29 results about "Hpv detection" patented technology

The invention discloses a cervical LSIL progress risk prediction method and system based on multi-modal time sequence fusion, and the method comprises the steps: collecting multi-modal data, and carrying out the standardization processing; extracting dynamic change characteristics in continuous annual TCT liquid-based pictures through a convolutional neural network, and positioning a high-risk cell region; carrying out interval sensing position coding on HPV detection records, constructing an inter-modal causal attention mechanism, and establishing a time sequence causal relationship between HPV infection events and cell abnormal evolution; a discrete time competition risk model is adopted, the progression, regression and maintenance probabilities of different time periods in the future after primary diagnosis are synchronously output, a time-varying covariable LSTM is introduced, and the weight of patient features changing along with time is dynamically updated; and generating a cell evolution thermodynamic diagram, marking a high-risk area space-time evolution path, outputting a time influence curve, and marking a key risk accumulation time window. According to the invention, accurate quantitative evaluation of the cervical low-level lesion progress risk is realized.

The invention provides a method and device for directly carrying out HPV prediction by utilizing a cervical cell pathology image, electronic equipment, a storage medium and a program, and relates to the technical field of medical image processing, and the method comprises the following steps: S10, data acquisition: obtaining a same-period cervical cell pathology full-slide image of a detected person which has been subjected to cervical tissue pathology analysis and a conclusion of which is evaluated as ASCUS; and S20, lesion area positioning: determining an abnormal cell area in the cervical cell pathological full-slide image by using the trained target detection model. Compared with the prior art, the method has the following beneficial effects: firstly, suspicious cells are screened out through a target detection method, and an existing method in a tissue pathology all-slide image is migrated into a cell pathology image; secondly, due to application of unsupervised learning and an attention mechanism, the model can automatically learn and emphasize the most important features for HPV detection in the image; and thirdly, the HPV infection state of the ASCUS patient is directly and accurately predicted from the cervical image.

The invention discloses a cervix uteri and vagina exfoliated cell sampling device for self-sampling HPV (human papillomavirus) detection, which comprises a handle, one end of the handle is fixedly connected with a spherical head body, a sampling brush head is fixedly arranged on the outer side of one end of the spherical head body of the handle, and sampling brushes are uniformly arranged on the surface of the spherical head body. The outer side of the handle is sleeved with a slidable telescopic sleeve shell, and when sampling operation needs to be carried out, the telescopic sleeve shell can slide away from one side of the sampling brush head and one side of the sampling brush brush by sliding the telescopic sleeve shell, so that the sampling brush head and the sampling brush brush are exposed to be used for collecting cervical and vagina exfoliated cells; in a non-sampling state, the sampling brush head and the sampling brush can be sleeved with the telescopic sleeve shell, so that a protection effect is achieved; compared with a traditional sampling brush or cotton swab, the contact area between the sampling brush or cotton swab and the cervix uteri and the vagina wall is greatly increased, the area where pathological cells possibly exist can be covered more comprehensively, the risk of missing sampling is reduced, and the accuracy of a detection result is improved.

The invention relates to the technical field of PCR (Polymerase Chain Reaction) detection, and discloses a high-risk human papilloma virus DNA (Deoxyribose Nucleic Acid) multiplex PCR detection kit, which adopts a primer probe group and comprises a PCR mixed solution, a negative reference substance and a positive reference substance, wherein the PCR mixed solution comprises a primer probe group. Female first-stage urine is used as a detection sample, and compared with a cervix uteri sample, sampling is more convenient, the experience feeling is better, and the willingness of female cervical cancer screening can be provided; meanwhile, the content of cervical exfoliated cells in the urine is also increased by using the first-stage urine, and the clinical sensitivity of clinical HPV detection can be remarkably improved by matching with a provided cell enrichment method.

The utility model provides an HPV (human papillomavirus) detection kit, which belongs to the technical field of kits and comprises a first kit body. A mounting frame is mounted at the upper end of the first box body through bolts, a groove is formed in the mounting frame, a top plate is mounted in the groove through bolts, two sliding grooves are formed in the top plate, two toothed plates are mounted at the lower end of the top plate through screws, two sliding seats are mounted at the upper end of the top plate, and two round rods are fixed to the lower ends of the sliding seats; the positioning groove is formed in the positioning plate, the upper end of the storage pipe is positioned through the positioning groove, the supporting block is installed on the connecting base through the bolt, the groove is formed in the supporting block, the lower end of the storage pipe is located in the groove in the supporting block, the lower end of the storage pipe is positioned through the groove in the supporting block, and the storage pipe is prevented from shaking; and the stability of the storage pipe is improved.

