Group-specific amplification primers, sequencing typing method and kit for hla gene

A technology of group-specific amplification and amplification primers, applied in the fields of biomedicine and immunogenetics

Inactive Publication Date: 2019-01-29
SHENZHEN GENEBIOHEALTH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention is dedicated to solving the above-mentioned three major shortcomings in the current HLA high-resolution sequencing and typing technology at home and abroad, solving the defects of existing HLA sequencing and typing products, and achieving the absolute high score of HLA of the tested specimen. The present invention provides a Group-specific amplification primers, sequencing and typing methods and kits for HLA genes

Method used

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  • Group-specific amplification primers, sequencing typing method and kit for hla gene
  • Group-specific amplification primers, sequencing typing method and kit for hla gene
  • Group-specific amplification primers, sequencing typing method and kit for hla gene

Examples

Experimental program
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Effect test

Embodiment 1

[0102] This example gives an example of HLA-A, -B, -C absolute high-resolution sequencing typing of 300 cases of Shenzhen patients requiring hematopoietic stem cell transplantation and their donor samples.

[0103] Selected 300 samples of known HLA-A, -B, -C serotypes, and carried out sequencing typing with the corresponding group-specific PCR amplification primers and sequencing primers of the present invention to verify the implementation effect of the present invention .

[0104] For each sample, first, according to its HLA serology, select corresponding group-specific PCR amplification primers as described in the first aspect of the application to perform PCR on the full-length sequences of HLA-A, -B, and -C of the sample Amplification reaction, amplification reaction conditions: All amplification reactions are set with the same reaction system, the composition is as follows:

[0105]

[0106] After the PCR reaction system is set up, all reactions are amplified synchrono...

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Abstract

The present invention provides a group-specific amplification primer, sequence-based typing method, and kit for HLA genes. A sequence of the PCR amplification primer is selected from a sequence represented by SEQ ID NO.1-28, a sequence represented by SEQ ID NO.29-64, and a sequence represented by SEQ ID NO.65-94. By means of the present invention, a group-specific amplification primer is designed by performing serum classification on HLA genes, so as to perform sequence-based typing on all allelic genes of HLA-A, -B, and -C, and perform amplification sequencing on full-length sequences of HLA-A, -B, and -C.

Description

technical field [0001] The invention relates to biomedicine and immunogenetics, in particular to a group-specific amplification primer for HLA genes, a sequencing typing method and a kit. Background technique [0002] Human leukocyte antigen system (HLA) is the most diverse and important immunogenetic system in the human genome. Its diversity determines the body's resistance to infectious diseases, autoimmune diseases, genetic diseases and various tumors. In clinical application, the matching degree of its gene polymorphism between donor and recipient directly determines the survival rate of hematopoietic stem cell transplantation and organ transplantation patients. [0003] In the field of HLA tissue typing industry, there are currently three major shortcomings in the products in the domestic and foreign markets: First, most of the products only type for some exons of HLA genes, such as only for HLA-I genes (including The exons 2, 3, and 4 of HLA-A, -B, and -C) are tested ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6858C12Q1/6869
CPCC12N15/11C12Q1/68
Inventor 于浩洋徐筠娉马塞尔·G·J·蒂拉努斯
Owner SHENZHEN GENEBIOHEALTH
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