52 results about "Hla genes" patented technology

The invention discloses a human leukocyte antigen HLA-A and HLA-B gene full-length sequencing method and an HLA gene sequencing and typing method. The HLA-A and HLA-B gene full-length sequencing method comprises the following steps of: a, performing PCR amplification on about 4kb full-length sequences of HLA-A and HLA-B genes by using a pair of primers respectively; and b, cloning the amplification products to a pGEM-Tea sy vector, sequencing the full-length sequences by using ten walking sequencing primers in positive and negative directions respectively, and totally obtaining 38 allele 4.3kb full-length sequences of the HLA-A and 30 allele 3.7kb full-length sequences of the HLA-B. The HLA-A and HLA-B sequencing and typing method comprises the following steps of: performing PCR amplification on typing target areas of the HLA-A and HLA-B by using two pairs of primers respectively; and performing two directional sequencing on products by using fourteen sequencing primers respectively, wherein the HLA-DRB1 and HLA-DQB1 sequencing and typing method comprises the following steps of: amplifying sequences of second and third exons of DRB1 and DQB1 by adopting group specificity primers respectively; performing two directional sequencing on the second and third exons of the DRB1 by adopting eight group specificity primers and three sequencing primers; and performing two directional sequencing on the second and third exons of the DQB1 by adopting four sequencing primers respectively.

The invention provides methods and processes for the identification of polymorphisms at one or more designated sites, without interference from non-designated sites located within proximity of such designated sites. Probes are provided capable of interrogation of such designated sites in order to determine the composition of each such designated site. By the methods of this invention, one or more mutations within the CFTR gene and the HLA gene complex can be can be identified.

A method for detecting one or more HLA sequence types is described that comprises the steps of: amplifying a plurality of first amplicons from a double stranded nucleic acid sample, wherein the first amplicons are amplified with a plurality of pairs of nucleic acid primers that define exons 2 and 3 of both strands of HLA loci from the group consisting of HLA-A, HLA-B, and HLA-C; amplifying the first amplicons to produce a plurality of populations of second amplicons, wherein each population of second amplicons is clonally amplified from one of the first amplicons; sequencing the plurality of populations of second amplicons to generate a nucleic acid sequence composition for each of the plurality of second amplicons; and detecting variation in the sequence composition from one or more of the second amplicons for one or more of the HLA loci.

The invention provides a method for amplifying an HLA gene and a specificity primer pair used by the method and also provides a method for typing the HLA gene and an amplifying primer pair and a sequencing primer pair which are used by the method.

The application of induced pluripotent stem cells (iPSCs) to generate adoptive cell therapy products and usage of iPSCs for screening potential toxicity of immune receptors are described herein. In some aspects, iPSCs and cells differentiated therefrom are provide which do not express a HLA gene (e.g., HLA-A).

The invention discloses an HLA (human leukocyte antigen) gene specific PCR (polymerase chain reaction) amplification primer, an HLA typing method and an HLA typing kit. A sequence of the PCR amplification primer comprises a primer sequences shown as SEQ ID No. (sequence identification number) 1-2, 3-4, 5-6, 7-8 and 9-10. The HLA gene typing method comprises the steps that the HLA gene specific PCR amplification primer is used for conducting PCR amplification and purification on sample DNA (deoxyribonucleic acid); a gene exon sequencing primer is used for conducting sequencing PCR amplification, purification and sequencing on an HLA gene amplification product, the sequence of the HLA gene amplification product is compared with a standard sequence; and the type of the HLA gene is determined. The HLA gene typing kit comprises the PCR amplification primer and the sequencing primer. A qualitative leap in aspects of detection flux, data quality, cost control and the like is achieved; data is more reliable and realer; and the problem that typing cannot be conducted when nucleotide of certain alleles is positioned outside an amplification area is solved.

The invention provides an HLA (Human Leukocyte Antigen) gene high-resolution genotyping method based on an Illumina GA sequencing technology and a primer label for the method.

The invention provides an HLA (Human Leukocyte Antigen)-A,B genotyping PCR (Polymerase Chain Reaction) primer, and a method of applying the PCR primer to HLA gene analysis based on a sequencing method.