The application develops a one-tube RPA-CRISPR / Cas13a detection system and application in high-risk HPV typing detection, which is a one-tube HPV detection system based on recombinase polymerase amplification (RPA) and CRISPR / Cas13a, named H-RC13a-OneTube. Compared with two-step method, the system simplifies the operation process, effectively avoids the risk of aerosol pollution and RPA product loss, and realizes the complete integration of nucleic acid amplification and detection. Taking the clinically high-risk types HPV16, 18 and 52 as the verification object, the sensitivity result shows that the detection lower limit of the system for HPV16 and 52 types reaches the single copy level. The system does not need complex instruments, and the operation is simple and rapid, which provides a new technical scheme for the on-site rapid typing detection of HPV16, 18 and 52, and lays a foundation for the subsequent expansion of multi-subtype detection and clinical conversion application.

The utility model discloses an HPV (human papillomavirus) detection sampling tube and belongs to the technical field of HPV detection. Comprising a sampling pipe, the top of the sampling pipe is provided with a push rod, the bottom of the push rod is provided with a rubber block, and the circumferential outer wall of the rubber block is provided with a plurality of sealing rings. After the swab is used for sampling, the fixed ball at the top of the swab is embedded into the bottom of the mounting seat, then the swab is inserted into the sampling tube and is fully mixed with detection liquid in the sampling tube, the rubber plug is separated from the liquid outlet nozzle by rotating the sealing block, then the push rod is pushed, the detection liquid in the sampling tube is squeezed out, and detection is directly carried out; the detection liquid can be prevented from being in contact with air to cause pollution to the detection liquid; the symmetrically arranged rubber sheets can buffer the shell, the rubber sheets are arc-shaped, so that the buffering effect of the shell is better, and meanwhile, the spring arranged at the top of the base can buffer the shell again, so that the sampling tube can be prevented from being damaged when the sampling tube falls off.

The invention discloses a lateral flow immunochromatography kit based on OR logic gate combined catalysis hairpin self-assembly and application, and belongs to the field of HPV nucleic acid detection. The HPV detection probe comprises a probe H1, the nucleotide sequence of which is SEQ ID NO.1, SEQ ID NO.5, SEQ ID NO.9 or SEQ ID NO.13, a probe H3, a probe H4, a probe H5, a probe H6, a probe H7 and a probe H8, the nucleotide sequence of the probe H2 is as shown in SEQ ID NO.2, SEQ ID NO.6, SEQ ID NO.10 or SEQ ID NO.14, and the nucleotide sequence of the probe H2 is respectively matched with the SEQ ID NO.1, the SEQ ID NO.5, the SEQ ID NO.9 and the SEQ ID NO.13 in the corresponding probe H1; the nucleotide sequence of the probe H3 is SEQ ID NO.3, SEQ ID NO.7, SEQ ID NO.11 or SEQ ID NO.15, and the nucleotide sequence of the probe H3 is SEQ ID NO.3, SEQ ID NO.7, SEQ ID NO.11 or SEQ ID NO.15 and is matched with SEQ ID NO.1, SEQ ID NO.5, SEQ ID NO.9 and SEQ ID NO.13 in the corresponding probe H1 respectively.

The utility model relates to the technical field of HPV (human papillomavirus) detection devices, in particular to an HPV detection sample storage device which comprises a storage box and a storage mechanism, quick release structures are respectively arranged at the central positions of the inner walls of the front side and the rear side of the storage box, and the storage box is connected with the storage mechanism in a quick release manner through the two quick release structures. Clamping mechanisms are symmetrically arranged on the upper portions of the outer walls of the left side and the right side of the storage box correspondingly, limiting assemblies are symmetrically arranged on the lower portions of the outer walls of the left side and the right side of the storage box correspondingly, and during use, storage mechanisms containing sponge blocks of different sizes can be rapidly installed in the storage box through mutual cooperation of all structures in a rapid disassembly structure; and the stability of the storage mechanism mounted in the inner cavity of the storage box is ensured.

