17 results about "Group specific primers" patented technology

The invention belongs to the technical field of plant fungus molecular biology detection, and discloses an LAMP primer group, a kit and a detection method for detecting oat smut pathogenic bacteria. A group of LAMP (loop-mediated isothermal amplification) specific primers are designed according to a specific sequence on a whole genome of the oat smut pathogenic bacteria Ustilago hordei, results are judged through a real-time fluorescence quantification method, an agarose gel electrophoresis method and an SYBR Green I fluorescent dye developing method, and the oat smut pathogenic bacteria carried by oat seeds are detected. The LAMP detection method for the oat smut pathogenic bacteria, established by the invention, is strong in specificity, high in sensitivity, high in speed and low in cost, provides a new technical means for detection of the oat smut pathogenic bacteria carried by the oat seeds, and has relatively high practical application value.

The invention discloses a primer group and method for human metapneumovirus whole genome sequencing and application. Two groups of specific primer pools are designed for the metapneumovirus, totally comprise 21 primers, can effectively amplify the metapneumovirus type A and the metapneumovirus type B, are high in sensitivity and suitable for multi-platform detection, and provide a new direction for detection, typing, traceability and evolutionary analysis of the metapneumovirus.

The invention belongs to the technical field of molecular breeding of crops, and discloses a KASP molecular marker related to the size of soybean seeds and application of the KASP molecular marker. The molecular marker is developed on the basis of a GmW82.09G173900 gene in a soybean genome assembled by T2T, and targets four SNP (Single Nucleotide Polymorphism) sites which are obviously associated with the size (covering hundred-grain weight, seed length, width, area and perimeter) of a soybean seed in a downstream regulation and control region of the gene, and the physical positions are respectively 42109039bp (SNP1), 42109150bp (SNP2), 42109166bp (SNP3) and 42110442bp (SNP4). The invention provides three groups of specific KASP primer combinations and a detection method, through PCR amplification and fluorescence signal detection, soybean genotypes can be rapidly distinguished, accurate screening of large soybean individuals is realized, and an efficient technical tool is provided for accurate improvement of soybean seed size characters.

The invention relates to the technical field of molecular breeding of crops, in particular to a KASP molecular marker related to the size of soybean seeds and application of the KASP molecular marker. The molecular marker group comprises three SNP (Single Nucleotide Polymorphism) sites located in an intron region of a gene GmW82.04G097200 of a soybean T2T genome, wherein an SNP1 site is located at 8152017bp, and T / C nucleotide polymorphism exists; the SNP2 site is located at 8152443bp, and the A / G nucleotide polymorphism exists; the SNP3 site is located at 8153901bp, and C / G nucleotide polymorphism exists in the SNP3 site; alleles of the three SNP sites are combined to form three haplotypes corresponding to soybean seeds in size. The method has the advantages that three groups of specific KASP primer groups are provided, and genotypes of different soybeans are rapidly distinguished by virtue of PCR amplification and fluorescence signal detection, so that accurate screening of large-grain phenotype individuals is realized, the breeding period is effectively shortened, and the breeding input cost is reduced.

The invention discloses a kit for detecting intestinal core bacteria by a TaqMan real-time fluorescent quantitative PCR (Polymerase Chain Reaction) method, and belongs to the technical field of kits. The kit disclosed by the invention comprises standard positive templates of nine common intestinal core bacteria and nine groups of specific primers and probes which are required in a PCR amplification process and correspond to the standard positive templates. The kit disclosed by the invention is simple, convenient and rapid to operate; the specificity is good and the sensitivity is high; complicated raw information data analysis does not need to be carried out; the intestinal core bacteria can be detected and quantified without high-throughput sequencing, and three bacteria can be detected in one reaction hole, so that the cost is greatly reduced. The kit provided by the invention can be used for rapidly and quantitatively detecting nine intestinal core bacteria including bifidobacterium, lactobacillus, prevotella, bacteroides and the like, can partially replace a high-throughput detection method which is higher in cost and more complicated to operate according to research and diagnosis purposes, and is suitable for being widely popularized in clinical detection and laboratory research.

