50results about How to "Wide detection coverage" patented technology

The invention discloses a human leukocyte antigen HLA-A and HLA-B gene full-length sequencing method and an HLA gene sequencing and typing method. The HLA-A and HLA-B gene full-length sequencing method comprises the following steps of: a, performing PCR amplification on about 4kb full-length sequences of HLA-A and HLA-B genes by using a pair of primers respectively; and b, cloning the amplification products to a pGEM-Tea sy vector, sequencing the full-length sequences by using ten walking sequencing primers in positive and negative directions respectively, and totally obtaining 38 allele 4.3kb full-length sequences of the HLA-A and 30 allele 3.7kb full-length sequences of the HLA-B. The HLA-A and HLA-B sequencing and typing method comprises the following steps of: performing PCR amplification on typing target areas of the HLA-A and HLA-B by using two pairs of primers respectively; and performing two directional sequencing on products by using fourteen sequencing primers respectively, wherein the HLA-DRB1 and HLA-DQB1 sequencing and typing method comprises the following steps of: amplifying sequences of second and third exons of DRB1 and DQB1 by adopting group specificity primers respectively; performing two directional sequencing on the second and third exons of the DRB1 by adopting eight group specificity primers and three sequencing primers; and performing two directional sequencing on the second and third exons of the DQB1 by adopting four sequencing primers respectively.

The present invention provides non-small cell lung cancer markers and the use thereof. The non-small cell lung cancer markers in the present invention include at least one of the 26 selected detectable mature microRNAs existing stably in human serum or plasma. The invention also provides a probe combination, kit and biochip for detecting the non-small cell lung cancer markers. The invention further provides a method for detecting microRNAs in the serum of lung cancer patients. By detecting the variations of microRNAs in the serum of lung cancer patients, the disease can be diagnosed in vitro; the progression course of the disease can be predicted; the occurrence of complications, the rate of relapse and the prognosis of the disease can be monitored; the drug efficacy and therapeutic effects can be analyzed. The method in the present invention enables extensive detection spectrum, high sensitivity, low cost, convenient sample taking and preservation. The method can be applied in the general survey of disease, solves problems of the low specificity and sensitivity encountered with previous single markers, and increases significantly the clinical detection rate of diseases, all of which make it an effective means for diagnosing diseases at an early stage.

The invention provides an ellipticity measuring instrument for a tube body and a measurement method thereof. The measuring instrument comprises a rigid semi-sphere, a contact end and a scale micrometer, wherein the contact end and the scale micrometer are arranged at two semicircular ends of the rigid semi-sphere respectively; the measurement directional line of the scale micrometer is on the same straight line as the contact end, and the straight line passes through the center of the rigid semi-sphere; the diameter of the rigid semi-sphere is equal to the sum of the distance between the contact end and the rigid semi-sphere, the distance between a measurement contact of the scale micrometer and the rigid semi-sphere, and the diameter of a tested tube body. The ellipticity measuring instrument is convenient to use, simple in structure, high in precision and measurement efficiency, low in work intensity and wide in detection coverage, can cover the ellipticities of all steel tubes with various specifications and calibers and solves the problem of ellipticity measurement of the tube body.

The present invention provides microRNAs for assessing the status of pancreatic cancer in a subject, and provides methods, kits, and biochips for detecting said microRNAs.

The invention provides a carrier communication module detection device and a carrier communication module detection method. The technical problem that the overall detection cannot be realized in the prior art is solved. The device comprises a multi-way network test cabinet. The multi-way network test cabinet is connected with a concentrator communication cabinet. The concentrator communication cabinet comprises a concentrator. The concentrator communication cabinet is connected with a plurality of carrier meter communication cabinets which are connected in sequence. The carrier meter communication cabinets are connected with a carrier meter set. The carrier meter set comprises a plurality of carrier meters which are connected in series in sequence. The concentrator communication cabinet and the carrier meter communication cabinets are respectively connected with respective spectral instruments. Each carrier meter communication cabinet comprises a second attenuator. The second attenuator in a next carrier meter communication cabinet is connected with a previous carrier meter communication cabinet. The second attenuator in the front-most carrier table communication cabinet is connected with the concentrator communication cabinet. According to the invention, to-be-tested carrier communication meter reading equipment is arranged in the whole system, so that the overall use effect of the carrier communication meter reading equipment can be detected.

The invention relates to a comprehensive self-checking method of an automatic tester. The tester comprises an insulation and voltage resistance tester, a plurality of switching boxes, a main control module and a universal meter measurement module, wherein the main control module and the universal meter measurement module run on an industrial personal computer; a function switching module and a test point switching module connected with a tested point run in each switching box; and the main control module sends instructions to the function switching modules, the function switching modules analyze the instructions and send the instructions to the test point switching modules, and the function switching modules and the test point switching modules control on-off of related relays according to the instructions to realize self-inspection, and the self-inspection comprises conduction self-inspection, short circuit self-inspection, insulation self-inspection, anti-electricity self-inspection, switching box self-inspection and relay residual life self-inspection. According to the comprehensive self-checking method of the automatic tester, the detection coverage is wide, the accuracy of subsequent testing is ensured, and a guarantee is provided for normal use of equipment.

