Typing method for human papilloma virus gene

Abstract

The invention discloses a genotyping method of human papilloma virus, which carries out permutation and combination on the sequences of 25bp behind GP5 of 40 common HPV subtypes by designed software, and the detailed steps are as follows: 1. the sequences of 25bp behind GP5 of a single type of the 40 common HPV subtypes; 2. mixed sequence mode of 25bp behind GP5 after arbitrary two types of the 40 common HPV subtypes are combined; 3. mixed sequence mode of 25bp behind GP5 after arbitrary three types of the 40 common HPV subtypes are combined; 4. mixed sequence mode of 25bp behind GP5 after arbitrary four types of the 40 common HPV subtypes are combined; 5. mixed sequence mode of 25bp behind GP5 after arbitrary five types of the 40 common HPV subtypes are combined; and 6. mixed sequence mode of 25bp behind GP5 after arbitrary six types of the 40 common HPV subtypes are combined. All the modes carry out pairing with the bases following GP5 by the ATCG sequence, and the pairing number is recorded, circulation is carried out in this way until the sequences of 25bp behind GP5 are all paired, then a pyrosequencing mode database infected by 1 to 6 types of different subtypes is created, the sample is carried out DNA extraction and MY09 / 11 amplification, the amplification product is taken as a template and then carried out amplification by GP5+ / 6+, then GP5 is taken as a sequencing primer, and the sequencing result is compared with the pyrosequencing mode database to determine the type. The genotyping method has the advantages of low cost, convenient operation, strong specificity and synchronously detecting a plurality of types of inflection.

Description

technical field [0001] The invention relates to a human papilloma virus genotyping method, which belongs to the technical field of medical genetic diagnosis. Background of the invention [0002] Cervical cancer is one of the common gynecological malignancies. According to worldwide statistics, its incidence ranks second among female malignant tumors, second only to breast cancer. There are about 493,000 new cases of cervical cancer every year in the world, and 274,000 women die from the disease, 83% of which occur in developing countries, accounting for 15% of female tumors in developing countries, while cervical cancer in developed countries It accounts for only 3.6% of tumors in women. There are 131,500 new cases of cervical cancer in my country every year, accounting for a quarter of the new cases of cervical cancer in the world. After decades of extensive gynecological census, the incidence and mortality of cervical cancer have been reduced by nearly 68%. However, fro...

AI Technical Summary

Problems solved by technology

The second-generation hybrid capture (hubrid capture II, HC-II) test is so far the only HPV detection method that has been certified by the US Food and Drug Administration (FDA) for clinical use. For HPV genotyping, high-risk and low-risk types cannot be detected at the same time, and multiple infections cannot be judged

The type-specific hybridization method has high sensitivity, can detect multiple PCR products at one time, and has a high ability to detect multiple infections, but its disadvantage is that it can only be used to detect certain known common types, and it is not suitable for unknown types and variant types. HPV cannot be detected, and the inherent problem of hybridization cannot be ruled out, that is, the possibility of cross-hybridization

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