31results about How to "Improved conservatism" patented technology

The invention relates to the technical field of molecular biology detection, in particular to a TaqMan-MGB fluorescent quantitative polymerase chain reaction (PCR) detection specific primer (as shown in SEQ ID No. 1 and SEQ ID No. 2), a TaqMan-MGB fluorescent quantitative PCR detection probe as shown in SEQ ID No. 3) and a TaqMan-MGB fluorescent quantitative PCR detection method for seneca valley virus (SVV). The method comprises the steps of drawing a standard curve; extracting ribonucleic acid (RNA) of a sample virus; carrying out reverse transcription on the RNA of the sample virus; enabling the product of the reverse transcription to have a TaqMan-MGB fluorescent quantitative PCR, and reading the result. The primer provided by the invention has better specificity and sensitivity; the MGB probe provided by the invention is shorter and is beneficial to probe design, and the Tm value difference between a paired template and a non-paired template is improved, so that the experimental result is more stable and accurate; the method provided by the invention has the advantages of being simple and rapid, easy to operate, visual in results, high in sensitivity, good in stability, real-time quantitative, and the like, and shortens the reaction time.

The present invention discloses an electric power system transient stability analysis method. The electric power system transient stability analysis method mainly comprises a time domain simulation method and a direct method. A transient energy function method belongs to the direct method. The transient energy function method model is simple, considers the non-linear problem, can avoid complex numerical integration operation, is fast in computing speed and can provide system stability margin indexes, and the restriction of the model precision, critical point selectivity and linearity path hypothesis allows a traditional transient energy function to always have the conservation problem. The improved transient energy function method based on the SMR (Squared Matrix Representation) technology introduces the SMR technology to improve the transient energy function analysis method, a superposition program is employed to obtain the optimal Lyapunov function so as to improve the problem of a traditional transient energy function conservation.

The invention provides a hybrid energy storage system capacity optimization configuration method based on a statistical model and belongs to the technical field of energy storage. The invention provides the configuration method based on the statistical model so as to solve the problem of energy storage capacity configuration of a wind/photovoltaic/energy storage combined power generation system. The method comprises the following steps: to begin with, carrying out probabilistic statistical analysis on historical data of wind speed and illumination intensity and the like to determine accurate distribution of power output of a wind and photovoltaic system; then, exploring a capacity configuration sub-algorithm based on a super capacitor and a storage battery; besides, providing an energy control strategy to improve storage battery operation environment so as to prolong the service life of the storage battery; and carrying out Monte-Carlo simulation on the statistical analysis to obtain capacity probability distribution of the hybrid energy storage system. The statistical analysis method determines the capacity of the hybrid energy storage system under different cumulative probability levels, and helps planning personnel to carry out reasonable configuration on the capacity of the hybrid energy storage system according to running conditions and requirements of the power generation system; and compared with a conventional capacity configuration method, the statistical analysis method takes a lot of uncertainty factors in the configuration process into consideration, thereby improving conservation of a conventional deterministic algorithm and improving economy and reliability of the configuration method.

The present invention relates to a method of preparing aseptic packaged cooked rice with black bean in an aseptic packing system by washing, dipping and dewatering black rhynchosia nolubilis and then mixing heat treated rhynchosia nolubilis, and in particular to a method of preparing aseptic packaged cooked rice with black been in an aseptic packing system capable of conserving the cooked rice with black been with excellent taste and incense for a long time at a normal temperature by improving conservative property. More specifically, the present invention relates to a method of preparing aseptic packaged cooked rice in an aseptic packing system that can conserve the cooked rice for a long time exceeding at least six months at a normal temperature and is excellent in safety against microorganisms, the method comprising the steps of: sorting uniform-sized black rhynchosia nolubilis through a size sorting operation; washing the black rhynchosia nolubilis so as not to allow their shells to be peeled off; dipping the washed rhynchosia nolubilis into water of 5 to 25° C. and dewatering them; leaving the washed black beans at a cold store of 10° C. or less for one hour; putting the black rhynchosia nolubilis of 15 mm or less in thickness into a packing material and sterilizing them for about 20 to 50 minutes at a temperature of 110 to 130° C.; mixing the sterilized rhynchosia nolubilis with nonglutinous rice at a ratio of 5 to 30% and automatically filling a predetermined amount of the mixture of rhynchosia nolubilis and nonglutinous rice into a heat-resistant plastic container; repeatedly performing high temperature/high pressure sterilization on the mixture of rhynchosia nolubilis and nonglutinous rice four to ten times for 4 to 9 seconds at a temperature of 130 to 150° C. in an sealed space; filling a predetermined amount of water for cooked rice and cooking rice at certain conditions; sealing and packing it in a aseptic space.

