6 results about "Hpv detection" patented technology

The invention relates to the technical field of oil-control shampoo, and particularly discloses a test strip for early screening of HPV nucleic acid and a preparation method thereof.The test strip comprises a PVC backboard, a sample pad and a water absorption pad are arranged at the two ends of the top of the PVC backboard respectively, an NC film is arranged in the middle of the top of the PVC backboard, one side of the top of the NC film is in extrusion contact with the bottom of the water absorption pad, and the other side of the top of the NC film is in extrusion contact with the bottom of the water absorption pad; a combination pad is arranged on the other side of the top of the NC membrane in an extrusion contact manner, and one side of the top of the combination pad is in extrusion contact with the bottom of the sample pad. The invention provides a test strip for early screening of HPV (human papilloma virus), which aims at HPV nucleic acid and is developed on the basis of a colloidal gold technology. The test strip is used for solving the problems of complicated operation, dependence on professional instruments and personnel, high detection cost, incapability of meeting the requirement on convenience of early screening and the like in the existing HPV detection technology. Through the method, rapid and accurate detection of the HPV can be realized, and the method is suitable for large-scale early screening including scenes such as home self-inspection and the like.

The application discloses a quality control product for human papillomavirus molecular detection and a preparation method thereof, and belongs to the technical field of biological products. After synthesizing 25 types of HPV full genomes of high-risk types (HPV16, HPV18, HPV26, HPV31, HPV33, HPV35, HPV39, HPV45, HPV51, HPV52, HPV53, HPV56, HPV58, HPV59, HPV66, HPV68, HPV73, HPV82) and low-risk types (HPV6, HPV11, HPV42, HPV43, HPV44, HPV81, HPV83), the HPV full genomes are connected into a pcDNA3.1(+) skeleton vector system, the HPV full genomes are integrated into the genome of a human ovarian cancer cell line ovcar3 respectively, and 25 strains of human ovarian cancer cell lines stably integrating the HPV full genomes are obtained. The new quality control product or kit of various types of simulated clinical samples such as genomic DNA, cell precipitate and cell suspension can be prepared and is suitable for various detection platforms. The human cell line quality control product stably integrating the full-length genomes of the 25 HPV types can more accurately determine the viral load and control the whole process of HPV detection.

This invention relates to the field of biological detection, and more particularly to primer combinations, reagent kits, and their applications. The invention provides primer combinations including: primer set I, primer set II, primer set III, primer set IV, primer set V, primer set VI, primer set VII, primer set VIII, primer set IX, primer set X, primer set XI, primer set XII, primer set XIII, primer set XIV, primer set XV, primer set XVI, primer set XVII, primer set XVIII, primer set XIX, primer set XX, primer set XXI, primer set XXII, and primer set XXIII. This invention also provides a reagent kit for rapid HPV detection. This kit can rapidly detect the above 23 HPV types within 20 minutes. The detection limit of the kit is up to 10. 3 The accuracy and specificity are good, with a per-copy / mL ratio.

The invention discloses a preparation method of a kit for detecting human papilloma, and particularly relates to the technical field of gene fragment detection.The kit comprises a micro-fluidic chip, independent reaction units are arranged in the chip in a partitioned mode, and each unit comprises a primer group for specific subtypes of HPV, isothermal amplification enzyme, a CRISPR-Cas12a compound and a quantum dot fluorescent probe; the freeze-drying reagent comprises the primer group wrapped by a freeze-drying protective agent, an isothermal amplification enzyme, a CRISPR-Cas12a (clustered regularly interspaced short palindromic repeats-associated 12a) compound and a quantum dot fluorescent probe. Through cooperation of microfluidic integration, isothermal amplification, CRISPR verification and quantum dot labeling, the bottleneck of traditional HPV detection in sensitivity, specificity and convenience is broken through, meanwhile, the clinical diagnosis value is improved through the double-target design, and the application scene is expanded through the freeze-drying process.

The application discloses a HPV virus detection kit, which improves the problems of traditional HPV detection, such as the need of expensive instruments, complicated operation and long detection time, by adopting LAMP technology and visual detection design; the kit contains a freeze-drying tube, a liquid reaction tube and a sample lysis tube, LAMP primer groups in the freeze-drying tube are combined with Bst DNA polymerase to realize constant temperature amplification; when the MOPS buffer microspheres in the liquid reaction tube release components to maintain the reaction environment at pH change, the accuracy and stability of the chromogenic accuracy of neutral red are protected, and the neutral red is used as an indicator to intuitively present the result through color (orange yellow negative, red positive); after the sample is mixed with the lysis solution and heated, the reaction system is added at 60 DEG C constant temperature for 30 min, and naked eye judgment can be carried out, without the need of a thermal cycler and other equipment, so that the HPV screening is effectively promoted in the popularization and application of primary medical institutions.

The invention provides an intelligent HPV (human papillomavirus) typing detection system and method, and relates to the technical field of HPV detection. The intelligent HPV typing detection system comprises a hardware assembly and a software module, the hardware assembly comprises a sample collection tool, a PCR amplification instrument, a chip scanner, a computer and auxiliary equipment, and the software module comprises an image analysis module, a data processing module, a result report generation module and a system control module. By utilizing artificial intelligence and an image recognition technology, an HPV gene chip image can be accurately analyzed, and a system can learn a large number of HPV positive and negative sample image features through a deep learning algorithm, so that a positive region in the chip image can be accurately recognized, fine features in the image can be automatically recognized, and the detection accuracy is improved. The subjectivity and errors of manual judgment are avoided, and the detection accuracy is greatly improved.