The invention discloses a primer, a kit and a method related to determination of full-length sequence of human leukocyte antigen HLA-E, -F and-G genes. By adopting the primer and the method, whole HLA-E, -F and-G genes are amplified with clear bands, specific amplification, and good quality of peaks of sequencing results. The whole HLA-E, -F, -G gene sequences and typing results can be obtained bysplicing the sequences detected by walking primers.

The invention discloses a WEB vulnerability detection method based on passive traffic, relates to the technical field of vulnerability detection, and aims to improve the scanning test efficiency of WEB vulnerability. The method comprises the following steps: receiving a message to be tested; analyzing the message, and storing the analyzed message; scanning the parsed message, including performinggeneral vulnerability scanning, POC integrated scanning and port scanning on the parsed message to obtain a vulnerability detection result, and storing the vulnerability detection result; and returning the vulnerability detection result. The invention further discloses a WEB vulnerability detection device based on the passive traffic, electronic equipment and a computer storage medium.

The invention discloses a method and a device for detecting a dead zone of an unmanned vehicle. The unmanned vehicle is connected with one end of a telescopic cross bar through a pivot; the other end of the cross bar is connected with a telescopic longitudinal bar; and the cross bar rotates around the pivot to drive the longitudinal bar to move. A specific implementation mode of the method comprises the following steps: receiving a starting detection request, wherein the starting detection request comprises the position of the longitudinal bar, the length of the longitudinal bar and the set moving speed of the longitudinal bar; detecting the longitudinal bar; determining an expected detection time according to the position and the set moving speed of the longitudinal bar; recording the actual detection time in response to the detected longitudinal bar; determining the omitted detection position of the longitudinal bar according to a time difference between the actual detection time and the expected detection time in response to the determination that the actual detection time is inconsistent with the expected detection time; and determining the dead zone according to the length and the omitted detection position of the longitudinal bar. The implementation mode can quickly and accurately determine the dead zone of the unmanned vehicle.

The invention relates to an ultra-high performance liquid chromatography-high-resolution quadrupole flight time mass spectrometry method for extracting lipids in freeze-dried food based on an ultrasonic single solvent, which is characterized in that the lipids in the freeze-dried food are extracted by using an ultrasonic-assisted single solvent, and then positive and negative ions are quickly scanned by using ultra-high performance liquid chromatography-high-resolution quadrupole flight time mass spectrometry within 34 min to realize rapid qualitative and quantitative analysis of the lipids. The method is fast and simple to operate, the extraction is not affected by protein, the extraction time is short, the toxicity of the solvent is small, the repeatability is good, the recovery rate ofvarious lipids is high, and the purpose of high coverage of the lipids is achieved.

The invention relates to the technical field of biological detection, in particular to a leukemia B cell minimal residual detection method. According to the invention, 10 upstream primer sequences are designed directed at a variable V region of human PCR, 1 downstream primer sequence is designed respectively directed at A, D, M, G gene constant regions of BCR, 2 downstream primer sequences are designed directed at an E gene constant region of BCR, and multiplex PCR is carried out to amplify a target sequence, after purification of the target sequence, a basic group A is added to the 3' end of the product sequence and the product is connected to an adapter sequence, the connection product containing the adapter is subjected to PCR amplification with sequencing primers so as to obtain an amplification product, and then purification and sequencing are carried out, and the sequencing result is compared with a cancer cell RNA amplification product sequence. The method has sensitivity up to 10<-6>, and wide detection coverage, and can detect almost all B cell receptors.

The invention discloses a kit and a detection method for rapidly detecting multiple virulence factors GeXP of vibrio harveyi. A genome DNA (deoxyribonucleic acid) of the vibrio harveyi (V. Harveyi) is firstly extracted to serve as a template, twelve pairs of polygene PCR (polymerase chain reaction) primers and a pair of universal primers are used for multiple PCR amplification to obtain multiple PCR reaction products, and the products are detected and analyzed by a Genome Lab GeXP genetic analysis system. Specific primers are designed according to virulence factors of twelve known types of vibrio harveyi, twelve genes are rapidly and efficiently detected once by means of single-tube reaction to know whether detected samples contain the vibrio harveyi carrying the virulence factors and having potential pathogenicity or not, detection coverage is wide, accuracy of evaluating pathogenicity of the vibrio harveyi can be greatly improved, more effective detection measures are provided for evaluating pathogenicity of the vibrio harveyi, and the detection method has important application prospects in aquaculture disease detection, epidemiological investigation, detection of pathogenic bacteria in food, aquatic product import and export detection, hospital infection monitoring and the like.

The invention discloses a communication link layer message single event effect fault tolerance method and device. The method comprises the steps of adding a message real-time read-back channel in an FPGA link layer, and comparing a real-time read-back message with original application data; and after data errors are judged,realizing fault-tolerant processing of the single event effect in the message transmission process of the communication link layer based on two error processing methods. The method provided by the invention does not depend on an external processor, can avoid the serious problems of protection maloperation and the like caused by SV and GOOSE message error output due to the influence of a single event effect on the FPGA, and greatly improves the reliability of the secondary equipment of a power system.