The invention discloses a parting method of leucocyte antigen gene of human being, comprising the following steps of: (1) extracting genome DNA to be tested by a regular technology, and amplifying a destination gene fragment to be analyzed by using PCR amplification primer: 2,3,4 exon of HLA-A, 2,3,4 exon of HLA-B and exon on the locus 2 of HLA-DRB; and (2) amplifying the PCR output obtained in the step (1) by using sequencing primer, amplifying the exon, sequencing the amplified exon and comparing the sequencing result with the standard sequence in a database to determine the gene parting result. As the 2,3,4 exon of HLA-A, 2,3,4 exon of HLA-B and exon on the locus 2 of HLA-DRB are effectively amplified as a result of optimized combination of the HLA gene sequencing kit and the test condition, and the corresponding exon is sequenced, the invention solves the problem that effective parting can not be performed when certain allelic gene nucleotide is located outside an amplification area during further parting, thereby improving the parting resolution and accuracy of the HLA gene.

Methods are provided to determine the genomic sequence of the alleles at the HLA gene. The resultant sequences provide linkage information between different exons, and produces the unique sequence at each gene from the two alleles of the individual sample being typed. The sequence information provides an accurate HLA haplotype. Methods to decrease allele dropout during long range PCR reactions are also disclosed.

This invention provides for an improved method for genotyping HLA loci using PCR.

The invention relates to biomedicines and immunological genetics and particularly relates to a group-specific amplification primer, sequence-based typing method and a kit for HLA genes. Sequences of a PCR amplification primer are selected from sequences represented by SEQ ID NO.1-28, sequences represented by SEQ ID NO.29-64 and sequences represented by SEQ ID NO.65-94. By designing the group-specific amplification primer by carrying out serum classification on the HLA genes, all allelic genes including HLA-A, -B and -C can be subjected to sequence-based typing; by carrying out amplification sequence on full-length sequences of genes HLA-A, -B and -C, the ambiguity can be effectively solved, an absolute high score is achieved, and data is relatively accurate and reliable.

Provided herein are methods for accurately determining the alleles present at a locus that is broadly applicable to any locus, including highly polymorphic loci such as HLA loci, BGA loci and HV loci. Embodiments of the disclosed methods are useful in a wide range of applications, including, for example, organ transplantation, personalized medicine, diagnostics, forensics and anthropology.

The invention relates to the field of biotechnology, and particularly provides a method for targeted capture and sequencing of HLA gene sequences. The method comprises the following steps: 1) hybridizing a denatured nucleic acid sample with a nucleic acid probe library fixed on a solid-phase carrier under a hybridization condition to form a target nucleic acid molecule-probe-solid-phase carrier membrane complex; 2) cleaning the 'probe-solid-phase carrier membrane complex' bound with target nucleic acid in step 1) by using a cleaning solution, eluting the probe-solid-phase carrier membrane complex bound with the target nucleic acid from a solid-phase carrier membrane by using an eluting solution, purifying, enriching or constructing to obtain a nucleic acid library; and 3) taking the nucleic acid library obtained by enrichment or constructed after enrichment in step 2) for high-throughput sequencing. A nucleic acid probe which can rapidly generate high-fold coverage and single-base displacement and is used for enriching HLA genes can be immovably fixed on the solid-phase carrier membrane. The method has characteristics of simplicity and convenience in operation, flexible probe acquisition and low cost, is favorable for improving the HLA genotype identification accuracy, and is rapid and convenient.

The invention is a method of detecting or assessing solid organ graft (transplant) rejection by detecting donor-specific HLA alleles in a blood sample of a graft (transplant) recipient. The invention further comprises a method of detecting the presence of maternal cells in a blood sample of an offspring.

The invention discloses a method and device for HLA genotyping, a storage medium and a processor. The method for HLA genotyping includes the steps: constructing an HLA genotype database, wherein the HLA genotype database comprises a DNA sequence of pseudogenes associated with HLA-A genes; comparing the sequencing data of the sample to be tested with the HLA genotype database, and retaining the genotype satisfying the predetermined condition as a comparison result; and selecting the genotype with the highest comparison value in the comparison result as the result of HLA genotyping of the sampleto be tested. For the method for HLA genotyping, adding an HLA pseudogene sequence very similar to the HLA gene sequence in the HLA genotyping database helps to reduce the false positive results of typing, thus solving the technical problem of high false positive HLA genotyping in the prior art.