The invention discloses an accurate early warning auxiliary diagnosis model system based on high-risk HPV positive, and relates to the technical field of medical diagnosis, according to the accurate early warning auxiliary diagnosis model system based on high-risk HPV positive, by obtaining the relevance and action intensity of conventional blood and urine test indexes and high-risk human papilloma virus (HR-HPV) infection, the accurate early warning auxiliary diagnosis model system based on high-risk HPV positive can be used for early warning of high-risk human papilloma virus (HR-HPV) infection. A basis is provided for deep research in the field; an HR-HPV infection positive early warning model taking health screening big data as a basis and hematuria biological indexes as elements is formed, and the problems that HPV detection depends on subjective judgment of doctors or active selection of clients, an accurate measurement basis is lacked, and the screening coverage rate is not enough are solved. Therefore, a simple, convenient, rapid and economical means is provided for gynecological evaluation, reliable indications are provided for HPV detection, the screening efficiency is improved, and help is provided for prevention of HPV infection and cervical diseases.

The invention relates to the field of HPV (human papillomavirus) kits, in particular to an isothermal amplification rapid HPV detection kit which comprises a kit body, a kit cover, a refrigerator, a cooling cylinder and the like. A battery is arranged in the box body; the box body is detachably connected with a box cover; a plurality of refrigerators are fixedly connected in the box body; the box body is connected with cooling cylinders matched with the refrigerators in number. Through cooperation of a refrigerator, a cooling cylinder, a plug and a pushing system, only the refrigerator corresponding to the cooling cylinder filled with samples works, so that the problem that a large amount of energy is wasted due to the fact that an existing kit can only cool the whole interior and cannot independently cool test tubes filled with samples is solved, and meanwhile, the energy consumption is reduced. According to the kit, the prompt plate is matched with the pushing system, so that only the prompt plate corresponding to the cooling cylinder filled with the sample is exposed outside the kit body, and the problem that when an existing kit is used, a medical worker easily takes a test tube filled with the sample, namely the medical worker easily takes the test tube repeatedly is solved.

The invention relates to the technical field of oil-control shampoo, and particularly discloses a test strip for early screening of HPV nucleic acid and a preparation method thereof.The test strip comprises a PVC backboard, a sample pad and a water absorption pad are arranged at the two ends of the top of the PVC backboard respectively, an NC film is arranged in the middle of the top of the PVC backboard, one side of the top of the NC film is in extrusion contact with the bottom of the water absorption pad, and the other side of the top of the NC film is in extrusion contact with the bottom of the water absorption pad; a combination pad is arranged on the other side of the top of the NC membrane in an extrusion contact manner, and one side of the top of the combination pad is in extrusion contact with the bottom of the sample pad. The invention provides a test strip for early screening of HPV (human papilloma virus), which aims at HPV nucleic acid and is developed on the basis of a colloidal gold technology. The test strip is used for solving the problems of complicated operation, dependence on professional instruments and personnel, high detection cost, incapability of meeting the requirement on convenience of early screening and the like in the existing HPV detection technology. Through the method, rapid and accurate detection of the HPV can be realized, and the method is suitable for large-scale early screening including scenes such as home self-inspection and the like.

The invention relates to the technical field of biological detection, in particular to development of a DTT-mediated CRISPR / Cas12a system and HPV (human papillomavirus) detection application. The DTT-mediated CRISPR / Cas12a system is constructed, the PAM-sequence-free nucleic acid is targeted, the nucleic acid detection range of the CRISPR / Cas12a system is remarkably expanded, and meanwhile the analysis sensitivity and specificity of the CRISPR / Cas12a system are improved.

The invention relates to the field of medicine, in particular to a methylation gene detection kit for early detection of cervical cancer, compared with a published cervical cancer methylation marker, the AUC corresponding to cervical cancer lesion detection of the marker can reach 0.986, and when the specificity is 100%, the sensitivity can reach 90.9%. According to the results, the detection sensitivity and the specificity of the kit are greatly improved compared with those of HPV (human papillomavirus) detection. In addition, in an HPV positive sample, the sensitivity and the specificity can be further improved through methylation joint detection. Therefore, the kit can better detect cervical cancer to a great extent, meanwhile, the phenomena of excessive diagnosis and excessive anxiety caused by HPV detection are well solved, and the kit has very high social value.

