing the recombinant plasmid of the human papilloma virus (HPV) subtype L1 gene is characterized in that an NCBI database finds a gene sequence of different subtype L1 areas of HPV; the gene sequence is subjected to bioinformatics analysis by a BLAST function on awebsite; a GP5<+> / 6<+> area is selected as a target gene target fragment; 25 groups of specific primers are designed separately by using Primer Premier 5.0 software; a corresponding positive sample issubjected to PCR amplification; a amplification product is used for establishing standard plasmid, construction work of 25 subtype recombinant standard plasmid of the HPV is finished successfully; and guarantee is provided for the stability of the test result with high-risk HPV infection or not which is screened by a molecular biological technology of HPV nucleic acid detection.

The present invention discloses using SPR technology to simultaneously and qualitatively detect different HPV genotypes. It also discloses an efficient formula to make a mixed SAM that can greatly enhance the immobilization ability of the metal surface in SPR based techniques, which is good for the immobilization of HPV specific DNA probes used for the detection of different HPV genotypes.

The present invention relates to a method for self-collecting intravaginal sample for HPV test. The self-collecting intravaginal sample apparatus of the present invention comprises: an open end part, a closed end part, a tube providing an inner space that a finger could enter to insert an apparatus into women's vagina, a collecting part collecting intravaginal sample and attached outer surface of the closed end of the tube, and a turner preventing the collecting part being contaminated from an outer contaminant; wherein the collecting part collects a sample from the vagina by turning over the tube inside out so that collecting part may place inside of the tube.

The invention discloses a cervical / vaginal cast-off cell self-harvesting device. The device comprises a sample bottle and a sampler. The sample bottle comprises a bottle cap, a bottle body and solid-state filter paper. A cone part is arranged on the inner lower portion of the bottle body, and the solid-state filter paper is matched with the cone part in appearance and attached to the cone part in a lining mode. After the sampler harvests cervical / vaginal cast-off cells, the sampler is placed inside the sample bottle, the head portion of the sampler is placed inside the cone part, and the head portion of the sampler makes tight contact with the periphery of the solid-state filter paper. By means of the cervical / vaginal cast-off cell self-harvesting device, the DNA / RNA of the harvested cells can be reserved to the maximum degree, hence, HPV detection sensibility is greatly improved, and the screening mode that 'cervical / vaginal cell self-sampling high-risk HPV detection is turned into cervical cancer preliminary screening' can be improved; in addition, the cervical / vaginal cast-off cell self-harvesting device can also be used for storing the DNA / RNA of the cells at an indoor temperature, the self-harvesting device is small in size and can be sent, and thus central detection is facilitated.