The invention discloses a Ganoderma sinensis strain and a molecular marker identification method thereof, and relates to the technical field of microorganisms, the Ganoderma sinensis strain is preserved in China Center for Type Culture Collection, and the preservation number is CCT CC NO: M 20251554; the ITS sequence of the Ganoderma sinensis strain is as shown in SEQ ID NO: 15; according to the method, ganoderma foense 37 is successfully bred through a cross breeding technology, the strain has the comprehensive excellent characters that the yield is high, the polysaccharide content is remarkably increased, and the taste is close to that of wild ganoderma foense, the problems that an existing cultivated variety is reduced in active ingredient and poor in taste are solved, meanwhile, a set of molecular marker identification method based on seven groups of specific primers is established, and the method is suitable for large-scale popularization and application. The method can be used for rapidly, accurately and reliably identifying the ganoderma fosinense 37 and effectively distinguishing the ganoderma fosinense 37 from parents and other strains, and is simple and convenient to operate, good in repeatability and suitable for wide popularization and application.

The invention discloses an HLA-DRB1 gene high-resolution typing method based on group specific primers, and belongs to the technical field of gene detection. The group of specific primers are respectively designed according to exons 1-4 of the HLA-DRB1 gene, sequences of the specific primers are shown as SEQ ID NO.1-SEQ ID NO.20, the specific primers have good conservative property on allele sequences of the HLA-DRB1 gene, compared with gene sequences of HLA-DRB3, HLA-DRB4 and HLA-DRB5, the specific primers have good specificity, 1, 2, 3 and 4 exons of the HLA-DRB1 site can be effectively amplified, the corresponding exons are sequenced, and the specific primers can be used for detecting the HLA-DRB1 gene. The polymorphic site of the HLA-DRB1 gene is fully covered. Therefore, when further typing is carried out, the resolution and accuracy of HLA-DRB1 genetic typing are effectively improved, and the ambiguous condition of HLA-DRB1 genetic typing is improved.

The invention discloses a monkey pox virus typing nucleic acid detection primer probe combination, a kit and application, and belongs to the technical field of biology. The primer probe combination comprises six groups of specific primer pairs and six corresponding probes, and the six groups of specific primer pairs and the six corresponding probes are respectively used for accurately identifying the monkey pox viruses such as Clade Ia, Clade Ib, Clade II, Clade IIb A.1, Clade IIb B.1 and Clade IIb C.1; the primer probe combination provided by the invention has the advantages of high specificity, high sensitivity and accurate typing, and effectively overcomes the influence of target sequence deletion or mutation caused by virus variation on the detection accuracy through combination of locked nucleic acid modified probes and a multiplex fluorescent PCR technology; the method can realize rapid and parallel identification of main branches and epidemic pedigree of the monkey pox virus, and provides reliable technical support for epidemic prevention and control and clinical diagnosis.

The invention relates to the technical field of gene detection, aims to solve the problem of incomplete transposon detection of current AvrPiz-t, and particularly provides a primer and a kit for rapidly identifying a transposon-inserted toxic strain in a magnaporthe oryzae avirulence gene AvrPiz-t and application of the primer and the kit for rapidly identifying the transposon-inserted toxic strain in the magnaporthe oryzae avirulence gene AvrPiz-t. The invention develops a group of specific primers for the first time, and the primers can quickly and comprehensively detect whether transposon fragments are inserted into a non-coding region and a coding region of the magnaporthe oryzae AvrPis-t gene or not. The primer can be used for rapidly detecting whether the transposon fragment is inserted into the magnaporthe oryzae AvrPis-t gene or not, so that toxic strains can be rapidly screened. The transposon insertion of an AvrPis-t promoter region, an upstream regulation region and a coding region can be more comprehensively detected, avirulent gene function loss caused by transposon insertion variation is accurately identified, and higher compatibility and reliability are achieved.

The invention belongs to the field of biological detection, and particularly relates to an RPA-LFD triple detection method and kit for rice blast, rice false smut and rhizoctonia solani and application of the RPA-LFD triple detection method and kit. The detection method comprises the following steps: S.1, extracting DNA of a strain and a sample; s.2, designing primers and probes: designing corresponding RPA primers and probes according to specific target sequences of genome DNA of rice blast, rice false smut and rhizoctonia solani; and S.3, measuring the sensitivity of a multiple RPA-LFD reaction system. By adopting multiple RPA and designing multiple groups of specific primers and probes, synchronous detection of three pathogens can be completed in a single tube at the same time, so that the detection efficiency is improved; the detection flux of the multiple RPA-LFD technology is improved by more than 3 times, and the reagent consumption is reduced by 60%.