The application discloses a quality control product for human papillomavirus molecular detection and a preparation method thereof, and belongs to the technical field of biological products. After synthesizing 25 types of HPV full genomes of high-risk types (HPV16, HPV18, HPV26, HPV31, HPV33, HPV35, HPV39, HPV45, HPV51, HPV52, HPV53, HPV56, HPV58, HPV59, HPV66, HPV68, HPV73, HPV82) and low-risk types (HPV6, HPV11, HPV42, HPV43, HPV44, HPV81, HPV83), the HPV full genomes are connected into a pcDNA3.1(+) skeleton vector system, the HPV full genomes are integrated into the genome of a human ovarian cancer cell line ovcar3 respectively, and 25 strains of human ovarian cancer cell lines stably integrating the HPV full genomes are obtained. The new quality control product or kit of various types of simulated clinical samples such as genomic DNA, cell precipitate and cell suspension can be prepared and is suitable for various detection platforms. The human cell line quality control product stably integrating the full-length genomes of the 25 HPV types can more accurately determine the viral load and control the whole process of HPV detection.

The utility model relates to the technical field of HPV (human papillomavirus) detection, and discloses a microwell plate greenhouse culture device for HPV detection, which comprises a lifting handle, a culture box is arranged at the bottom of the lifting handle, and an observation bin is arranged on the side wall of the culture box. Therefore, the culture condition of the culture plate can be observed from multiple angles and in all directions through the transparent observation bin, the problem that the microwell plate for HPV detection is inconvenient to observe in a greenhouse culture device is solved, and the culture plate does not need to be taken out for observation; according to the microwell plate greenhouse culture device for HPV detection, the pull-out plate which can be pulled out and is in sliding fit with the interior of the fixed rail is arranged and then is rotated downwards to be matched with the locking piece of the bottom plate for fixation, so that a plurality of culture boxes can be connected and fixed, batch transportation of the culture boxes is facilitated, and the use convenience of the microwell plate greenhouse culture device for HPV detection is improved.

This invention relates to the field of biological detection, and more particularly to primer combinations, reagent kits, and their applications. The invention provides primer combinations including: primer set I, primer set II, primer set III, primer set IV, primer set V, primer set VI, primer set VII, primer set VIII, primer set IX, primer set X, primer set XI, primer set XII, primer set XIII, primer set XIV, primer set XV, primer set XVI, primer set XVII, primer set XVIII, primer set XIX, primer set XX, primer set XXI, primer set XXII, and primer set XXIII. This invention also provides a reagent kit for rapid HPV detection. This kit can rapidly detect the above 23 HPV types within 20 minutes. The detection limit of the kit is up to 10. 3 The accuracy and specificity are good, with a per-copy / mL ratio.

The invention discloses a preparation method of a kit for detecting human papilloma, and particularly relates to the technical field of gene fragment detection.The kit comprises a micro-fluidic chip, independent reaction units are arranged in the chip in a partitioned mode, and each unit comprises a primer group for specific subtypes of HPV, isothermal amplification enzyme, a CRISPR-Cas12a compound and a quantum dot fluorescent probe; the freeze-drying reagent comprises the primer group wrapped by a freeze-drying protective agent, an isothermal amplification enzyme, a CRISPR-Cas12a (clustered regularly interspaced short palindromic repeats-associated 12a) compound and a quantum dot fluorescent probe. Through cooperation of microfluidic integration, isothermal amplification, CRISPR verification and quantum dot labeling, the bottleneck of traditional HPV detection in sensitivity, specificity and convenience is broken through, meanwhile, the clinical diagnosis value is improved through the double-target design, and the application scene is expanded through the freeze-drying process.

The invention provides a human papilloma virus (HPV) detection kit and a detection method thereof, and the HPV detection method provided by the invention ensures the accuracy and reliability of detection through the steps of systematic sample treatment, PCR amplification, hybridization operation and quality control. The whole detection process is reasonable in design, clear in steps and simple to operate. The whole process from sample treatment to final result interpretation is relatively rapid, especially in PCR amplification and hybridization operation, optimized temperature and time setting is adopted, the detection period is shortened, and the method is suitable for large-scale screening.