The purpose is to provide a high-precision DNA typing method and kit that make full use of a high-throughput sequencer and eliminate the ambiguity derived from phase ambiguity. The invention provides an HLA DNA typing method characterized by including: (1) a step for providing a set of primers that each hybridize specifically to an upstream region and a downstream region of at least two genes selected from genes belonging to HLA class I and class II in the nucleotide sequence of the human genome and amplify under identical PCR conditions; (2) a step for amplifying these at least two genes in a test sample (DNA) simultaneously in the same container under identical PCR conditions using this set of primers; (3) a step for determining the nucleotide sequence of the PCR product; and (4) an optional step for conducting a homology search of a database.

The present invention relates to a method for producing immune-compatible cells or a cell population which comprises a step of editing one or two alleles of one or more immune-compatible antigen genes by gene deletion or modification in an isolated cell comprising at least one of the immune-compatible antigen genes selected from HLA (human leukocyte antigen)-A, HLA-B and HLA-DR, to immune-compatible cells produced by the method, and to a cell population comprising the immune-compatible cells produced by the method

Methods of assessing the risk of clinical signs of hypersensitivity reaction to nucleoside antiviral compounds, including abacavir, are described. The methods include genotyping subjects for polymorphisms in the TNFα gene, the class 1 HLA genes, or a combination of both the TNFα and HLA genes.

The present invention addresses the problem of providing a method and kit for the DNA profiling of HLA genes using a high-throughput massively parallel sequencer. The present invention pertains to a method for the DNA profiling of HLA genes, said method being characterized by including: (1) a step for preparing a primer set that anneals specifically to exon 4 and intron 1 and includes exon 2 and exon 3 of at least one target gene selected from the group consisting of HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5, HLA-DQB1 and HLA-DPB1 in the base sequence of the human genome; (2) a step for amplifying a sample (DNA) by PCR using the primer set; (3) a step for determining the base sequence of the amplified PCR product; and (4) a step for carrying out a homology search against a database.

The invention belongs to the fields of genomics and bioinformatics, relates to an HLA genotype-SNP linkage database, a construction method thereof, and a HLA typing method. Specifically, the construction method of the HLA genotype-SNP linkage database comprises the following steps: a) selecting one or more HLA loca sequences as a reference sequence; b) comparing the known type HLA genes in a conventional HLA database with the reference sequence to find out a difference site of the reference sequence i.e. a SNP site, and to obtain an SNP linkage relationship relative to the reference sequence for constructing the HLA genotype-SNP linkage database. The present invention also relates to a method for determining SNP linkage relationship of HLA gene, and an HLA typing device. The method of the present invention achieves low-cost, high-throughput, high-accuracy and high-resolution typing for HLA.

The invention provides a primer group, a kit and a method for HLA (human leukocyte antigen) gene amplification and gene parting and belongs to the field of gene detection. According to the primer group for HLA gene amplification, primers are designed according to 8 exon zones of an HLA-A gene, 8 exon zones of an HLA-B gene and an exon 2 and an exon 3 of an HLA-DRB1 gene, PCR amplification is performed on the HLA gene by use of the primers, and a product with a gene segment length larger than 400 bp and smaller than 1.5 kb is obtained. By means of the PCR amplified HLA genes, the gene segment length of the amplification product is larger than 400 bp and smaller than 1.5 kb, the requirement for completeness of a template is reduced, the amplification efficiency is high, the amplification reaction time is short, the cost is reduced, and the demands of large-scale amplification or gene parting of sample HLA genes can be met.

The invention provides a kit for detecting lung cancer susceptibility genes. The kit comprises a primer aiming at a single nucleotide polymorphism site rs4488809 (T-C) on a TP63 gene, a primer aiming at a single nucleotide polymorphism site rs36600 (T-C) on an MTMR3 gene, and a primer aiming at a single nucleotide polymorphism site rs2395185 (G-T) on an HLA gene.

The invention belongs to the technical field of gene engineering, and discloses a primer combination for high-resolution typing of HLA gene, a kit containing the primer combination, and a method for high-resolution typing of HLA gene. Compared to the traditional methods such as SBT, SSO and the like, the method of the present invention has the following advantages that the sequencing reaction cansimultaneously determine 480 samples so as to provide high-throughput characteristic, the HLA typing results obtained by using the method of the present invention contain the low-resolution typing results and the high-resolution typing results so as to provide high-resolution characteristic, and the HLA typing detection results of 3000 random samples show that the typing accuracy can achieve 100%so as to provide high accuracy characteristic.