The application belongs to the field of biological detection, and particularly relates to a RPA-LFD triple detection method and kit for rice blast, rice smut and sheath blight bacteria and application thereof. The detection method comprises the following steps: S.1 extraction of strain and sample DNA; S.2 design of primer and probe: corresponding RPA primers and probes are designed according to specific target sequences of rice blast, rice smut and sheath blight bacteria genomic DNA; S.3 sensitivity determination of multiplex RPA-LFD reaction system. The application can simultaneously complete synchronous detection of three kinds of pathogens in a single tube by designing multiple specific primers and probes through multiplex RPA, thereby improving detection efficiency; the detection throughput of multiplex RPA-LFD technology is improved by more than 3 times, and reagent consumption is reduced by 60%.

The application discloses a monkeypox virus whole genome capturing method, a primer group and a kit. Four groups of specific primer pools are designed for the monkeypox virus genome, and a high-fidelity enzyme system with high sustained synthesis capability is matched to carry out targeted capture on the monkeypox virus nucleic acid, so that the whole length of the monkeypox virus genome can be effectively amplified, and the application prospect is wide.

The invention discloses a fiveleaf virus whole genome capturing method, a primer group and a kit. Eight groups of specific primer pools are designed for the genome of the fiveleaf viruses, the GI type, the GII type, the GIV type and the GV type of the fiveleaf viruses can be effectively amplified, amplification products have high specificity and fidelity, and a new direction is provided for effective detection of the fiveleaf viruses.

The invention belongs to the technical field of molecular markers, and particularly relates to a multiple fluorescent quantitative PCR (polymerase chain reaction) detection method and a kit for distinguishing Chinese pangolin, manis pentadactyla and South African pangolin varieties. Three groups of specific primers and probes are utilized to distinguish different varieties of pangolin scales, including Chinese pangolin scales, malayan pangolin scales and South African pangolin scales. Specific gene segments of three species are amplified at the same time through one qPCR reaction, and the method has the advantages of being high in specificity and sensitivity, easy and convenient to operate and low in cost. The method is suitable for pangolin species identification, forensic identification, customs examination and biological protection research.

This invention belongs to the field of molecular biology detection technology for plant fungi, and discloses a LAMP primer set, reagent kit, and detection method for detecting the pathogen of oat smut. Based on the pathogen of oat smut... Ustilago hordei A set of LAMP-specific primers was designed based on specific sequences from the whole genome. The results were determined using real-time quantitative PCR, agarose gel electrophoresis, and SYBR Green I fluorescent dye assay to detect the oat smut pathogen carried on oat seeds. The LAMP detection method for oat smut pathogen established in this invention is highly specific, sensitive, fast, and low-cost, providing a new technical means for detecting oat smut pathogen carried on oat seeds and possessing high practical application value.

The invention discloses a monkey pox virus whole genome capturing method, a primer group and a kit. The four groups of specific primer pools are designed for the monkey pox virus genome, and are matched with the high-fidelity enzyme system with high continuous synthesis capacity to perform targeted capture on the monkey pox virus nucleic acid, so that the full length of the monkey pox genome can be effectively amplified, and the application prospect is wide.

The application discloses a nucleic acid detection kit and method for simultaneously identifying Brucella virulent strains and vaccine strains, wherein the Brucella virulent strains are bovine Brucella (B. Brucella abortus ), ovine Brucella (B. Brucella melitensis ) and porcine Brucella (B. Brucella suis ) virulent strains; the vaccine strains are A19 vaccine strains and S2 vaccine strains; the kit comprises five groups of specific primer pairs and corresponding detection probes, and target genes are ASU (bovine specific), ADZ (ovine specific), WZP (porcine specific), CP (conserved in bovine, ovine and porcine, A19 deletion gene) and WP (conserved in bovine, ovine and porcine, S2 deletion gene). Whether a sample with a known immune background is infected with a Brucella wild strain can be determined, and typing can be performed; for a sample with an unknown immune background, whether the sample is infected with a Brucella wild strain can be determined through double-tube quadruple fluorescence quantitative PCR detection. The experimental period is shortened, and the detection efficiency is improved.