The present disclosure provides primers and methods for rapid isothermal amplification of high-risk human papilloma virus.

The invention discloses a sample collection method for HPV detection and an HPV detection method and system, and relates to the technical field of HPV infection detection. The sample collection method comprises the following steps: enabling the sampling unit to be in contact with a to-be-sampled sampling part; then the samples collected by the sampling unit are stored; the sampling part to be sampled is selected from at least one of an oral cavity, a pharyngeal cavity, a laryngeal cavity and a nasal cavity, and the sampling unit is a swab or a cytobrush. The sample collection method provided by the invention has the technical advantages of noninvasiveness, rapidness and relatively low sampling cost, and is convenient for carrying out conventional HPV sampling work in environments such as outpatient service and ward. According to the present invention, the obtained sample is subjected to nucleic acid extraction and nucleic acid detection, such that the HPV infection state can be accurately and efficiently detected, the subtype identification can be accurately and efficiently performed, and the advantages of high detection accuracy and strong specificity are provided.

The invention discloses an HPV (human papillomavirus) detection kit, which solves the problems that the traditional HPV detection needs expensive instruments, is complex to operate and has long detection time by adopting an LAMP (loop-mediated isothermal amplification) technology and a visual detection design. The kit comprises a freeze-drying tube, a liquid reaction tube and a sample cracking tube, wherein an LAMP primer group in the freeze-drying tube is combined with Bst DNA polymerase to realize isothermal amplification; the MOPS buffer microspheres in the liquid reaction tube release components to maintain the reaction environment when the pH changes, the color development accuracy and stability of the neutral red are protected, and the neutral red as an indicator visually presents the result through the color (orange-yellow negative and red positive); during detection, a sample and a lysis solution are mixed and heated and then are added into a reaction system, the constant temperature of 60 DEG C is kept for 30 min, then interpretation can be performed with naked eyes, equipment such as a thermal cycler is not needed, the cost is low, portability is high, and popularization and application of HPV screening in basic medical institutions are effectively promoted.

The invention belongs to the field of molecular biology detection, particularly relates to HPV detection, and more particularly relates to a composition for detecting 26 HPVs and typing the 26 HPVs based on an asymmetric PCR (Polymerase Chain Reaction) single tube. According to the combined detection composition provided by the invention, an asymmetric multiplex PCR analysis method is mainly utilized, and different targets are detected by detecting targets on different pathogens, so that the 26 HPVs are detected and distinguished in a single-tube reaction system at the same time, and a targeted strategy is provided for subsequent treatment. According to the composition, the time required by a traditional detection method is shortened, the whole detection process only needs 40-50 minutes, the timeliness of multiple PCR is improved, and clinical doctors can provide strategies for prevention and treatment of infection caused by the pathogens in a targeted mode.

The invention relates to a non-invasive monitoring and prognosis evaluation method for HPV infection and PIK3CA gene mutation of a head and neck cancer patient based on a multiple digital PCR (Polymerase Chain Reaction) technology. By designing a specific primer probe sequence, high-sensitivity detection of E545K / E542K / E545D mutation of the HPV16 / 33 virus and the PIK3CA gene is realized. According to the detection system, the signal-to-noise ratio is increased by adopting a double-quenching-group probe, the detection of five targets can be synchronously completed in two reaction holes, and the sample consumption is only 20 microliters. The method has the following advantages: (1) the specificity is excellent: wild DNA has no false positive; (2) the limit sensitivity reaches 3 copies; (3) high efficiency and economy: single-sample multi-index detection reduces the detection cost; and (4) the operation is convenient, the matched software automatically calculates the sudden change frequency, and complex signal analysis is not needed. Through comparison and verification with an IPC / FISH gold standard, the HPV detection consistency reaches 90.6%. The method provides reliable technical support for revealing the relevance between the HPV / PIK3CA co-mutation mode and head and neck cancer prognosis.