The invention provides a weak robustness optimized dispatching method for a micro energy network. The method comprises the following steps that: obtaining the architecture information, the energy price, the decision information, the user power and the renewable energy source output information of the micro energy network; according to the architecture information of the micro energy network, establishing the energy conversion and power balance model of the micro energy network; according to the energy price, the decision information and the energy conversion and power balance model of the of the micro energy network, establishing the weak robustness optimized dispatching model of the micro energy network; according to a peer-to-peer conversion theory, converting uncertainty constraints inthe weak robustness optimized dispatching model into certainty constraints; and according an optimal solution solved by GAMS (General Algebraic Modeling System) software, carrying out micro energy network dispatching. By use of the method, the problem of safe operation of the micro energy network under uncertainty parameters including user power, renewable energy source output and the like can besolved, dispatching economical efficiency and risks can be effectively balanced, the conservative property of traditional robustness optimized dispatching is improved, and a dispatching scheme which adapts to a practical situation can be more favorably provided.

The invention discloses a detection kit for novel coronavirus COVID-19 infection and belongs to the field of in-vitro diagnosis reagents. The kit disclosed by the invention comprises primers and probes for detecting a specific gene ORF1ab of novel coronavirus COVID-19, primers and probes for detecting a positive internal reference gene GAPDH and primers and probes for detecting an exogenous monitoring gene EGFP, and sequences are separately shown in SEQ ID No. 1-9. According to the kit, on the premise that the accuracy and stability of a detection result are guaranteed, the flux of detection is increased. According to the kit, a reagent Trizol is used as a sample preservation solution, safety of living beings, completeness of nucleic acid and load capacity of virus are guaranteed. According to the kit, RNA of the exogenous monitoring gene is introduced during RNA extraction, RNA loss conditions of a whole extraction and detection process can be monitored, the normativeness of a whole operating process is guaranteed, and false negative caused by defects of any step of the operating process is avoided.

The invention discloses a method for rapidly detecting 12 respiratory viruses by using a multiplex PCR technology and application of the method, and belongs to the technical field of gene engineering.In order to solve the problems of difficult biological classification and poor specificity of 12 respiratory viruses at present, genomic information of 12 respiratory viruses published by NCBI is analyzed to obtain genes of each respiratory virus with high specificity and good conservative property as target genes, and multiple primers and probes are designed according to the target genes, a multiplex PCR reaction system is obtained through optimization, the method capable of rapidly detecting the 12 respiratory viruses is obtained, and whether a sample contains the 12 respiratory viruses ornot can be rapidly detected through one-time PCR by means of the method.

The invention provides an epitope identification method of a thallus antibody, and belongs to the technical field of food safety immunological detection. A plurality of polypeptide fragments are obtained by affinity chromatography in identifying protein epitopes; intramembrane sequences and part of high-homology sequences in the target protein are preliminarily eliminated through topological structure, protein sequence homology and bacterial cross reaction analysis of the target protein; epitope mapping is mainly carried out on an extracellular loop structure sequence synthesis short peptide library with 3-6 amino acid differences, and tests prove that the method significantly reduces the workload and cost of epitope mapping, and improves the efficiency of epitope identification; the bacterial antigen epitope identification method has a wide application prospect in bacterial antibody epitope identification and exploration.

The present invention relates to a nested PCR detection method for equine piroplasmosis. The nested PCR detection method comprises the following steps: (1) conducting conservative analysis on 18S ribosomal RNA coding genes of two pathogens of the equine piroplasmosis and designing two pairs of primers by selecting a conservative region; (2) extracting DNA to be detected; (3) taking the DNA extracted in the step (2) as a template and preparing a PCR reaction system by using the outer primers designed in the step (1); and performing electrophoresis detection on a PCR product and performing secondary amplification if no target band exists in a 1,036 bp region; and (4) taking the first round nested PCR product in the step (3) as a template and preparing a PCR reaction system by using the outerprimers designed in the step (1); and performing electrophoresis detection on the PCR product, judging whether a target band appears in a 370 bp region, and if yes, judging the result positive. The method is relatively strong in specificity, relatively good in anti-interference performance, high in reliability and strong in practicability.

The invention provides a novel coronavirus detection kit as well as an application and use method thereof. The kit mainly comprises a primer probe mixture, wherein the primer probe mixture comprises Orf1ab gene, N gene and S gene specific forward and reverse primers and specific probes. The kit provided by the invention has the advantages of rapidness, high sensitivity, good specificity and good reproducibility. An amplified fragment in a primer sequence of an internal quality control product is gene in a human genome, so that the sensitivity of judging whether sampling is successful or not can be improved, a false negative detection result can be avoided, and potential patients can be isolated and treated in time. Research and development personnel accidentally discover that the S gene in the novel coronavirus has good conservative property in the research and development process, and has good specificity for detection of the novel coronavirus.