The invention provides a composition, a reagent and a kit for detecting HPV (human papillomavirus) and application of the composition, the reagent and the kit. The composition comprises more than one of a primer probe group aiming at HPV56, a primer probe group aiming at HPV16 and a primer probe group aiming at HPV18; each primer probe group comprises a probe, a forward primer and 1-5 reverse primers, in each primer probe group, the sequence of the probe can be complementary with a part of sequences of amplification products of the forward primer and the reverse primer, and the forward primer and the reverse primer are primers with the specific sequence provided by the invention. The reagent and the kit comprise the composition. The composition, the reagent and the kit have high accuracy when being used for HPV detection, and have good application prospects in the aspect of detecting whether HPV56, HPV16 and HPV18 exist in a cell sample and a urine sample or not.

The invention discloses a human papilloma virus nucleic acid detection quality control product as well as a preparation method and application thereof. The preparation method comprises the following steps: cloning an HPV whole genome into a vector in two segments to obtain an HPV-A recombinant plasmid and an HPV-B recombinant plasmid; transfecting cells with the two recombinant plasmids and lentivirus plasmids respectively, and packaging to obtain an HPV-A lentivirus and an HPV-B lentivirus; the method comprises the following steps: infecting a host cell with HPV-A lentivirus to obtain an HPV-A cell line; and infecting the HPV-A cell line with HPV-B lentivirus, and screening to obtain a cell line containing HPV-A and HPV-B at the same time, namely the quality control product. According to the preparation method disclosed by the invention, the HPV whole genome lentivirus transfection can be simply and efficiently realized, the background of the prepared quality control product is close to that of a real clinical sample, and the prepared quality control product is high in stability and easy to transport, store and passage; moreover, the system is wide in coverage range, is suitable for multi-purpose research, and is compatible with HPV detection of various platforms.

The application discloses a HPV virus detection kit, which improves the problems of traditional HPV detection, such as the need of expensive instruments, complicated operation and long detection time, by adopting LAMP technology and visual detection design; the kit contains a freeze-drying tube, a liquid reaction tube and a sample lysis tube, LAMP primer groups in the freeze-drying tube are combined with Bst DNA polymerase to realize constant temperature amplification; when the MOPS buffer microspheres in the liquid reaction tube release components to maintain the reaction environment at pH change, the accuracy and stability of the chromogenic accuracy of neutral red are protected, and the neutral red is used as an indicator to intuitively present the result through color (orange yellow negative, red positive); after the sample is mixed with the lysis solution and heated, the reaction system is added at 60 DEG C constant temperature for 30 min, and naked eye judgment can be carried out, without the need of a thermal cycler and other equipment, so that the HPV screening is effectively promoted in the popularization and application of primary medical institutions.

The utility model discloses a sampling brush for HPV (human papillomavirus) detection. The sampling brush comprises a sampling rod, a limiting seat is connected to the side end of the sampling rod, a clamping seat is arranged on one side of the limiting seat, a brush head is fixed to the side end of the clamping seat, sampling bristles are arranged at the end of the brush head, side bristles are arranged at the side end of the brush head, rapid sampling is conducted through the sampling bristles and the side bristles, and the problems of insufficient sampling and damage caused when rotation is needed are solved; a cavity is formed in the inner side of the sampling rod, a moving assembly is arranged in the cavity, and a clamping assembly is arranged in the limiting seat. According to the sampling brush for HPV detection, the sampling rod and the brush head are used for sampling operation, the problem that the accuracy is low due to the fact that the depth and the direction cannot be controlled during operation is solved, the sampling range is increased due to the arranged sampling bristles and side bristles, and the problem that inconvenience is caused due to the fact that repeated rotation sampling is needed is solved; and the damage to the tissue structure of the inner wall of the cervix uteri is reduced.

The invention provides an intelligent HPV (human papillomavirus) typing detection system and method, and relates to the technical field of HPV detection. The intelligent HPV typing detection system comprises a hardware assembly and a software module, the hardware assembly comprises a sample collection tool, a PCR amplification instrument, a chip scanner, a computer and auxiliary equipment, and the software module comprises an image analysis module, a data processing module, a result report generation module and a system control module. By utilizing artificial intelligence and an image recognition technology, an HPV gene chip image can be accurately analyzed, and a system can learn a large number of HPV positive and negative sample image features through a deep learning algorithm, so that a positive region in the chip image can be accurately recognized, fine features in the image can be automatically recognized, and the detection accuracy is improved. The subjectivity and errors of manual judgment are avoided, and the detection accuracy is greatly improved.